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The map label for this gene is pfl [H]

Identifier: 134297918

GI number: 134297918

Start: 45889

End: 48120

Strand: Reverse

Name: pfl [H]

Synonym: Dred_0039

Alternate gene names: 134297918

Gene position: 48120-45889 (Counterclockwise)

Preceding gene: 134297930

Following gene: 134297917

Centisome position: 1.33

GC content: 41.8

Gene sequence:

>2232_bases
TTGAAACAAGCATGGTCTGATTTTTTAGGATTTAAATGGCAACAGGAGATCAATGTACGTAATTTTATCCAATGCAATTA
CAAACCCTATGAAGGAAAAAGTAATTTTCTCTGTGAACAAACTCCAAAAACCAAACAACTATGGGAAAAATGCCTAAAAC
TATTCCAGGCAGAAAGAAAAAATAATGGTGTGTTAAGTATCAATACCAGTCGGGCCACAGGAATTAATACCTTTGGACCA
GGTTTTATTGACCGTGATTTGGAATTGATTTTTGGTTTGCAAACAGAAAAGCCTCTGGAACGTTCTGTGAACCCCTTTGG
CGGCTTGCGGATGGCCAAGGAATCCTGTAAAGCCTATGGTTACTCCTTAGACAAACAGTTAGAAGATTTTTTTGTTAAAT
ATAAAAAGACGCATAACGATGGGGTTTTTGATGCCTATACTCCAGAAATGCGTCTGGCTAGAAGTCTTGGCATTATTACT
GGTCTGCCAGATGCCTATGGACGTGGCAGAATCATCGGGGATTACCGGCGGGTTCCTCTATACGGAGTTGATCGCTTAAT
TGAAGAAAAAGAAAAGGATCTGGCTAATATGCCAACACACACCCTTACTTCCCAAGCCATTCAACTCCGGGAAGAAATTA
GGGAGCAGATTAAATCCCTAGTTGATTTAAAAGAGATGGCCAGACAATATGGTTTTGACATCGGTTCTCCGGCGGCCAAT
GCAAAGGAAGCCTTTCAATGGCTATACTTTGCTTATCTTGGTGCCATTAAAGAACAAAACGGTGCCGCCATGTCTTTGGG
CCGAGTCAGTACGTTTTTAGATATCTACATTGAGAGAGACATTGAGGCGGGAGTTTTGCAAGAAACAGAGGCCCAGGAAT
TGGTTGACCAGTTTGTTATTAAACTTCGCTTAGCCAGACAATTGAGAACTCCGGAATATGATAAATTGTTCGCCGGGGAT
CCTATGTGGATTACTGAAGCCATTGGTGGCATGGGCTTAGATGGCAGAACCTTGGTTTCAAAAAATTCCTATCGTTTTCT
ACAAACTTTAATCAATCTGGGACCTGCGCCAGAACCTAACTTAACAATTCTTTGGTCAGACCGTTTGCCGGAGAATTTTA
AAAAATTCAGTGCCCAAATATCTATTAAAACCAGTTCCATTCAATATGAAAATGATGACTTAATGAGAGAGTATTACGGT
GATGATTATGGCATTGCCTGCTGTGTTTCTGCCATGAAAATAGGCAAACAAATGCAGTTCTTTGGGGCCAGGGCTAATTT
AGCAAAACTGTTGTTATTAGCTATCAACGGTGGCCAAGACGAAATGACAGGTAAACAACTGGCACCGCCCATTCCTTCCT
TGGATGATGACTATCTGGATTACGAGAAGGTTATGAAAAACTTTCTACTGTTACAGGATTGGCTGTGCAAATTGTATGTA
AATACCCTGAACATTATTCACTTTATGCATGATAAATATGCCTACGAAAAAATCCAACTGGCCTTGCATGATAGCGACGT
AGAAAGACTGATGGCCTTTGGTATGGCAGGATTGTCGGTGGTTGCAGACTCCCTTAGCGCCATTAAATATGCTAAGGTGT
ACCCTATCCGAAATGAAGAAGGAATTGCTGTGGATTTTAGGATTGAGGGGGATTTTCCTAAATACGGCAATGATGACGAT
CGGGTGGATGATATTGCGGTGGCACTGGTACGTAGTTTTAATAATAATTTGCAAAAATACACAACTTACCGCAACGCCAA
ACATACACTATCCATCTTAACCATTACCTCCAATGTTATGTATGGTAAAAAAACCGGTACTACGCCGGATGGGCGGCAAA
AGGGTGAGCCCTTTGCACCGGGAGCTACTCCCATGCACGGCAGGGATGTGAACGGCGCCTTGGCAAGCCTTAATTCTGTG
GCAAAACTTCCCTACAATTCATCTCTTGACGGTATTAGCAATACCTTCTCCATTGTTCCCGCTGCCCTGGGGAAAACCCA
TGAAGACAGGGAAGCTAACCTAGTCAACATTTTAAGGGGTTATTTTAATCAACAAGCCCATCATTTAAATGTCAATGTTC
TGGATCGTACCACATTGGAAAGGGCGATGGAAGAACCGGAAAACTATCCACAATTAACAGTCCGAGTTTCCGGCTATGCC
GTTCACTTTACCAAGTTAAGCAGAGCTCATCAGCTGGAAGTTATTAAAAGGACTTTTTTTGAAAGATTTTAA

