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The map label for this gene is metB [H]

Identifier: 126695766

GI number: 126695766

Start: 374357

End: 375520

Strand: Reverse

Name: metB [H]

Synonym: P9301_04281

Alternate gene names: 126695766

Gene position: 375520-374357 (Counterclockwise)

Preceding gene: 126695767

Following gene: 126695765

Centisome position: 22.87

GC content: 33.85

Gene sequence:

>1164_bases
ATGGGAAATAAGGAAAATAATATAGAAAAGCCAGGTGTTAAGACCTTATCTATTCACCATGGGAAAACATTTGCAGAAGA
AACTGGATGCGTTATGCCTCCTATTTTTTCTACATCTACTTTCAAACATGGAAATAAAGATAATTTCGACTACACAAGAT
CAGGCAATCCAAACTTTAGAATTCTAGAAAACATCCTTAAATTAATAGAAGAATCTAAATACTGTACAGTTTTTGGATCT
GGAATTAGCGCGGTAACTGCAATTTCATCAACACTGAAATCAGGTGACAAGGTACTCTGCGAGTCAAATCTCTATGGTTG
TACAGTGAGGATGTTCGAAAAAGTTTTCAAGAAATTTGGACTAGAAGTTTTATACACAGATTTTACAAACGAAAATAACG
TCAAAAAGATTTCAAAATTGGAGCCAAACTTGATATGGCTAGAAAGTCCAACTAATCCACTTTTGAAGGTACTCGATATT
AAGGCGATTTGTGATGAAGCGAATAAACTCGAAATACCAGTAGTTGTAGACAACACATTTTCTACAGCTCTTATTCAAAA
ACCATTGGACCTTGGTGCAACACTATCGCTTGTAAGCACCACAAAATTCATTAATGGACATAGTGACGCACTTGGCGGAG
CAGTACTTACAAATAATGAGGAATGGAATAGTAAGATGCTTTTCTCTCAAAAAGCTCTAGGGCTTCAACCATCTCCTTTT
GATAGTTGGCTCATTACGAGAGGAGTAAAAACTCTTCCTTTAAGAATCGAACAACAAACTAAAAGTGCAGAATTTATTTC
TGAAGAATTAGGTAATCATAAAATTATTAGTAAAGTAATTTACCCTTTTAATCAAGAACATCCGCAATTTAATTTAGCAA
AATCGCAAATGAAATCTGGAGGTTCAATGATCACCCTAAAATTAAACTTAAATAAAGAGGATACTTTTAAATTTTGCAAA
TCTCTCAAATATTTCTCTCTAGCAGAAAGCCTTGGAGGAGTTGAAAGTTTAATTTGTCACCCTGCAACGATGACTCATGC
TTCTGTTGATGACAAAACAAAAAATCTACTAGGGATAGATGATGCTCTTATCAGATTATCAATTGGATGTGAAGATACAG
ACGATTTAATCTCGGACATTTTATTTGCTTTAAATAAATTCTGA

Upstream 100 bases:

>100_bases
AATATTATTCAAGAAAAGTTTAATACTACTTTTCTTTGGATTATTAAATCTCTCTTTAAAAAAAAAGAGAGATTTAATTT
AATTCTTATTTTTAAAAATA

Downstream 100 bases:

>100_bases
AAATTGAGAGATTTACTTAAAAAACCTATATGGAAAAATTTAGAGTTGGGATATGCAATTCCTGATAGTATTCATGCTGT
TTCTGTAGCATTACCAACTT

Product: putative cystathionine gamma-synthase

Products: NA

Alternate protein names: CBL; Beta-cystathionase; Cysteine lyase [H]

Number of amino acids: Translated: 387; Mature: 386

Protein sequence:

>387_residues
MGNKENNIEKPGVKTLSIHHGKTFAEETGCVMPPIFSTSTFKHGNKDNFDYTRSGNPNFRILENILKLIEESKYCTVFGS
GISAVTAISSTLKSGDKVLCESNLYGCTVRMFEKVFKKFGLEVLYTDFTNENNVKKISKLEPNLIWLESPTNPLLKVLDI
KAICDEANKLEIPVVVDNTFSTALIQKPLDLGATLSLVSTTKFINGHSDALGGAVLTNNEEWNSKMLFSQKALGLQPSPF
DSWLITRGVKTLPLRIEQQTKSAEFISEELGNHKIISKVIYPFNQEHPQFNLAKSQMKSGGSMITLKLNLNKEDTFKFCK
SLKYFSLAESLGGVESLICHPATMTHASVDDKTKNLLGIDDALIRLSIGCEDTDDLISDILFALNKF

Sequences:

>Translated_387_residues
MGNKENNIEKPGVKTLSIHHGKTFAEETGCVMPPIFSTSTFKHGNKDNFDYTRSGNPNFRILENILKLIEESKYCTVFGS
GISAVTAISSTLKSGDKVLCESNLYGCTVRMFEKVFKKFGLEVLYTDFTNENNVKKISKLEPNLIWLESPTNPLLKVLDI
KAICDEANKLEIPVVVDNTFSTALIQKPLDLGATLSLVSTTKFINGHSDALGGAVLTNNEEWNSKMLFSQKALGLQPSPF
DSWLITRGVKTLPLRIEQQTKSAEFISEELGNHKIISKVIYPFNQEHPQFNLAKSQMKSGGSMITLKLNLNKEDTFKFCK
SLKYFSLAESLGGVESLICHPATMTHASVDDKTKNLLGIDDALIRLSIGCEDTDDLISDILFALNKF
>Mature_386_residues
GNKENNIEKPGVKTLSIHHGKTFAEETGCVMPPIFSTSTFKHGNKDNFDYTRSGNPNFRILENILKLIEESKYCTVFGSG
ISAVTAISSTLKSGDKVLCESNLYGCTVRMFEKVFKKFGLEVLYTDFTNENNVKKISKLEPNLIWLESPTNPLLKVLDIK
AICDEANKLEIPVVVDNTFSTALIQKPLDLGATLSLVSTTKFINGHSDALGGAVLTNNEEWNSKMLFSQKALGLQPSPFD
SWLITRGVKTLPLRIEQQTKSAEFISEELGNHKIISKVIYPFNQEHPQFNLAKSQMKSGGSMITLKLNLNKEDTFKFCKS
LKYFSLAESLGGVESLICHPATMTHASVDDKTKNLLGIDDALIRLSIGCEDTDDLISDILFALNKF

Specific function: The enzymatic degradation of amino acids in cheese is believed to generate aroma compounds and therefore to be essential for flavor development. Cystathionine beta-lyase (CBL) can convert cystathionine to homocysteine but is also able to catalyze an alpha

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=360, Percent_Identity=41.1111111111111, Blast_Score=275, Evalue=6e-74,
Organism=Homo sapiens, GI299473759, Length=360, Percent_Identity=37.7777777777778, Blast_Score=229, Evalue=4e-60,
Organism=Homo sapiens, GI25453487, Length=359, Percent_Identity=36.4902506963788, Blast_Score=223, Evalue=3e-58,
Organism=Escherichia coli, GI1790375, Length=370, Percent_Identity=35.4054054054054, Blast_Score=250, Evalue=1e-67,
Organism=Escherichia coli, GI1789383, Length=351, Percent_Identity=28.2051282051282, Blast_Score=131, Evalue=8e-32,
Organism=Caenorhabditis elegans, GI17538055, Length=368, Percent_Identity=42.3913043478261, Blast_Score=292, Evalue=2e-79,
Organism=Caenorhabditis elegans, GI17560056, Length=368, Percent_Identity=41.8478260869565, Blast_Score=290, Evalue=7e-79,
Organism=Caenorhabditis elegans, GI71980708, Length=408, Percent_Identity=32.3529411764706, Blast_Score=196, Evalue=1e-50,
Organism=Saccharomyces cerevisiae, GI6319307, Length=385, Percent_Identity=39.4805194805195, Blast_Score=268, Evalue=8e-73,
Organism=Saccharomyces cerevisiae, GI6321254, Length=389, Percent_Identity=30.3341902313625, Blast_Score=155, Evalue=8e-39,
Organism=Saccharomyces cerevisiae, GI6321904, Length=392, Percent_Identity=29.8469387755102, Blast_Score=135, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6323333, Length=404, Percent_Identity=29.9504950495049, Blast_Score=131, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6321170, Length=323, Percent_Identity=28.4829721362229, Blast_Score=126, Evalue=6e-30,
Organism=Drosophila melanogaster, GI19922554, Length=384, Percent_Identity=39.5833333333333, Blast_Score=287, Evalue=7e-78,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.8 [H]

Molecular weight: Translated: 42934; Mature: 42803

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGNKENNIEKPGVKTLSIHHGKTFAEETGCVMPPIFSTSTFKHGNKDNFDYTRSGNPNFR
CCCCCCCCCCCCCEEEEEECCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHH
ILENILKLIEESKYCTVFGSGISAVTAISSTLKSGDKVLCESNLYGCTVRMFEKVFKKFG
HHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHCC
LEVLYTDFTNENNVKKISKLEPNLIWLESPTNPLLKVLDIKAICDEANKLEIPVVVDNTF
CEEEEEECCCCCCHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHCCCCCEEEEEEECCCH
STALIQKPLDLGATLSLVSTTKFINGHSDALGGAVLTNNEEWNSKMLFSQKALGLQPSPF
HHHHHHCCCCCCCEEEHHHHHHHHCCCCCCCCCEEEECCCCCCCHHHHHHHHCCCCCCCC
DSWLITRGVKTLPLRIEQQTKSAEFISEELGNHKIISKVIYPFNQEHPQFNLAKSQMKSG
CHHHHHCCCEECCEEEECHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCHHHHHHHHCC
GSMITLKLNLNKEDTFKFCKSLKYFSLAESLGGVESLICHPATMTHASVDDKTKNLLGID
CCEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCHHCCCCCCCHHHHHCCCC
DALIRLSIGCEDTDDLISDILFALNKF
CEEEEEEECCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
GNKENNIEKPGVKTLSIHHGKTFAEETGCVMPPIFSTSTFKHGNKDNFDYTRSGNPNFR
CCCCCCCCCCCCEEEEEECCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHH
ILENILKLIEESKYCTVFGSGISAVTAISSTLKSGDKVLCESNLYGCTVRMFEKVFKKFG
HHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHCC
LEVLYTDFTNENNVKKISKLEPNLIWLESPTNPLLKVLDIKAICDEANKLEIPVVVDNTF
CEEEEEECCCCCCHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHCCCCCEEEEEEECCCH
STALIQKPLDLGATLSLVSTTKFINGHSDALGGAVLTNNEEWNSKMLFSQKALGLQPSPF
HHHHHHCCCCCCCEEEHHHHHHHHCCCCCCCCCEEEECCCCCCCHHHHHHHHCCCCCCCC
DSWLITRGVKTLPLRIEQQTKSAEFISEELGNHKIISKVIYPFNQEHPQFNLAKSQMKSG
CHHHHHCCCEECCEEEECHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCHHHHHHHHCC
GSMITLKLNLNKEDTFKFCKSLKYFSLAESLGGVESLICHPATMTHASVDDKTKNLLGID
CCEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCHHCCCCCCCHHHHHCCCC
DALIRLSIGCEDTDDLISDILFALNKF
CEEEEEEECCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11337471 [H]