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The map label for this gene is rocR [H]

Identifier: 134298451

GI number: 134298451

Start: 623601

End: 625085

Strand: Reverse

Name: rocR [H]

Synonym: Dred_0580

Alternate gene names: 134298451

Gene position: 625085-623601 (Counterclockwise)

Preceding gene: 134298456

Following gene: 134298436

Centisome position: 17.32

GC content: 36.36

Gene sequence:

>1485_bases
ATGACCAAAAACAACCTGTTAAAATCTCTAATAAACTTTAACGCAGCTGAAACAAGTTGTAAAAATGTCAGTGCCGATTA
CGACTTGGTTATTAATTCCTTAGATTGTTTGGAAGATTGTTATTTTTCAATAGTTGACGCAACAGGTAAAATTATTTTCG
TGTCAAAAGATTTTGAAAAAATAGATGGTTACAAAATTAGCGATATATTGGGGAAAAATGTATTGGATGTTTACAAATTG
GGTAAACAAAATAGTGTACATGTAAGATCAATAGCTGAAAAAAAGGTATTGAAAAATACTAAACTAAGATATACCTCAGC
CTCTGGTCAACCTGTTGACCTCGTAATGGATATATATCCGGTTTTTTCGGGACGAGAGGTAATTGGTTCATTTGCCATAA
GTCGAGACACAAGCAAAATACAAGCACTTACAGACAAAGTATTTCAACTTCAAAAAGCCCTTTATAATCAACTACAGAGA
ACGAACAATAACGGAACCCAGTTTTGTTTTGATGACATCATTGGTTCTGGAGAAGCCATGCAAAGCGTAATTAATACAGC
GAAAAAAATGGCCTGCTCGGAATCACGGATCATGATTCTGGGAGAAACAGGAACAGGCAAAGAGCTCTTTGCCCAGAGTA
TTCATAACAACAGCTCAAGAGCCAAAGAGCCTTTTATCGCGGTTAACTGTTCAGCCATTCCAGATACCTTATTAGAAAGT
ATCCTGTTTGGGACAAATAAAGGTGCATTTACCGGAGCTGAGGATAAAGCCGGGTTGTTCGAAGAAGCAAAAAATGGCAC
ACTTTTTCTGGACGAACTTAATTCTATGAGTATCGTTCTCCAGTCCAAACTATTAAGAGCATTGGAAACGAATCGCATTC
GCAGGGTGGGAGGTAACAAAGAAATCCCTGTAAACCCTCGAATTATTAGTGCTATGAATATTGACCCTAAAGAAGCCATT
GAAACTGAAAAACTAAGGAGTGATTTTTATTACAGACTCGCTGTGATCACTTTAGAATGTCCACCTTTACGAAACAGAAA
GGAAGATATCATTACCTTATCCTGGTTTTACATCAATAAATATAATAAGATTCTGGGGAGAAAAATTAACGAGATATCCG
AAGAAGTAATTCAAATACTGGAAAACTATAACTGGCCAGGAAATGTTCGCGAATTAAGTCATTGTATCGAGCATGCGATG
AACATAGTTGATCCTACTGATACTTCCCTGTTAGTGCAGCATTTACCCGCGTTCCTCCAGGACAAAATCCTCAAGAATAA
TATTTTGCCGATCACCGTGCCAAAAATTAACGATTATAAACAAATAATGCTGCAAGTAGAAAAAGACCTCTTGACACAAG
CACTAAAAAGAAATACCGGCAATATTAACCAAACCGCCAAAGAATTAAATCTGTCCAGACAATGTTTATATTATAAGCTG
AAACGATTAGATATCGACATTCAGCATGAGATTTATATAGAATAA

Upstream 100 bases:

>100_bases
AAATAAGTTAAAATATAGACAAGATAAGTGAACATTTTCACAGCAATAGTTTCAGTATTTTTTAAAATTAACATAAATGA
CATTTAAGGAGATGACATAG

Downstream 100 bases:

>100_bases
TTTGCTAAGGATGAGAGATTTCTAGCTACTATTTAAAATCAATGAGGGGTTAGGCCTGATAATTATCAAACCTGACCCCT
TTGAAGTTACCCTTAAGGTT

Product: sigma-54 dependent trancsriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 494; Mature: 493

Protein sequence:

>494_residues
MTKNNLLKSLINFNAAETSCKNVSADYDLVINSLDCLEDCYFSIVDATGKIIFVSKDFEKIDGYKISDILGKNVLDVYKL
GKQNSVHVRSIAEKKVLKNTKLRYTSASGQPVDLVMDIYPVFSGREVIGSFAISRDTSKIQALTDKVFQLQKALYNQLQR
TNNNGTQFCFDDIIGSGEAMQSVINTAKKMACSESRIMILGETGTGKELFAQSIHNNSSRAKEPFIAVNCSAIPDTLLES
ILFGTNKGAFTGAEDKAGLFEEAKNGTLFLDELNSMSIVLQSKLLRALETNRIRRVGGNKEIPVNPRIISAMNIDPKEAI
ETEKLRSDFYYRLAVITLECPPLRNRKEDIITLSWFYINKYNKILGRKINEISEEVIQILENYNWPGNVRELSHCIEHAM
NIVDPTDTSLLVQHLPAFLQDKILKNNILPITVPKINDYKQIMLQVEKDLLTQALKRNTGNINQTAKELNLSRQCLYYKL
KRLDIDIQHEIYIE

Sequences:

>Translated_494_residues
MTKNNLLKSLINFNAAETSCKNVSADYDLVINSLDCLEDCYFSIVDATGKIIFVSKDFEKIDGYKISDILGKNVLDVYKL
GKQNSVHVRSIAEKKVLKNTKLRYTSASGQPVDLVMDIYPVFSGREVIGSFAISRDTSKIQALTDKVFQLQKALYNQLQR
TNNNGTQFCFDDIIGSGEAMQSVINTAKKMACSESRIMILGETGTGKELFAQSIHNNSSRAKEPFIAVNCSAIPDTLLES
ILFGTNKGAFTGAEDKAGLFEEAKNGTLFLDELNSMSIVLQSKLLRALETNRIRRVGGNKEIPVNPRIISAMNIDPKEAI
ETEKLRSDFYYRLAVITLECPPLRNRKEDIITLSWFYINKYNKILGRKINEISEEVIQILENYNWPGNVRELSHCIEHAM
NIVDPTDTSLLVQHLPAFLQDKILKNNILPITVPKINDYKQIMLQVEKDLLTQALKRNTGNINQTAKELNLSRQCLYYKL
KRLDIDIQHEIYIE
>Mature_493_residues
TKNNLLKSLINFNAAETSCKNVSADYDLVINSLDCLEDCYFSIVDATGKIIFVSKDFEKIDGYKISDILGKNVLDVYKLG
KQNSVHVRSIAEKKVLKNTKLRYTSASGQPVDLVMDIYPVFSGREVIGSFAISRDTSKIQALTDKVFQLQKALYNQLQRT
NNNGTQFCFDDIIGSGEAMQSVINTAKKMACSESRIMILGETGTGKELFAQSIHNNSSRAKEPFIAVNCSAIPDTLLESI
LFGTNKGAFTGAEDKAGLFEEAKNGTLFLDELNSMSIVLQSKLLRALETNRIRRVGGNKEIPVNPRIISAMNIDPKEAIE
TEKLRSDFYYRLAVITLECPPLRNRKEDIITLSWFYINKYNKILGRKINEISEEVIQILENYNWPGNVRELSHCIEHAMN
IVDPTDTSLLVQHLPAFLQDKILKNNILPITVPKINDYKQIMLQVEKDLLTQALKRNTGNINQTAKELNLSRQCLYYKLK
RLDIDIQHEIYIE

Specific function: Positive regulator of arginine catabolism. Controls the transcription of the two operons rocABC and rocDEF and probably acts by binding to the corresponding upstream activating sequences [H]

COG id: COG3829

COG function: function code KT; Transcriptional regulator containing PAS, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=349, Percent_Identity=38.6819484240688, Blast_Score=244, Evalue=1e-65,
Organism=Escherichia coli, GI87082117, Length=328, Percent_Identity=39.0243902439024, Blast_Score=228, Evalue=6e-61,
Organism=Escherichia coli, GI1789087, Length=372, Percent_Identity=39.247311827957, Blast_Score=224, Evalue=8e-60,
Organism=Escherichia coli, GI1790437, Length=342, Percent_Identity=35.0877192982456, Blast_Score=223, Evalue=2e-59,
Organism=Escherichia coli, GI1789233, Length=324, Percent_Identity=38.8888888888889, Blast_Score=222, Evalue=3e-59,
Organism=Escherichia coli, GI1790299, Length=350, Percent_Identity=35.4285714285714, Blast_Score=205, Evalue=4e-54,
Organism=Escherichia coli, GI1786524, Length=373, Percent_Identity=34.0482573726542, Blast_Score=203, Evalue=2e-53,
Organism=Escherichia coli, GI1788905, Length=339, Percent_Identity=35.9882005899705, Blast_Score=203, Evalue=2e-53,
Organism=Escherichia coli, GI87082152, Length=318, Percent_Identity=36.4779874213836, Blast_Score=202, Evalue=4e-53,
Organism=Escherichia coli, GI87081872, Length=323, Percent_Identity=34.6749226006192, Blast_Score=184, Evalue=1e-47,
Organism=Escherichia coli, GI1787583, Length=376, Percent_Identity=32.4468085106383, Blast_Score=182, Evalue=4e-47,
Organism=Escherichia coli, GI87081858, Length=458, Percent_Identity=24.8908296943231, Blast_Score=128, Evalue=1e-30,
Organism=Escherichia coli, GI1789828, Length=259, Percent_Identity=29.3436293436293, Blast_Score=100, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR000014
- InterPro:   IPR013656
- InterPro:   IPR002078 [H]

Pfam domain/function: PF02954 HTH_8; PF08448 PAS_4; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 55856; Mature: 55725

Theoretical pI: Translated: 7.92; Mature: 7.92

Prosite motif: PS50112 PAS ; PS00675 SIGMA54_INTERACT_1 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKNNLLKSLINFNAAETSCKNVSADYDLVINSLDCLEDCYFSIVDATGKIIFVSKDFEK
CCHHHHHHHHHCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHH
IDGYKISDILGKNVLDVYKLGKQNSVHVRSIAEKKVLKNTKLRYTSASGQPVDLVMDIYP
HCCEEHHHHHCCHHHHHHHCCCCCCCHHHHHHHHHHHHCCCEEEECCCCCCCEEHEEHHH
VFSGREVIGSFAISRDTSKIQALTDKVFQLQKALYNQLQRTNNNGTQFCFDDIIGSGEAM
CCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHCCCCHHH
QSVINTAKKMACSESRIMILGETGTGKELFAQSIHNNSSRAKEPFIAVNCSAIPDTLLES
HHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHH
ILFGTNKGAFTGAEDKAGLFEEAKNGTLFLDELNSMSIVLQSKLLRALETNRIRRVGGNK
HHHCCCCCCCCCCCCCCCHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCC
EIPVNPRIISAMNIDPKEAIETEKLRSDFYYRLAVITLECPPLRNRKEDIITLSWFYINK
CCCCCCCEEEEECCCHHHHHHHHHHHHHHEEEEEEEEEECCCCCCCCCCEEEEEEEEHHH
YNKILGRKINEISEEVIQILENYNWPGNVRELSHCIEHAMNIVDPTDTSLLVQHLPAFLQ
HHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
DKILKNNILPITVPKINDYKQIMLQVEKDLLTQALKRNTGNINQTAKELNLSRQCLYYKL
HHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCHHHHHHHHHH
KRLDIDIQHEIYIE
HHCCEEECEEEEEC
>Mature Secondary Structure 
TKNNLLKSLINFNAAETSCKNVSADYDLVINSLDCLEDCYFSIVDATGKIIFVSKDFEK
CHHHHHHHHHCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHH
IDGYKISDILGKNVLDVYKLGKQNSVHVRSIAEKKVLKNTKLRYTSASGQPVDLVMDIYP
HCCEEHHHHHCCHHHHHHHCCCCCCCHHHHHHHHHHHHCCCEEEECCCCCCCEEHEEHHH
VFSGREVIGSFAISRDTSKIQALTDKVFQLQKALYNQLQRTNNNGTQFCFDDIIGSGEAM
CCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHCCCCHHH
QSVINTAKKMACSESRIMILGETGTGKELFAQSIHNNSSRAKEPFIAVNCSAIPDTLLES
HHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHH
ILFGTNKGAFTGAEDKAGLFEEAKNGTLFLDELNSMSIVLQSKLLRALETNRIRRVGGNK
HHHCCCCCCCCCCCCCCCHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCC
EIPVNPRIISAMNIDPKEAIETEKLRSDFYYRLAVITLECPPLRNRKEDIITLSWFYINK
CCCCCCCEEEEECCCHHHHHHHHHHHHHHEEEEEEEEEECCCCCCCCCCEEEEEEEEHHH
YNKILGRKINEISEEVIQILENYNWPGNVRELSHCIEHAMNIVDPTDTSLLVQHLPAFLQ
HHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
DKILKNNILPITVPKINDYKQIMLQVEKDLLTQALKRNTGNINQTAKELNLSRQCLYYKL
HHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCHHHHHHHHHH
KRLDIDIQHEIYIE
HHCCEEECEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8113162; 9384377 [H]