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The map label for this gene is tig [H]

Identifier: 126697183

GI number: 126697183

Start: 1570001

End: 1571434

Strand: Direct

Name: tig [H]

Synonym: P9301_18451

Alternate gene names: 126697183

Gene position: 1570001-1571434 (Clockwise)

Preceding gene: 126697179

Following gene: 126697184

Centisome position: 95.62

GC content: 30.54

Gene sequence:

>1434_bases
ATGGCTAAAGATGCACTAATAGTCAAAACAACACCTCTGCCTCAAAGTAGAATTTCATTCGAATTAGAAATACCATCTGA
GACATGCAAAACGTGTGTAAATGAAACAATCAGTACCATTAGTCGTTCGGCTAAAATTCCAGGGTTTAGACTTGGTAAGA
TTCCTAAACAAGTCTTAATCCAAAGAATTGGTATCACACAATTACATGCTTCTGCTCTAGAAAAAATCATTGATAAATCC
TGGCAAGAAGCATTAAAAATCAAATCCATAGAGCCACTTAGTGAGCCAGAGTTGGTAGATGGATTTGAATCTTTACTTGC
AAAGTTTAGTCCTGAAAAATCACTTAAAGTTACTCTTCAAACTGATGTTGCACCCGAATTAAAACTAAAAAAATCAAAAG
GACTCAGTGTTGAAATCTCAAAAACTAAATTTGATCCAAAGTCAATAGATGAAGCTCTAGAAAAATCTAGAAATCAGTTT
GCAAACATTATTCCAGTTACCAACAGAGCAGCAAAATTAGGGGATATTGCTGTAGTTAGTTTCAAAGGTAAATATAAAGA
TTCTGGTAAAGAGATTGATGGTGGTACTAGTGAATCAATGGATCTTGAGTTAGAAAAGAACAAAATGATTCCAGGCTTCG
TTGAGGGAATCGTAAAGATGAAAATTGGTGATACTAAAACACTTAACCTTAAATTCCCTGAAGATTATTCTCATGAAGAT
TCAAGAGGCAAAGAAGCAATCTTTGAAGTAAATCTTAAGGATCTTAAGGAAAAAGAATTACCTGAGCTTAATGACGATTT
TGCAAAACAATCTGGAAATAAGGAATCATTAAAAGAGTTAAAGAAAGATATTGAAAAACAACTTAAAGATAATTTTGAAA
AAACTCAAAAAGATATCAAAATTGAAGCTTTACTAGATGCCTTAACAAACGAATTGGTTACTGAAATTCCAAAATCTATG
ATTGATCTAGAAGTGAGAAATAATATTGAACAAACAGCGCAAAGATTTGCTCAACAAGGTCTTGATGTTAAATCTACTTT
CACTCCTGAATTAGTAAAGTCATTAGCAGAGTCTACAAGACCTCAGGCTGAAAAAAATGTTCAAAGAAATTTAGCTCTTA
AAGCATTAGCAGAAAAAGAAAACATAACAATTGATAAAAGTGAAATTGATTTAAAAATGAAAGAATATGATGATGTAATC
TCTCAATCCTCAAAACAAATAGATATTAAAAAATTAACAGAAGTTATAAGTAACGATTTACTCAAAGAAAAGTTAATAAT
TTGGCTTGAAGAAAATTCTGAAGTAAATGAAAAAACTACAAAAACTTCTAAAGCAACCAAAACCTCTAAAACAACAAAAG
CTACAAAAACTGCGACAAAAACTACAAAAACTACAAAAACTACAAAAACTCAAAATAAAAAAGAAAAAAAATAA

Upstream 100 bases:

>100_bases
GATTGACTATTTTTTTTACTTTATCAAAAAATTAACTTTCAAGGAAATCACATAATGTGAAATAATTAAGACTCTGCTCA
TAGTAATAACTTAGAAAAAC

Downstream 100 bases:

