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The map label for this gene is mdeA [H]

Identifier: 126695765

GI number: 126695765

Start: 372884

End: 374353

Strand: Reverse

Name: mdeA [H]

Synonym: P9301_04271

Alternate gene names: 126695765

Gene position: 374353-372884 (Counterclockwise)

Preceding gene: 126695766

Following gene: 126695764

Centisome position: 22.8

GC content: 29.52

Gene sequence:

>1470_bases
TTGAGAGATTTACTTAAAAAACCTATATGGAAAAATTTAGAGTTGGGATATGCAATTCCTGATAGTATTCATGCTGTTTC
TGTAGCATTACCAACTTGGAATGATGTAATAAATTACGAGGAAAAAGATCAAGAATGCATAAATTTATTGAAGTCCATTT
ACCCACGATTCGGGCTAAACCCCATAGTGAAAAGATTATGCGAAAAGGTAAAAAAACAAAATTACTACAACAATAAAAGT
ATCTGGCCATATCCGAATGAAAGAATAGCTTTTAAAGCTAAAAAATACATTGATAGAAATACTTCTGAACAATTCTCGTT
AATAGAAAAAAGAAATAATTTAGCTTTCTTAATAACTGAAAAAGAAGGAAGTATTTATGCAAAATATTTTTGGCAACATA
CTGGTCTTGGTCTATCTTCAAGAGCTGCCGCTATAGAACTAGGTCTTGAAGATTGCCCTCCAAAATCTTACGTAAATGAA
TGTTCTCAAATAATAAAAAATAGAATTTCTAAATCTACAAAAATTGACTCTAATGATATTCACTTAACTTCATCTGGAAT
GTCTGCATTGCATACATCATTAGAAATCATATATAAATTATTTCCAGCTAAACCAACACTCCAAGTTGGTTTTCCATATG
TAGATGTACTTAAATTACCAATGAAAATCTTTCACGGAGCAAAGTTAATTACAGAAGAAAATTGCGCGGATATTGAATTA
GAAATCAAAAGAATAAATCCATCAGCATTAATTATTGAACTTCCAAGTAATCCAATGCTCAAATGTGTAAACATTAAAAA
AATTTCAAAAATTGCAAAAAAGCTAAATATTCCTTTAATTGTTGACGATACAATTGGTTCGAATTTAAATATAAATTCCA
CAGAACATGCAGATATAGTTTTTACTTCACTTACAAAAATTTTTTCAGGTAGTGGTGATATTCTTGCCGGATCATTAATA
CTAAATCCAAAAAGCAAATGGATTGATCAGTTTAGAAATGCATTAAACGAGATTAATATTCCAATACTTTCCGATGGAGA
TATAGTTTATCTAGAGAAAGTTAGTAGAGATGTAAATCAAAGAGTTTTTGAACAAAATAAAGCATGTTTAGAATTAAAAA
AAAGATTAGAGAATCATAGCGAGATTAAAAATATTTTCCATCCTGAAAATTGTCCAAATTTTAATTCTTTACTTACTTCT
AATGGGGGATACGGCTGCTTATTATCGTTTGAATTAAATGGAGGATTGAACAAAGCTAAAAATTTTTATGATTCTCTAAA
AGTATCTAAAGGACCTAGTTTAGGTACAAAATTTACTCTAGTTTGTCCTTATGTTTTACTAGCTCATTATGACGAGTTGG
ATTGGGCTGAAAGTTTTGGTATACCCTCGCACCTTATTAGAGTATCAGTTGGATTAGAAGACCAAGATCAATTATGGAAA
ACCTTTTCTGAAGCACTAAATAATTTCTAA

Upstream 100 bases:

>100_bases
ACTAGGGATAGATGATGCTCTTATCAGATTATCAATTGGATGTGAAGATACAGACGATTTAATCTCGGACATTTTATTTG
CTTTAAATAAATTCTGAAAA

Downstream 100 bases:

>100_bases
ATTCCTAGTATTTACCGTATAGAAACTTATATACTCTTTTTCTGAATAATAGTTAGTATTAAAATATATTTTCTCAATAT
ATAAATGGCAAAAATTTATG

Product: putative cystathionine gamma-synthase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 489; Mature: 489

Protein sequence:

