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The map label for this gene is splB [H]

Identifier: 134298559

GI number: 134298559

Start: 745795

End: 746853

Strand: Reverse

Name: splB [H]

Synonym: Dred_0691

Alternate gene names: 134298559

Gene position: 746853-745795 (Counterclockwise)

Preceding gene: 134298566

Following gene: 134298530

Centisome position: 20.7

GC content: 45.33

Gene sequence:

>1059_bases
ATGAAACAGATCCTTTTGGAAAGACCCGCAGCCAACCATATCTTTATCCCTAAACGTGTTTTTATGGAACCAGCGGCCCT
GGAATATGAACTGGGTCAACGTTTAAGAGAGCAATTCAGAGCTGAGGGGATTCCTGTAACTGTCACTGGTTCCCATAACC
GAGTCACTGGTATACCCGGTACAACACCCCAAGAGGCTTTTCTGGAGTCGAAACGAACGCTGGTGGTAGGTGTGCGCAGG
GGAAAGGATTTTCAAACCTGTAAACCCTCGGCCCATTACCAGCTTCCCTTGGTAACCAGTTGTCCTGGCAAATGCGAATA
TTGTTATCTTTTAACCAATCTAGGAAAAAAACCTTACATCCGGTTGTATGTAAATATTGAAGAAATATTGGACCGGGCCA
AAGAATACATAATCGAATATTTGCCCCGGGAAACGATCTTTGAAGGAGCTGCTACATCAGATCCCATTCCTGTAGAACAC
TATACCGGTGCTTTAAAACAAGCCATTGAATTTTTTGGTGGTCAGGAGCACGCCCGCTTCCGCTTTGTAACTAAGTTTAG
TGATGTCGATTCCTTGTTGGATGCTAAACATAACGGACGTACTCGTTTTCGCTTTAGTTTAAACACGGATTATGTCATTA
GACAATTTGAACACGGTACTCCACCCATGCATGAACGGGTTGCGGCAGCGGGGAAAGTAGCCCAAGCAGGTTATCCCCTG
GGGTTCCTGGTGGCTCCCATTGTGCTTTATGACGGCTGGCAACAGGGCTACCAGGAGCTTTTTGAAGAGCTGTACCGAGT
GCTGGACCTTGTATCCCAGCAAGACTTAACCTTTGAGTTTATAACCCACCGTTTTACCCAGCGAGCCAAAACCAATATTC
TGGATATCTTTCCCAACTGCCGTCTGGATATGGATGAGGAAAAAAGGCAATTTAAATTCGGTCAATTTGGTTACGGTAAG
TATGTTTATCCCAAGGATAGTATGGCAGAGGTTAAGGAATTCATGCAAGGCATGGTGGATAGGTACTTCCCTGCCGCTAA
AGTAGAGTATTTGGTATAA

Upstream 100 bases:

>100_bases
ATATTTAAATTGGATCCAGAGTCCTACTTTATTTTGATTAGAGCATAGCAAGAAAAACTTTGAGACAAACTGATACAAAT
TGATTAAAGGAAGAATGCTA

Downstream 100 bases:

>100_bases
GATTTAAGTTTGCTTATTGCAAGGAGGTATTTGTTTATGAAATGTCCCGAAGACTCCCAATGCCTTACCTGTGTAGGTAC
TACCTGTACCTGCGGTGACT

Product: spore photoproduct lyase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 352; Mature: 352

Protein sequence:

>352_residues
MKQILLERPAANHIFIPKRVFMEPAALEYELGQRLREQFRAEGIPVTVTGSHNRVTGIPGTTPQEAFLESKRTLVVGVRR
GKDFQTCKPSAHYQLPLVTSCPGKCEYCYLLTNLGKKPYIRLYVNIEEILDRAKEYIIEYLPRETIFEGAATSDPIPVEH
YTGALKQAIEFFGGQEHARFRFVTKFSDVDSLLDAKHNGRTRFRFSLNTDYVIRQFEHGTPPMHERVAAAGKVAQAGYPL
GFLVAPIVLYDGWQQGYQELFEELYRVLDLVSQQDLTFEFITHRFTQRAKTNILDIFPNCRLDMDEEKRQFKFGQFGYGK
YVYPKDSMAEVKEFMQGMVDRYFPAAKVEYLV

Sequences:

>Translated_352_residues
MKQILLERPAANHIFIPKRVFMEPAALEYELGQRLREQFRAEGIPVTVTGSHNRVTGIPGTTPQEAFLESKRTLVVGVRR
GKDFQTCKPSAHYQLPLVTSCPGKCEYCYLLTNLGKKPYIRLYVNIEEILDRAKEYIIEYLPRETIFEGAATSDPIPVEH
YTGALKQAIEFFGGQEHARFRFVTKFSDVDSLLDAKHNGRTRFRFSLNTDYVIRQFEHGTPPMHERVAAAGKVAQAGYPL
GFLVAPIVLYDGWQQGYQELFEELYRVLDLVSQQDLTFEFITHRFTQRAKTNILDIFPNCRLDMDEEKRQFKFGQFGYGK
YVYPKDSMAEVKEFMQGMVDRYFPAAKVEYLV
>Mature_352_residues
MKQILLERPAANHIFIPKRVFMEPAALEYELGQRLREQFRAEGIPVTVTGSHNRVTGIPGTTPQEAFLESKRTLVVGVRR
GKDFQTCKPSAHYQLPLVTSCPGKCEYCYLLTNLGKKPYIRLYVNIEEILDRAKEYIIEYLPRETIFEGAATSDPIPVEH
YTGALKQAIEFFGGQEHARFRFVTKFSDVDSLLDAKHNGRTRFRFSLNTDYVIRQFEHGTPPMHERVAAAGKVAQAGYPL
GFLVAPIVLYDGWQQGYQELFEELYRVLDLVSQQDLTFEFITHRFTQRAKTNILDIFPNCRLDMDEEKRQFKFGQFGYGK
YVYPKDSMAEVKEFMQGMVDRYFPAAKVEYLV

Specific function: Involved in repair of UV radiation-induced DNA damage during spore germination. Can repair thymine dimer 5-thyminyl-5,6- dihydrothymine (known as spore photoproduct (SP)) by in situ monomerization of SP to two thymines [H]

COG id: COG1533

COG function: function code L; DNA repair photolyase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. SPL family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR004594 [H]

Pfam domain/function: NA

EC number: =4.1.99.14 [H]

Molecular weight: Translated: 40660; Mature: 40660

Theoretical pI: Translated: 7.91; Mature: 7.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQILLERPAANHIFIPKRVFMEPAALEYELGQRLREQFRAEGIPVTVTGSHNRVTGIPG
CCCCCCCCCCCCCEECCHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEECCCC
TTPQEAFLESKRTLVVGVRRGKDFQTCKPSAHYQLPLVTSCPGKCEYCYLLTNLGKKPYI
CCHHHHHHHCCCEEEEEEECCCCCCCCCCCCCEECEEEECCCCCCCEEEEEHHCCCCCEE
RLYVNIEEILDRAKEYIIEYLPRETIFEGAATSDPIPVEHYTGALKQAIEFFGGQEHARF
EEEEEHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEE
RFVTKFSDVDSLLDAKHNGRTRFRFSLNTDYVIRQFEHGTPPMHERVAAAGKVAQAGYPL
EEEEHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCH
GFLVAPIVLYDGWQQGYQELFEELYRVLDLVSQQDLTFEFITHRFTQRAKTNILDIFPNC
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCEEECCCC
RLDMDEEKRQFKFGQFGYGKYVYPKDSMAEVKEFMQGMVDRYFPAAKVEYLV
CCCCCHHHHHCCCCCCCCCCEECCCCCHHHHHHHHHHHHHHCCCCCCEEECC
>Mature Secondary Structure
MKQILLERPAANHIFIPKRVFMEPAALEYELGQRLREQFRAEGIPVTVTGSHNRVTGIPG
CCCCCCCCCCCCCEECCHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEECCCC
TTPQEAFLESKRTLVVGVRRGKDFQTCKPSAHYQLPLVTSCPGKCEYCYLLTNLGKKPYI
CCHHHHHHHCCCEEEEEEECCCCCCCCCCCCCEECEEEECCCCCCCEEEEEHHCCCCCEE
RLYVNIEEILDRAKEYIIEYLPRETIFEGAATSDPIPVEHYTGALKQAIEFFGGQEHARF
EEEEEHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEE
RFVTKFSDVDSLLDAKHNGRTRFRFSLNTDYVIRQFEHGTPPMHERVAAAGKVAQAGYPL
EEEEHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCH
GFLVAPIVLYDGWQQGYQELFEELYRVLDLVSQQDLTFEFITHRFTQRAKTNILDIFPNC
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCEEECCCC
RLDMDEEKRQFKFGQFGYGKYVYPKDSMAEVKEFMQGMVDRYFPAAKVEYLV
CCCCCHHHHHCCCCCCCCCCEECCCCCHHHHHHHHHHHHHHCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8449881; 9384377; 9353924 [H]