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The map label for this gene is purN [H]

Identifier: 126696306

GI number: 126696306

Start: 835401

End: 836057

Strand: Reverse

Name: purN [H]

Synonym: P9301_09681

Alternate gene names: 126696306

Gene position: 836057-835401 (Counterclockwise)

Preceding gene: 126696308

Following gene: 126696304

Centisome position: 50.92

GC content: 30.44

Gene sequence:

>657_bases
TTGGATAGATCATTTAATTACATAATTTCCCCTGAGATATCTGAATTTAGAAGATTTTCCCCAAAATTAAAAATCGGTGT
ACTTGCTTCTGGGAAAGGAACAAACTTTCAGGAATTAATTAATCTCTCAGAAAAAGGAGAATTAGATATAGATATAAGAG
TTCTAATAACTAACAAAGATGATGCAGGTTGTATAAAGAGAGCTGAAAGTAAAAAAATACCTCACAAAATTATAAGAGGC
AAAGACTTTCTGCAAAAAGAGGCATTTGAATTAGAAATTGTGAATACTTTAATTCATTACGATGTCGAACTTGTAGTTAT
GGCAGGCTGGATGAAAATTGTTACTCCATTTTTTATTAATAAATTTAAGAATAAGATAATAAATATTCACCCTTCATTAC
TCCCATCATATAAAGGTGGTTCTGCGATAAAGGACTCTATATTGAATGGTTCAAAAATAACTGGTTGTTCCGTACATTTT
GTTGAAGAGGAGGTAGATAGTGGATCACTGATTATGCAAGCTGCATTGTCTATTAGAAATGATGATGATATTGAATCACT
TTCCAAAAGAATACAAATGCTTGAACATAAAATTTTACCTCACTCAATCTCTCTTGCTGGTTTTTTGATAAGAAGTAACT
TTATGGAAAATTATTAG

Upstream 100 bases:

>100_bases
AATTATTATCTTCAAATAAATATGTTTTTGAATATAAACAATTGAAATTAAGGGTTCTTTTTATTTCATAAGAATTTATC
ATTGGATAAAATAAAAAACT

Downstream 100 bases:

>100_bases
TTAAATCAAGACCTTCATCCATTGAAAATCCACTCATAATATTTAAATTTTGAATTGCCTGCCCAGTCTGACCTTTTAAC
AAATTATCAATTACAGACAA

Product: phosphoribosylglycinamide formyltransferase

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 218; Mature: 218

Protein sequence:

>218_residues
MDRSFNYIISPEISEFRRFSPKLKIGVLASGKGTNFQELINLSEKGELDIDIRVLITNKDDAGCIKRAESKKIPHKIIRG
KDFLQKEAFELEIVNTLIHYDVELVVMAGWMKIVTPFFINKFKNKIINIHPSLLPSYKGGSAIKDSILNGSKITGCSVHF
VEEEVDSGSLIMQAALSIRNDDDIESLSKRIQMLEHKILPHSISLAGFLIRSNFMENY

Sequences:

>Translated_218_residues
MDRSFNYIISPEISEFRRFSPKLKIGVLASGKGTNFQELINLSEKGELDIDIRVLITNKDDAGCIKRAESKKIPHKIIRG
KDFLQKEAFELEIVNTLIHYDVELVVMAGWMKIVTPFFINKFKNKIINIHPSLLPSYKGGSAIKDSILNGSKITGCSVHF
VEEEVDSGSLIMQAALSIRNDDDIESLSKRIQMLEHKILPHSISLAGFLIRSNFMENY
>Mature_218_residues
MDRSFNYIISPEISEFRRFSPKLKIGVLASGKGTNFQELINLSEKGELDIDIRVLITNKDDAGCIKRAESKKIPHKIIRG
KDFLQKEAFELEIVNTLIHYDVELVVMAGWMKIVTPFFINKFKNKIINIHPSLLPSYKGGSAIKDSILNGSKITGCSVHF
VEEEVDSGSLIMQAALSIRNDDDIESLSKRIQMLEHKILPHSISLAGFLIRSNFMENY

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=184, Percent_Identity=36.9565217391304, Blast_Score=149, Evalue=1e-36,
Organism=Homo sapiens, GI209869995, Length=184, Percent_Identity=36.9565217391304, Blast_Score=149, Evalue=1e-36,
Organism=Homo sapiens, GI209869993, Length=184, Percent_Identity=36.9565217391304, Blast_Score=149, Evalue=1e-36,
Organism=Escherichia coli, GI1788846, Length=182, Percent_Identity=34.6153846153846, Blast_Score=130, Evalue=7e-32,
Organism=Escherichia coli, GI1787483, Length=195, Percent_Identity=27.1794871794872, Blast_Score=87, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI17567511, Length=182, Percent_Identity=36.8131868131868, Blast_Score=147, Evalue=4e-36,
Organism=Saccharomyces cerevisiae, GI6320616, Length=196, Percent_Identity=27.5510204081633, Blast_Score=72, Evalue=7e-14,
Organism=Drosophila melanogaster, GI24582400, Length=184, Percent_Identity=37.5, Blast_Score=144, Evalue=4e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 24669; Mature: 24669

Theoretical pI: Translated: 8.23; Mature: 8.23

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRSFNYIISPEISEFRRFSPKLKIGVLASGKGTNFQELINLSEKGELDIDIRVLITNKD
CCCCCCEEECCCHHHHHHCCCCEEEEEEECCCCCCHHHHHCCCCCCCCEEEEEEEEECCC
DAGCIKRAESKKIPHKIIRGKDFLQKEAFELEIVNTLIHYDVELVVMAGWMKIVTPFFIN
CHHHHHHHHHCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHH
KFKNKIINIHPSLLPSYKGGSAIKDSILNGSKITGCSVHFVEEEVDSGSLIMQAALSIRN
HHHHHEEEECHHHCCCCCCCCHHHHHHCCCCCCCCEEEEEEHHHCCCCHHHHHHHHHCCC
DDDIESLSKRIQMLEHKILPHSISLAGFLIRSNFMENY
CCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDRSFNYIISPEISEFRRFSPKLKIGVLASGKGTNFQELINLSEKGELDIDIRVLITNKD
CCCCCCEEECCCHHHHHHCCCCEEEEEEECCCCCCHHHHHCCCCCCCCEEEEEEEEECCC
DAGCIKRAESKKIPHKIIRGKDFLQKEAFELEIVNTLIHYDVELVVMAGWMKIVTPFFIN
CHHHHHHHHHCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHH
KFKNKIINIHPSLLPSYKGGSAIKDSILNGSKITGCSVHFVEEEVDSGSLIMQAALSIRN
HHHHHEEEECHHHCCCCCCCCHHHHHHCCCCCCCCEEEEEEHHHCCCCHHHHHHHHHCCC
DDDIESLSKRIQMLEHKILPHSISLAGFLIRSNFMENY
CCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA