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The map label for this gene is speE

Identifier: 126697214

GI number: 126697214

Start: 1602276

End: 1603127

Strand: Direct

Name: speE

Synonym: P9301_18761

Alternate gene names: 126697214

Gene position: 1602276-1603127 (Clockwise)

Preceding gene: 126697213

Following gene: 126697215

Centisome position: 97.59

GC content: 28.64

Gene sequence:

>852_bases
ATGACTAATATTCAAACATGGATAGATGAATATCATAAAGGCTCAAGATTCGGTCTAAATGGAGATGTTTTAATTAAACA
AAAATCACAATATCAAGAAATTATTGTTATTGAAAATGAATACTATGGCAGAGCTTTAATGCTTGATGGTTGTTGGATGA
CATCACTAAAAGACGAAAAATATTATCATGAGTGTCTTGTGCATCCAGCATTAAGTAGCATTGACGAAAAATCTAATGTA
CTAATTATTGGTGGAGGAGATGGCGGTACAGTAAGAGAATGCGTTAAATATGCTCAAATATCAAAAATTGATCTAGTAGA
AATTGATGAGGAGGTAATCAAAATATCTAAAAAATTTTTAAAAGAAATAGGAGGCGAAGCATGGCATGACAAAAGATTAG
AAATACATGTTGATGATGGTGTTAAATGGGTAAAAAAAACAAGAGATAATTATTACGACGTTATTTTTATAGATTGTTCA
GATCCCTCTGAATTTTCAAATTTATTATTTTCAGATTCTTTTTATAAAGAATGTAAAAGAATACTTACACCAAAAGGGAT
ATTAGCAACGCAAAGCGAATCTCCTGAATCCTTCAAAAATATTCACATAAATATTTTGAAAACTTTAAAAAATATATTTA
AAGTTTCTGAAACTATGTATTCCTTTGTGCCTATATATCCAAGCGGCATTTGGAGTTGGACATTCGCTTCTTCAGAGGAT
CTAAATTTATCAAAGCAAAATTATGATGAAATCCTAAAAATTGAAAAAGGATGTGAAATTTGGAATTTAAATTTTCAAAA
TGCAGCATTCAAAATGATGCCAAATAAAATTATAAAAGAACTAGATTCATAA

Upstream 100 bases:

>100_bases
ATCATGCAGGCGATAATATTAAAAAACAAACTCCTAAAGAATTTCAACGGCTTTGGCAAATAGCCAAGCGAAAACTTAAA
GGAAAAATTCTTAAAAGCAA

Downstream 100 bases:

>100_bases
GATGACAAAAAATTTATTTAATAACGAAAATGCAATTTATATGGGATCAAAAAGAAATCCCAAGAATTGCTCAATTGGTA
TATTTGGAGTTAATTACGAC

Product: spermidine synthase

Products: NA

Alternate protein names: Putrescine aminopropyltransferase; PAPT; SPDSY

Number of amino acids: Translated: 283; Mature: 282

Protein sequence:

>283_residues
MTNIQTWIDEYHKGSRFGLNGDVLIKQKSQYQEIIVIENEYYGRALMLDGCWMTSLKDEKYYHECLVHPALSSIDEKSNV
LIIGGGDGGTVRECVKYAQISKIDLVEIDEEVIKISKKFLKEIGGEAWHDKRLEIHVDDGVKWVKKTRDNYYDVIFIDCS
DPSEFSNLLFSDSFYKECKRILTPKGILATQSESPESFKNIHINILKTLKNIFKVSETMYSFVPIYPSGIWSWTFASSED
LNLSKQNYDEILKIEKGCEIWNLNFQNAAFKMMPNKIIKELDS

Sequences:

>Translated_283_residues
MTNIQTWIDEYHKGSRFGLNGDVLIKQKSQYQEIIVIENEYYGRALMLDGCWMTSLKDEKYYHECLVHPALSSIDEKSNV
LIIGGGDGGTVRECVKYAQISKIDLVEIDEEVIKISKKFLKEIGGEAWHDKRLEIHVDDGVKWVKKTRDNYYDVIFIDCS
DPSEFSNLLFSDSFYKECKRILTPKGILATQSESPESFKNIHINILKTLKNIFKVSETMYSFVPIYPSGIWSWTFASSED
LNLSKQNYDEILKIEKGCEIWNLNFQNAAFKMMPNKIIKELDS
>Mature_282_residues
TNIQTWIDEYHKGSRFGLNGDVLIKQKSQYQEIIVIENEYYGRALMLDGCWMTSLKDEKYYHECLVHPALSSIDEKSNVL
IIGGGDGGTVRECVKYAQISKIDLVEIDEEVIKISKKFLKEIGGEAWHDKRLEIHVDDGVKWVKKTRDNYYDVIFIDCSD
PSEFSNLLFSDSFYKECKRILTPKGILATQSESPESFKNIHINILKTLKNIFKVSETMYSFVPIYPSGIWSWTFASSEDL
NLSKQNYDEILKIEKGCEIWNLNFQNAAFKMMPNKIIKELDS

Specific function: Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM), which serves as an aminopropyl donor

COG id: COG0421

COG function: function code E; Spermidine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the spermidine/spermine synthase family

Homologues:

Organism=Homo sapiens, GI63253298, Length=267, Percent_Identity=32.5842696629214, Blast_Score=138, Evalue=5e-33,
Organism=Escherichia coli, GI1786312, Length=279, Percent_Identity=31.8996415770609, Blast_Score=149, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI212645616, Length=230, Percent_Identity=34.7826086956522, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI25154106, Length=256, Percent_Identity=33.984375, Blast_Score=110, Evalue=8e-25,
Organism=Saccharomyces cerevisiae, GI6325326, Length=267, Percent_Identity=34.4569288389513, Blast_Score=145, Evalue=9e-36,
Organism=Saccharomyces cerevisiae, GI6323175, Length=288, Percent_Identity=32.2916666666667, Blast_Score=138, Evalue=1e-33,
Organism=Drosophila melanogaster, GI24645443, Length=263, Percent_Identity=31.5589353612167, Blast_Score=122, Evalue=3e-28,
Organism=Drosophila melanogaster, GI21355941, Length=196, Percent_Identity=30.6122448979592, Blast_Score=87, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SPEE_PROM0 (A3PFH4)

Other databases:

- EMBL:   CP000576
- RefSeq:   YP_001092100.1
- ProteinModelPortal:   A3PFH4
- SMR:   A3PFH4
- STRING:   A3PFH4
- GeneID:   4912736
- GenomeReviews:   CP000576_GR
- KEGG:   pmg:P9301_18761
- eggNOG:   COG0421
- HOGENOM:   HBG635113
- OMA:   WSWTFAS
- ProtClustDB:   PRK00811
- BioCyc:   PMAR167546:P9301ORF_1918-MONOMER
- HAMAP:   MF_00198
- InterPro:   IPR001045
- PANTHER:   PTHR11558

Pfam domain/function: PF01564 Spermine_synth

EC number: =2.5.1.16

Molecular weight: Translated: 32762; Mature: 32631

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: PS01330 SPERMIDINE_SYNTHASE_1; PS51006 SPERMIDINE_SYNTHASE_2

Important sites: BINDING 32-32 BINDING 87-87 BINDING 107-107 BINDING 158-158 BINDING 161-161

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNIQTWIDEYHKGSRFGLNGDVLIKQKSQYQEIIVIENEYYGRALMLDGCWMTSLKDEK
CCCHHHHHHHHCCCCCCCCCCCEEEEECCCCCEEEEEECCCCCCEEEECCCEECCCCCHH
YYHECLVHPALSSIDEKSNVLIIGGGDGGTVRECVKYAQISKIDLVEIDEEVIKISKKFL
HHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHH
KEIGGEAWHDKRLEIHVDDGVKWVKKTRDNYYDVIFIDCSDPSEFSNLLFSDSFYKECKR
HHHCCCCCCCCEEEEEECCCHHHHHHHCCCEEEEEEEECCCHHHHHHHHHCHHHHHHHHH
ILTPKGILATQSESPESFKNIHINILKTLKNIFKVSETMYSFVPIYPSGIWSWTFASSED
HCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEEEEECCCCC
LNLSKQNYDEILKIEKGCEIWNLNFQNAAFKMMPNKIIKELDS
CCCCCCCHHHHHHHHCCCEEEECCCCCHHHHHCHHHHHHHHCC
>Mature Secondary Structure 
TNIQTWIDEYHKGSRFGLNGDVLIKQKSQYQEIIVIENEYYGRALMLDGCWMTSLKDEK
CCHHHHHHHHCCCCCCCCCCCEEEEECCCCCEEEEEECCCCCCEEEECCCEECCCCCHH
YYHECLVHPALSSIDEKSNVLIIGGGDGGTVRECVKYAQISKIDLVEIDEEVIKISKKFL
HHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHH
KEIGGEAWHDKRLEIHVDDGVKWVKKTRDNYYDVIFIDCSDPSEFSNLLFSDSFYKECKR
HHHCCCCCCCCEEEEEECCCHHHHHHHCCCEEEEEEEECCCHHHHHHHHHCHHHHHHHHH
ILTPKGILATQSESPESFKNIHINILKTLKNIFKVSETMYSFVPIYPSGIWSWTFASSED
HCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEEEEECCCCC
LNLSKQNYDEILKIEKGCEIWNLNFQNAAFKMMPNKIIKELDS
CCCCCCCHHHHHHHHCCCEEEECCCCCHHHHHCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA