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The map label for this gene is rne [H]

Identifier: 126697030

GI number: 126697030

Start: 1425201

End: 1427009

Strand: Direct

Name: rne [H]

Synonym: P9301_16921

Alternate gene names: 126697030

Gene position: 1425201-1427009 (Clockwise)

Preceding gene: 126697027

Following gene: 126697031

Centisome position: 86.8

GC content: 30.46

Gene sequence:

>1809_bases
ATGTCTCAGCAAATTATCATCGCTGAGCAGGCTCGAATTGCAGCACTACTCACAGATGATCGAGTTGATGAATTAATCGT
CGCACAAGGTCATTATCAAATTGGCGATATTTTTTTAGGAACAGTTGAAAATGTTCTCCCTGGAATCGATGCCGCTTTCA
TAAATATTGGTGAAAGTGATAAAAACGGATTCATCCATGTTTCAGATTTAGGTCCACTAAGACTAAAAAAAGGGACATTT
GGAATAACTGAATTATTAGAACCAAAACAAAAAGTTCTAGTACAAGTAATAAAGGAACCCACAGGATCTAAAGGGCCTAG
ACTAACTGGGAGTATTTCAATACCAGGAAAATACATGATATTACAGCCATATGGCCAAGGAGTAAATATTTCAAGAAAAA
TAAATACAGAAACAGAACGAAATCGCTTGAAAGCTCTTGGGGTTTTAATAAAACCTCCAAGCACAGGCTTGTTATTTAGA
ACAGAGGCTGAAAAGATAAAAGAAGAACTTCTAATTGAAGATTTAGAACAAATAATTCAACAATGGGAAAATATACTAAA
AGTTTCTGAAGCTTCTCATCCGCCAAATTTAGTAAAAAGAGATGAAGATTTCTCTCTAAAAATTTTGAGAGATCATATTA
AAGAATCAACTAAAAGCATAATTATCGACAGTAAATTTTCAGTTGCAAGGGCAAAAGACTATTTAAAAAATTATGAATCT
GATATAAATATTGAATTTCACGATAACAATTCATCCCCACATGTTTTAGATGAATACAAAATTAAGAAAACAATTCAAAC
AGCCCTCCAACCTAGAGTAGATCTTCCCTCGGGGGGTTATATAATTATTGAACCTACTGAAGCTTTAACTGTAATCGATG
TAAACTCTGGATCATTTACAAGATCGGCCAACTCAAGACAAACCGTTTTGTGGACGAATTGCGAAGCAGCAGTTGAAATC
TCAAGACAAATGAAGTTAAGAAATATTGGTGGAGTTATTGTAGTTGATTTTATTGATATGGAATCTAGAAGAGATCAATT
TCAGTTACTTGAGCATTTTACTTCAGCAATAAAAGATGATTCTGCTAGGCCTCAAATAGCTCAACTTACAGAATTAGGAT
TGGTAGAGTTAACAAGAAAAAGACAAGGTCAAAATATATATGAATTGTTTGGTAAAAAATGTTCTACATGCGATGGGACA
GGGCATGTAGAAAATATATTAAATCAAGAAATTTCTAACTTAAAAATTAAAAATGATGAAAATAAATTTAATCAATCAGA
CAATCTAAAATCTCAAAATATATATACATCACAATCAACTGATGAGCAGGAAAAAATAATTAACAAGGAATTACTTAACT
CCAAAGACCTAAGTAAAGAGAATTCTTCTTATAAAAAAGAAAATGATAATGATAATTTGAACCAATCAAATTCAAAGGAA
AAAAATACAATAACAGTTGATCTTACTAATGAAGAAAAAATTGTTTTCAGTCAATTAGGTATTAATCCTCTTATAAAATT
AGGTAAAGAATATCTAACTAGCAATAATTTTGTGCGTTTAAAAGACAGTAATAAGGAAAAGGAAAAAACCTTAGATAAAA
AGACAAAATCAAAACAAATTAAAAAAAGCTCAAAATCGGGAGAAGAAAAGATTCAAATTACTATTGAAGATAAAGCAAAT
TCGAAAGACAACTCAACAAATAAAATTAATGAAGTTATATTTACAGAAGAAAAGGACGAAATTGAAGCTACAGATGATCT
AAAAAATGCAAGAAAAAAAAGAAGAAGATCTTCAGCAAGTATTGAATAA

Upstream 100 bases:

>100_bases
TAAAAAAACAATAGCTATAAAAATTGTTTTATTAAAAGAAAAAAATCTAATTTTTTAATTTAGATGATTTTCAAAATCCA
AATCACTTAGAATCAATTAT

Downstream 100 bases:

>100_bases
TGTATCCGAAGTTGGGATAGATGAAGTGGGAAGGGGATCAATTTTTGGTCCAGTTTTTTCAGCAGTTGTTGAATTGACTG
AAAAAAATAAATTTACTTTA

Product: ribonuclease E/G

Products: NA

Alternate protein names: RNase E [H]

Number of amino acids: Translated: 602; Mature: 601

Protein sequence:

>602_residues
MSQQIIIAEQARIAALLTDDRVDELIVAQGHYQIGDIFLGTVENVLPGIDAAFINIGESDKNGFIHVSDLGPLRLKKGTF
GITELLEPKQKVLVQVIKEPTGSKGPRLTGSISIPGKYMILQPYGQGVNISRKINTETERNRLKALGVLIKPPSTGLLFR
TEAEKIKEELLIEDLEQIIQQWENILKVSEASHPPNLVKRDEDFSLKILRDHIKESTKSIIIDSKFSVARAKDYLKNYES
DINIEFHDNNSSPHVLDEYKIKKTIQTALQPRVDLPSGGYIIIEPTEALTVIDVNSGSFTRSANSRQTVLWTNCEAAVEI
SRQMKLRNIGGVIVVDFIDMESRRDQFQLLEHFTSAIKDDSARPQIAQLTELGLVELTRKRQGQNIYELFGKKCSTCDGT
GHVENILNQEISNLKIKNDENKFNQSDNLKSQNIYTSQSTDEQEKIINKELLNSKDLSKENSSYKKENDNDNLNQSNSKE
KNTITVDLTNEEKIVFSQLGINPLIKLGKEYLTSNNFVRLKDSNKEKEKTLDKKTKSKQIKKSSKSGEEKIQITIEDKAN
SKDNSTNKINEVIFTEEKDEIEATDDLKNARKKRRRSSASIE

Sequences:

>Translated_602_residues
MSQQIIIAEQARIAALLTDDRVDELIVAQGHYQIGDIFLGTVENVLPGIDAAFINIGESDKNGFIHVSDLGPLRLKKGTF
GITELLEPKQKVLVQVIKEPTGSKGPRLTGSISIPGKYMILQPYGQGVNISRKINTETERNRLKALGVLIKPPSTGLLFR
TEAEKIKEELLIEDLEQIIQQWENILKVSEASHPPNLVKRDEDFSLKILRDHIKESTKSIIIDSKFSVARAKDYLKNYES
DINIEFHDNNSSPHVLDEYKIKKTIQTALQPRVDLPSGGYIIIEPTEALTVIDVNSGSFTRSANSRQTVLWTNCEAAVEI
SRQMKLRNIGGVIVVDFIDMESRRDQFQLLEHFTSAIKDDSARPQIAQLTELGLVELTRKRQGQNIYELFGKKCSTCDGT
GHVENILNQEISNLKIKNDENKFNQSDNLKSQNIYTSQSTDEQEKIINKELLNSKDLSKENSSYKKENDNDNLNQSNSKE
KNTITVDLTNEEKIVFSQLGINPLIKLGKEYLTSNNFVRLKDSNKEKEKTLDKKTKSKQIKKSSKSGEEKIQITIEDKAN
SKDNSTNKINEVIFTEEKDEIEATDDLKNARKKRRRSSASIE
>Mature_601_residues
SQQIIIAEQARIAALLTDDRVDELIVAQGHYQIGDIFLGTVENVLPGIDAAFINIGESDKNGFIHVSDLGPLRLKKGTFG
ITELLEPKQKVLVQVIKEPTGSKGPRLTGSISIPGKYMILQPYGQGVNISRKINTETERNRLKALGVLIKPPSTGLLFRT
EAEKIKEELLIEDLEQIIQQWENILKVSEASHPPNLVKRDEDFSLKILRDHIKESTKSIIIDSKFSVARAKDYLKNYESD
INIEFHDNNSSPHVLDEYKIKKTIQTALQPRVDLPSGGYIIIEPTEALTVIDVNSGSFTRSANSRQTVLWTNCEAAVEIS
RQMKLRNIGGVIVVDFIDMESRRDQFQLLEHFTSAIKDDSARPQIAQLTELGLVELTRKRQGQNIYELFGKKCSTCDGTG
HVENILNQEISNLKIKNDENKFNQSDNLKSQNIYTSQSTDEQEKIINKELLNSKDLSKENSSYKKENDNDNLNQSNSKEK
NTITVDLTNEEKIVFSQLGINPLIKLGKEYLTSNNFVRLKDSNKEKEKTLDKKTKSKQIKKSSKSGEEKIQITIEDKANS
KDNSTNKINEVIFTEEKDEIEATDDLKNARKKRRRSSASIE

Specific function: Involved In The Processing Of The 5'End Of 16s Rrna. Could Be Involved In Chromosome Segregation And Cell Division. It May Be One Of The Components Of The Cytoplasmic Axial Filaments Bundles Or Merely Regulate The Formation Of This Structure. [C]

COG id: COG1530

COG function: function code J; Ribonucleases G and E

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI87082235, Length=404, Percent_Identity=32.1782178217822, Blast_Score=213, Evalue=3e-56,
Organism=Escherichia coli, GI1787325, Length=416, Percent_Identity=33.4134615384615, Blast_Score=211, Evalue=8e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR019307
- InterPro:   IPR022967
- InterPro:   IPR004659 [H]

Pfam domain/function: PF10150 RNase_E_G; PF00575 S1 [H]

EC number: =3.1.26.12 [H]

Molecular weight: Translated: 68044; Mature: 67913

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQQIIIAEQARIAALLTDDRVDELIVAQGHYQIGDIFLGTVENVLPGIDAAFINIGESD
CCCCEEEECCCEEEEEECCCCCCCEEEECCCCEEHHHHHHHHHHHHCCCCEEEEECCCCC
KNGFIHVSDLGPLRLKKGTFGITELLEPKQKVLVQVIKEPTGSKGPRLTGSISIPGKYMI
CCCEEEEECCCCEEEECCCCCHHHHHCHHHHHHHHHHHCCCCCCCCEEEEEEECCCEEEE
LQPYGQGVNISRKINTETERNRLKALGVLIKPPSTGLLFRTEAEKIKEELLIEDLEQIIQ
ECCCCCCCEEEEECCCHHHHHHHHHEEEEECCCCCCEEEECHHHHHHHHHHHHHHHHHHH
QWENILKVSEASHPPNLVKRDEDFSLKILRDHIKESTKSIIIDSKFSVARAKDYLKNYES
HHHHHHHHHCCCCCCHHHCCCCCCHHHHHHHHHHHHHHEEEEECCCHHHHHHHHHHCCCC
DINIEFHDNNSSPHVLDEYKIKKTIQTALQPRVDLPSGGYIIIEPTEALTVIDVNSGSFT
CEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCEEEEEEECCCCEE
RSANSRQTVLWTNCEAAVEISRQMKLRNIGGVIVVDFIDMESRRDQFQLLEHFTSAIKDD
ECCCCCEEEEEECCHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCC
SARPQIAQLTELGLVELTRKRQGQNIYELFGKKCSTCDGTGHVENILNQEISNLKIKNDE
CCCCHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCEEECCC
NKFNQSDNLKSQNIYTSQSTDEQEKIINKELLNSKDLSKENSSYKKENDNDNLNQSNSKE
CCCCCCCCCCCCCEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC
KNTITVDLTNEEKIVFSQLGINPLIKLGKEYLTSNNFVRLKDSNKEKEKTLDKKTKSKQI
CCEEEEEECCCCEEEHHHCCCHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHH
KKSSKSGEEKIQITIEDKANSKDNSTNKINEVIFTEEKDEIEATDDLKNARKKRRRSSAS
HHHCCCCCEEEEEEEECCCCCCCCCCHHHHHEEEECCCCCCCCHHHHHHHHHHHHHHCCC
IE
CC
>Mature Secondary Structure 
SQQIIIAEQARIAALLTDDRVDELIVAQGHYQIGDIFLGTVENVLPGIDAAFINIGESD
CCCEEEECCCEEEEEECCCCCCCEEEECCCCEEHHHHHHHHHHHHCCCCEEEEECCCCC
KNGFIHVSDLGPLRLKKGTFGITELLEPKQKVLVQVIKEPTGSKGPRLTGSISIPGKYMI
CCCEEEEECCCCEEEECCCCCHHHHHCHHHHHHHHHHHCCCCCCCCEEEEEEECCCEEEE
LQPYGQGVNISRKINTETERNRLKALGVLIKPPSTGLLFRTEAEKIKEELLIEDLEQIIQ
ECCCCCCCEEEEECCCHHHHHHHHHEEEEECCCCCCEEEECHHHHHHHHHHHHHHHHHHH
QWENILKVSEASHPPNLVKRDEDFSLKILRDHIKESTKSIIIDSKFSVARAKDYLKNYES
HHHHHHHHHCCCCCCHHHCCCCCCHHHHHHHHHHHHHHEEEEECCCHHHHHHHHHHCCCC
DINIEFHDNNSSPHVLDEYKIKKTIQTALQPRVDLPSGGYIIIEPTEALTVIDVNSGSFT
CEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCEEEEEEECCCCEE
RSANSRQTVLWTNCEAAVEISRQMKLRNIGGVIVVDFIDMESRRDQFQLLEHFTSAIKDD
ECCCCCEEEEEECCHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCC
SARPQIAQLTELGLVELTRKRQGQNIYELFGKKCSTCDGTGHVENILNQEISNLKIKNDE
CCCCHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCEEECCC
NKFNQSDNLKSQNIYTSQSTDEQEKIINKELLNSKDLSKENSSYKKENDNDNLNQSNSKE
CCCCCCCCCCCCCEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC
KNTITVDLTNEEKIVFSQLGINPLIKLGKEYLTSNNFVRLKDSNKEKEKTLDKKTKSKQI
CCEEEEEECCCCEEEHHHCCCHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHH
KKSSKSGEEKIQITIEDKANSKDNSTNKINEVIFTEEKDEIEATDDLKNARKKRRRSSAS
HHHCCCCCEEEEEEEECCCCCCCCCCHHHHHEEEECCCCCCCCHHHHHHHHHHHHHHCCC
IE
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]