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The map label for this gene is speB [C]

Identifier: 126697215

GI number: 126697215

Start: 1603129

End: 1604010

Strand: Direct

Name: speB [C]

Synonym: P9301_18771

Alternate gene names: 126697215

Gene position: 1603129-1604010 (Clockwise)

Preceding gene: 126697214

Following gene: 126697216

Centisome position: 97.64

GC content: 32.65

Gene sequence:

>882_bases
ATGACAAAAAATTTATTTAATAACGAAAATGCAATTTATATGGGATCAAAAAGAAATCCCAAGAATTGCTCAATTGGTAT
ATTTGGAGTTAATTACGACGGGACATGTTCGTTTAAACCAGGAGCAAGATTTGGTCCAGAAGCGATAAGACAAGTCAGTT
CTTGTTTAGAAACATACTGTCCAAAACTAAACAAAGATTTAGAAGATATTATGTATGTTGATTTTGGATCAATACTAATT
GATAAAAATGACTCAAAGTCCGTTATTGAATCAGTTAAATCTGCAACAAATTATTTAATTAGTAGACGCCTTAGTCCTAT
TATGCTTGGAGGCGAACACTCTATTACAAGAGGTGCTATTGAAGCTTTAGTAAAAAAATATCCAGATTTGATATTGGTTC
AACTTGATGCTCATGCAGATTTAAGAGAATCATATATAGGAAATGAACATAGTCATGCTTGTACTATGAAAAGATGCTTA
GAAGTACTACCTGAAAAGAAAATTTTGCAAGTAGGAATTAGAAGTGGGACTAAGGAAGAATTTGAAATTATGCATAACAA
CAACCAATTAGTTAACTTTTGTCCAGGAGGAAATGTACATGAGTTAAAACAAGCTCTTCTACCATACTCTAAGTCTCCCA
TCTATTTAACAATAGATTTAGATTGGTTTGATCCCAGTTTATTAGCAGGGACGGGCACTCCAGAACCGGGAGGATTTTTT
TGGAATGATTTTGAAGAAATACTGAAAACTCTAAAAGGTTTTAGAATTGTGGCTTCAGACATTGTGGAATTATCTCCAGA
AATTGATAAAAGCGGAGTAAGTAGCATTGTTGCAGCCAAAGTACTTAGAAGCTTAATTTTGTCATTAGAAAATATGCAAT
AA

Upstream 100 bases:

>100_bases
CTAAAAATTGAAAAAGGATGTGAAATTTGGAATTTAAATTTTCAAAATGCAGCATTCAAAATGATGCCAAATAAAATTAT
AAAAGAACTAGATTCATAAG

Downstream 100 bases:

>100_bases
AAAATTTCTAAACTACAGTGGAAAAATACTAAATACAAACTAAATATTTCATGGATTTGCGAAAAAGTCCTCTTTATTCA
AAATATATTGAATCCAATGC

Product: arginase

Products: putrescine; urea

Alternate protein names: NA

Number of amino acids: Translated: 293; Mature: 292

Protein sequence:

>293_residues
MTKNLFNNENAIYMGSKRNPKNCSIGIFGVNYDGTCSFKPGARFGPEAIRQVSSCLETYCPKLNKDLEDIMYVDFGSILI
DKNDSKSVIESVKSATNYLISRRLSPIMLGGEHSITRGAIEALVKKYPDLILVQLDAHADLRESYIGNEHSHACTMKRCL
EVLPEKKILQVGIRSGTKEEFEIMHNNNQLVNFCPGGNVHELKQALLPYSKSPIYLTIDLDWFDPSLLAGTGTPEPGGFF
WNDFEEILKTLKGFRIVASDIVELSPEIDKSGVSSIVAAKVLRSLILSLENMQ

Sequences:

>Translated_293_residues
MTKNLFNNENAIYMGSKRNPKNCSIGIFGVNYDGTCSFKPGARFGPEAIRQVSSCLETYCPKLNKDLEDIMYVDFGSILI
DKNDSKSVIESVKSATNYLISRRLSPIMLGGEHSITRGAIEALVKKYPDLILVQLDAHADLRESYIGNEHSHACTMKRCL
EVLPEKKILQVGIRSGTKEEFEIMHNNNQLVNFCPGGNVHELKQALLPYSKSPIYLTIDLDWFDPSLLAGTGTPEPGGFF
WNDFEEILKTLKGFRIVASDIVELSPEIDKSGVSSIVAAKVLRSLILSLENMQ
>Mature_292_residues
TKNLFNNENAIYMGSKRNPKNCSIGIFGVNYDGTCSFKPGARFGPEAIRQVSSCLETYCPKLNKDLEDIMYVDFGSILID
KNDSKSVIESVKSATNYLISRRLSPIMLGGEHSITRGAIEALVKKYPDLILVQLDAHADLRESYIGNEHSHACTMKRCLE
VLPEKKILQVGIRSGTKEEFEIMHNNNQLVNFCPGGNVHELKQALLPYSKSPIYLTIDLDWFDPSLLAGTGTPEPGGFFW
NDFEEILKTLKGFRIVASDIVELSPEIDKSGVSSIVAAKVLRSLILSLENMQ

Specific function: Catalyzes The Formation Of Putrescine From Agmatine. [C]

COG id: COG0010

COG function: function code E; Arginase/agmatinase/formimionoglutamate hydrolase, arginase family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the arginase family [H]

Homologues:

Organism=Homo sapiens, GI37537722, Length=290, Percent_Identity=27.9310344827586, Blast_Score=138, Evalue=5e-33,
Organism=Escherichia coli, GI1789306, Length=256, Percent_Identity=29.296875, Blast_Score=100, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6325146, Length=230, Percent_Identity=26.9565217391304, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005925
- InterPro:   IPR006035
- InterPro:   IPR020855 [H]

Pfam domain/function: PF00491 Arginase [H]

EC number: 3.5.3.11

Molecular weight: Translated: 32611; Mature: 32480

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS00012 PHOSPHOPANTETHEINE ; PS00147 ARGINASE_1 ; PS00148 ARGINASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKNLFNNENAIYMGSKRNPKNCSIGIFGVNYDGTCSFKPGARFGPEAIRQVSSCLETYC
CCCCCCCCCCEEEECCCCCCCCCEEEEEEECCCCEECCCCCCCCCHHHHHHHHHHHHHHC
PKLNKDLEDIMYVDFGSILIDKNDSKSVIESVKSATNYLISRRLSPIMLGGEHSITRGAI
HHCCCCHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHH
EALVKKYPDLILVQLDAHADLRESYIGNEHSHACTMKRCLEVLPEKKILQVGIRSGTKEE
HHHHHHCCCEEEEEECCCCHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCHHH
FEIMHNNNQLVNFCPGGNVHELKQALLPYSKSPIYLTIDLDWFDPSLLAGTGTPEPGGFF
HHEEECCCCEEEECCCCCHHHHHHHHCCCCCCCEEEEEEECCCCCCCEECCCCCCCCCCC
WNDFEEILKTLKGFRIVASDIVELSPEIDKSGVSSIVAAKVLRSLILSLENMQ
HHHHHHHHHHHHCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TKNLFNNENAIYMGSKRNPKNCSIGIFGVNYDGTCSFKPGARFGPEAIRQVSSCLETYC
CCCCCCCCCEEEECCCCCCCCCEEEEEEECCCCEECCCCCCCCCHHHHHHHHHHHHHHC
PKLNKDLEDIMYVDFGSILIDKNDSKSVIESVKSATNYLISRRLSPIMLGGEHSITRGAI
HHCCCCHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHH
EALVKKYPDLILVQLDAHADLRESYIGNEHSHACTMKRCLEVLPEKKILQVGIRSGTKEE
HHHHHHCCCEEEEEECCCCHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCHHH
FEIMHNNNQLVNFCPGGNVHELKQALLPYSKSPIYLTIDLDWFDPSLLAGTGTPEPGGFF
HHEEECCCCEEEECCCCCHHHHHHHHCCCCCCCEEEEEEECCCCCCCEECCCCCCCCCCC
WNDFEEILKTLKGFRIVASDIVELSPEIDKSGVSSIVAAKVLRSLILSLENMQ
HHHHHHHHHHHHCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: agmatine; H2O

Specific reaction: agmatine + H2O = putrescine + urea

General reaction: Amidine hydrolysis [C]

Inhibitor: EDTA; EGTA [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]