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The map label for this gene is nusA [H]

Identifier: 126697021

GI number: 126697021

Start: 1414567

End: 1415970

Strand: Direct

Name: nusA [H]

Synonym: P9301_16831

Alternate gene names: 126697021

Gene position: 1414567-1415970 (Clockwise)

Preceding gene: 126697020

Following gene: 126697022

Centisome position: 86.16

GC content: 38.03

Gene sequence:

>1404_bases
ATGGCATTAGTTATTCTCCCAGGTTTAAACAATCTTATTGAAGACATTAGTGAGGAAAAAAAATTACCTCCTAATATCGT
TGAAGCAGCCTTGCGCGAAGCTTTGTTAAAGGGATATGAAAAATATAGAAGAACTTTTTACATTGGAGTTAACGAAGATC
CATTTGATGAAGAGTACTTCAGTAATTTTGATGTTGGACTAGATCTAGATGAAGAGGGTTACAGGATATTATCTAGTAAA
ATAATTGTTGAAGAAGTAGAGAGCGAAGATCATCAAATATCTCTAATAGAAGTTAAACAAGTTGCTGATGATGCGCAAAT
AGGTGACACAGTTGTATTAGATGTCACTCCTGAAAAAGAGGATTTTGGGCGAATGGCTGCTTCAACAACAAAGCAAGTTT
TAGCACAAAAATTAAGGGATCAACAACGAAAAATGATCCAAGAAGAATTTGCAGATTTGGAGGATCCTGTTTTAACTGCA
AGAGTTATCAGATTTGAAAGACAATCAGTGATTATGGGAGTTAGTTCGGGAATCGGCAGACCCGAGGTTGAAGCAGAACT
TCCCAAGAGAGATCAATTACCAAATGATAACTATAGAGCAAATGCAACTTTCAAAGTATTTTTAAAAGAAGTTAGCGAAA
TTGCTAGAAAAGGTCCACAACTTTTTGTGAGTAGAGCTAACGCTGGTTTAGTAGTTTATTTATTTGAAAATGAAGTACCG
GAAATTCAGGAAGGTACAGTAAAAATAGTTGCTGTTTCAAGAGAAGCGAATCCTCCTTCAAGAGCTGTTGGGCCAAGAAC
AAAAGTAGCTGTTGATAGCGTCGAAAATGAGGTCGACCCTGTAGGTGCTTGTATTGGAGCGAGAGGAGCAAGAATCCAAC
AAGTAGTTAATGAATTAAGAGGAGAAAAAATTGATGTTATTAAATGGTCATCTGACCCAATACAGTATATTTTGAACTCC
TTAAGTCCGGCTAAAGTCGATCTCGTGAGACTTGTTGACCCTGAAGGTCAACACGCGCATGTACTAGTTCCTCCTGATCA
ATTAAGTCTCGCAATTGGTAGAGAAGGACAAAATGTAAGACTTGCGGCAAGATTAACTGGTTGGAAGATTGATGTTAAAA
ACTCACATGAATACGATCAGGAAGCAGAAGATGCTGCAGTCTCTGAATTAATTATCCAAAGAGAAGATGAAGAGAAACTC
CAGCGTGAAGCTGAGCTTAGATTAGAAGCAGAACAAGCTGAGCGTGCTGCGGAAGATGCAAGATTAAGAGAGCTTTATCC
CCTTCCCGAAGATGAAGAAGAATATGGAGAGGAACAATACGAAGGAGAAGAATTAACAGATAATGATCCATTAGAGACTC
TTCAAGATACTGACATATCTGCCAAAGAGGAGAAAAAACGGTGA

Upstream 100 bases:

>100_bases
TAGGATAAAGAAAATCCCTTTTGATGAAGTATTAAAAATTAGTCTTTGTACCTTAAAAGATTGATTATTTTTCAACCATT
ATTCAATTTTCCCATCATAG

Downstream 100 bases:

>100_bases
TACAACAAAATCCTGTTTTGCGTATGTGCATTTCATGTAGAAAGACATATGACAGGAAAGATCTTTTAAAGATTACTAAA
GATCATAAGCAAGGCATCAT

Product: transcription elongation factor NusA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 467; Mature: 466

Protein sequence:

>467_residues
MALVILPGLNNLIEDISEEKKLPPNIVEAALREALLKGYEKYRRTFYIGVNEDPFDEEYFSNFDVGLDLDEEGYRILSSK
IIVEEVESEDHQISLIEVKQVADDAQIGDTVVLDVTPEKEDFGRMAASTTKQVLAQKLRDQQRKMIQEEFADLEDPVLTA
RVIRFERQSVIMGVSSGIGRPEVEAELPKRDQLPNDNYRANATFKVFLKEVSEIARKGPQLFVSRANAGLVVYLFENEVP
EIQEGTVKIVAVSREANPPSRAVGPRTKVAVDSVENEVDPVGACIGARGARIQQVVNELRGEKIDVIKWSSDPIQYILNS
LSPAKVDLVRLVDPEGQHAHVLVPPDQLSLAIGREGQNVRLAARLTGWKIDVKNSHEYDQEAEDAAVSELIIQREDEEKL
QREAELRLEAEQAERAAEDARLRELYPLPEDEEEYGEEQYEGEELTDNDPLETLQDTDISAKEEKKR

Sequences:

>Translated_467_residues
MALVILPGLNNLIEDISEEKKLPPNIVEAALREALLKGYEKYRRTFYIGVNEDPFDEEYFSNFDVGLDLDEEGYRILSSK
IIVEEVESEDHQISLIEVKQVADDAQIGDTVVLDVTPEKEDFGRMAASTTKQVLAQKLRDQQRKMIQEEFADLEDPVLTA
RVIRFERQSVIMGVSSGIGRPEVEAELPKRDQLPNDNYRANATFKVFLKEVSEIARKGPQLFVSRANAGLVVYLFENEVP
EIQEGTVKIVAVSREANPPSRAVGPRTKVAVDSVENEVDPVGACIGARGARIQQVVNELRGEKIDVIKWSSDPIQYILNS
LSPAKVDLVRLVDPEGQHAHVLVPPDQLSLAIGREGQNVRLAARLTGWKIDVKNSHEYDQEAEDAAVSELIIQREDEEKL
QREAELRLEAEQAERAAEDARLRELYPLPEDEEEYGEEQYEGEELTDNDPLETLQDTDISAKEEKKR
>Mature_466_residues
ALVILPGLNNLIEDISEEKKLPPNIVEAALREALLKGYEKYRRTFYIGVNEDPFDEEYFSNFDVGLDLDEEGYRILSSKI
IVEEVESEDHQISLIEVKQVADDAQIGDTVVLDVTPEKEDFGRMAASTTKQVLAQKLRDQQRKMIQEEFADLEDPVLTAR
VIRFERQSVIMGVSSGIGRPEVEAELPKRDQLPNDNYRANATFKVFLKEVSEIARKGPQLFVSRANAGLVVYLFENEVPE
IQEGTVKIVAVSREANPPSRAVGPRTKVAVDSVENEVDPVGACIGARGARIQQVVNELRGEKIDVIKWSSDPIQYILNSL
SPAKVDLVRLVDPEGQHAHVLVPPDQLSLAIGREGQNVRLAARLTGWKIDVKNSHEYDQEAEDAAVSELIIQREDEEKLQ
REAELRLEAEQAERAAEDARLRELYPLPEDEEEYGEEQYEGEELTDNDPLETLQDTDISAKEEKKR

Specific function: Participates in both the termination and antitermination of transcription. Interacts with RNA polymerase and binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=363, Percent_Identity=33.0578512396694, Blast_Score=185, Evalue=6e-48,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N; PF00575 S1 [H]

EC number: NA

Molecular weight: Translated: 52641; Mature: 52509

Theoretical pI: Translated: 4.25; Mature: 4.25

Prosite motif: PS50084 KH_TYPE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALVILPGLNNLIEDISEEKKLPPNIVEAALREALLKGYEKYRRTFYIGVNEDPFDEEYF
CEEEEECCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCCHHHH
SNFDVGLDLDEEGYRILSSKIIVEEVESEDHQISLIEVKQVADDAQIGDTVVLDVTPEKE
CCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEHHHHHCCCCCCCEEEEEECCCHH
DFGRMAASTTKQVLAQKLRDQQRKMIQEEFADLEDPVLTARVIRFERQSVIMGVSSGIGR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHEEEHHCCCCC
PEVEAELPKRDQLPNDNYRANATFKVFLKEVSEIARKGPQLFVSRANAGLVVYLFENEVP
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEECCCCC
EIQEGTVKIVAVSREANPPSRAVGPRTKVAVDSVENEVDPVGACIGARGARIQQVVNELR
CCCCCCEEEEEEECCCCCCCCCCCCCCCEEHHHHHCCCCHHHHHHCCCCHHHHHHHHHHC
GEKIDVIKWSSDPIQYILNSLSPAKVDLVRLVDPEGQHAHVLVPPDQLSLAIGREGQNVR
CCEEEEEEECCCHHHHHHHCCCCCCEEEEEEECCCCCEEEEEECCCCEEEEECCCCCCEE
LAARLTGWKIDVKNSHEYDQEAEDAAVSELIIQREDEEKLQREAELRLEAEQAERAAEDA
EEEEEECEEEEECCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHH
RLRELYPLPEDEEEYGEEQYEGEELTDNDPLETLQDTDISAKEEKKR
HHHHHCCCCCCHHHHCHHHCCCCCCCCCCCHHHHHCCCCCCHHHCCC
>Mature Secondary Structure 
ALVILPGLNNLIEDISEEKKLPPNIVEAALREALLKGYEKYRRTFYIGVNEDPFDEEYF
EEEEECCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCCHHHH
SNFDVGLDLDEEGYRILSSKIIVEEVESEDHQISLIEVKQVADDAQIGDTVVLDVTPEKE
CCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEHHHHHCCCCCCCEEEEEECCCHH
DFGRMAASTTKQVLAQKLRDQQRKMIQEEFADLEDPVLTARVIRFERQSVIMGVSSGIGR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHEEEHHCCCCC
PEVEAELPKRDQLPNDNYRANATFKVFLKEVSEIARKGPQLFVSRANAGLVVYLFENEVP
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEECCCCC
EIQEGTVKIVAVSREANPPSRAVGPRTKVAVDSVENEVDPVGACIGARGARIQQVVNELR
CCCCCCEEEEEEECCCCCCCCCCCCCCCEEHHHHHCCCCHHHHHHCCCCHHHHHHHHHHC
GEKIDVIKWSSDPIQYILNSLSPAKVDLVRLVDPEGQHAHVLVPPDQLSLAIGREGQNVR
CCEEEEEEECCCHHHHHHHCCCCCCEEEEEEECCCCCEEEEEECCCCEEEEECCCCCCEE
LAARLTGWKIDVKNSHEYDQEAEDAAVSELIIQREDEEKLQREAELRLEAEQAERAAEDA
EEEEEECEEEEECCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHH
RLRELYPLPEDEEEYGEEQYEGEELTDNDPLETLQDTDISAKEEKKR
HHHHHCCCCCCHHHHCHHHCCCCCCCCCCCHHHHHCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]