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The map label for this gene is fabI [H]

Identifier: 126695644

GI number: 126695644

Start: 281380

End: 282162

Strand: Reverse

Name: fabI [H]

Synonym: P9301_03061

Alternate gene names: 126695644

Gene position: 282162-281380 (Counterclockwise)

Preceding gene: 126695647

Following gene: 126695643

Centisome position: 17.19

GC content: 38.7

Gene sequence:

>783_bases
ATGCTTCTAAATCTAACTGGCAAAAAAATTCTTGTTACGGGAATTGCCAACAATCGTTCAATAGCATGGGGTATCGCTCA
ACAACTTTCAAAAGCTGGCGCAGAACTTGGAATCACATATTTGCCTGATGATAAGGGAAGATTCGAGTCTAAAGTTAGAG
AACTAACTGAACCTTTAAACCCATCGTTATTTTTGCCTCTTGATGTTCAAAATCCAGCTCAAATTGAAGAAATCTTTAAA
AATATAAAAGACAATTGGGGGCAAATTGATGGATTAGTTCACTGCCTAGCATTTGCAGGACGCGATGAATTGATTGGAGA
TTATAGTGCTACCACTTCAGAGGGTTTTGATAGAGCTCTTAATATAAGTGCGTATTCGTTAGCACCTTTATGTAAAGCAG
CAAAACCACTTTTTAGTGATGGTGCTGGAGTTGTCTCATTAACTTATTTAGGTTCAGAAAGGGCGATTCCTAACTATAAC
GTGATGGGAGTTGCTAAAGCAGCTTTAGAAGCTTCAGTAAGATATCTTTCTGCAGAACTTGGTCCCGAAAGACAAGTCAG
AGTTAACGCAATAAGTGCTGGGCCTATAAGAACACTTGCGAGTTCTGCTATAGGTGGCATTTTAGATATGATTCACAATG
TTGAAGAAAAGGCTCCTTTACGCAGAACAGTCACTCAAACAGAAGTAGGCAATACAGCTGCTTTTTTATTAAGTGATCTC
TCTAGCGGCATTTCAGGCCAAACAATTTATGTTGATGCGGGTTACTGCATTAATGGAATGTAA

Upstream 100 bases:

>100_bases
AGTTAAAAATTCTTCGATATTTGAAAATTATTGTATATTTTGCAGTAATCCGTAAAGATAGGTACTTTTATATAGGAGAA
TAATAGAAATAATAAACAAA

Downstream 100 bases:

>100_bases
ACTAAGTTTTTTGCATAAAAATGTCATCTCTAAGGCAATCTGAAATCAAAAGAAAAACGAATGAAACAGATATTTCTGTA
TTTATAAACTTAGATGGAAA

Product: enoyl-(acyl carrier protein) reductase

Products: NA

Alternate protein names: NADH-dependent enoyl-ACP reductase [H]

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MLLNLTGKKILVTGIANNRSIAWGIAQQLSKAGAELGITYLPDDKGRFESKVRELTEPLNPSLFLPLDVQNPAQIEEIFK
NIKDNWGQIDGLVHCLAFAGRDELIGDYSATTSEGFDRALNISAYSLAPLCKAAKPLFSDGAGVVSLTYLGSERAIPNYN
VMGVAKAALEASVRYLSAELGPERQVRVNAISAGPIRTLASSAIGGILDMIHNVEEKAPLRRTVTQTEVGNTAAFLLSDL
SSGISGQTIYVDAGYCINGM

Sequences:

>Translated_260_residues
MLLNLTGKKILVTGIANNRSIAWGIAQQLSKAGAELGITYLPDDKGRFESKVRELTEPLNPSLFLPLDVQNPAQIEEIFK
NIKDNWGQIDGLVHCLAFAGRDELIGDYSATTSEGFDRALNISAYSLAPLCKAAKPLFSDGAGVVSLTYLGSERAIPNYN
VMGVAKAALEASVRYLSAELGPERQVRVNAISAGPIRTLASSAIGGILDMIHNVEEKAPLRRTVTQTEVGNTAAFLLSDL
SSGISGQTIYVDAGYCINGM
>Mature_260_residues
MLLNLTGKKILVTGIANNRSIAWGIAQQLSKAGAELGITYLPDDKGRFESKVRELTEPLNPSLFLPLDVQNPAQIEEIFK
NIKDNWGQIDGLVHCLAFAGRDELIGDYSATTSEGFDRALNISAYSLAPLCKAAKPLFSDGAGVVSLTYLGSERAIPNYN
VMGVAKAALEASVRYLSAELGPERQVRVNAISAGPIRTLASSAIGGILDMIHNVEEKAPLRRTVTQTEVGNTAAFLLSDL
SSGISGQTIYVDAGYCINGM

Specific function: Fatty acid biosynthesis pathway; second reduction step. [C]

COG id: COG0623

COG function: function code I; Enoyl-[acyl-carrier-protein] reductase (NADH)

Gene ontology:

Cell location: Inner Membrane-Associated [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily [H]

Homologues:

Organism=Homo sapiens, GI7705925, Length=260, Percent_Identity=26.9230769230769, Blast_Score=72, Evalue=7e-13,
Organism=Escherichia coli, GI1787545, Length=257, Percent_Identity=50.1945525291829, Blast_Score=251, Evalue=5e-68,
Organism=Escherichia coli, GI1789378, Length=244, Percent_Identity=24.5901639344262, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI87082100, Length=260, Percent_Identity=28.0769230769231, Blast_Score=71, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI115534694, Length=258, Percent_Identity=27.906976744186, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24643142, Length=264, Percent_Identity=26.5151515151515, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI28571526, Length=258, Percent_Identity=30.6201550387597, Blast_Score=66, Evalue=3e-11,
Organism=Drosophila melanogaster, GI21357041, Length=258, Percent_Identity=29.4573643410853, Blast_Score=65, Evalue=5e-11,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR014358
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.3.1.9 [H]

Molecular weight: Translated: 27759; Mature: 27759

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLNLTGKKILVTGIANNRSIAWGIAQQLSKAGAELGITYLPDDKGRFESKVRELTEPLN
CEEECCCCEEEEEECCCCCEEHHHHHHHHHHCCHHCCEEECCCCCHHHHHHHHHHHCCCC
PSLFLPLDVQNPAQIEEIFKNIKDNWGQIDGLVHCLAFAGRDELIGDYSATTSEGFDRAL
CCEEEEECCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCHHHCCCCCCCCHHHHHHEE
NISAYSLAPLCKAAKPLFSDGAGVVSLTYLGSERAIPNYNVMGVAKAALEASVRYLSAEL
CCCHHHHHHHHHHHCHHHCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHC
GPERQVRVNAISAGPIRTLASSAIGGILDMIHNVEEKAPLRRTVTQTEVGNTAAFLLSDL
CCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCHHHHHHHHH
SSGISGQTIYVDAGYCINGM
HCCCCCCEEEEECCEEECCC
>Mature Secondary Structure
MLLNLTGKKILVTGIANNRSIAWGIAQQLSKAGAELGITYLPDDKGRFESKVRELTEPLN
CEEECCCCEEEEEECCCCCEEHHHHHHHHHHCCHHCCEEECCCCCHHHHHHHHHHHCCCC
PSLFLPLDVQNPAQIEEIFKNIKDNWGQIDGLVHCLAFAGRDELIGDYSATTSEGFDRAL
CCEEEEECCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCHHHCCCCCCCCHHHHHHEE
NISAYSLAPLCKAAKPLFSDGAGVVSLTYLGSERAIPNYNVMGVAKAALEASVRYLSAEL
CCCHHHHHHHHHHHCHHHCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHC
GPERQVRVNAISAGPIRTLASSAIGGILDMIHNVEEKAPLRRTVTQTEVGNTAAFLLSDL
CCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCHHHHHHHHH
SSGISGQTIYVDAGYCINGM
HCCCCCCEEEEECCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8391534; 11759840 [H]