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The map label for this gene is aspS

Identifier: 126697217

GI number: 126697217

Start: 1605229

End: 1607025

Strand: Direct

Name: aspS

Synonym: P9301_18791

Alternate gene names: 126697217

Gene position: 1605229-1607025 (Clockwise)

Preceding gene: 126697216

Following gene: 126697218

Centisome position: 97.77

GC content: 32.05

Gene sequence:

>1797_bases
ATGAGAAACAAAATTTGCAAAGAACTCAATAATACAGATATTGGTAAATTAGTTAATTTATGCGGATGGGTAGATAGAAG
AAGAGATCATGGTGGTGTAATTTTTATTGATTTAAGAGACCATAGTGGATTCCTACAAATAACAATTAATCCCGATGATG
GTGCAGCTCTATTTAAACAGGCAGAAACTCTAAGAAATGAGACAGTAATAATGGTTAGCGGAATTATTAATGAAAGGCCC
AAAGATTCCATAAATACAAATTTAAGTACTGGAGAGTTAGAGCTTAAGGTTAAAGATTTGCAAATTCTCAACCAGATTAA
AAAAAACTTACCTTTTCCAGTGTCTATTCATGATTATGAAAATACAAAAGAAGAACTCAGATTAAAATATAGGTACCTTG
ATTTAAGAAGGGGAAAATTACTAGAAAATTTAAAAACAAGACATAAGATTATTAAAGTTGCTAGAGAATTTCTTGATAAT
TTTGGATTTACAGAAGTTGAGACTCCATTACTTACAAAATCAACTCCAGAAGGCGCACGCGATTTTCTTGTTCCTGCACG
TCTTTCAAATGGAGAATTTTTTGCTTTACCTCAATCCCCACAACTATTTAAACAACTTTTAATGGTTGGAGGCCTGGACA
AGTATTATCAAATCGCAAAATGTTTCCGTGATGAAGACTTAAGGGCAGATAGACAGCCAGAGTTTACTCAATTAGATATT
GAGATGAGCTTTATTAGTGAAGAAGAAATAATTTCTTTTAATGAAAGTCTCATAAAAAAAATATGGAAAGAAGTTTTAAA
TATTAAATTTAATAATGCTTTTCCAAGAATGTCATGGCAGGAAGCAATGGATAATTATGGCACTGATAGACCAGATACTA
GATATCAAATGTTATTAAAAGATTTAGGAGGAATATTAGGTGATATTGGATTTAATATTTTTACCAAGGCAATTAAGTCT
GGAGGTTATATAAAATCCATAACAGTAAAAGGAGGTAATTCAAGTATTAGCAACGTAAGAATTAAACCAGGTGGTGATAT
CTTCCAAGTAGCTAAAGATGCAGGAGCTGGAGGTTTGGCCTTTATAAGGGTTAAAGGAGATGAGCTTGAGACTATTGGGG
CAATTAAAAATAATTTAAGTGAAGAGCATATAGCTGATATTTTAAAAATCACAGAAGCAAAAGATGGAGACTTAATCCTT
TTAGGAGCTGGAGATAAACTAATTGTCAATCAGTCATTAGATAGGGTCAGACAATATATCGCAAAAGACTTAAATCTCAT
TGATAAAAGTAAATGGAATTTCTTATGGGTAACTGACTTCCCGATGTTTGAGAGAAATGAAGAGGAAAATAGATATGAAG
CTTTGCATCATCCTTTTTGTTCTCCAAAAAATATAAAATCAAAAGATTCTGAAAACTTGAAAAAAGAAATTGAGAGCTCT
ACAGCAAATGCTTATGACTTAGTTCTTAATGGATTGGAGTTAGGAGGTGGCTCTTTACGTATTCATGAAGCGAACTTACA
AAGAGAGGTTCTTAAAATGGTAGGACTTACTGATAAAGAAATTGATGAAAAATTTGGATTTTTAATAGAAGCATTAGAAA
TGGGTGCTCCTCCTCATGGTGGAATAGCGTTTGGATTAGATCGTATTACCATGCTGATCATTGGTACAGATTCAATCAGA
GAAACAATTGCTTTTCCAAAAAATCAGCAAGCAAAATGTCTTCTCACAAATGCACCTTCAAATGTCTCTGAATCACAATT
AAAAGAATTAGATATTGAAATAACAATTGATGAATAA

Upstream 100 bases:

>100_bases
AAAGGGGTTATAACAAAAAGAGCGTTTTATAAAAAAAATTATTAACTAAATTTACCCTTGAAGAATTATTATAAGTTATT
GATAAATAAATCATACTTAG

Downstream 100 bases:

>100_bases
GAATATATATGGATGTACTAAAAGTTTTTTGTTTAAATAAAATATAAGGAGGCTGTGCTTAATTAAAAATATTTAATGTC
AAAATTTGTATTTGTCACCG

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS

Number of amino acids: Translated: 598; Mature: 598

Protein sequence:

>598_residues
MRNKICKELNNTDIGKLVNLCGWVDRRRDHGGVIFIDLRDHSGFLQITINPDDGAALFKQAETLRNETVIMVSGIINERP
KDSINTNLSTGELELKVKDLQILNQIKKNLPFPVSIHDYENTKEELRLKYRYLDLRRGKLLENLKTRHKIIKVAREFLDN
FGFTEVETPLLTKSTPEGARDFLVPARLSNGEFFALPQSPQLFKQLLMVGGLDKYYQIAKCFRDEDLRADRQPEFTQLDI
EMSFISEEEIISFNESLIKKIWKEVLNIKFNNAFPRMSWQEAMDNYGTDRPDTRYQMLLKDLGGILGDIGFNIFTKAIKS
GGYIKSITVKGGNSSISNVRIKPGGDIFQVAKDAGAGGLAFIRVKGDELETIGAIKNNLSEEHIADILKITEAKDGDLIL
LGAGDKLIVNQSLDRVRQYIAKDLNLIDKSKWNFLWVTDFPMFERNEEENRYEALHHPFCSPKNIKSKDSENLKKEIESS
TANAYDLVLNGLELGGGSLRIHEANLQREVLKMVGLTDKEIDEKFGFLIEALEMGAPPHGGIAFGLDRITMLIIGTDSIR
ETIAFPKNQQAKCLLTNAPSNVSESQLKELDIEITIDE

Sequences:

>Translated_598_residues
MRNKICKELNNTDIGKLVNLCGWVDRRRDHGGVIFIDLRDHSGFLQITINPDDGAALFKQAETLRNETVIMVSGIINERP
KDSINTNLSTGELELKVKDLQILNQIKKNLPFPVSIHDYENTKEELRLKYRYLDLRRGKLLENLKTRHKIIKVAREFLDN
FGFTEVETPLLTKSTPEGARDFLVPARLSNGEFFALPQSPQLFKQLLMVGGLDKYYQIAKCFRDEDLRADRQPEFTQLDI
EMSFISEEEIISFNESLIKKIWKEVLNIKFNNAFPRMSWQEAMDNYGTDRPDTRYQMLLKDLGGILGDIGFNIFTKAIKS
GGYIKSITVKGGNSSISNVRIKPGGDIFQVAKDAGAGGLAFIRVKGDELETIGAIKNNLSEEHIADILKITEAKDGDLIL
LGAGDKLIVNQSLDRVRQYIAKDLNLIDKSKWNFLWVTDFPMFERNEEENRYEALHHPFCSPKNIKSKDSENLKKEIESS
TANAYDLVLNGLELGGGSLRIHEANLQREVLKMVGLTDKEIDEKFGFLIEALEMGAPPHGGIAFGLDRITMLIIGTDSIR
ETIAFPKNQQAKCLLTNAPSNVSESQLKELDIEITIDE
>Mature_598_residues
MRNKICKELNNTDIGKLVNLCGWVDRRRDHGGVIFIDLRDHSGFLQITINPDDGAALFKQAETLRNETVIMVSGIINERP
KDSINTNLSTGELELKVKDLQILNQIKKNLPFPVSIHDYENTKEELRLKYRYLDLRRGKLLENLKTRHKIIKVAREFLDN
FGFTEVETPLLTKSTPEGARDFLVPARLSNGEFFALPQSPQLFKQLLMVGGLDKYYQIAKCFRDEDLRADRQPEFTQLDI
EMSFISEEEIISFNESLIKKIWKEVLNIKFNNAFPRMSWQEAMDNYGTDRPDTRYQMLLKDLGGILGDIGFNIFTKAIKS
GGYIKSITVKGGNSSISNVRIKPGGDIFQVAKDAGAGGLAFIRVKGDELETIGAIKNNLSEEHIADILKITEAKDGDLIL
LGAGDKLIVNQSLDRVRQYIAKDLNLIDKSKWNFLWVTDFPMFERNEEENRYEALHHPFCSPKNIKSKDSENLKKEIESS
TANAYDLVLNGLELGGGSLRIHEANLQREVLKMVGLTDKEIDEKFGFLIEALEMGAPPHGGIAFGLDRITMLIIGTDSIR
ETIAFPKNQQAKCLLTNAPSNVSESQLKELDIEITIDE

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI40789249, Length=607, Percent_Identity=39.0444810543657, Blast_Score=411, Evalue=1e-115,
Organism=Homo sapiens, GI5031815, Length=265, Percent_Identity=27.9245283018868, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI194272210, Length=265, Percent_Identity=27.9245283018868, Blast_Score=84, Evalue=4e-16,
Organism=Homo sapiens, GI45439306, Length=239, Percent_Identity=26.7782426778243, Blast_Score=83, Evalue=8e-16,
Organism=Escherichia coli, GI1788173, Length=599, Percent_Identity=47.7462437395659, Blast_Score=560, Evalue=1e-160,
Organism=Escherichia coli, GI1789256, Length=299, Percent_Identity=27.0903010033445, Blast_Score=102, Evalue=6e-23,
Organism=Escherichia coli, GI1790571, Length=299, Percent_Identity=27.0903010033445, Blast_Score=101, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI32566633, Length=605, Percent_Identity=35.702479338843, Blast_Score=330, Evalue=2e-90,
Organism=Caenorhabditis elegans, GI71994340, Length=263, Percent_Identity=26.9961977186312, Blast_Score=93, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17535925, Length=263, Percent_Identity=26.9961977186312, Blast_Score=93, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17535927, Length=263, Percent_Identity=26.9961977186312, Blast_Score=93, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17551876, Length=311, Percent_Identity=24.1157556270096, Blast_Score=77, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6325153, Length=659, Percent_Identity=34.1426403641882, Blast_Score=294, Evalue=2e-80,
Organism=Saccharomyces cerevisiae, GI6321807, Length=257, Percent_Identity=28.7937743190661, Blast_Score=87, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6323011, Length=250, Percent_Identity=26.4, Blast_Score=76, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6320242, Length=258, Percent_Identity=26.3565891472868, Blast_Score=76, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6324256, Length=151, Percent_Identity=31.1258278145695, Blast_Score=74, Evalue=7e-14,
Organism=Drosophila melanogaster, GI24584738, Length=606, Percent_Identity=37.4587458745875, Blast_Score=386, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24640849, Length=255, Percent_Identity=28.6274509803922, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24640851, Length=255, Percent_Identity=28.6274509803922, Blast_Score=87, Evalue=4e-17,
Organism=Drosophila melanogaster, GI17136276, Length=248, Percent_Identity=29.4354838709677, Blast_Score=86, Evalue=9e-17,
Organism=Drosophila melanogaster, GI19921528, Length=282, Percent_Identity=25.531914893617, Blast_Score=65, Evalue=9e-11,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYD_PROM0 (A3PFH7)

Other databases:

- EMBL:   CP000576
- RefSeq:   YP_001092103.1
- ProteinModelPortal:   A3PFH7
- SMR:   A3PFH7
- STRING:   A3PFH7
- GeneID:   4912739
- GenomeReviews:   CP000576_GR
- KEGG:   pmg:P9301_18791
- eggNOG:   COG0173
- HOGENOM:   HBG396032
- OMA:   AFPKTQQ
- ProtClustDB:   PRK00476
- BioCyc:   PMAR167546:P9301ORF_1921-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00044_B
- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365
- Gene3D:   G3DSA:3.30.1360.30
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR22594
- PANTHER:   PTHR22594:SF5
- PRINTS:   PR01042
- TIGRFAMs:   TIGR00459

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti; SSF50249 Nucleic_acid_OB

EC number: =6.1.1.12

Molecular weight: Translated: 67656; Mature: 67656

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNKICKELNNTDIGKLVNLCGWVDRRRDHGGVIFIDLRDHSGFLQITINPDDGAALFKQ
CCCHHHHHCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEECCCCEEEEEECCCCCHHHHHH
AETLRNETVIMVSGIINERPKDSINTNLSTGELELKVKDLQILNQIKKNLPFPVSIHDYE
HHHHCCCEEEEEECCCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHCCCCCEEECCCC
NTKEELRLKYRYLDLRRGKLLENLKTRHKIIKVAREFLDNFGFTEVETPLLTKSTPEGAR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCH
DFLVPARLSNGEFFALPQSPQLFKQLLMVGGLDKYYQIAKCFRDEDLRADRQPEFTQLDI
HCEEEEEECCCCEEEECCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCEEEEE
EMSFISEEEIISFNESLIKKIWKEVLNIKFNNAFPRMSWQEAMDNYGTDRPDTRYQMLLK
EEEECCCHHHHHHHHHHHHHHHHHHHCEEECCCCCCCCHHHHHHHCCCCCCCHHHHHHHH
DLGGILGDIGFNIFTKAIKSGGYIKSITVKGGNSSISNVRIKPGGDIFQVAKDAGAGGLA
HHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCEEEECCCCCHHHHHHHCCCCCEE
FIRVKGDELETIGAIKNNLSEEHIADILKITEAKDGDLILLGAGDKLIVNQSLDRVRQYI
EEEEECCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCCEEEEECCCEEEEHHHHHHHHHHH
AKDLNLIDKSKWNFLWVTDFPMFERNEEENRYEALHHPFCSPKNIKSKDSENLKKEIESS
HHHHHHHCCCCCCEEEEECCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHC
TANAYDLVLNGLELGGGSLRIHEANLQREVLKMVGLTDKEIDEKFGFLIEALEMGAPPHG
CCCHHHHHEEEEEECCCEEEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCC
GIAFGLDRITMLIIGTDSIRETIAFPKNQQAKCLLTNAPSNVSESQLKELDIEITIDE
CEEECCCEEEEEEEECHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHCCEEEEECC
>Mature Secondary Structure
MRNKICKELNNTDIGKLVNLCGWVDRRRDHGGVIFIDLRDHSGFLQITINPDDGAALFKQ
CCCHHHHHCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEECCCCEEEEEECCCCCHHHHHH
AETLRNETVIMVSGIINERPKDSINTNLSTGELELKVKDLQILNQIKKNLPFPVSIHDYE
HHHHCCCEEEEEECCCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHCCCCCEEECCCC
NTKEELRLKYRYLDLRRGKLLENLKTRHKIIKVAREFLDNFGFTEVETPLLTKSTPEGAR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCH
DFLVPARLSNGEFFALPQSPQLFKQLLMVGGLDKYYQIAKCFRDEDLRADRQPEFTQLDI
HCEEEEEECCCCEEEECCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCEEEEE
EMSFISEEEIISFNESLIKKIWKEVLNIKFNNAFPRMSWQEAMDNYGTDRPDTRYQMLLK
EEEECCCHHHHHHHHHHHHHHHHHHHCEEECCCCCCCCHHHHHHHCCCCCCCHHHHHHHH
DLGGILGDIGFNIFTKAIKSGGYIKSITVKGGNSSISNVRIKPGGDIFQVAKDAGAGGLA
HHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCEEEECCCCCHHHHHHHCCCCCEE
FIRVKGDELETIGAIKNNLSEEHIADILKITEAKDGDLILLGAGDKLIVNQSLDRVRQYI
EEEEECCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCCEEEEECCCEEEEHHHHHHHHHHH
AKDLNLIDKSKWNFLWVTDFPMFERNEEENRYEALHHPFCSPKNIKSKDSENLKKEIESS
HHHHHHHCCCCCCEEEEECCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHC
TANAYDLVLNGLELGGGSLRIHEANLQREVLKMVGLTDKEIDEKFGFLIEALEMGAPPHG
CCCHHHHHEEEEEECCCEEEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCC
GIAFGLDRITMLIIGTDSIRETIAFPKNQQAKCLLTNAPSNVSESQLKELDIEITIDE
CEEECCCEEEEEEEECHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA