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The map label for this gene is act [H]

Identifier: 134297917

GI number: 134297917

Start: 45181

End: 45870

Strand: Reverse

Name: act [H]

Synonym: Dred_0038

Alternate gene names: 134297917

Gene position: 45870-45181 (Counterclockwise)

Preceding gene: 134297918

Following gene: 134297913

Centisome position: 1.27

GC content: 42.9

Gene sequence:

>690_bases
ATGAAGGGAAGAATACACTCCTTTGAAAGCTGTGGTACGGTGGATGGGCCGGGGATTCGTTGTGTGGTGTTTTTTCAAGG
CTGTTTACTAAGGTGCCGCTATTGTCATAATCCCGATACCTGGGATCTGCTAGGTGGTCAGGAAATGGACAGCGATGAAA
TCGTAAAAAAGGTACGTCGTTTTAAATCCTACTTTCACAACAACGGTGGCATTACCCTATCCGGCGGGGAACCACTCTTG
CAGCCTGATTTTGCTTTTGCTATTCTGCAGCAATGTAAAAAAGAAGGTATCCACACAGCGGTGGATACCTCCGGTTGTAT
CGATGTCGGCGCTTTGGAAAAGATTTTACCCTTTACCGATCTGCTTTTATTGGATGTTAAGGCTGTGGATGATAGCCTTT
ATCATTGGCTAACAGGAGGAAAGGCTGAAACATTTCAAATGGCTGTTGATTATATTCGCCAGCAAAAAACTCCTCTCTGG
TTACGCTATGTGGTTCTACCAGGAATGAACGATTCTCCAGAATATAGGTATCGACTTGAGAAGCTTATAAACTCGCTGGG
TGATCAAGTAAAAAAAGTGGAGCTTTTGCCCTATCATACCATGGGAGTACACAAATGGAAAAAACTTGGTTTAGTCTACC
CTTTAAATAACTTAAAACCAGCTACAGCAAGCACCATTGCCAATTTTTAA

Upstream 100 bases:

>100_bases
CGGCTATGCCGTTCACTTTACCAAGTTAAGCAGAGCTCATCAGCTGGAAGTTATTAAAAGGACTTTTTTTGAAAGATTTT
AAATAAAGGGGCTGTTAAAC

Downstream 100 bases:

>100_bases
CCCCACCATGGGGTTATTTTTTCTTTGCACCACATAATGCTTAAAATTATTTTACCAACTTTTTACCCCTACTGTTACCA
TTCTTATTACTGGTCCTTTT

Product: pyruvate formate-lyase activating enzyme

Products: NA

Alternate protein names: PFL-activating enzyme; Formate-C-acetyltransferase-activating enzyme [H]

Number of amino acids: Translated: 229; Mature: 229

Protein sequence:

>229_residues
MKGRIHSFESCGTVDGPGIRCVVFFQGCLLRCRYCHNPDTWDLLGGQEMDSDEIVKKVRRFKSYFHNNGGITLSGGEPLL
QPDFAFAILQQCKKEGIHTAVDTSGCIDVGALEKILPFTDLLLLDVKAVDDSLYHWLTGGKAETFQMAVDYIRQQKTPLW
LRYVVLPGMNDSPEYRYRLEKLINSLGDQVKKVELLPYHTMGVHKWKKLGLVYPLNNLKPATASTIANF

Sequences:

>Translated_229_residues
MKGRIHSFESCGTVDGPGIRCVVFFQGCLLRCRYCHNPDTWDLLGGQEMDSDEIVKKVRRFKSYFHNNGGITLSGGEPLL
QPDFAFAILQQCKKEGIHTAVDTSGCIDVGALEKILPFTDLLLLDVKAVDDSLYHWLTGGKAETFQMAVDYIRQQKTPLW
LRYVVLPGMNDSPEYRYRLEKLINSLGDQVKKVELLPYHTMGVHKWKKLGLVYPLNNLKPATASTIANF
>Mature_229_residues
MKGRIHSFESCGTVDGPGIRCVVFFQGCLLRCRYCHNPDTWDLLGGQEMDSDEIVKKVRRFKSYFHNNGGITLSGGEPLL
QPDFAFAILQQCKKEGIHTAVDTSGCIDVGALEKILPFTDLLLLDVKAVDDSLYHWLTGGKAETFQMAVDYIRQQKTPLW
LRYVVLPGMNDSPEYRYRLEKLINSLGDQVKKVELLPYHTMGVHKWKKLGLVYPLNNLKPATASTIANF

Specific function: Activation of pyruvate formate-lyase under anaerobic conditions by generation of an organic free radical, using S- adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine [H]

COG id: COG1180

COG function: function code O; Pyruvate-formate lyase-activating enzyme

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the organic radical-activating enzymes family [H]

Homologues:

Organism=Escherichia coli, GI1787130, Length=227, Percent_Identity=44.0528634361233, Blast_Score=211, Evalue=4e-56,
Organism=Escherichia coli, GI1790839, Length=240, Percent_Identity=27.9166666666667, Blast_Score=85, Evalue=3e-18,
Organism=Escherichia coli, GI1790389, Length=259, Percent_Identity=23.1660231660232, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI226510931, Length=286, Percent_Identity=26.5734265734266, Blast_Score=73, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012838
- InterPro:   IPR001989
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =1.97.1.4 [H]

Molecular weight: Translated: 25869; Mature: 25869

Theoretical pI: Translated: 8.06; Mature: 8.06

Prosite motif: PS01087 RADICAL_ACTIVATING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGRIHSFESCGTVDGPGIRCVVFFQGCLLRCRYCHNPDTWDLLGGQEMDSDEIVKKVRR
CCCCCCCHHCCCCCCCCCCEEHHHHHHHHHHHHHCCCCCCEECCCCCCCCHHHHHHHHHH
FKSYFHNNGGITLSGGEPLLQPDFAFAILQQCKKEGIHTAVDTSGCIDVGALEKILPFTD
HHHHHHCCCCEEECCCCCCCCCHHHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHH
LLLLDVKAVDDSLYHWLTGGKAETFQMAVDYIRQQKTPLWLRYVVLPGMNDSPEYRYRLE
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHH
KLINSLGDQVKKVELLPYHTMGVHKWKKLGLVYPLNNLKPATASTIANF
HHHHHHHHHHHHHHCCCCHHHHHHHHHHCCEEECCCCCCCCHHHHHCCC
>Mature Secondary Structure
MKGRIHSFESCGTVDGPGIRCVVFFQGCLLRCRYCHNPDTWDLLGGQEMDSDEIVKKVRR
CCCCCCCHHCCCCCCCCCCEEHHHHHHHHHHHHHCCCCCCEECCCCCCCCHHHHHHHHHH
FKSYFHNNGGITLSGGEPLLQPDFAFAILQQCKKEGIHTAVDTSGCIDVGALEKILPFTD
HHHHHHCCCCEEECCCCCCCCCHHHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHH
LLLLDVKAVDDSLYHWLTGGKAETFQMAVDYIRQQKTPLWLRYVVLPGMNDSPEYRYRLE
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHH
KLINSLGDQVKKVELLPYHTMGVHKWKKLGLVYPLNNLKPATASTIANF
HHHHHHHHHHHHHHCCCCHHHHHHHHHHCCEEECCCCCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8636053 [H]