>489_residues
MRDLLKKPIWKNLELGYAIPDSIHAVSVALPTWNDVINYEEKDQECINLLKSIYPRFGLNPIVKRLCEKVKKQNYYNNKS
IWPYPNERIAFKAKKYIDRNTSEQFSLIEKRNNLAFLITEKEGSIYAKYFWQHTGLGLSSRAAAIELGLEDCPPKSYVNE
CSQIIKNRISKSTKIDSNDIHLTSSGMSALHTSLEIIYKLFPAKPTLQVGFPYVDVLKLPMKIFHGAKLITEENCADIEL
EIKRINPSALIIELPSNPMLKCVNIKKISKIAKKLNIPLIVDDTIGSNLNINSTEHADIVFTSLTKIFSGSGDILAGSLI
LNPKSKWIDQFRNALNEINIPILSDGDIVYLEKVSRDVNQRVFEQNKACLELKKRLENHSEIKNIFHPENCPNFNSLLTS
NGGYGCLLSFELNGGLNKAKNFYDSLKVSKGPSLGTKFTLVCPYVLLAHYDELDWAESFGIPSHLIRVSVGLEDQDQLWK
TFSEALNNF

Sequences:

>Translated_489_residues
MRDLLKKPIWKNLELGYAIPDSIHAVSVALPTWNDVINYEEKDQECINLLKSIYPRFGLNPIVKRLCEKVKKQNYYNNKS
IWPYPNERIAFKAKKYIDRNTSEQFSLIEKRNNLAFLITEKEGSIYAKYFWQHTGLGLSSRAAAIELGLEDCPPKSYVNE
CSQIIKNRISKSTKIDSNDIHLTSSGMSALHTSLEIIYKLFPAKPTLQVGFPYVDVLKLPMKIFHGAKLITEENCADIEL
EIKRINPSALIIELPSNPMLKCVNIKKISKIAKKLNIPLIVDDTIGSNLNINSTEHADIVFTSLTKIFSGSGDILAGSLI
LNPKSKWIDQFRNALNEINIPILSDGDIVYLEKVSRDVNQRVFEQNKACLELKKRLENHSEIKNIFHPENCPNFNSLLTS
NGGYGCLLSFELNGGLNKAKNFYDSLKVSKGPSLGTKFTLVCPYVLLAHYDELDWAESFGIPSHLIRVSVGLEDQDQLWK
TFSEALNNF
>Mature_489_residues
MRDLLKKPIWKNLELGYAIPDSIHAVSVALPTWNDVINYEEKDQECINLLKSIYPRFGLNPIVKRLCEKVKKQNYYNNKS
IWPYPNERIAFKAKKYIDRNTSEQFSLIEKRNNLAFLITEKEGSIYAKYFWQHTGLGLSSRAAAIELGLEDCPPKSYVNE
CSQIIKNRISKSTKIDSNDIHLTSSGMSALHTSLEIIYKLFPAKPTLQVGFPYVDVLKLPMKIFHGAKLITEENCADIEL
EIKRINPSALIIELPSNPMLKCVNIKKISKIAKKLNIPLIVDDTIGSNLNINSTEHADIVFTSLTKIFSGSGDILAGSLI
LNPKSKWIDQFRNALNEINIPILSDGDIVYLEKVSRDVNQRVFEQNKACLELKKRLENHSEIKNIFHPENCPNFNSLLTS
NGGYGCLLSFELNGGLNKAKNFYDSLKVSKGPSLGTKFTLVCPYVLLAHYDELDWAESFGIPSHLIRVSVGLEDQDQLWK
TFSEALNNF

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Escherichia coli, GI1790375, Length=318, Percent_Identity=25.1572327044025, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17560056, Length=265, Percent_Identity=28.6792452830189, Blast_Score=75, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17538055, Length=274, Percent_Identity=27.7372262773723, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6322970, Length=566, Percent_Identity=31.9787985865724, Blast_Score=221, Evalue=1e-58,
Organism=Saccharomyces cerevisiae, GI6322590, Length=349, Percent_Identity=33.810888252149, Blast_Score=179, Evalue=9e-46,
Organism=Saccharomyces cerevisiae, GI6323557, Length=345, Percent_Identity=34.2028985507246, Blast_Score=176, Evalue=7e-45,
Organism=Saccharomyces cerevisiae, GI6319307, Length=254, Percent_Identity=29.5275590551181, Blast_Score=77, Evalue=7e-15,
Organism=Drosophila melanogaster, GI19922554, Length=244, Percent_Identity=28.6885245901639, Blast_Score=87, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 55403; Mature: 55403

Theoretical pI: Translated: 8.37; Mature: 8.37

Prosite motif: PS00228 TUBULIN_B_AUTOREG

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRDLLKKPIWKNLELGYAIPDSIHAVSVALPTWNDVINYEEKDQECINLLKSIYPRFGLN
CCHHHHCCCCCCCCCCEECCCCCCEEEEECCCCHHHCCCCCHHHHHHHHHHHHCCCCCCC
PIVKRLCEKVKKQNYYNNKSIWPYPNERIAFKAKKYIDRNTSEQFSLIEKRNNLAFLITE
HHHHHHHHHHHHCCCCCCCCCCCCCCCCEEHHHHHHHCCCCHHHHHHHHCCCCEEEEEEE
KEGSIYAKYFWQHTGLGLSSRAAAIELGLEDCPPKSYVNECSQIIKNRISKSTKIDSNDI
CCCCEEEEEEHHHCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCE
HLTSSGMSALHTSLEIIYKLFPAKPTLQVGFPYVDVLKLPMKIFHGAKLITEENCADIEL
EEECCCHHHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHHCCCCEEECCCCCCEEE
EIKRINPSALIIELPSNPMLKCVNIKKISKIAKKLNIPLIVDDTIGSNLNINSTEHADIV
EEEEECCCEEEEECCCCCCEEEECHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCHHHH
FTSLTKIFSGSGDILAGSLILNPKSKWIDQFRNALNEINIPILSDGDIVYLEKVSRDVNQ
HHHHHHHHCCCCCEEEEEEEECCHHHHHHHHHHHHHHCCCEEECCCCEEEHHHHHHHHHH
RVFEQNKACLELKKRLENHSEIKNIFHPENCPNFNSLLTSNGGYGCLLSFELNGGLNKAK
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCEEEEEEEEECCCCHHHH
NFYDSLKVSKGPSLGTKFTLVCPYVLLAHYDELDWAESFGIPSHLIRVSVGLEDQDQLWK
HHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHCCCCHHEEEEEECCCCHHHHHH
TFSEALNNF
HHHHHHHCC
>Mature Secondary Structure
MRDLLKKPIWKNLELGYAIPDSIHAVSVALPTWNDVINYEEKDQECINLLKSIYPRFGLN
CCHHHHCCCCCCCCCCEECCCCCCEEEEECCCCHHHCCCCCHHHHHHHHHHHHCCCCCCC
PIVKRLCEKVKKQNYYNNKSIWPYPNERIAFKAKKYIDRNTSEQFSLIEKRNNLAFLITE
HHHHHHHHHHHHCCCCCCCCCCCCCCCCEEHHHHHHHCCCCHHHHHHHHCCCCEEEEEEE
KEGSIYAKYFWQHTGLGLSSRAAAIELGLEDCPPKSYVNECSQIIKNRISKSTKIDSNDI
CCCCEEEEEEHHHCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCE
HLTSSGMSALHTSLEIIYKLFPAKPTLQVGFPYVDVLKLPMKIFHGAKLITEENCADIEL
EEECCCHHHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHHCCCCEEECCCCCCEEE
EIKRINPSALIIELPSNPMLKCVNIKKISKIAKKLNIPLIVDDTIGSNLNINSTEHADIV
EEEEECCCEEEEECCCCCCEEEECHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCHHHH
FTSLTKIFSGSGDILAGSLILNPKSKWIDQFRNALNEINIPILSDGDIVYLEKVSRDVNQ
HHHHHHHHCCCCCEEEEEEEECCHHHHHHHHHHHHHHCCCEEECCCCEEEHHHHHHHHHH
RVFEQNKACLELKKRLENHSEIKNIFHPENCPNFNSLLTSNGGYGCLLSFELNGGLNKAK
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCEEEEEEEEECCCCHHHH
NFYDSLKVSKGPSLGTKFTLVCPYVLLAHYDELDWAESFGIPSHLIRVSVGLEDQDQLWK
HHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHCCCCHHEEEEEECCCCHHHHHH
TFSEALNNF
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]