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The map label for this gene is uraA [H]

Identifier: 134299180

GI number: 134299180

Start: 1409782

End: 1411050

Strand: Reverse

Name: uraA [H]

Synonym: Dred_1317

Alternate gene names: 134299180

Gene position: 1411050-1409782 (Counterclockwise)

Preceding gene: 134299181

Following gene: 134299178

Centisome position: 39.11

GC content: 44.6

Gene sequence:

>1269_bases
ATGGAGAAACGTGAAATCCAGGTAGAAGAAAGACTCCCCTTACTCCAAACCTTGCCCCTGAGTCTTCAGCATTTATTTGC
AATGTTTGGCGCTACAGTTCTGGTACCCATCCTGTTCAAGGTGGACCCTGCAACAATTCTACTATTTAACGGTATAGGAA
CACTCCTGTATATTTTTATCTGCAAAGGTCGTATCCCAGCATACCTGGGTTCAAGTTTTGCTTTTATATCCCCGGTAATT
GCAGTACTTAGTAAGTATGATTATAGCGCTGCCCTTGGCGGATTTATCGTAGTAGGCTTGATTTTCTCCGCAGTTGCCTT
GTTAATTGGGGCTGTGGGCACAAAATGGATTGATGTTGTCTTCCCACCTGCTGCCATGGGTGCTATTGTTGCAGTCATTG
GTTTAGAATTGGCACCTGTTGCTGCCCAGATGGCCGGCTTGGCTGCTGCTGACCCAACTAAATATGTTGCGGATCCAAAG
GTGATTACCGTATCCATGTTTACTTTGGGCGTAACTATTTTAGGCAATATTGTGTTCCGAGGTTTCTTAGCCATTATCCC
GATCCTAGTGGGTGTCATCTCTGGTTACGTTCTGTCTATCTTTATGGGCCTGGTAAGCACCGAGGCCATTGCACAGGCCA
GTTGGTTTGCAGTTCCTACTTTCTACCGCCCTACCTTTGATTTAAATGCAATTATCATTATTATTCCAGCGGCTCTGGTT
GTTATCGTAGAACATGTTGGCCACCTCTTTGTAACCAGTAACATTGTCAGTCGTGATTTATCAAAGGATCCTGGATTGCA
CCGTTCATTACTGGGCAACGGTATTTCTACCATGCTTTCCGGTTTCTTTGGATCCACCCCCAACACCACCTATGGCGAAA
ACATTGGGGTTATGGCCCTGACCAAGGTTTACAGTACTTGGGTTATTGGCGGGGCCGCCATTCTTGCTATCATTCTTTCT
TTCGTAGGAAAATTATCCGCTGCCATTCAAAGTATACCCACACCAGTTATGGGTGGTGTATCCCTACTGCTTTTTGGTGT
AATCGCTGCATCTGGTATCCGTATGCTTGTAGAAGCCAAGGTTGATTATAGTAAAGCATCAAACTTAATATTAACGTCCA
TTGTCATGATCATCGGAGTTAGTGGTGCTCAAATTCAATTGGGTGCTGTTAGCCTAAAAGGAATGGGGCTGGCAACGGTG
GTGGCTATGATTTTAAGTCTCTTCTTCCGCTTGCTGGAACAAGCCAATATTAGCAATGAGAATAAATAA

Upstream 100 bases:

>100_bases
GATAGCAGGATAAGTATATTCCTATAGTTATTTAGCTCCTTACAGTACTCTGTAAGGAGCTAATTGTTTTTTAGGCATAT
TAACTAAGGAGGAATACTAA

Downstream 100 bases:

>100_bases
TGCTTTAAAATTACTGCTTTGAAAAATTTGTTATGGATAAGTAAAGTTTACACCACATAAATACGTGTGGTGTTTTTCTA
TCTTTTTTTAAGGGTTAATT

Product: uracil transporter

Products: Proton [Cytoplasm]; uracil [Cytoplasm] [C]

Alternate protein names: Uracil transporter [H]

Number of amino acids: Translated: 422; Mature: 422

Protein sequence:

>422_residues
MEKREIQVEERLPLLQTLPLSLQHLFAMFGATVLVPILFKVDPATILLFNGIGTLLYIFICKGRIPAYLGSSFAFISPVI
AVLSKYDYSAALGGFIVVGLIFSAVALLIGAVGTKWIDVVFPPAAMGAIVAVIGLELAPVAAQMAGLAAADPTKYVADPK
VITVSMFTLGVTILGNIVFRGFLAIIPILVGVISGYVLSIFMGLVSTEAIAQASWFAVPTFYRPTFDLNAIIIIIPAALV
VIVEHVGHLFVTSNIVSRDLSKDPGLHRSLLGNGISTMLSGFFGSTPNTTYGENIGVMALTKVYSTWVIGGAAILAIILS
FVGKLSAAIQSIPTPVMGGVSLLLFGVIAASGIRMLVEAKVDYSKASNLILTSIVMIIGVSGAQIQLGAVSLKGMGLATV
VAMILSLFFRLLEQANISNENK

Sequences:

>Translated_422_residues
MEKREIQVEERLPLLQTLPLSLQHLFAMFGATVLVPILFKVDPATILLFNGIGTLLYIFICKGRIPAYLGSSFAFISPVI
AVLSKYDYSAALGGFIVVGLIFSAVALLIGAVGTKWIDVVFPPAAMGAIVAVIGLELAPVAAQMAGLAAADPTKYVADPK
VITVSMFTLGVTILGNIVFRGFLAIIPILVGVISGYVLSIFMGLVSTEAIAQASWFAVPTFYRPTFDLNAIIIIIPAALV
VIVEHVGHLFVTSNIVSRDLSKDPGLHRSLLGNGISTMLSGFFGSTPNTTYGENIGVMALTKVYSTWVIGGAAILAIILS
FVGKLSAAIQSIPTPVMGGVSLLLFGVIAASGIRMLVEAKVDYSKASNLILTSIVMIIGVSGAQIQLGAVSLKGMGLATV
VAMILSLFFRLLEQANISNENK
>Mature_422_residues
MEKREIQVEERLPLLQTLPLSLQHLFAMFGATVLVPILFKVDPATILLFNGIGTLLYIFICKGRIPAYLGSSFAFISPVI
AVLSKYDYSAALGGFIVVGLIFSAVALLIGAVGTKWIDVVFPPAAMGAIVAVIGLELAPVAAQMAGLAAADPTKYVADPK
VITVSMFTLGVTILGNIVFRGFLAIIPILVGVISGYVLSIFMGLVSTEAIAQASWFAVPTFYRPTFDLNAIIIIIPAALV
VIVEHVGHLFVTSNIVSRDLSKDPGLHRSLLGNGISTMLSGFFGSTPNTTYGENIGVMALTKVYSTWVIGGAAILAIILS
FVGKLSAAIQSIPTPVMGGVSLLLFGVIAASGIRMLVEAKVDYSKASNLILTSIVMIIGVSGAQIQLGAVSLKGMGLATV
VAMILSLFFRLLEQANISNENK

Specific function: Transport of uracil in the cell [H]

COG id: COG2233

COG function: function code F; Xanthine/uracil permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the xanthine/uracil permease family. Nucleobase:cation symporter-2 (NCS2) (TC 2.A.40) subfamily [H]

Homologues:

Organism=Homo sapiens, GI44680148, Length=442, Percent_Identity=26.6968325791855, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI40316845, Length=442, Percent_Identity=26.6968325791855, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI44680145, Length=450, Percent_Identity=23.7777777777778, Blast_Score=77, Evalue=4e-14,
Organism=Homo sapiens, GI44680143, Length=450, Percent_Identity=24.4444444444444, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI1788843, Length=432, Percent_Identity=66.2037037037037, Blast_Score=558, Evalue=1e-160,
Organism=Escherichia coli, GI87081818, Length=418, Percent_Identity=39.4736842105263, Blast_Score=282, Evalue=2e-77,
Organism=Escherichia coli, GI87082181, Length=456, Percent_Identity=28.0701754385965, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI1790087, Length=446, Percent_Identity=27.5784753363229, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI87082178, Length=371, Percent_Identity=24.5283018867925, Blast_Score=69, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI17558856, Length=181, Percent_Identity=28.1767955801105, Blast_Score=75, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI17541904, Length=226, Percent_Identity=26.5486725663717, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17542262, Length=176, Percent_Identity=27.2727272727273, Blast_Score=68, Evalue=9e-12,
Organism=Drosophila melanogaster, GI21356175, Length=172, Percent_Identity=31.3953488372093, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006042
- InterPro:   IPR006043 [H]

Pfam domain/function: PF00860 Xan_ur_permease [H]

EC number: NA

Molecular weight: Translated: 44404; Mature: 44404

Theoretical pI: Translated: 9.18; Mature: 9.18

Prosite motif: PS01116 XANTH_URACIL_PERMASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKREIQVEERLPLLQTLPLSLQHLFAMFGATVLVPILFKVDPATILLFNGIGTLLYIFI
CCCCCCCHHHCCCHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHEEEHHHHHHHHHHHH
CKGRIPAYLGSSFAFISPVIAVLSKYDYSAALGGFIVVGLIFSAVALLIGAVGTKWIDVV
HCCCCHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHEE
FPPAAMGAIVAVIGLELAPVAAQMAGLAAADPTKYVADPKVITVSMFTLGVTILGNIVFR
ECHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHH
GFLAIIPILVGVISGYVLSIFMGLVSTEAIAQASWFAVPTFYRPTFDLNAIIIIIPAALV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHH
VIVEHVGHLFVTSNIVSRDLSKDPGLHRSLLGNGISTMLSGFFGSTPNTTYGENIGVMAL
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCHHHH
TKVYSTWVIGGAAILAIILSFVGKLSAAIQSIPTPVMGGVSLLLFGVIAASGIRMLVEAK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
VDYSKASNLILTSIVMIIGVSGAQIQLGAVSLKGMGLATVVAMILSLFFRLLEQANISNE
CCHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCC
NK
CC
>Mature Secondary Structure
MEKREIQVEERLPLLQTLPLSLQHLFAMFGATVLVPILFKVDPATILLFNGIGTLLYIFI
CCCCCCCHHHCCCHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHEEEHHHHHHHHHHHH
CKGRIPAYLGSSFAFISPVIAVLSKYDYSAALGGFIVVGLIFSAVALLIGAVGTKWIDVV
HCCCCHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHEE
FPPAAMGAIVAVIGLELAPVAAQMAGLAAADPTKYVADPKVITVSMFTLGVTILGNIVFR
ECHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHH
GFLAIIPILVGVISGYVLSIFMGLVSTEAIAQASWFAVPTFYRPTFDLNAIIIIIPAALV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHH
VIVEHVGHLFVTSNIVSRDLSKDPGLHRSLLGNGISTMLSGFFGSTPNTTYGENIGVMAL
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCHHHH
TKVYSTWVIGGAAILAIILSFVGKLSAAIQSIPTPVMGGVSLLLFGVIAASGIRMLVEAK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
VDYSKASNLILTSIVMIIGVSGAQIQLGAVSLKGMGLATVVAMILSLFFRLLEQANISNE
CCHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCC
NK
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; uracil [Periplasm] [C]

Specific reaction: Proton [Periplasm] + uracil [Periplasm] = Proton [Cytoplasm] + uracil [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]