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The map label for this gene is pyrG

Identifier: 126697218

GI number: 126697218

Start: 1607101

End: 1608711

Strand: Direct

Name: pyrG

Synonym: P9301_18801

Alternate gene names: 126697218

Gene position: 1607101-1608711 (Clockwise)

Preceding gene: 126697217

Following gene: 126697219

Centisome position: 97.88

GC content: 34.2

Gene sequence:

>1611_bases
ATGTCAAAATTTGTATTTGTCACCGGAGGAGTTGTTTCTAGCATTGGAAAAGGAATTGTAGCTGCAAGCTTAGGAAGATT
ATTAAAGTCCAGAGGATATAGTGTTTCAATATTAAAACTAGATCCATATCTAAATGTTGATCCAGGCACAATGAGTCCTT
TCCAACATGGAGAAGTATTTGTAACCGAAGATGGGGCTGAAACCGATCTAGATTTAGGTCACTATGAAAGATTTACTGAT
ACTGCAATGACTAGGTTAAATAGTGTGACTACGGGATCTATCTATCAAGCAGTTATCAATAAAGAAAGAAGAGGTAGTTA
TAACGGTGGAACTGTGCAAGTAATACCTCATATAACAGGAGAAATAAGAGAAAGAATTCATAGAGTAGCCGCCAACAGTA
ATGCAGATATTATTATTACTGAAATTGGTGGAACAGTTGGTGATATTGAATCTCTACCTTTTTTAGAGGCAATAAGAGAA
TTCAAAAATGATGTCAATAGGAACGATGTTGCATACATTCACGTAACATTACTTCCTTACATCAAAACCTCTGGCGAAAT
AAAAACTAAACCAACACAGCATTCAGTTAAAGAATTAAGATCAATTGGAATTCAGCCAGATTTACTTGTATGCCGAAGTG
ATAAATCTATCAATGAAGCTCTAAAAAAGAAGCTTAGTGGTTTTTGCGGTGTCAGTATTAACTCTGTAATTGAAGCTTTA
GACGCAGACAGTATTTATTCGGTACCTCTTTCTTTAAAAAAAGAAGGTTTATGCAAAGAAACCTTGAATTATCTAGAACT
TGAAGATAAAAAATGTGATTTGAAAAATTGGGAGCAACTAATACACAACCTAAGAAATCCTGGAGGTCCAATCAAAGTTG
CTTTAGTAGGTAAATATATTGAACTTGGAGATGCATATTTATCTGTAGTTGAAGCTTTAAGACATGCATGCATTGAACAA
AAAGCTTTATTAGATTTACATTGGGTGAGTGCTGAAATGATAGAAAAAAATTCAGCAGAAACTTACTTAAATGAAGTTGA
TGCAATTGTCGTACCCGGAGGATTTGGCAATAGAGGAGTCAATGGAAAAATTGCGGCTATAAAATTCGCAAGAGAAAATA
AAATTCCCTTTTTAGGTTTGTGCCTTGGTATGCAATGTGCAGTTATTGAATGGGCCAGGAATGTAGCTCATCTTCCAGAT
GCATCTAGTTCAGAACTAGACCCAAACACTCCCAATCCAGTGATACATTTATTACCAGAACAGCAAGATGTAGTTGATTT
AGGTGGAACAATGAGACTTGGAGTATATCCATGTAGATTGACAAAAAATACAACTGGAAAAAACTTATATGATGAGGATG
TTATTTATGAGAGACATCGACATAGATACGAATTTAATAATTACTATAAACAAAGTTTTTTAGATTCTGGATACAAAATT
AGTGGTACATCACCAGATGGCAGATTAGTTGAGTTAATTGAGTTGGAAAATCATCCTTACTTCTTAGCATGTCAATATCA
TCCCGAGTTTTTATCAAGACCTGGCAAACCTCATCCTTTATTTAAAGGTTTAATAAAAGCCTCTCAAGATAAGTTAACTC
AATCAAATTAA

Upstream 100 bases:

>100_bases
TATTGAAATAACAATTGATGAATAAGAATATATATGGATGTACTAAAAGTTTTTTGTTTAAATAAAATATAAGGAGGCTG
TGCTTAATTAAAAATATTTA

Downstream 100 bases:

>100_bases
TATTCTTTATTTTTTTGAATGAAAAAATGACAAATTTTTTACCATTAGTCGAACAATTTCATTCATTACAAGGTGAAGGT
TATCACGCTGGAAAAAGTGC

Product: CTP synthetase

Products: NA

Alternate protein names: CTP synthetase; UTP--ammonia ligase

Number of amino acids: Translated: 536; Mature: 535

Protein sequence:

>536_residues
MSKFVFVTGGVVSSIGKGIVAASLGRLLKSRGYSVSILKLDPYLNVDPGTMSPFQHGEVFVTEDGAETDLDLGHYERFTD
TAMTRLNSVTTGSIYQAVINKERRGSYNGGTVQVIPHITGEIRERIHRVAANSNADIIITEIGGTVGDIESLPFLEAIRE
FKNDVNRNDVAYIHVTLLPYIKTSGEIKTKPTQHSVKELRSIGIQPDLLVCRSDKSINEALKKKLSGFCGVSINSVIEAL
DADSIYSVPLSLKKEGLCKETLNYLELEDKKCDLKNWEQLIHNLRNPGGPIKVALVGKYIELGDAYLSVVEALRHACIEQ
KALLDLHWVSAEMIEKNSAETYLNEVDAIVVPGGFGNRGVNGKIAAIKFARENKIPFLGLCLGMQCAVIEWARNVAHLPD
ASSSELDPNTPNPVIHLLPEQQDVVDLGGTMRLGVYPCRLTKNTTGKNLYDEDVIYERHRHRYEFNNYYKQSFLDSGYKI
SGTSPDGRLVELIELENHPYFLACQYHPEFLSRPGKPHPLFKGLIKASQDKLTQSN

Sequences:

>Translated_536_residues
MSKFVFVTGGVVSSIGKGIVAASLGRLLKSRGYSVSILKLDPYLNVDPGTMSPFQHGEVFVTEDGAETDLDLGHYERFTD
TAMTRLNSVTTGSIYQAVINKERRGSYNGGTVQVIPHITGEIRERIHRVAANSNADIIITEIGGTVGDIESLPFLEAIRE
FKNDVNRNDVAYIHVTLLPYIKTSGEIKTKPTQHSVKELRSIGIQPDLLVCRSDKSINEALKKKLSGFCGVSINSVIEAL
DADSIYSVPLSLKKEGLCKETLNYLELEDKKCDLKNWEQLIHNLRNPGGPIKVALVGKYIELGDAYLSVVEALRHACIEQ
KALLDLHWVSAEMIEKNSAETYLNEVDAIVVPGGFGNRGVNGKIAAIKFARENKIPFLGLCLGMQCAVIEWARNVAHLPD
ASSSELDPNTPNPVIHLLPEQQDVVDLGGTMRLGVYPCRLTKNTTGKNLYDEDVIYERHRHRYEFNNYYKQSFLDSGYKI
SGTSPDGRLVELIELENHPYFLACQYHPEFLSRPGKPHPLFKGLIKASQDKLTQSN
>Mature_535_residues
SKFVFVTGGVVSSIGKGIVAASLGRLLKSRGYSVSILKLDPYLNVDPGTMSPFQHGEVFVTEDGAETDLDLGHYERFTDT
AMTRLNSVTTGSIYQAVINKERRGSYNGGTVQVIPHITGEIRERIHRVAANSNADIIITEIGGTVGDIESLPFLEAIREF
KNDVNRNDVAYIHVTLLPYIKTSGEIKTKPTQHSVKELRSIGIQPDLLVCRSDKSINEALKKKLSGFCGVSINSVIEALD
ADSIYSVPLSLKKEGLCKETLNYLELEDKKCDLKNWEQLIHNLRNPGGPIKVALVGKYIELGDAYLSVVEALRHACIEQK
ALLDLHWVSAEMIEKNSAETYLNEVDAIVVPGGFGNRGVNGKIAAIKFARENKIPFLGLCLGMQCAVIEWARNVAHLPDA
SSSELDPNTPNPVIHLLPEQQDVVDLGGTMRLGVYPCRLTKNTTGKNLYDEDVIYERHRHRYEFNNYYKQSFLDSGYKIS
GTSPDGRLVELIELENHPYFLACQYHPEFLSRPGKPHPLFKGLIKASQDKLTQSN

Specific function: Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen

COG id: COG0504

COG function: function code F; CTP synthase (UTP-ammonia lyase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Homo sapiens, GI148491070, Length=553, Percent_Identity=45.9312839059675, Blast_Score=488, Evalue=1e-138,
Organism=Homo sapiens, GI28559085, Length=552, Percent_Identity=46.3768115942029, Blast_Score=472, Evalue=1e-133,
Organism=Homo sapiens, GI28559083, Length=552, Percent_Identity=46.3768115942029, Blast_Score=472, Evalue=1e-133,
Organism=Homo sapiens, GI221316689, Length=552, Percent_Identity=46.3768115942029, Blast_Score=472, Evalue=1e-133,
Organism=Escherichia coli, GI1789142, Length=539, Percent_Identity=52.3191094619666, Blast_Score=568, Evalue=1e-163,
Organism=Caenorhabditis elegans, GI25148299, Length=612, Percent_Identity=38.7254901960784, Blast_Score=424, Evalue=1e-119,
Organism=Saccharomyces cerevisiae, GI6322563, Length=566, Percent_Identity=43.9929328621908, Blast_Score=459, Evalue=1e-130,
Organism=Saccharomyces cerevisiae, GI6319432, Length=558, Percent_Identity=42.6523297491039, Blast_Score=444, Evalue=1e-125,
Organism=Drosophila melanogaster, GI24664469, Length=557, Percent_Identity=47.0377019748653, Blast_Score=493, Evalue=1e-139,
Organism=Drosophila melanogaster, GI21357815, Length=502, Percent_Identity=46.0159362549801, Blast_Score=420, Evalue=1e-117,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PYRG_PROM0 (A3PFH8)