Upstream 100 bases:

>100_bases
CAAAGGATTGTAACTGTTTTTTAAGCCGACCGCCTGGGCAATACACGTTTCTGTGTTATTGTTCCAGGCGGTCTTTGTTT
TTAGAAAGGAGATGGTTTCA

Downstream 100 bases:

>100_bases
ATAAAGGGGCTGTTAAACATGAAGGGAAGAATACACTCCTTTGAAAGCTGTGGTACGGTGGATGGGCCGGGGATTCGTTG
TGTGGTGTTTTTTCAAGGCT

Product: formate acetyltransferase

Products: NA

Alternate protein names: Pyruvate formate-lyase [H]

Number of amino acids: Translated: 743; Mature: 743

Protein sequence:

>743_residues
MKQAWSDFLGFKWQQEINVRNFIQCNYKPYEGKSNFLCEQTPKTKQLWEKCLKLFQAERKNNGVLSINTSRATGINTFGP
GFIDRDLELIFGLQTEKPLERSVNPFGGLRMAKESCKAYGYSLDKQLEDFFVKYKKTHNDGVFDAYTPEMRLARSLGIIT
GLPDAYGRGRIIGDYRRVPLYGVDRLIEEKEKDLANMPTHTLTSQAIQLREEIREQIKSLVDLKEMARQYGFDIGSPAAN
AKEAFQWLYFAYLGAIKEQNGAAMSLGRVSTFLDIYIERDIEAGVLQETEAQELVDQFVIKLRLARQLRTPEYDKLFAGD
PMWITEAIGGMGLDGRTLVSKNSYRFLQTLINLGPAPEPNLTILWSDRLPENFKKFSAQISIKTSSIQYENDDLMREYYG
DDYGIACCVSAMKIGKQMQFFGARANLAKLLLLAINGGQDEMTGKQLAPPIPSLDDDYLDYEKVMKNFLLLQDWLCKLYV
NTLNIIHFMHDKYAYEKIQLALHDSDVERLMAFGMAGLSVVADSLSAIKYAKVYPIRNEEGIAVDFRIEGDFPKYGNDDD
RVDDIAVALVRSFNNNLQKYTTYRNAKHTLSILTITSNVMYGKKTGTTPDGRQKGEPFAPGATPMHGRDVNGALASLNSV
AKLPYNSSLDGISNTFSIVPAALGKTHEDREANLVNILRGYFNQQAHHLNVNVLDRTTLERAMEEPENYPQLTVRVSGYA
VHFTKLSRAHQLEVIKRTFFERF

Sequences:

>Translated_743_residues
MKQAWSDFLGFKWQQEINVRNFIQCNYKPYEGKSNFLCEQTPKTKQLWEKCLKLFQAERKNNGVLSINTSRATGINTFGP
GFIDRDLELIFGLQTEKPLERSVNPFGGLRMAKESCKAYGYSLDKQLEDFFVKYKKTHNDGVFDAYTPEMRLARSLGIIT
GLPDAYGRGRIIGDYRRVPLYGVDRLIEEKEKDLANMPTHTLTSQAIQLREEIREQIKSLVDLKEMARQYGFDIGSPAAN
AKEAFQWLYFAYLGAIKEQNGAAMSLGRVSTFLDIYIERDIEAGVLQETEAQELVDQFVIKLRLARQLRTPEYDKLFAGD
PMWITEAIGGMGLDGRTLVSKNSYRFLQTLINLGPAPEPNLTILWSDRLPENFKKFSAQISIKTSSIQYENDDLMREYYG
DDYGIACCVSAMKIGKQMQFFGARANLAKLLLLAINGGQDEMTGKQLAPPIPSLDDDYLDYEKVMKNFLLLQDWLCKLYV
NTLNIIHFMHDKYAYEKIQLALHDSDVERLMAFGMAGLSVVADSLSAIKYAKVYPIRNEEGIAVDFRIEGDFPKYGNDDD
RVDDIAVALVRSFNNNLQKYTTYRNAKHTLSILTITSNVMYGKKTGTTPDGRQKGEPFAPGATPMHGRDVNGALASLNSV
AKLPYNSSLDGISNTFSIVPAALGKTHEDREANLVNILRGYFNQQAHHLNVNVLDRTTLERAMEEPENYPQLTVRVSGYA
VHFTKLSRAHQLEVIKRTFFERF
>Mature_743_residues
MKQAWSDFLGFKWQQEINVRNFIQCNYKPYEGKSNFLCEQTPKTKQLWEKCLKLFQAERKNNGVLSINTSRATGINTFGP
GFIDRDLELIFGLQTEKPLERSVNPFGGLRMAKESCKAYGYSLDKQLEDFFVKYKKTHNDGVFDAYTPEMRLARSLGIIT
GLPDAYGRGRIIGDYRRVPLYGVDRLIEEKEKDLANMPTHTLTSQAIQLREEIREQIKSLVDLKEMARQYGFDIGSPAAN
AKEAFQWLYFAYLGAIKEQNGAAMSLGRVSTFLDIYIERDIEAGVLQETEAQELVDQFVIKLRLARQLRTPEYDKLFAGD
PMWITEAIGGMGLDGRTLVSKNSYRFLQTLINLGPAPEPNLTILWSDRLPENFKKFSAQISIKTSSIQYENDDLMREYYG
DDYGIACCVSAMKIGKQMQFFGARANLAKLLLLAINGGQDEMTGKQLAPPIPSLDDDYLDYEKVMKNFLLLQDWLCKLYV
NTLNIIHFMHDKYAYEKIQLALHDSDVERLMAFGMAGLSVVADSLSAIKYAKVYPIRNEEGIAVDFRIEGDFPKYGNDDD
RVDDIAVALVRSFNNNLQKYTTYRNAKHTLSILTITSNVMYGKKTGTTPDGRQKGEPFAPGATPMHGRDVNGALASLNSV
AKLPYNSSLDGISNTFSIVPAALGKTHEDREANLVNILRGYFNQQAHHLNVNVLDRTTLERAMEEPENYPQLTVRVSGYA
VHFTKLSRAHQLEVIKRTFFERF

Specific function: Glucose metabolism (nonoxidative conversion). [C]

COG id: COG1882

COG function: function code C; Pyruvate-formate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pyruvate formate lyase domain [H]

Homologues:

Organism=Escherichia coli, GI1787131, Length=749, Percent_Identity=58.7449933244326, Blast_Score=919, Evalue=0.0,
Organism=Escherichia coli, GI48994926, Length=746, Percent_Identity=59.3833780160858, Blast_Score=915, Evalue=0.0,
Organism=Escherichia coli, GI1787044, Length=588, Percent_Identity=26.7006802721088, Blast_Score=173, Evalue=3e-44,
Organism=Escherichia coli, GI1790388, Length=714, Percent_Identity=26.890756302521, Blast_Score=155, Evalue=1e-38,
Organism=Escherichia coli, GI1788933, Length=57, Percent_Identity=61.4035087719298, Blast_Score=74, Evalue=5e-14,

Paralogues:

None

Copy number: 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005949
- InterPro:   IPR001150
- InterPro:   IPR019777
- InterPro:   IPR004184 [H]

Pfam domain/function: PF01228 Gly_radical; PF02901 PFL [H]

EC number: =2.3.1.54 [H]

Molecular weight: Translated: 84368; Mature: 84368

Theoretical pI: Translated: 7.13; Mature: 7.13

Prosite motif: PS00850 GLY_RADICAL_1 ; PS51149 GLY_RADICAL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQAWSDFLGFKWQQEINVRNFIQCNYKPYEGKSNFLCEQTPKTKQLWEKCLKLFQAERK
CCCCHHHHHCCCCHHCCCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHC
NNGVLSINTSRATGINTFGPGFIDRDLELIFGLQTEKPLERSVNPFGGLRMAKESCKAYG
CCCEEEEECCCCCCCCCCCCCCCCCCHHHEEECCCCCCHHHCCCCCCHHHHHHHHHHHHC
YSLDKQLEDFFVKYKKTHNDGVFDAYTPEMRLARSLGIITGLPDAYGRGRIIGDYRRVPL
CCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCH
YGVDRLIEEKEKDLANMPTHTLTSQAIQLREEIREQIKSLVDLKEMARQYGFDIGSPAAN
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
AKEAFQWLYFAYLGAIKEQNGAAMSLGRVSTFLDIYIERDIEAGVLQETEAQELVDQFVI
HHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHH
KLRLARQLRTPEYDKLFAGDPMWITEAIGGMGLDGRTLVSKNSYRFLQTLINLGPAPEPN
HHHHHHHHCCCCCCCEECCCCHHHHHHHCCCCCCCCEEECCCHHHHHHHHHHCCCCCCCC
LTILWSDRLPENFKKFSAQISIKTSSIQYENDDLMREYYGDDYGIACCVSAMKIGKQMQF
EEEEECCCCCHHHHHHEEEEEEEECEEEECCHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
FGARANLAKLLLLAINGGQDEMTGKQLAPPIPSLDDDYLDYEKVMKNFLLLQDWLCKLYV
HCCHHHHHEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
NTLNIIHFMHDKYAYEKIQLALHDSDVERLMAFGMAGLSVVADSLSAIKYAKVYPIRNEE
HHHHHHHHHHHHHHHHHHEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCC
GIAVDFRIEGDFPKYGNDDDRVDDIAVALVRSFNNNLQKYTTYRNAKHTLSILTITSNVM
CEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHCCCCCEEEEEEEECCEE
YGKKTGTTPDGRQKGEPFAPGATPMHGRDVNGALASLNSVAKLPYNSSLDGISNTFSIVP
ECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
AALGKTHEDREANLVNILRGYFNQQAHHLNVNVLDRTTLERAMEEPENYPQLTVRVSGYA
HHHCCCCCCCHHHHHHHHHHHHCCCCEEEEEEEEHHHHHHHHHHCCCCCCEEEEEEECEE
VHFTKLSRAHQLEVIKRTFFERF
EEHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKQAWSDFLGFKWQQEINVRNFIQCNYKPYEGKSNFLCEQTPKTKQLWEKCLKLFQAERK
CCCCHHHHHCCCCHHCCCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHC
NNGVLSINTSRATGINTFGPGFIDRDLELIFGLQTEKPLERSVNPFGGLRMAKESCKAYG
CCCEEEEECCCCCCCCCCCCCCCCCCHHHEEECCCCCCHHHCCCCCCHHHHHHHHHHHHC
YSLDKQLEDFFVKYKKTHNDGVFDAYTPEMRLARSLGIITGLPDAYGRGRIIGDYRRVPL
CCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCH
YGVDRLIEEKEKDLANMPTHTLTSQAIQLREEIREQIKSLVDLKEMARQYGFDIGSPAAN
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
AKEAFQWLYFAYLGAIKEQNGAAMSLGRVSTFLDIYIERDIEAGVLQETEAQELVDQFVI
HHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHH
KLRLARQLRTPEYDKLFAGDPMWITEAIGGMGLDGRTLVSKNSYRFLQTLINLGPAPEPN
HHHHHHHHCCCCCCCEECCCCHHHHHHHCCCCCCCCEEECCCHHHHHHHHHHCCCCCCCC
LTILWSDRLPENFKKFSAQISIKTSSIQYENDDLMREYYGDDYGIACCVSAMKIGKQMQF
EEEEECCCCCHHHHHHEEEEEEEECEEEECCHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
FGARANLAKLLLLAINGGQDEMTGKQLAPPIPSLDDDYLDYEKVMKNFLLLQDWLCKLYV
HCCHHHHHEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
NTLNIIHFMHDKYAYEKIQLALHDSDVERLMAFGMAGLSVVADSLSAIKYAKVYPIRNEE
HHHHHHHHHHHHHHHHHHEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCC
GIAVDFRIEGDFPKYGNDDDRVDDIAVALVRSFNNNLQKYTTYRNAKHTLSILTITSNVM
CEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHCCCCCEEEEEEEECCEE
YGKKTGTTPDGRQKGEPFAPGATPMHGRDVNGALASLNSVAKLPYNSSLDGISNTFSIVP
ECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
AALGKTHEDREANLVNILRGYFNQQAHHLNVNVLDRTTLERAMEEPENYPQLTVRVSGYA
HHHCCCCCCCHHHHHHHHHHHHCCCCEEEEEEEEHHHHHHHHHHCCCCCCEEEEEEECEE
VHFTKLSRAHQLEVIKRTFFERF
EEHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8636053 [H]