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The map label for this gene is lipA1 [H]

Identifier: 126697044

GI number: 126697044

Start: 1440407

End: 1441300

Strand: Reverse

Name: lipA1 [H]

Synonym: P9301_17061

Alternate gene names: 126697044

Gene position: 1441300-1440407 (Counterclockwise)

Preceding gene: 126697047

Following gene: 126697041

Centisome position: 87.78

GC content: 36.02

Gene sequence:

>894_bases
GTGACTAATAATCCCAATAGTTTAATTTCTAAACCTGAATGGTTAAGGGTCAAAGCTCCACAAGTTGAGCGAATTGGTAA
TACTGCAAATTTATTAAATGATTTAAATCTCAATACCGTATGTCAAGAAGCAAGCTGTCCAAATATCGGCGAATGTTTTG
CTAGTGGAACTGCAACTTTCCTCATAATGGGTCCTGGCTGCACTAGGGCATGTCCATACTGCGATATTGATTTTGATAGA
TCTAAGAGAGACTTAGACCCCACTGAACCATATCGTTTAGCTGAAGCTGTTTTTAGAATGAAGCTTAAGCATGTTGTAAT
CACATCAGTTAATAGAGACGATCTTGAGGATGGTGGCGCATCTCAATTTTTTCAATGTGTTCATCAAATAAGAGAAAAGT
CTCCTGAAACTACTATTGAGCTTCTAATACCTGATCTTTGTGGCAACTGGAAAGCGCTTGAAAAAGTACTTGATTCAAAT
CCAAACGTTTTAAACCACAATATTGAGACTGTGTCTTCGCTATATAAAAAAGTAAGACCTCAAGGCAAATATGAAAGAAC
TCTTGAGTTGCTCAAAAGAACCAGAGACTATTCTCCTAAAATTTATACAAAGTCTGGCTTCATGCTTGGTTTAGGGGAAA
AAGACGAGGAGGTCTTAAGTCTGCTTAAGGATTTAAAAAGTAATTTCGTTGATATTGTTACTATTGGCCAATATTTATCT
CCTGGCCCTAATCATTTACCTGTTAAAAGATTTGTAAGTCCTTCAAAATTTGACTACTTTAAAGCGTTCGGGGAAAAAGA
TTTAAACTTCATGCAAGTAGTTAGTTCTCCTTTAACTCGTAGTAGCTACCATGCTGAAGAGATTCAAAAACTTATGAAAA
AGTATCCAAGATAG

Upstream 100 bases:

>100_bases
AATTTTTTACTTCTGTTGAATGAGCTTCATCTTTGAAATTATAAATAGTTAATTCATCTTGTTTTTAGTAAAATAAAATT
ACAGGTCAAGAAGATTTAAT

Downstream 100 bases:

>100_bases
TTATATTGATTTATTTGGATCTACCCACTCATTTAATTTCCATTCAACTAGATCATTTTTAATCATTGGATCATTCTTAA
TTATTTTTAGTGCATTTCTA

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn 1; Lipoate synthase 1; Lipoic acid synthase 1; Sulfur insertion protein lipA1 [H]

Number of amino acids: Translated: 297; Mature: 296

Protein sequence:

>297_residues
MTNNPNSLISKPEWLRVKAPQVERIGNTANLLNDLNLNTVCQEASCPNIGECFASGTATFLIMGPGCTRACPYCDIDFDR
SKRDLDPTEPYRLAEAVFRMKLKHVVITSVNRDDLEDGGASQFFQCVHQIREKSPETTIELLIPDLCGNWKALEKVLDSN
PNVLNHNIETVSSLYKKVRPQGKYERTLELLKRTRDYSPKIYTKSGFMLGLGEKDEEVLSLLKDLKSNFVDIVTIGQYLS
PGPNHLPVKRFVSPSKFDYFKAFGEKDLNFMQVVSSPLTRSSYHAEEIQKLMKKYPR

Sequences:

>Translated_297_residues
MTNNPNSLISKPEWLRVKAPQVERIGNTANLLNDLNLNTVCQEASCPNIGECFASGTATFLIMGPGCTRACPYCDIDFDR
SKRDLDPTEPYRLAEAVFRMKLKHVVITSVNRDDLEDGGASQFFQCVHQIREKSPETTIELLIPDLCGNWKALEKVLDSN
PNVLNHNIETVSSLYKKVRPQGKYERTLELLKRTRDYSPKIYTKSGFMLGLGEKDEEVLSLLKDLKSNFVDIVTIGQYLS
PGPNHLPVKRFVSPSKFDYFKAFGEKDLNFMQVVSSPLTRSSYHAEEIQKLMKKYPR
>Mature_296_residues
TNNPNSLISKPEWLRVKAPQVERIGNTANLLNDLNLNTVCQEASCPNIGECFASGTATFLIMGPGCTRACPYCDIDFDRS
KRDLDPTEPYRLAEAVFRMKLKHVVITSVNRDDLEDGGASQFFQCVHQIREKSPETTIELLIPDLCGNWKALEKVLDSNP
NVLNHNIETVSSLYKKVRPQGKYERTLELLKRTRDYSPKIYTKSGFMLGLGEKDEEVLSLLKDLKSNFVDIVTIGQYLSP
GPNHLPVKRFVSPSKFDYFKAFGEKDLNFMQVVSSPLTRSSYHAEEIQKLMKKYPR

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives [H]

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family [H]

Homologues:

Organism=Homo sapiens, GI37577166, Length=283, Percent_Identity=42.4028268551237, Blast_Score=242, Evalue=2e-64,
Organism=Homo sapiens, GI37577164, Length=243, Percent_Identity=42.798353909465, Blast_Score=211, Evalue=8e-55,
Organism=Escherichia coli, GI1786846, Length=289, Percent_Identity=46.0207612456747, Blast_Score=275, Evalue=3e-75,
Organism=Caenorhabditis elegans, GI32564533, Length=256, Percent_Identity=44.921875, Blast_Score=228, Evalue=3e-60,
Organism=Saccharomyces cerevisiae, GI6324770, Length=291, Percent_Identity=40.5498281786942, Blast_Score=228, Evalue=7e-61,
Organism=Drosophila melanogaster, GI221513272, Length=283, Percent_Identity=40.6360424028269, Blast_Score=225, Evalue=3e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.8.1.8 [H]

Molecular weight: Translated: 33731; Mature: 33600

Theoretical pI: Translated: 8.19; Mature: 8.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNNPNSLISKPEWLRVKAPQVERIGNTANLLNDLNLNTVCQEASCPNIGECFASGTATF
CCCCCCHHHCCCCEEEECCCHHHHHCCHHHHHHCCCHHHHHHCCCCCCHHHHHCCCCEEE
LIMGPGCTRACPYCDIDFDRSKRDLDPTEPYRLAEAVFRMKLKHVVITSVNRDDLEDGGA
EEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHEECCCHHCCCCCCH
SQFFQCVHQIREKSPETTIELLIPDLCGNWKALEKVLDSNPNVLNHNIETVSSLYKKVRP
HHHHHHHHHHHHCCCCCEEEHEEHHHCCCHHHHHHHHCCCCCEECCCHHHHHHHHHHHCC
QGKYERTLELLKRTRDYSPKIYTKSGFMLGLGEKDEEVLSLLKDLKSNFVDIVTIGQYLS
CCHHHHHHHHHHHHHCCCCEEEECCCCEEECCCCHHHHHHHHHHHHHCCEEHHHHHHHHC
PGPNHLPVKRFVSPSKFDYFKAFGEKDLNFMQVVSSPLTRSSYHAEEIQKLMKKYPR
CCCCCCCHHHHCCCCHHHHHHHHCCCCCHHHHHHHCCHHCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure 
TNNPNSLISKPEWLRVKAPQVERIGNTANLLNDLNLNTVCQEASCPNIGECFASGTATF
CCCCCHHHCCCCEEEECCCHHHHHCCHHHHHHCCCHHHHHHCCCCCCHHHHHCCCCEEE
LIMGPGCTRACPYCDIDFDRSKRDLDPTEPYRLAEAVFRMKLKHVVITSVNRDDLEDGGA
EEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHEECCCHHCCCCCCH
SQFFQCVHQIREKSPETTIELLIPDLCGNWKALEKVLDSNPNVLNHNIETVSSLYKKVRP
HHHHHHHHHHHHCCCCCEEEHEEHHHCCCHHHHHHHHCCCCCEECCCHHHHHHHHHHHCC
QGKYERTLELLKRTRDYSPKIYTKSGFMLGLGEKDEEVLSLLKDLKSNFVDIVTIGQYLS
CCHHHHHHHHHHHHHCCCCEEEECCCCEEECCCCHHHHHHHHHHHHHCCEEHHHHHHHHC
PGPNHLPVKRFVSPSKFDYFKAFGEKDLNFMQVVSSPLTRSSYHAEEIQKLMKKYPR
CCCCCCCHHHHCCCCHHHHHHHHCCCCCHHHHHHHCCHHCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12917642 [H]