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The map label for this gene is cobJ [H]

Identifier: 126697055

GI number: 126697055

Start: 1451388

End: 1453190

Strand: Direct

Name: cobJ [H]

Synonym: P9301_17171

Alternate gene names: 126697055

Gene position: 1451388-1453190 (Clockwise)

Preceding gene: 126697051

Following gene: 126697056

Centisome position: 88.4

GC content: 30.89

Gene sequence:

>1803_bases
TTGAAAGGAATTGCTATAGGTCTATCTAATAATTCAAAAGAAATTTTAAAAAGGCTTAAAAAAACCGAATTTGTAAAAGA
TATATATATTGCAGGTTCTTCAAAAGATGATGGAATGCAAAATGAACTTATTCAAGTACAAAAACCTAGAGAAATCTTAC
TTAAAAAATGGCCCGAGATAGATTTAATAATTTTTGTAGGCTCAATTGCTGCATCAATAAGAATAATAAACTCTTTTTTA
ACCTCTAAAGATCAAGACCCAGGCATTATTGTTATAGATAACAAATGTTCCAAGGTAATTCCTTTAATTGGCTTACATCA
GTCAAATTCGCAAAATATTGCATATCAAATTGCTAATTTACTCGGTGGCGAAATAATAGAAACTAATAATTCCAATGATC
AAAGCTTCTTAAATCTTGATGCATTTGGAAATCAATGGGGTTGGAAAAGATCTGGCAAAATAAAGGATTGGTCAAAACTA
GTAATTAAACAAGCTAAGAACGAAGAAATATTTTGCAAACAATCATCTGGAAATAGCTTATGGAATACTTCAGAATCAGG
GGCAATGATTAATCAAATTAATGAAAAAGAAATTGAAAAACTAGATTCAACATTTCATGTGAGTATATTCAAAAATAATG
AAAGAAATTGGCATCCTCCCGTATTGTGGATTGGTATTGGATGTGAAAGAAATACAAGCAAAGAGTTAATAGAAAATTCT
TTAAATAATTTTTTGGAGTCAAGAAATTTATCACAGCAATCAATTGCGGGATTTGCAACTATTGATATAAAAAAAGATGA
AAAAGGGATTTTAGAACTTTCTGAAGAAAAAAACTTGCCTATAAAGTTTTTTAGTAAAGAAGATCTTTCAACAATAATTG
TTCCAAATCCATCAAATGTCGTACAAAATGAAATTGGCACACCTTCCGTAGCAGAAGCCTCTTGCTTACTCGCAGCAGGA
GAAGAATCAAAATTATTAGAAGAAAAAAGAATTTTTAAAAATCAATTTGGGGCAGTAACTATCGCTGTAGCAGAGTCAAA
AAATCAGTATAATCCAACCCATGGTGAAATACATATTATTGGAAGTGGTCCTGGTGATATCTCCTTCCTAACCAGTAATG
CAAAAAAAGCACTTTCAAGATGTACTGTCTGGATCGGATACAAAATGTATTTAGATTTAATTAAGCCCTTAAAAAGGAGT
GATCAAGTTTTAATTAAAAGTAAACTTACTGAAGAAAAAGAGAGATGTAGTAAAGCAATTAAACTAGCGGAGGAAGGAAT
AAAAGTGGCTTTAATTTCTTCAGGTGAATCTGGATTTTATGGCATGGCTGGTTTACTTTTAGAATTGCTTCAAAAAATAA
AAAAAGAATATAGACCTTACTTTCAAGTACACCCAGGTATAAGTAGTGTTCAATTAGCGGCTGCGATTAGTGGAGCACCA
CTAATGAATGACTTTTGTTCAATAAGCTTAAGCGATAAATTAACTCCATGGCCTTTAATAGAAAAAAGAATTGAAGGTGC
TCTAGTGGGTGACTTTGTAATAGCTTTATTTAATCCTCAATCCATTGAAAGAAATTGGCAATTGAAAAGTGTAATAAATA
GATGTTTACAATCTAGGCATATGGATACTCCAGTTTTAATAGCTAGAGAAGTAGGGAGAGAAAATCAAACCAAAAAATTT
TTTACTTTAAACACCATTCCTTTTAAAGAGATTGATATGTTAACAGTCATCATTATTGGCAATTCTCAAACAACTTTAGT
TGACGAAATTTTTCTGACCCCCAGAGGATATTTACAAAATTAA

Upstream 100 bases:

>100_bases
TTTAAATAAATTTCTTAAATTTTTTTTTATATTTCAAAAGAAATTTCTTAAAATCCAGCGAGAGGATATAATTTCATCGT
AACTATCAATAGTTTCTGAT

Downstream 100 bases:

>100_bases
GTTTAGGTAATTGATAATTTTATAAATAGAATTTTTTTCTTTGCTTTTTCTTTATAAGAATACTAATCTTTGATAATAAT
GATGCAATAAAAAAAATTGA

Product: bifunctional cbiH protein and precorrin-3B C17-methyltransferase

Products: S-adenosyl-L-homocysteine; Precorrin 4

Alternate protein names: Cobalt-precorrin-3 methylase; Cobalt-precorrin-3 methyltransferase [H]

Number of amino acids: Translated: 600; Mature: 600

Protein sequence:

>600_residues
MKGIAIGLSNNSKEILKRLKKTEFVKDIYIAGSSKDDGMQNELIQVQKPREILLKKWPEIDLIIFVGSIAASIRIINSFL
TSKDQDPGIIVIDNKCSKVIPLIGLHQSNSQNIAYQIANLLGGEIIETNNSNDQSFLNLDAFGNQWGWKRSGKIKDWSKL
VIKQAKNEEIFCKQSSGNSLWNTSESGAMINQINEKEIEKLDSTFHVSIFKNNERNWHPPVLWIGIGCERNTSKELIENS
LNNFLESRNLSQQSIAGFATIDIKKDEKGILELSEEKNLPIKFFSKEDLSTIIVPNPSNVVQNEIGTPSVAEASCLLAAG
EESKLLEEKRIFKNQFGAVTIAVAESKNQYNPTHGEIHIIGSGPGDISFLTSNAKKALSRCTVWIGYKMYLDLIKPLKRS
DQVLIKSKLTEEKERCSKAIKLAEEGIKVALISSGESGFYGMAGLLLELLQKIKKEYRPYFQVHPGISSVQLAAAISGAP
LMNDFCSISLSDKLTPWPLIEKRIEGALVGDFVIALFNPQSIERNWQLKSVINRCLQSRHMDTPVLIAREVGRENQTKKF
FTLNTIPFKEIDMLTVIIIGNSQTTLVDEIFLTPRGYLQN

Sequences:

>Translated_600_residues
MKGIAIGLSNNSKEILKRLKKTEFVKDIYIAGSSKDDGMQNELIQVQKPREILLKKWPEIDLIIFVGSIAASIRIINSFL
TSKDQDPGIIVIDNKCSKVIPLIGLHQSNSQNIAYQIANLLGGEIIETNNSNDQSFLNLDAFGNQWGWKRSGKIKDWSKL
VIKQAKNEEIFCKQSSGNSLWNTSESGAMINQINEKEIEKLDSTFHVSIFKNNERNWHPPVLWIGIGCERNTSKELIENS
LNNFLESRNLSQQSIAGFATIDIKKDEKGILELSEEKNLPIKFFSKEDLSTIIVPNPSNVVQNEIGTPSVAEASCLLAAG
EESKLLEEKRIFKNQFGAVTIAVAESKNQYNPTHGEIHIIGSGPGDISFLTSNAKKALSRCTVWIGYKMYLDLIKPLKRS
DQVLIKSKLTEEKERCSKAIKLAEEGIKVALISSGESGFYGMAGLLLELLQKIKKEYRPYFQVHPGISSVQLAAAISGAP
LMNDFCSISLSDKLTPWPLIEKRIEGALVGDFVIALFNPQSIERNWQLKSVINRCLQSRHMDTPVLIAREVGRENQTKKF
FTLNTIPFKEIDMLTVIIIGNSQTTLVDEIFLTPRGYLQN
>Mature_600_residues
MKGIAIGLSNNSKEILKRLKKTEFVKDIYIAGSSKDDGMQNELIQVQKPREILLKKWPEIDLIIFVGSIAASIRIINSFL
TSKDQDPGIIVIDNKCSKVIPLIGLHQSNSQNIAYQIANLLGGEIIETNNSNDQSFLNLDAFGNQWGWKRSGKIKDWSKL
VIKQAKNEEIFCKQSSGNSLWNTSESGAMINQINEKEIEKLDSTFHVSIFKNNERNWHPPVLWIGIGCERNTSKELIENS
LNNFLESRNLSQQSIAGFATIDIKKDEKGILELSEEKNLPIKFFSKEDLSTIIVPNPSNVVQNEIGTPSVAEASCLLAAG
EESKLLEEKRIFKNQFGAVTIAVAESKNQYNPTHGEIHIIGSGPGDISFLTSNAKKALSRCTVWIGYKMYLDLIKPLKRS
DQVLIKSKLTEEKERCSKAIKLAEEGIKVALISSGESGFYGMAGLLLELLQKIKKEYRPYFQVHPGISSVQLAAAISGAP
LMNDFCSISLSDKLTPWPLIEKRIEGALVGDFVIALFNPQSIERNWQLKSVINRCLQSRHMDTPVLIAREVGRENQTKKF
FTLNTIPFKEIDMLTVIIIGNSQTTLVDEIFLTPRGYLQN

Specific function: Methyltransferase that catalyzes the methylation of C-17 in cobalt-precorrin-3B to form cobalt-precorrin-4 [H]

COG id: COG1010

COG function: function code H; Precorrin-3B methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006363 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: 2.1.1.131

Molecular weight: Translated: 67128; Mature: 67128

Theoretical pI: Translated: 8.36; Mature: 8.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGIAIGLSNNSKEILKRLKKTEFVKDIYIAGSSKDDGMQNELIQVQKPREILLKKWPEI
CCCEEEEECCCHHHHHHHHHHHHHHHEEEEECCCCCCCCHHHHEECCCCHHHHHHCCCCE
DLIIFVGSIAASIRIINSFLTSKDQDPGIIVIDNKCSKVIPLIGLHQSNSQNIAYQIANL
EEEEEEHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCEEEECCCCCCHHHHHHHHH
LGGEIIETNNSNDQSFLNLDAFGNQWGWKRSGKIKDWSKLVIKQAKNEEIFCKQSSGNSL
HCCEEEECCCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCC
WNTSESGAMINQINEKEIEKLDSTFHVSIFKNNERNWHPPVLWIGIGCERNTSKELIENS
CCCCCCCHHHHHCCHHHHHHHCCEEEEEEEECCCCCCCCCEEEEEECCCCCCHHHHHHHH
LNNFLESRNLSQQSIAGFATIDIKKDEKGILELSEEKNLPIKFFSKEDLSTIIVPNPSNV
HHHHHHHCCCCHHHCCEEEEEEEECCCCCCEEECCCCCCCEEEECCCCCCEEEECCCHHH
VQNEIGTPSVAEASCLLAAGEESKLLEEKRIFKNQFGAVTIAVAESKNQYNPTHGEIHII
HHHCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCEEEEE
GSGPGDISFLTSNAKKALSRCTVWIGYKMYLDLIKPLKRSDQVLIKSKLTEEKERCSKAI
ECCCCCEEEEECHHHHHHHHCCCEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
KLAEEGIKVALISSGESGFYGMAGLLLELLQKIKKEYRPYFQVHPGISSVQLAAAISGAP
HHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCCEEHHHHHCCCC
LMNDFCSISLSDKLTPWPLIEKRIEGALVGDFVIALFNPQSIERNWQLKSVINRCLQSRH
HHHHHHCEEECCCCCCCHHHHHHHCCCEEEEEEEEEECCHHHCCCCCHHHHHHHHHHHCC
MDTPVLIAREVGRENQTKKFFTLNTIPFKEIDMLTVIIIGNSQTTLVDEIFLTPRGYLQN
CCCCCHHHHHHCCCCCCCEEEEEECCCCCCCCEEEEEEECCCCCEEEEHEEECCCHHCCC
>Mature Secondary Structure
MKGIAIGLSNNSKEILKRLKKTEFVKDIYIAGSSKDDGMQNELIQVQKPREILLKKWPEI
CCCEEEEECCCHHHHHHHHHHHHHHHEEEEECCCCCCCCHHHHEECCCCHHHHHHCCCCE
DLIIFVGSIAASIRIINSFLTSKDQDPGIIVIDNKCSKVIPLIGLHQSNSQNIAYQIANL
EEEEEEHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCEEEECCCCCCHHHHHHHHH
LGGEIIETNNSNDQSFLNLDAFGNQWGWKRSGKIKDWSKLVIKQAKNEEIFCKQSSGNSL
HCCEEEECCCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCC
WNTSESGAMINQINEKEIEKLDSTFHVSIFKNNERNWHPPVLWIGIGCERNTSKELIENS
CCCCCCCHHHHHCCHHHHHHHCCEEEEEEEECCCCCCCCCEEEEEECCCCCCHHHHHHHH
LNNFLESRNLSQQSIAGFATIDIKKDEKGILELSEEKNLPIKFFSKEDLSTIIVPNPSNV
HHHHHHHCCCCHHHCCEEEEEEEECCCCCCEEECCCCCCCEEEECCCCCCEEEECCCHHH
VQNEIGTPSVAEASCLLAAGEESKLLEEKRIFKNQFGAVTIAVAESKNQYNPTHGEIHII
HHHCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCEEEEE
GSGPGDISFLTSNAKKALSRCTVWIGYKMYLDLIKPLKRSDQVLIKSKLTEEKERCSKAI
ECCCCCEEEEECHHHHHHHHCCCEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
KLAEEGIKVALISSGESGFYGMAGLLLELLQKIKKEYRPYFQVHPGISSVQLAAAISGAP
HHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCCEEHHHHHCCCC
LMNDFCSISLSDKLTPWPLIEKRIEGALVGDFVIALFNPQSIERNWQLKSVINRCLQSRH
HHHHHHCEEECCCCCCCHHHHHHHCCCEEEEEEEEEECCHHHCCCCCHHHHHHHHHHHCC
MDTPVLIAREVGRENQTKKFFTLNTIPFKEIDMLTVIIIGNSQTTLVDEIFLTPRGYLQN
CCCCCHHHHHHCCCCCCCEEEEEECCCCCCCCEEEEEEECCCCCEEEEHEEECCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-adenosyl-L-methionine; Precorrin 3B

Specific reaction: S-adenosyl-L-methionine + precorrin-3B = S-adenosyl-L-homocysteine + precorrin-4

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8501034; 11677609 [H]