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The map label for this gene is hyuA [H]

Identifier: 134299737

GI number: 134299737

Start: 2071064

End: 2072746

Strand: Reverse

Name: hyuA [H]

Synonym: Dred_1886

Alternate gene names: 134299737

Gene position: 2072746-2071064 (Counterclockwise)

Preceding gene: 134299738

Following gene: 134299736

Centisome position: 57.45

GC content: 47.77

Gene sequence:

>1683_bases
ATGTTGATCGGTATAGATGTTGGCGGCACATGCACCGATGCTGTGTTGCTGGATGGCGGTGCTGTTTGTGCAACTGCCAA
GGTGGAAACCCAGGATGACTTATTAATTTCCCTGGGAGATGCACTGGATAACCTGCTAAAGGGAATACCCAGTCATAAGA
TAGAAAGAGTTGTCTTTAGTACAACTCTGGTTACAAATTTAATTGCAGAAAAGAAATATGACCCGGTTGGGGTCGTCATA
ATGCCTGGGCCCGGCAGAACTTTTTCAGACTTACGGGATCAGGACGATGTGTTTTGTTTAAATGGGGCCATTGATTATCG
CGGAAGAGAGATCATACCATTGCAACCTTCCCAAATGGAGGAGGTTCTTGCCTGGTTGGAAGCCAAGGGTTATAACAAGG
TGGCTGTGATCGGAAAGTTTTCCACTAGAAACAATAGCCACGAAGTAAAGGTCGCTGACTTCATAAACAAACATCATCCA
GACTGGCAGGTAGAAATGGGACATAGGGCTGGGGGAAAACTAAACTTTCCTCGGCGCATAGCCAATACGGTTCTAACCTG
TGCAACCCGCCAGAAGTATCGTTATTTTACTAAGGCTGTAAGGGATGCTATGGCGCGTAGAAATATAACGGCAACTGTTT
TTATCTTAAAAGCGGATGGGGGAACACTACCTCTTAGCGCTTCGGAAGAAGTTCCGGTAGAAACCATTTTTTCCGGACCT
GCTGCCAGTACCCTAGGCGTACAAGCCTTAACACCGCCGGGGGAAACCTCTTTGGTGGTGGATATTGGAGGGACCACCAC
GGATTTAGCTTTAGTTTTATCAGGTAATCCTTTGTTGGCTTCCAAGGGGGCAAAGATCGGAGAACGTTATACACAGGTCA
GAGCTTTAGCAGTGCATTCAGTTCCAGTGGGTGGGGATAGCGTTGTAGAAAGGGTAGGAAAAGAGTTTATTATTTACTCA
GAAAGAATGGGTCCTGCCTATTGCTTGGGGGGGCCCATGCCTACACCAACGGATGCCTTACGGGTCATGGGAATTAGCCA
AATTGGTGATGAGGATAGAGCCCACGAGGCAATGACTATCCTGGGAGCTCCCTTGGGCATGGGTGTCAGGGAAGTTGCCC
AGGCCATTGTAAATTTGGTTATTGACATGGTGGTTGTTGAAATAGAGCGAATGTATATGGAGTGGGAGCAAGAACCGGCT
TACCGTGTATGGGAGGTTATACAAAAGTGCAAAGTGCGCCCGCAAAATGTTGTGGGGGTAGGGGGAGGAGCCGCTGGTTT
TGTTCCTCAAATCGCTGCAAGATTAGGATGTACCCCTATATTGCCTCCCCATGCCCCGGTGGCCAATGCCATCGGAGCAG
CGGTTGCCCGGCCTACCTTACAAGTAAATCTAATGGCCGATACAGAGCGGCAGATTTACATTGTGGAGGAGGAGGGGTAC
CAGGGCCAATTAGCCGGCAAACTGGATGAACTCTCTGCCGTTGAACTGACAAAATCCTGGTTGTTAAAACGGGCTGAGAA
AATGAATTTGCGTAGTTATGTTGATGAAATAGAAATAATCAATCAGGATGTTTTTAATGTGGTTCGAGATTGGCAAACCA
CTGGCAGATTATTTCACATTTCGGTGCAAACACCCAGGGGGATTCTGGCTCATATCGGAACCGGGGGGAGGTTTTTACCA
TGA

Upstream 100 bases:

>100_bases
AGAATGTGTTAAACTGGAAATATGGGTCCATGCGGGAGAGTCAAGTGTTTGTTGACGAAGGATATGGTCCCTCTATAAAA
AATTAGTTAGGAGGCCCGTC

Downstream 100 bases:

>100_bases
GCAAAAAAACCGGCGTTATCTTTTTTCCTGCCTTCGATTGGGCAATTTCGCCAACGCACCCCGAACGGGAGGAGCGCCTC
CTTTATACCAGGGATCAAAT

Product: hydantoinase/oxoprolinase

Products: ADP; phosphate; N-carbamoylsarcosine

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 560; Mature: 560

Protein sequence:

>560_residues
MLIGIDVGGTCTDAVLLDGGAVCATAKVETQDDLLISLGDALDNLLKGIPSHKIERVVFSTTLVTNLIAEKKYDPVGVVI
MPGPGRTFSDLRDQDDVFCLNGAIDYRGREIIPLQPSQMEEVLAWLEAKGYNKVAVIGKFSTRNNSHEVKVADFINKHHP
DWQVEMGHRAGGKLNFPRRIANTVLTCATRQKYRYFTKAVRDAMARRNITATVFILKADGGTLPLSASEEVPVETIFSGP
AASTLGVQALTPPGETSLVVDIGGTTTDLALVLSGNPLLASKGAKIGERYTQVRALAVHSVPVGGDSVVERVGKEFIIYS
ERMGPAYCLGGPMPTPTDALRVMGISQIGDEDRAHEAMTILGAPLGMGVREVAQAIVNLVIDMVVVEIERMYMEWEQEPA
YRVWEVIQKCKVRPQNVVGVGGGAAGFVPQIAARLGCTPILPPHAPVANAIGAAVARPTLQVNLMADTERQIYIVEEEGY
QGQLAGKLDELSAVELTKSWLLKRAEKMNLRSYVDEIEIINQDVFNVVRDWQTTGRLFHISVQTPRGILAHIGTGGRFLP

Sequences:

>Translated_560_residues
MLIGIDVGGTCTDAVLLDGGAVCATAKVETQDDLLISLGDALDNLLKGIPSHKIERVVFSTTLVTNLIAEKKYDPVGVVI
MPGPGRTFSDLRDQDDVFCLNGAIDYRGREIIPLQPSQMEEVLAWLEAKGYNKVAVIGKFSTRNNSHEVKVADFINKHHP
DWQVEMGHRAGGKLNFPRRIANTVLTCATRQKYRYFTKAVRDAMARRNITATVFILKADGGTLPLSASEEVPVETIFSGP
AASTLGVQALTPPGETSLVVDIGGTTTDLALVLSGNPLLASKGAKIGERYTQVRALAVHSVPVGGDSVVERVGKEFIIYS
ERMGPAYCLGGPMPTPTDALRVMGISQIGDEDRAHEAMTILGAPLGMGVREVAQAIVNLVIDMVVVEIERMYMEWEQEPA
YRVWEVIQKCKVRPQNVVGVGGGAAGFVPQIAARLGCTPILPPHAPVANAIGAAVARPTLQVNLMADTERQIYIVEEEGY
QGQLAGKLDELSAVELTKSWLLKRAEKMNLRSYVDEIEIINQDVFNVVRDWQTTGRLFHISVQTPRGILAHIGTGGRFLP
>Mature_560_residues
MLIGIDVGGTCTDAVLLDGGAVCATAKVETQDDLLISLGDALDNLLKGIPSHKIERVVFSTTLVTNLIAEKKYDPVGVVI
MPGPGRTFSDLRDQDDVFCLNGAIDYRGREIIPLQPSQMEEVLAWLEAKGYNKVAVIGKFSTRNNSHEVKVADFINKHHP
DWQVEMGHRAGGKLNFPRRIANTVLTCATRQKYRYFTKAVRDAMARRNITATVFILKADGGTLPLSASEEVPVETIFSGP
AASTLGVQALTPPGETSLVVDIGGTTTDLALVLSGNPLLASKGAKIGERYTQVRALAVHSVPVGGDSVVERVGKEFIIYS
ERMGPAYCLGGPMPTPTDALRVMGISQIGDEDRAHEAMTILGAPLGMGVREVAQAIVNLVIDMVVVEIERMYMEWEQEPA
YRVWEVIQKCKVRPQNVVGVGGGAAGFVPQIAARLGCTPILPPHAPVANAIGAAVARPTLQVNLMADTERQIYIVEEEGY
QGQLAGKLDELSAVELTKSWLLKRAEKMNLRSYVDEIEIINQDVFNVVRDWQTTGRLFHISVQTPRGILAHIGTGGRFLP

Specific function: HyuA and hyuB convert D- and L-substituted hydantoins to corresponding N-carbamyl-amino acids [H]

COG id: COG0145

COG function: function code EQ; N-methylhydantoinase A/acetone carboxylase, beta subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the oxoprolinase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008040
- InterPro:   IPR002821 [H]

Pfam domain/function: PF05378 Hydant_A_N; PF01968 Hydantoinase_A [H]

EC number: 3.5.2.14

Molecular weight: Translated: 60694; Mature: 60694

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIGIDVGGTCTDAVLLDGGAVCATAKVETQDDLLISLGDALDNLLKGIPSHKIERVVFS
CEEEEECCCCCCCEEEECCCCEEEEEEECCCCCCEEEHHHHHHHHHHCCCCHHHHHHHHH
TTLVTNLIAEKKYDPVGVVIMPGPGRTFSDLRDQDDVFCLNGAIDYRGREIIPLQPSQME
HHHHHHHHHHCCCCCEEEEEECCCCCCHHHCCCCCCEEEEECEECCCCCEEECCCHHHHH
EVLAWLEAKGYNKVAVIGKFSTRNNSHEVKVADFINKHHPDWQVEMGHRAGGKLNFPRRI
HHHHHHHCCCCCEEEEEEEECCCCCCCEEEHHHHHHCCCCCEEEEECCCCCCCCCCCHHH
ANTVLTCATRQKYRYFTKAVRDAMARRNITATVFILKADGGTLPLSASEEVPVETIFSGP
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCCCCCCCHHEEECCC
AASTLGVQALTPPGETSLVVDIGGTTTDLALVLSGNPLLASKGAKIGERYTQVRALAVHS
CCCCCCEEEECCCCCCEEEEEECCCCEEEEEEEECCEEEECCCCHHHHHHHHHHHEEEEE
VPVGGDSVVERVGKEFIIYSERMGPAYCLGGPMPTPTDALRVMGISQIGDEDRAHEAMTI
CCCCCHHHHHHCCCEEEEEECCCCCEEEECCCCCCCHHHHHHHHHHHCCCCHHHHHHHHH
LGAPLGMGVREVAQAIVNLVIDMVVVEIERMYMEWEQEPAYRVWEVIQKCKVRPQNVVGV
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCEEEE
GGGAAGFVPQIAARLGCTPILPPHAPVANAIGAAVARPTLQVNLMADTERQIYIVEEEGY
CCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCEEEEEEEECCCCEEEEEECCCC
QGQLAGKLDELSAVELTKSWLLKRAEKMNLRSYVDEIEIINQDVFNVVRDWQTTGRLFHI
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEE
SVQTPRGILAHIGTGGRFLP
EECCCCCEEEEECCCCCCCC
>Mature Secondary Structure
MLIGIDVGGTCTDAVLLDGGAVCATAKVETQDDLLISLGDALDNLLKGIPSHKIERVVFS
CEEEEECCCCCCCEEEECCCCEEEEEEECCCCCCEEEHHHHHHHHHHCCCCHHHHHHHHH
TTLVTNLIAEKKYDPVGVVIMPGPGRTFSDLRDQDDVFCLNGAIDYRGREIIPLQPSQME
HHHHHHHHHHCCCCCEEEEEECCCCCCHHHCCCCCCEEEEECEECCCCCEEECCCHHHHH
EVLAWLEAKGYNKVAVIGKFSTRNNSHEVKVADFINKHHPDWQVEMGHRAGGKLNFPRRI
HHHHHHHCCCCCEEEEEEEECCCCCCCEEEHHHHHHCCCCCEEEEECCCCCCCCCCCHHH
ANTVLTCATRQKYRYFTKAVRDAMARRNITATVFILKADGGTLPLSASEEVPVETIFSGP
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCCCCCCCHHEEECCC
AASTLGVQALTPPGETSLVVDIGGTTTDLALVLSGNPLLASKGAKIGERYTQVRALAVHS
CCCCCCEEEECCCCCCEEEEEECCCCEEEEEEEECCEEEECCCCHHHHHHHHHHHEEEEE
VPVGGDSVVERVGKEFIIYSERMGPAYCLGGPMPTPTDALRVMGISQIGDEDRAHEAMTI
CCCCCHHHHHHCCCEEEEEECCCCCEEEECCCCCCCHHHHHHHHHHHCCCCHHHHHHHHH
LGAPLGMGVREVAQAIVNLVIDMVVVEIERMYMEWEQEPAYRVWEVIQKCKVRPQNVVGV
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCEEEE
GGGAAGFVPQIAARLGCTPILPPHAPVANAIGAAVARPTLQVNLMADTERQIYIVEEEGY
CCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCEEEEEEEECCCCEEEEEECCCC
QGQLAGKLDELSAVELTKSWLLKRAEKMNLRSYVDEIEIINQDVFNVVRDWQTTGRLFHI
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEE
SVQTPRGILAHIGTGGRFLP
EECCCCCEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; N-methylimidazolidine-2,4-dione; H2O

Specific reaction: ATP + N-methylimidazolidine-2,4-dione + 2 H2O = ADP + phosphate + N-carbamoylsarcosine

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1732229 [H]