Other databases:

- EMBL:   CP000576
- RefSeq:   YP_001092104.1
- ProteinModelPortal:   A3PFH8
- SMR:   A3PFH8
- STRING:   A3PFH8
- GeneID:   4912740
- GenomeReviews:   CP000576_GR
- KEGG:   pmg:P9301_18801
- eggNOG:   COG0504
- HOGENOM:   HBG597806
- OMA:   RVTMQKL
- ProtClustDB:   PRK05380
- BioCyc:   PMAR167546:P9301ORF_1922-MONOMER
- HAMAP:   MF_01227
- InterPro:   IPR004468
- InterPro:   IPR017456
- InterPro:   IPR017926
- InterPro:   IPR000991
- TIGRFAMs:   TIGR00337

Pfam domain/function: PF06418 CTP_synth_N; PF00117 GATase

EC number: =6.3.4.2

Molecular weight: Translated: 59471; Mature: 59339

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 381-381 ACT_SITE 507-507 ACT_SITE 509-509

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKFVFVTGGVVSSIGKGIVAASLGRLLKSRGYSVSILKLDPYLNVDPGTMSPFQHGEVF
CCCEEEEECHHHHHHCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCEEE
VTEDGAETDLDLGHYERFTDTAMTRLNSVTTGSIYQAVINKERRGSYNGGTVQVIPHITG
EECCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCEEEEECCCHH
EIRERIHRVAANSNADIIITEIGGTVGDIESLPFLEAIREFKNDVNRNDVAYIHVTLLPY
HHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEEEEE
IKTSGEIKTKPTQHSVKELRSIGIQPDLLVCRSDKSINEALKKKLSGFCGVSINSVIEAL
ECCCCCEECCCCHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHCCCCCCCHHHHHHHH
DADSIYSVPLSLKKEGLCKETLNYLELEDKKCDLKNWEQLIHNLRNPGGPIKVALVGKYI
CCCCEEECCCHHCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEEECHH
ELGDAYLSVVEALRHACIEQKALLDLHWVSAEMIEKNSAETYLNEVDAIVVPGGFGNRGV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCEEEECCCCCCCCC
NGKIAAIKFARENKIPFLGLCLGMQCAVIEWARNVAHLPDASSSELDPNTPNPVIHLLPE
CCEEEEEEEECCCCCCEEEHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCC
QQDVVDLGGTMRLGVYPCRLTKNTTGKNLYDEDVIYERHRHRYEFNNYYKQSFLDSGYKI
CCCHHHCCCEEEEEEEEEEEECCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCEE
SGTSPDGRLVELIELENHPYFLACQYHPEFLSRPGKPHPLFKGLIKASQDKLTQSN
ECCCCCCCEEEEEEECCCCEEEEEEECHHHHCCCCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SKFVFVTGGVVSSIGKGIVAASLGRLLKSRGYSVSILKLDPYLNVDPGTMSPFQHGEVF
CCEEEEECHHHHHHCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCEEE
VTEDGAETDLDLGHYERFTDTAMTRLNSVTTGSIYQAVINKERRGSYNGGTVQVIPHITG
EECCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCEEEEECCCHH
EIRERIHRVAANSNADIIITEIGGTVGDIESLPFLEAIREFKNDVNRNDVAYIHVTLLPY
HHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEEEEE
IKTSGEIKTKPTQHSVKELRSIGIQPDLLVCRSDKSINEALKKKLSGFCGVSINSVIEAL
ECCCCCEECCCCHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHCCCCCCCHHHHHHHH
DADSIYSVPLSLKKEGLCKETLNYLELEDKKCDLKNWEQLIHNLRNPGGPIKVALVGKYI
CCCCEEECCCHHCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEEECHH
ELGDAYLSVVEALRHACIEQKALLDLHWVSAEMIEKNSAETYLNEVDAIVVPGGFGNRGV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCEEEECCCCCCCCC
NGKIAAIKFARENKIPFLGLCLGMQCAVIEWARNVAHLPDASSSELDPNTPNPVIHLLPE
CCEEEEEEEECCCCCCEEEHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCC
QQDVVDLGGTMRLGVYPCRLTKNTTGKNLYDEDVIYERHRHRYEFNNYYKQSFLDSGYKI
CCCHHHCCCEEEEEEEEEEEECCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCEE
SGTSPDGRLVELIELENHPYFLACQYHPEFLSRPGKPHPLFKGLIKASQDKLTQSN
ECCCCCCCEEEEEEECCCCEEEEEEECHHHHCCCCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA