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The map label for this gene is ribB [H]

Identifier: 126696304

GI number: 126696304

Start: 832549

End: 834273

Strand: Reverse

Name: ribB [H]

Synonym: P9301_09661

Alternate gene names: 126696304

Gene position: 834273-832549 (Counterclockwise)

Preceding gene: 126696306

Following gene: 126696299

Centisome position: 50.81

GC content: 32.7

Gene sequence:

>1725_bases
ATGAAAGAAACAAGTCCCAAATCAAATAATGGAACAATTTTGGATATAAATGATTCTTTTAAAATTGAATTTGACCCTAT
CAGCGATGCATTGGCAGCTATAAGAAATGGGGAATGCATAATTGTTGTAGATGATGAGAGAAGAGAAAATGAAGGAGATT
TAATATGTGCGGCTCAGTTTGCAACTCCACAGCAAATTAATTTTATGGCTACCGAAGGACGTGGACTTATATGCCTAGCC
ATGCAAGGTGAAAAACTTGACTCTTTAGATTTACCATTAATGGTAGATAGAAATACAGATGAAAATCAAACAGCTTTTAC
GATATCAATTGATGCTGGACCTGAAAATAATGTTACTACTGGAATTTCTGCTGAAGACAGGGCAAAGACAATTCAAGTTG
CTATAAACCCAAATACAAAACCCGATGATTTGAGAAGGCCAGGACATATTTTTCCATTAAGAGCTAAGAAAGGTGGAGTA
TTAAAAAGAGCAGGTCATACTGAAGCGGCAGTAGATATTGCGGCAATGTCAGGTCTTTATCCCGCTGGAGTGATTTGCGA
AATACAAAATCCTGATGGTTCCATGTCTAGACTTCCACAACTTAAAGAGTATGCAAAACAGTGGGGAATGAAATTAATAT
CCATAGCCGATTTAATTAGTTATCGTTTTCAAACTGAGAGATTTGTATTTAGAAAATCTGATGCGGTACTTCCAAGTATT
TTTGGTAATTTCAAAGCTTATGGATACGTTAATGAACTTGATGGTTCAGAGCACGTTGCATTAGTTAAACAAAAATCATC
CAAATTAAGAGAGCCTGTACTTGTAAGAATGCATTCAGAGTGCTTAACTGGCGATGCTTTTGGATCTTTACGTTGTGATT
GTAGACCTCAGTTAGAGGCTGCTTTATCAAGGATTGAAAAGGAAGAAGAAGGAGTTGTTGTTTACTTGAGACAAGAAGGT
AGAGGTATTGGTCTTATAAATAAATTAAAGGCTTATAGTTTACAGGATGGTGGATTAGACACCGTAGAAGCTAATGAAAA
ATTAGGTTTTCCAGCTGATCTAAGAAATTATGGAGTTGGAGCACAGATATTAACCGATCTAGGGATAAAAAAACTAAAAT
TACTTACAAACAATCCTAGAAAGATTGCTGGATTAGGTGGTTATGGAATAGAAGTTATTGAGAGAGTTCCATTGGTTATT
TGTCCAAACGATAATAATGCAGAATATTTGAGTGTAAAAAAAACCAAGTTAGGCCATATGATTGATGAAGAAAATTCTAA
TTCAAGGAATATCGATCCATTCATATCAATTTTTCTTGACGGTAAATATAAATCTAATGATCTAGTTCCAATAAAAAATG
CCGTTATTAAATTCTGTAGTTATAAGAACATTAATATTAAACTAGAAAGTACGCCAAGATTATTGGCTTTTTGGAATCGA
CCAAAATTAGTTTGGAGAATTTTGCATGATCAGAACAGAACTAATTCCAACATTACTGATGAAGAAATAAAGAATATAGA
ATTATTTATTCAGTTTTTATCTAATTATGAAAATAGTACAAAGATTGGAATTATAGTTTCTAGAAACATTGAACAGGCTT
TACACCCAACAAATAGCATCAAATTAATCAATACAAAATTTACTATTAATAATGAAATTTTATATTCATCTACAAGAAAA
TTTAATCTAGATAAAGAGACGTTTAGTATTGTTTTTGAAGGCTAA

Upstream 100 bases:

>100_bases
TGCAACATTCATTTCTTTGAATTGGCAGACTTTGAGATAGGATTAATAAGTGTTGAATTTAGGGTAACTAAAACACTATT
TTTATATATTGATAGGAATA

Downstream 100 bases:

>100_bases
GTTACTATTTTAAAGTTGCTTTTAGAATTTTTGAACCATTAGTAAGCTTTAGAACTACATCCATATCATCAGTCTTACCA
AAAACAGTATGAACTCCATC

Product: bifunctional 3,4-dihydroxy-2-butanone 4-phosphate synthase/GTP cyclohydrolase II/unknown domain fusion protein

Products: NA

Alternate protein names: 3,4-dihydroxy-2-butanone 4-phosphate synthase; DHBP synthase; GTP cyclohydrolase-2; GTP cyclohydrolase II [H]

Number of amino acids: Translated: 574; Mature: 574

Protein sequence:

>574_residues
MKETSPKSNNGTILDINDSFKIEFDPISDALAAIRNGECIIVVDDERRENEGDLICAAQFATPQQINFMATEGRGLICLA
MQGEKLDSLDLPLMVDRNTDENQTAFTISIDAGPENNVTTGISAEDRAKTIQVAINPNTKPDDLRRPGHIFPLRAKKGGV
LKRAGHTEAAVDIAAMSGLYPAGVICEIQNPDGSMSRLPQLKEYAKQWGMKLISIADLISYRFQTERFVFRKSDAVLPSI
FGNFKAYGYVNELDGSEHVALVKQKSSKLREPVLVRMHSECLTGDAFGSLRCDCRPQLEAALSRIEKEEEGVVVYLRQEG
RGIGLINKLKAYSLQDGGLDTVEANEKLGFPADLRNYGVGAQILTDLGIKKLKLLTNNPRKIAGLGGYGIEVIERVPLVI
CPNDNNAEYLSVKKTKLGHMIDEENSNSRNIDPFISIFLDGKYKSNDLVPIKNAVIKFCSYKNINIKLESTPRLLAFWNR
PKLVWRILHDQNRTNSNITDEEIKNIELFIQFLSNYENSTKIGIIVSRNIEQALHPTNSIKLINTKFTINNEILYSSTRK
FNLDKETFSIVFEG

Sequences:

>Translated_574_residues
MKETSPKSNNGTILDINDSFKIEFDPISDALAAIRNGECIIVVDDERRENEGDLICAAQFATPQQINFMATEGRGLICLA
MQGEKLDSLDLPLMVDRNTDENQTAFTISIDAGPENNVTTGISAEDRAKTIQVAINPNTKPDDLRRPGHIFPLRAKKGGV
LKRAGHTEAAVDIAAMSGLYPAGVICEIQNPDGSMSRLPQLKEYAKQWGMKLISIADLISYRFQTERFVFRKSDAVLPSI
FGNFKAYGYVNELDGSEHVALVKQKSSKLREPVLVRMHSECLTGDAFGSLRCDCRPQLEAALSRIEKEEEGVVVYLRQEG
RGIGLINKLKAYSLQDGGLDTVEANEKLGFPADLRNYGVGAQILTDLGIKKLKLLTNNPRKIAGLGGYGIEVIERVPLVI
CPNDNNAEYLSVKKTKLGHMIDEENSNSRNIDPFISIFLDGKYKSNDLVPIKNAVIKFCSYKNINIKLESTPRLLAFWNR
PKLVWRILHDQNRTNSNITDEEIKNIELFIQFLSNYENSTKIGIIVSRNIEQALHPTNSIKLINTKFTINNEILYSSTRK
FNLDKETFSIVFEG
>Mature_574_residues
MKETSPKSNNGTILDINDSFKIEFDPISDALAAIRNGECIIVVDDERRENEGDLICAAQFATPQQINFMATEGRGLICLA
MQGEKLDSLDLPLMVDRNTDENQTAFTISIDAGPENNVTTGISAEDRAKTIQVAINPNTKPDDLRRPGHIFPLRAKKGGV
LKRAGHTEAAVDIAAMSGLYPAGVICEIQNPDGSMSRLPQLKEYAKQWGMKLISIADLISYRFQTERFVFRKSDAVLPSI
FGNFKAYGYVNELDGSEHVALVKQKSSKLREPVLVRMHSECLTGDAFGSLRCDCRPQLEAALSRIEKEEEGVVVYLRQEG
RGIGLINKLKAYSLQDGGLDTVEANEKLGFPADLRNYGVGAQILTDLGIKKLKLLTNNPRKIAGLGGYGIEVIERVPLVI
CPNDNNAEYLSVKKTKLGHMIDEENSNSRNIDPFISIFLDGKYKSNDLVPIKNAVIKFCSYKNINIKLESTPRLLAFWNR
PKLVWRILHDQNRTNSNITDEEIKNIELFIQFLSNYENSTKIGIIVSRNIEQALHPTNSIKLINTKFTINNEILYSSTRK
FNLDKETFSIVFEG

Specific function: Catalyzes the conversion of GTP to 2,5-diamino-6- ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate [H]

COG id: COG0108

COG function: function code H; 3,4-dihydroxy-2-butanone 4-phosphate synthase

Gene ontology:

Cell location: Membrane-Associated [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the central section; belongs to the GTP cyclohydrolase II family [H]

Homologues:

Organism=Escherichia coli, GI1789420, Length=209, Percent_Identity=47.3684210526316, Blast_Score=214, Evalue=2e-56,
Organism=Escherichia coli, GI1787533, Length=193, Percent_Identity=50.7772020725389, Blast_Score=197, Evalue=1e-51,
Organism=Saccharomyces cerevisiae, GI6320695, Length=206, Percent_Identity=42.2330097087379, Blast_Score=163, Evalue=6e-41,
Organism=Saccharomyces cerevisiae, GI6319438, Length=172, Percent_Identity=45.3488372093023, Blast_Score=133, Evalue=1e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017945
- InterPro:   IPR000422
- InterPro:   IPR000926
- InterPro:   IPR016299 [H]

Pfam domain/function: PF00926 DHBP_synthase; PF00925 GTP_cyclohydro2 [H]

EC number: =4.1.99.12; =3.5.4.25 [H]

Molecular weight: Translated: 64092; Mature: 64092

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKETSPKSNNGTILDINDSFKIEFDPISDALAAIRNGECIIVVDDERRENEGDLICAAQF
CCCCCCCCCCCEEEEECCCEEEEECCHHHHHHHHCCCCEEEEECCCCCCCCCCEEEEEEC
ATPQQINFMATEGRGLICLAMQGEKLDSLDLPLMVDRNTDENQTAFTISIDAGPENNVTT
CCCCEEEEEEECCCCEEEEEECCCCCCCCCCCEEEECCCCCCCEEEEEEEECCCCCCCCC
GISAEDRAKTIQVAINPNTKPDDLRRPGHIFPLRAKKGGVLKRAGHTEAAVDIAAMSGLY
CCCCCCCCEEEEEEECCCCCHHHHCCCCCEEEEECCCCCCEEECCCCHHHEEHHHHCCCC
PAGVICEIQNPDGSMSRLPQLKEYAKQWGMKLISIADLISYRFQTERFVFRKSDAVLPSI
CCCEEEEEECCCCHHHHCHHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEEECCCCCHHHH
FGNFKAYGYVNELDGSEHVALVKQKSSKLREPVLVRMHSECLTGDAFGSLRCDCRPQLEA
HCCCEEEEEEECCCCCCEEEEEEHHHHHCCCCEEEEEHHHHCCCCCCCCEEECCCHHHHH
ALSRIEKEEEGVVVYLRQEGRGIGLINKLKAYSLQDGGLDTVEANEKLGFPADLRNYGVG
HHHHHCCCCCCEEEEEEECCCCCCCHHHHEEEEECCCCCCEEECCCCCCCCHHHHHCCCC
AQILTDLGIKKLKLLTNNPRKIAGLGGYGIEVIERVPLVICPNDNNAEYLSVKKTKLGHM
HHHHHHCCCEEEEEEECCCCEEEECCCCHHHHHHHCCEEEECCCCCCEEEEEEHHHHCCC
IDEENSNSRNIDPFISIFLDGKYKSNDLVPIKNAVIKFCSYKNINIKLESTPRLLAFWNR
CCCCCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHCCCCEEEEECCCCCEEEEECC
PKLVWRILHDQNRTNSNITDEEIKNIELFIQFLSNYENSTKIGIIVSRNIEQALHPTNSI
CCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEECCHHHHHCCCCCE
KLINTKFTINNEILYSSTRKFNLDKETFSIVFEG
EEEEEEEEECCEEEEECCCEECCCCEEEEEEEEC
>Mature Secondary Structure
MKETSPKSNNGTILDINDSFKIEFDPISDALAAIRNGECIIVVDDERRENEGDLICAAQF
CCCCCCCCCCCEEEEECCCEEEEECCHHHHHHHHCCCCEEEEECCCCCCCCCCEEEEEEC
ATPQQINFMATEGRGLICLAMQGEKLDSLDLPLMVDRNTDENQTAFTISIDAGPENNVTT
CCCCEEEEEEECCCCEEEEEECCCCCCCCCCCEEEECCCCCCCEEEEEEEECCCCCCCCC
GISAEDRAKTIQVAINPNTKPDDLRRPGHIFPLRAKKGGVLKRAGHTEAAVDIAAMSGLY
CCCCCCCCEEEEEEECCCCCHHHHCCCCCEEEEECCCCCCEEECCCCHHHEEHHHHCCCC
PAGVICEIQNPDGSMSRLPQLKEYAKQWGMKLISIADLISYRFQTERFVFRKSDAVLPSI
CCCEEEEEECCCCHHHHCHHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEEECCCCCHHHH
FGNFKAYGYVNELDGSEHVALVKQKSSKLREPVLVRMHSECLTGDAFGSLRCDCRPQLEA
HCCCEEEEEEECCCCCCEEEEEEHHHHHCCCCEEEEEHHHHCCCCCCCCEEECCCHHHHH
ALSRIEKEEEGVVVYLRQEGRGIGLINKLKAYSLQDGGLDTVEANEKLGFPADLRNYGVG
HHHHHCCCCCCEEEEEEECCCCCCCHHHHEEEEECCCCCCEEECCCCCCCCHHHHHCCCC
AQILTDLGIKKLKLLTNNPRKIAGLGGYGIEVIERVPLVICPNDNNAEYLSVKKTKLGHM
HHHHHHCCCEEEEEEECCCCEEEECCCCHHHHHHHCCEEEECCCCCCEEEEEEHHHHCCC
IDEENSNSRNIDPFISIFLDGKYKSNDLVPIKNAVIKFCSYKNINIKLESTPRLLAFWNR
CCCCCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHCCCCEEEEECCCCCEEEEECC
PKLVWRILHDQNRTNSNITDEEIKNIELFIQFLSNYENSTKIGIIVSRNIEQALHPTNSI
CCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEECCHHHHHCCCCCE
KLINTKFTINNEILYSSTRKFNLDKETFSIVFEG
EEEEEEEEECCEEEEECCCEECCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]