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The map label for this gene is gcvH

Identifier: 126697197

GI number: 126697197

Start: 1586236

End: 1586625

Strand: Reverse

Name: gcvH

Synonym: P9301_18591

Alternate gene names: 126697197

Gene position: 1586625-1586236 (Counterclockwise)

Preceding gene: 126697198

Following gene: 126697196

Centisome position: 96.63

GC content: 35.9

Gene sequence:

>390_bases
ATGTCTTACAAATTTCCAGACAACCTCAACTATGCTGATACTCATGAATATGTCTTGGAAGAAAATGGATTATTAAAAAT
TGGAGTTAGTGAATTCGCTATAGATCAATTAGGAGATATTGTTTTTGTTGAATTAGCTGATGAAGGTGCGACTTTAGAGA
AAGGCGAGACTTTTGGAACAATAGAATCAGTTAAGGCCGTTGAGGAAGTCTATCTACCTTTTTCTGGGGAAATAGTATCT
GTAAATGAAAGTGTTATTGAGAACCCTGAGCTTTTGCAGAGTGATCCGATTGGAGACGGTTGGTTAGTCATTTTGAAACC
AGAATCAAAAGCATCAATTGCTGATTTGATGACTTCTGAGGAATACCAATCAAAGGTTGTACCAAAATAA

Upstream 100 bases:

>100_bases
ACATAGCTCACATCAAAATTGCATTAATTCGATTATTATCTGAATTACTACAGGAAAAATTAATTTCAAAGGATTCTCTA
CTTCCTTTATCTACTTAATC

Downstream 100 bases:

>100_bases
GACAAACTTTTTAAAAGGAGCTATTTTAAAGAAAAACATTTTAATATGACATCCAAATTTGGGTCTGATTTGTTTATAGA
TAGGCATCTTGGGTTAGGAG

Product: glycine cleavage system protein H

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 129; Mature: 128

Protein sequence:

>129_residues
MSYKFPDNLNYADTHEYVLEENGLLKIGVSEFAIDQLGDIVFVELADEGATLEKGETFGTIESVKAVEEVYLPFSGEIVS
VNESVIENPELLQSDPIGDGWLVILKPESKASIADLMTSEEYQSKVVPK

Sequences:

>Translated_129_residues
MSYKFPDNLNYADTHEYVLEENGLLKIGVSEFAIDQLGDIVFVELADEGATLEKGETFGTIESVKAVEEVYLPFSGEIVS
VNESVIENPELLQSDPIGDGWLVILKPESKASIADLMTSEEYQSKVVPK
>Mature_128_residues
SYKFPDNLNYADTHEYVLEENGLLKIGVSEFAIDQLGDIVFVELADEGATLEKGETFGTIESVKAVEEVYLPFSGEIVSV
NESVIENPELLQSDPIGDGWLVILKPESKASIADLMTSEEYQSKVVPK

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain

Homologues:

Organism=Homo sapiens, GI89057342, Length=113, Percent_Identity=39.8230088495575, Blast_Score=100, Evalue=5e-22,
Organism=Homo sapiens, GI49574537, Length=113, Percent_Identity=39.8230088495575, Blast_Score=99, Evalue=9e-22,
Organism=Escherichia coli, GI1789271, Length=120, Percent_Identity=37.5, Blast_Score=94, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17507493, Length=112, Percent_Identity=41.9642857142857, Blast_Score=100, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17551294, Length=120, Percent_Identity=35.8333333333333, Blast_Score=93, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6319272, Length=130, Percent_Identity=34.6153846153846, Blast_Score=92, Evalue=2e-20,
Organism=Drosophila melanogaster, GI17865652, Length=116, Percent_Identity=35.3448275862069, Blast_Score=75, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GCSH_PROM0 (A3PFF7)

Other databases:

- EMBL:   CP000576
- RefSeq:   YP_001092083.1
- ProteinModelPortal:   A3PFF7
- SMR:   A3PFF7
- STRING:   A3PFF7
- GeneID:   4910950
- GenomeReviews:   CP000576_GR
- KEGG:   pmg:P9301_18591
- eggNOG:   COG0509
- HOGENOM:   HBG693789
- OMA:   NDDPYDG
- ProtClustDB:   PRK01202
- BioCyc:   PMAR167546:P9301ORF_1900-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_00272
- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053
- PANTHER:   PTHR11715
- TIGRFAMs:   TIGR00527

Pfam domain/function: PF01597 GCV_H; SSF51230 Hybrid_motif

EC number: NA

Molecular weight: Translated: 14216; Mature: 14085

Theoretical pI: Translated: 3.79; Mature: 3.79

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYKFPDNLNYADTHEYVLEENGLLKIGVSEFAIDQLGDIVFVELADEGATLEKGETFGT
CCCCCCCCCCCCCCCCEEECCCCEEEECHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCH
IESVKAVEEVYLPFSGEIVSVNESVIENPELLQSDPIGDGWLVILKPESKASIADLMTSE
HHHHHHHHHHCCCCCCCEEECCHHHHCCCCHHCCCCCCCCEEEEECCCCCCHHHHHHHCH
EYQSKVVPK
HHHHCCCCC
>Mature Secondary Structure 
SYKFPDNLNYADTHEYVLEENGLLKIGVSEFAIDQLGDIVFVELADEGATLEKGETFGT
CCCCCCCCCCCCCCCEEECCCCEEEECHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCH
IESVKAVEEVYLPFSGEIVSVNESVIENPELLQSDPIGDGWLVILKPESKASIADLMTSE
HHHHHHHHHHCCCCCCCEEECCHHHHCCCCHHCCCCCCCCEEEEECCCCCCHHHHHHHCH
EYQSKVVPK
HHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA