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The map label for this gene is fliY [H]

Identifier: 134298611

GI number: 134298611

Start: 796389

End: 797171

Strand: Reverse

Name: fliY [H]

Synonym: Dred_0743

Alternate gene names: 134298611

Gene position: 797171-796389 (Counterclockwise)

Preceding gene: 134298612

Following gene: 134298610

Centisome position: 22.09

GC content: 40.1

Gene sequence:

>783_bases
ATGTTTAATAAAAAGTCTATAGCCTTTTTGTTGACTCTCATTATAATTTCTACTTTGGTGGTTGGTTGCGGCGGTACATC
CAATTCTACCAGTGCCACACCGGATAAGAAAAAGTTTCATTATGCTATGAGTGGCCTATATAAACCTTTTAATTACAAAG
ATGGCGGTAAGTTGGTTGGTTTTGATGTGGAAATAGGCGAAGAAATAGCACGCAGAATTGGAATGGAAGCTGCTCCCGTT
ACCAACCCCTGGGAAACCATTATTCAGGGTTTGAAGGCTAACAAATACGATGCCATCATTGGTAGTATGGCCATTACAGA
AGAGCGCCAAGAACAGGTGGATTTCTCACGTCCCTACTACCGTTCCGGTGCTCAGGTCTTTGTCAGCAGTAACAATGATT
CCATAACATCAGCCAAAGACCTGAAAGGCAAAAAAATTGGTGTTGTTAAAGCCAGTACCTTCCGTAATGTGGCTTTGGAG
TATACTGATAAGAAGAACGTGATTGGTTATGATAGCGATGTAATTGCCCTGCAGGATTTACCCACTGGTAGAGTGGATGC
TGTTATAACTGATCAAATGGTTGGTATCGTAGCTATAAAAAATGGCTTAAAGATCAAAGATGTTGATAAACCCCTCTGGG
TTGATGAAATGGCCATTCCTGTTAATAAAGGGAATACCGAGTTGGTAAACAAGATTAATAACGCACTGGATGAAATGATC
AAAGATGGTACTTACGAAAAAATAAGTAATAAGTGGTTTGGTCGGAACATTTTAGGTGACTAG

Upstream 100 bases:

>100_bases
CATATAAAGTTGTCTTGTAATGTTTTACAGAAGCGTCAAGTAAAGGAGGTCGAGTACTACAACTGCTTGGTTGACTAAAT
AAATATCGAGGAGGAAGAAA

Downstream 100 bases:

>100_bases
TATAGTTGGAGTTGGGTGCAACTTCTTGCACCCAACCACAGCTAAAGAGGTGAATGAATGACAGACATATATGGAATATT
CACAGAGAATTTTCTTGCCT

Product: extracellular solute-binding protein

Products: NA

Alternate protein names: CBP; Protein fliY; Sulfate starvation-induced protein 7; SSI7 [H]

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MFNKKSIAFLLTLIIISTLVVGCGGTSNSTSATPDKKKFHYAMSGLYKPFNYKDGGKLVGFDVEIGEEIARRIGMEAAPV
TNPWETIIQGLKANKYDAIIGSMAITEERQEQVDFSRPYYRSGAQVFVSSNNDSITSAKDLKGKKIGVVKASTFRNVALE
YTDKKNVIGYDSDVIALQDLPTGRVDAVITDQMVGIVAIKNGLKIKDVDKPLWVDEMAIPVNKGNTELVNKINNALDEMI
KDGTYEKISNKWFGRNILGD

Sequences:

>Translated_260_residues
MFNKKSIAFLLTLIIISTLVVGCGGTSNSTSATPDKKKFHYAMSGLYKPFNYKDGGKLVGFDVEIGEEIARRIGMEAAPV
TNPWETIIQGLKANKYDAIIGSMAITEERQEQVDFSRPYYRSGAQVFVSSNNDSITSAKDLKGKKIGVVKASTFRNVALE
YTDKKNVIGYDSDVIALQDLPTGRVDAVITDQMVGIVAIKNGLKIKDVDKPLWVDEMAIPVNKGNTELVNKINNALDEMI
KDGTYEKISNKWFGRNILGD
>Mature_260_residues
MFNKKSIAFLLTLIIISTLVVGCGGTSNSTSATPDKKKFHYAMSGLYKPFNYKDGGKLVGFDVEIGEEIARRIGMEAAPV
TNPWETIIQGLKANKYDAIIGSMAITEERQEQVDFSRPYYRSGAQVFVSSNNDSITSAKDLKGKKIGVVKASTFRNVALE
YTDKKNVIGYDSDVIALQDLPTGRVDAVITDQMVGIVAIKNGLKIKDVDKPLWVDEMAIPVNKGNTELVNKINNALDEMI
KDGTYEKISNKWFGRNILGD

Specific function: Part of a binding-protein-dependent transport system for cystine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788228, Length=262, Percent_Identity=29.3893129770992, Blast_Score=144, Evalue=6e-36,
Organism=Escherichia coli, GI1788649, Length=258, Percent_Identity=31.0077519379845, Blast_Score=129, Evalue=1e-31,
Organism=Escherichia coli, GI1787088, Length=248, Percent_Identity=34.2741935483871, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI1788648, Length=228, Percent_Identity=33.7719298245614, Blast_Score=124, Evalue=6e-30,
Organism=Escherichia coli, GI1787085, Length=223, Percent_Identity=33.1838565022422, Blast_Score=124, Evalue=8e-30,
Organism=Escherichia coli, GI1787031, Length=229, Percent_Identity=33.1877729257642, Blast_Score=112, Evalue=2e-26,

Paralogues:

None

Copy number: 1920 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1060 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 80 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 28627; Mature: 28627

Theoretical pI: Translated: 8.81; Mature: 8.81

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFNKKSIAFLLTLIIISTLVVGCGGTSNSTSATPDKKKFHYAMSGLYKPFNYKDGGKLVG
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEE
FDVEIGEEIARRIGMEAAPVTNPWETIIQGLKANKYDAIIGSMAITEERQEQVDFSRPYY
EEHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCC
RSGAQVFVSSNNDSITSAKDLKGKKIGVVKASTFRNVALEYTDKKNVIGYDSDVIALQDL
CCCCEEEEECCCCCCCCHHHCCCCEEEEEECCCCCCEEEEECCCCCCCCCCCCEEEEECC
PTGRVDAVITDQMVGIVAIKNGLKIKDVDKPLWVDEMAIPVNKGNTELVNKINNALDEMI
CCCCCCEEEHHHHEEEEEECCCCEEEECCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHH
KDGTYEKISNKWFGRNILGD
HCCCHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MFNKKSIAFLLTLIIISTLVVGCGGTSNSTSATPDKKKFHYAMSGLYKPFNYKDGGKLVG
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEE
FDVEIGEEIARRIGMEAAPVTNPWETIIQGLKANKYDAIIGSMAITEERQEQVDFSRPYY
EEHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCC
RSGAQVFVSSNNDSITSAKDLKGKKIGVVKASTFRNVALEYTDKKNVIGYDSDVIALQDL
CCCCEEEEECCCCCCCCHHHCCCCEEEEEECCCCCCEEEEECCCCCCCCCCCCEEEEECC
PTGRVDAVITDQMVGIVAIKNGLKIKDVDKPLWVDEMAIPVNKGNTELVNKINNALDEMI
CCCCCCEEEHHHHEEEEEECCCCEEEECCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHH
KDGTYEKISNKWFGRNILGD
HCCCHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]