>100_bases
TTTATGAAATTTCCTACTAATTAAACAAAAACCCCTTAAATTATCTATAAGGCGAAAACTAATTTGTGAACTCAGAAAAA
AAACATTTAATCCAAAGCTC

Product: trigger factor

Products: peptidylproline (w =0) [C]

Alternate protein names: TF [H]

Number of amino acids: Translated: 477; Mature: 476

Protein sequence:

>477_residues
MAKDALIVKTTPLPQSRISFELEIPSETCKTCVNETISTISRSAKIPGFRLGKIPKQVLIQRIGITQLHASALEKIIDKS
WQEALKIKSIEPLSEPELVDGFESLLAKFSPEKSLKVTLQTDVAPELKLKKSKGLSVEISKTKFDPKSIDEALEKSRNQF
ANIIPVTNRAAKLGDIAVVSFKGKYKDSGKEIDGGTSESMDLELEKNKMIPGFVEGIVKMKIGDTKTLNLKFPEDYSHED
SRGKEAIFEVNLKDLKEKELPELNDDFAKQSGNKESLKELKKDIEKQLKDNFEKTQKDIKIEALLDALTNELVTEIPKSM
IDLEVRNNIEQTAQRFAQQGLDVKSTFTPELVKSLAESTRPQAEKNVQRNLALKALAEKENITIDKSEIDLKMKEYDDVI
SQSSKQIDIKKLTEVISNDLLKEKLIIWLEENSEVNEKTTKTSKATKTSKTTKATKTATKTTKTTKTTKTQNKKEKK

Sequences:

>Translated_477_residues
MAKDALIVKTTPLPQSRISFELEIPSETCKTCVNETISTISRSAKIPGFRLGKIPKQVLIQRIGITQLHASALEKIIDKS
WQEALKIKSIEPLSEPELVDGFESLLAKFSPEKSLKVTLQTDVAPELKLKKSKGLSVEISKTKFDPKSIDEALEKSRNQF
ANIIPVTNRAAKLGDIAVVSFKGKYKDSGKEIDGGTSESMDLELEKNKMIPGFVEGIVKMKIGDTKTLNLKFPEDYSHED
SRGKEAIFEVNLKDLKEKELPELNDDFAKQSGNKESLKELKKDIEKQLKDNFEKTQKDIKIEALLDALTNELVTEIPKSM
IDLEVRNNIEQTAQRFAQQGLDVKSTFTPELVKSLAESTRPQAEKNVQRNLALKALAEKENITIDKSEIDLKMKEYDDVI
SQSSKQIDIKKLTEVISNDLLKEKLIIWLEENSEVNEKTTKTSKATKTSKTTKATKTATKTTKTTKTTKTQNKKEKK
>Mature_476_residues
AKDALIVKTTPLPQSRISFELEIPSETCKTCVNETISTISRSAKIPGFRLGKIPKQVLIQRIGITQLHASALEKIIDKSW
QEALKIKSIEPLSEPELVDGFESLLAKFSPEKSLKVTLQTDVAPELKLKKSKGLSVEISKTKFDPKSIDEALEKSRNQFA
NIIPVTNRAAKLGDIAVVSFKGKYKDSGKEIDGGTSESMDLELEKNKMIPGFVEGIVKMKIGDTKTLNLKFPEDYSHEDS
RGKEAIFEVNLKDLKEKELPELNDDFAKQSGNKESLKELKKDIEKQLKDNFEKTQKDIKIEALLDALTNELVTEIPKSMI
DLEVRNNIEQTAQRFAQQGLDVKSTFTPELVKSLAESTRPQAEKNVQRNLALKALAEKENITIDKSEIDLKMKEYDDVIS
QSSKQIDIKKLTEVISNDLLKEKLIIWLEENSEVNEKTTKTSKATKTSKTTKATKTATKTTKTTKTTKTQNKKEKK

Specific function: Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation [H]

COG id: COG0544

COG function: function code O; FKBP-type peptidyl-prolyl cis-trans isomerase (trigger factor)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase FKBP-type domain [H]

Homologues:

Organism=Escherichia coli, GI1786640, Length=445, Percent_Identity=28.5393258426966, Blast_Score=137, Evalue=1e-33,

Paralogues:

None

Copy number: 1070 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 500 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 340 Molecules/Cell In: Growth-Phase, Mini

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001179
- InterPro:   IPR005215
- InterPro:   IPR008880
- InterPro:   IPR008881 [H]

Pfam domain/function: PF00254 FKBP_C; PF05698 Trigger_C; PF05697 Trigger_N [H]

EC number: NA

Molecular weight: Translated: 53584; Mature: 53453

Theoretical pI: Translated: 9.60; Mature: 9.60

Prosite motif: PS50059 FKBP_PPIASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKDALIVKTTPLPQSRISFELEIPSETCKTCVNETISTISRSAKIPGFRLGKIPKQVLI
CCCCCEEEEECCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCHHHHH
QRIGITQLHASALEKIIDKSWQEALKIKSIEPLSEPELVDGFESLLAKFSPEKSLKVTLQ
HHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEE
TDVAPELKLKKSKGLSVEISKTKFDPKSIDEALEKSRNQFANIIPVTNRAAKLGDIAVVS
CCCCCCCEECCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCEEEEE
FKGKYKDSGKEIDGGTSESMDLELEKNKMIPGFVEGIVKMKIGDTKTLNLKFPEDYSHED
ECCCCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHEEECCCEEEEEECCCCCCCCC
SRGKEAIFEVNLKDLKEKELPELNDDFAKQSGNKESLKELKKDIEKQLKDNFEKTQKDIK
CCCCCEEEECCHHHHHHHCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
IEALLDALTNELVTEIPKSMIDLEVRNNIEQTAQRFAQQGLDVKSTFTPELVKSLAESTR
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCC
PQAEKNVQRNLALKALAEKENITIDKSEIDLKMKEYDDVISQSSKQIDIKKLTEVISNDL
CHHHHHHHHHHHHHHHHCCCCCEEEHHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
LKEKLIIWLEENSEVNEKTTKTSKATKTSKTTKATKTATKTTKTTKTTKTQNKKEKK
HHHHEEEEEECCCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AKDALIVKTTPLPQSRISFELEIPSETCKTCVNETISTISRSAKIPGFRLGKIPKQVLI
CCCCEEEEECCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCHHHHH
QRIGITQLHASALEKIIDKSWQEALKIKSIEPLSEPELVDGFESLLAKFSPEKSLKVTLQ
HHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEE
TDVAPELKLKKSKGLSVEISKTKFDPKSIDEALEKSRNQFANIIPVTNRAAKLGDIAVVS
CCCCCCCEECCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCEEEEE
FKGKYKDSGKEIDGGTSESMDLELEKNKMIPGFVEGIVKMKIGDTKTLNLKFPEDYSHED
ECCCCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHEEECCCEEEEEECCCCCCCCC
SRGKEAIFEVNLKDLKEKELPELNDDFAKQSGNKESLKELKKDIEKQLKDNFEKTQKDIK
CCCCCEEEECCHHHHHHHCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
IEALLDALTNELVTEIPKSMIDLEVRNNIEQTAQRFAQQGLDVKSTFTPELVKSLAESTR
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCC
PQAEKNVQRNLALKALAEKENITIDKSEIDLKMKEYDDVISQSSKQIDIKKLTEVISNDL
CHHHHHHHHHHHHHHHHCCCCCEEEHHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
LKEKLIIWLEENSEVNEKTTKTSKATKTSKTTKATKTATKTTKTTKTTKTQNKKEKK
HHHHEEEEEECCCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: peptidylproline (w =180) [C]

Specific reaction: peptidylproline (w =180) = peptidylproline (w =0) [C]

General reaction: Isomerases; cis-trans-Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA