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The map label for this gene is truB

Identifier: 126696871

GI number: 126696871

Start: 1300173

End: 1301090

Strand: Direct

Name: truB

Synonym: P9301_15331

Alternate gene names: 126696871

Gene position: 1300173-1301090 (Clockwise)

Preceding gene: 126696869

Following gene: 126696873

Centisome position: 79.19

GC content: 30.39

Gene sequence:

>918_bases
ATGGAAACTAAAGATGGATTCTTAGTAATTAATAAAGATAAAGGATGTACTTCACATGATTGTGTTAAACAAATAAGGAA
GTTACTAAATACAAAAAAGGTCGGTCACACAGGAACTCTTGACCCAGAAGTTATAGGAATATTACCAATCGCGATAGGCA
GTGCAACAAGATTTATTCAATATCTACCTCAGGGTAAAACTTACATAGGACAAATTAAATTAGGGATAAGAACTAACACT
GATGATATTCACGGAGAAATAATTAATCAAAAAAGTTGGCCTAAAATCAGTGATGAAAAATTAGATCAATACTTAAATAG
ATTTAGGGGAATTATTAAACAAATTCCGCCGAAAGTATCTAGTGTGCACATCAATGGTGAGAGGGCTTATAAAAAATCTT
TCAGGAATGAAGTTTTTGAATTAGCACCAAGAGAAGTAAAAATAGACGAACTTACTTTAATGAAATGGGACCAAATAAAC
GGAATATTAGAAATAAAAGTTAAATGTTCAGCTGGCACATACATAAGAGCAATTGCAAGAGATTTAGGAGAAATTCTTAA
TTCCGAGGGTTGCCTTCTACAGCTAAAAAGAATTTCAGCTTGTGGCTTTGATGAACAAAACTCGATAAAAATATCTGATA
TCGAAAAAGATAAAAAAAACTCGAAAAATTTCATTATTCCAACAATTTCTGCTCTTAATCACATTTCATCATTTGTCTTA
AGTACCGAAGAACAAATCAATTTTTGGCAAACAGGAAGAGCAATTAGAGTTGATATTAATTACTTTCAGGAAAATAAATC
TTTTGACTACAAAAAACCCATAAAAGTAATAGATAAAAAACAAATACTGCTTGGGATAGGTTTCTTAAATGAAGAGCAAT
CTAATATAAATCCAAAATTAGTCCTTAATGCTAAATAA

Upstream 100 bases:

>100_bases
TAAAATAAAATTATTGTTTTTCAATTTCTTTCTCTAAATTTCCAATATCAAAATCATAGGATGGTTATAAATCTTTCTCA
AAGTATTCTTAATATTAAAA

Downstream 100 bases:

>100_bases
CTTCTATAGGTAATCTTTTCTAAAAGGTTTAACCTGCACACCCTTATAAAAATGCTTTAATATTCTTTCAGCTTTTTGGC
CTCTAGACGCCAGATATTTA

Product: putative tRNA pseudouridine 55 synthase

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase

Number of amino acids: Translated: 305; Mature: 305

Protein sequence:

>305_residues
METKDGFLVINKDKGCTSHDCVKQIRKLLNTKKVGHTGTLDPEVIGILPIAIGSATRFIQYLPQGKTYIGQIKLGIRTNT
DDIHGEIINQKSWPKISDEKLDQYLNRFRGIIKQIPPKVSSVHINGERAYKKSFRNEVFELAPREVKIDELTLMKWDQIN
GILEIKVKCSAGTYIRAIARDLGEILNSEGCLLQLKRISACGFDEQNSIKISDIEKDKKNSKNFIIPTISALNHISSFVL
STEEQINFWQTGRAIRVDINYFQENKSFDYKKPIKVIDKKQILLGIGFLNEEQSNINPKLVLNAK

Sequences:

>Translated_305_residues
METKDGFLVINKDKGCTSHDCVKQIRKLLNTKKVGHTGTLDPEVIGILPIAIGSATRFIQYLPQGKTYIGQIKLGIRTNT
DDIHGEIINQKSWPKISDEKLDQYLNRFRGIIKQIPPKVSSVHINGERAYKKSFRNEVFELAPREVKIDELTLMKWDQIN
GILEIKVKCSAGTYIRAIARDLGEILNSEGCLLQLKRISACGFDEQNSIKISDIEKDKKNSKNFIIPTISALNHISSFVL
STEEQINFWQTGRAIRVDINYFQENKSFDYKKPIKVIDKKQILLGIGFLNEEQSNINPKLVLNAK
>Mature_305_residues
METKDGFLVINKDKGCTSHDCVKQIRKLLNTKKVGHTGTLDPEVIGILPIAIGSATRFIQYLPQGKTYIGQIKLGIRTNT
DDIHGEIINQKSWPKISDEKLDQYLNRFRGIIKQIPPKVSSVHINGERAYKKSFRNEVFELAPREVKIDELTLMKWDQIN
GILEIKVKCSAGTYIRAIARDLGEILNSEGCLLQLKRISACGFDEQNSIKISDIEKDKKNSKNFIIPTISALNHISSFVL
STEEQINFWQTGRAIRVDINYFQENKSFDYKKPIKVIDKKQILLGIGFLNEEQSNINPKLVLNAK

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI21040257, Length=210, Percent_Identity=32.3809523809524, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI215599015, Length=207, Percent_Identity=28.0193236714976, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI4503337, Length=207, Percent_Identity=28.0193236714976, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI2367200, Length=232, Percent_Identity=31.0344827586207, Blast_Score=129, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17553978, Length=211, Percent_Identity=29.8578199052133, Blast_Score=76, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6323204, Length=211, Percent_Identity=27.0142180094787, Blast_Score=75, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6324037, Length=132, Percent_Identity=32.5757575757576, Blast_Score=66, Evalue=6e-12,
Organism=Drosophila melanogaster, GI281364189, Length=196, Percent_Identity=31.1224489795918, Blast_Score=88, Evalue=7e-18,
Organism=Drosophila melanogaster, GI281364187, Length=196, Percent_Identity=31.1224489795918, Blast_Score=88, Evalue=7e-18,
Organism=Drosophila melanogaster, GI281364185, Length=196, Percent_Identity=31.1224489795918, Blast_Score=88, Evalue=7e-18,
Organism=Drosophila melanogaster, GI281364183, Length=196, Percent_Identity=31.1224489795918, Blast_Score=88, Evalue=7e-18,
Organism=Drosophila melanogaster, GI62471759, Length=196, Percent_Identity=31.1224489795918, Blast_Score=88, Evalue=7e-18,
Organism=Drosophila melanogaster, GI17975520, Length=196, Percent_Identity=31.1224489795918, Blast_Score=88, Evalue=7e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUB_PROM0 (A3PEI1)

Other databases:

- EMBL:   CP000576
- RefSeq:   YP_001091757.1
- ProteinModelPortal:   A3PEI1
- SMR:   A3PEI1
- STRING:   A3PEI1
- GeneID:   4912420
- GenomeReviews:   CP000576_GR
- KEGG:   pmg:P9301_15331
- eggNOG:   COG0130
- HOGENOM:   HBG397258
- OMA:   ASTKAYD
- ProtClustDB:   CLSK2516628
- BioCyc:   PMAR167546:P9301ORF_1562-MONOMER
- HAMAP:   MF_01080
- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR014780
- TIGRFAMs:   TIGR00431

Pfam domain/function: PF01509 TruB_N; SSF55120 PsdUridine_synth_cat_dom

EC number: 4.2.1.70

Molecular weight: Translated: 34673; Mature: 34673

Theoretical pI: Translated: 9.79; Mature: 9.79

Prosite motif: NA

Important sites: ACT_SITE 41-41

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METKDGFLVINKDKGCTSHDCVKQIRKLLNTKKVGHTGTLDPEVIGILPIAIGSATRFIQ
CCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHEEEEEEHHCHHHHHHH
YLPQGKTYIGQIKLGIRTNTDDIHGEIINQKSWPKISDEKLDQYLNRFRGIIKQIPPKVS
HCCCCCEEEEEEEEEEECCCCHHHHEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCE
SVHINGERAYKKSFRNEVFELAPREVKIDELTLMKWDQINGILEIKVKCSAGTYIRAIAR
EEEECCHHHHHHHHHHHHHHCCCCEEEECCEEEEEHHCCCCEEEEEEEECCCHHHHHHHH
DLGEILNSEGCLLQLKRISACGFDEQNSIKISDIEKDKKNSKNFIIPTISALNHISSFVL
HHHHHHCCCCCEEEEEHHHHCCCCCCCCEEEEECCCCCCCCCCEEEEHHHHHHHHHHHHH
STEEQINFWQTGRAIRVDINYFQENKSFDYKKPIKVIDKKQILLGIGFLNEEQSNINPKL
CCHHHCCHHHCCCEEEEEEEEEECCCCCCCCCCHHHHCCCEEEEEEEECCCCCCCCCCEE
VLNAK
EEECC
>Mature Secondary Structure
METKDGFLVINKDKGCTSHDCVKQIRKLLNTKKVGHTGTLDPEVIGILPIAIGSATRFIQ
CCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHEEEEEEHHCHHHHHHH
YLPQGKTYIGQIKLGIRTNTDDIHGEIINQKSWPKISDEKLDQYLNRFRGIIKQIPPKVS
HCCCCCEEEEEEEEEEECCCCHHHHEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCE
SVHINGERAYKKSFRNEVFELAPREVKIDELTLMKWDQINGILEIKVKCSAGTYIRAIAR
EEEECCHHHHHHHHHHHHHHCCCCEEEECCEEEEEHHCCCCEEEEEEEECCCHHHHHHHH
DLGEILNSEGCLLQLKRISACGFDEQNSIKISDIEKDKKNSKNFIIPTISALNHISSFVL
HHHHHHCCCCCEEEEEHHHHCCCCCCCCEEEEECCCCCCCCCCEEEEHHHHHHHHHHHHH
STEEQINFWQTGRAIRVDINYFQENKSFDYKKPIKVIDKKQILLGIGFLNEEQSNINPKL
CCHHHCCHHHCCCEEEEEEEEEECCCCCCCCCCHHHHCCCEEEEEEEECCCCCCCCCCEE
VLNAK
EEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA