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The map label for this gene is phrB [H]

Identifier: 126695647

GI number: 126695647

Start: 284105

End: 285541

Strand: Reverse

Name: phrB [H]

Synonym: P9301_03091

Alternate gene names: 126695647

Gene position: 285541-284105 (Counterclockwise)

Preceding gene: 126695648

Following gene: 126695644

Centisome position: 17.39

GC content: 30.55

Gene sequence:

>1437_bases
ATGAATAAACCTAGAATACTTTTTTGGCATAGAAAGGATTTAAGAATATTTGACAATCAATCTTTAATTAAAGCATTTTC
ATTATCAAATGCTATTACTTCGACTTACATATTTGATAAAAATTACCCGCACGATTTCAATGCAAATTCAAGAGCTTGGT
TTCTAGGAAATTCGCTTCAAGAATTAGGAAATAATTGGAAAAAAATGGGTAGTAGATTAATCATGGAAGAAGGAGATCCG
GTATTAATAATTCCTCAATTAGCAAAGAAAATAGATGCTAAATTTGTTTTTTGGAATAAATCAATTGAACCTTATGAGAT
TAATCGCGATTTAAAAATAAAAAAAAATTTAGAAGAACAAAATATTCAAGTTATTGAAACTTGGGATCACTTATTAATAG
AACCTTTAAAAATATTTTCCGGAAATAATAAACCTTATTCAGTTTATGGGCCTTTTTATAAAAACCTTAAATCAAAAATG
AATTTATTAGGTCCATATGACCAAGATAAAGTTGTTTTCCAGTTTAAAGATATAGATAATAAACTCAAAGAAAATAAGAC
AATAAATTCATCTGATTCTGTTCTAGAGAAATTTATCAAAAATATAAAATTTCCTGGTTCGAATATTTGTCCATGTAAAC
CTGGAGAGAATGCTGCAGAGACAATATTAGAAAACTTCATTAACGAAAAAAAAATATATTCTTATGATTCTGCACGAGAT
TTTCCTTCCCATAATGGGACATCTTTTCTAAGTGCATCTCTCAGATTCGGCACCATTAGCATTAGAAAAATTTGGAACGC
CACATTAAATTTAAATTCAGATTGTGCAAATCGAGTAAATTATCTATCAATTGAAACTTGGCAAAAAGAACTTGTTTGGC
GTGAATTTTATCAACATTGCTTATTCCATTTCCCAGAGCTAGAGAAAGGTCCATATAGAAAAAAATGGGATCACTTTCCA
TGGCAAAACAATAATGAATGGTTTCAGCATTGGAGCAACGGAGAGACCGGAGTACCTATAGTTGATGCTGCAATGCGTCA
ACTAAATAGTACTGGCTGGATGCATAACAGATGTAGGATGATAGTCGCTTCATTTCTGGTAAAAGATCTTATATGCAATT
GGCAAATGGGAGAGAAAAAATTTATGGAGACATTGGTTGATGGAGACTTAGCTGCAAATAATGGGGGATGGCAGTGGAGC
GCCAGTAGCGGTATGGATCCAAAACCACTTAGAATTTTTAATCCATATACCCAAGCAAAAAAATTTGATCCTATTTGCGA
ATATATAAAATATTGGATTCCTGAATTATCTAAAGTGTCAAATTCAGAATTATTAAATGGAGAGATATCTAATTTAGAAA
AAAATAATTATTCAAGCCCTATTGTCAATCACAACATACAACAAAGATTATTTAAATCACTTTATGCTGAAATTTGA

Upstream 100 bases:

>100_bases
ATCCGAACGAACTAGATAATCTTATTTCTAGTGGAGATGAGATTCTTGACGCAAAAACTGTGACAGCTTGGTTTAGAGCT
AAACAATTTTTAGATAAATT

Downstream 100 bases:

>100_bases
ATTTCCTGTATACAATTCTTTAAAACTTTATTTAAATTTTCTGCTACATAAACTTGCTCTTCATAAGAAATTTCAGGAAA
CATCGGAAGACTAAGAACTT

Product: putative DNA photolyase

Products: NA

Alternate protein names: DNA photolyase; Photoreactivating enzyme [H]

Number of amino acids: Translated: 478; Mature: 478

Protein sequence:

>478_residues
MNKPRILFWHRKDLRIFDNQSLIKAFSLSNAITSTYIFDKNYPHDFNANSRAWFLGNSLQELGNNWKKMGSRLIMEEGDP
VLIIPQLAKKIDAKFVFWNKSIEPYEINRDLKIKKNLEEQNIQVIETWDHLLIEPLKIFSGNNKPYSVYGPFYKNLKSKM
NLLGPYDQDKVVFQFKDIDNKLKENKTINSSDSVLEKFIKNIKFPGSNICPCKPGENAAETILENFINEKKIYSYDSARD
FPSHNGTSFLSASLRFGTISIRKIWNATLNLNSDCANRVNYLSIETWQKELVWREFYQHCLFHFPELEKGPYRKKWDHFP
WQNNNEWFQHWSNGETGVPIVDAAMRQLNSTGWMHNRCRMIVASFLVKDLICNWQMGEKKFMETLVDGDLAANNGGWQWS
ASSGMDPKPLRIFNPYTQAKKFDPICEYIKYWIPELSKVSNSELLNGEISNLEKNNYSSPIVNHNIQQRLFKSLYAEI

Sequences:

>Translated_478_residues
MNKPRILFWHRKDLRIFDNQSLIKAFSLSNAITSTYIFDKNYPHDFNANSRAWFLGNSLQELGNNWKKMGSRLIMEEGDP
VLIIPQLAKKIDAKFVFWNKSIEPYEINRDLKIKKNLEEQNIQVIETWDHLLIEPLKIFSGNNKPYSVYGPFYKNLKSKM
NLLGPYDQDKVVFQFKDIDNKLKENKTINSSDSVLEKFIKNIKFPGSNICPCKPGENAAETILENFINEKKIYSYDSARD
FPSHNGTSFLSASLRFGTISIRKIWNATLNLNSDCANRVNYLSIETWQKELVWREFYQHCLFHFPELEKGPYRKKWDHFP
WQNNNEWFQHWSNGETGVPIVDAAMRQLNSTGWMHNRCRMIVASFLVKDLICNWQMGEKKFMETLVDGDLAANNGGWQWS
ASSGMDPKPLRIFNPYTQAKKFDPICEYIKYWIPELSKVSNSELLNGEISNLEKNNYSSPIVNHNIQQRLFKSLYAEI
>Mature_478_residues
MNKPRILFWHRKDLRIFDNQSLIKAFSLSNAITSTYIFDKNYPHDFNANSRAWFLGNSLQELGNNWKKMGSRLIMEEGDP
VLIIPQLAKKIDAKFVFWNKSIEPYEINRDLKIKKNLEEQNIQVIETWDHLLIEPLKIFSGNNKPYSVYGPFYKNLKSKM
NLLGPYDQDKVVFQFKDIDNKLKENKTINSSDSVLEKFIKNIKFPGSNICPCKPGENAAETILENFINEKKIYSYDSARD
FPSHNGTSFLSASLRFGTISIRKIWNATLNLNSDCANRVNYLSIETWQKELVWREFYQHCLFHFPELEKGPYRKKWDHFP
WQNNNEWFQHWSNGETGVPIVDAAMRQLNSTGWMHNRCRMIVASFLVKDLICNWQMGEKKFMETLVDGDLAANNGGWQWS
ASSGMDPKPLRIFNPYTQAKKFDPICEYIKYWIPELSKVSNSELLNGEISNLEKNNYSSPIVNHNIQQRLFKSLYAEI

Specific function: Involved in repair of UV radiation-induced DNA damage. Catalyzes the light-dependent monomerization (300-600 nm) of cyclobutyl pyrimidine dimers (in cis-syn configuration), which are formed between adjacent bases on the same DNA strand upon exposure to ul

COG id: COG0415

COG function: function code L; Deoxyribodipyrimidine photolyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DNA photolyase domain [H]

Homologues:

Organism=Homo sapiens, GI188536100, Length=502, Percent_Identity=26.6932270916335, Blast_Score=170, Evalue=3e-42,
Organism=Homo sapiens, GI4758072, Length=500, Percent_Identity=28.2, Blast_Score=165, Evalue=8e-41,
Organism=Homo sapiens, GI188536103, Length=460, Percent_Identity=26.5217391304348, Blast_Score=152, Evalue=7e-37,
Organism=Escherichia coli, GI1786926, Length=447, Percent_Identity=37.3601789709172, Blast_Score=251, Evalue=1e-67,
Organism=Saccharomyces cerevisiae, GI6324962, Length=494, Percent_Identity=30.3643724696356, Blast_Score=197, Evalue=3e-51,
Organism=Drosophila melanogaster, GI17137248, Length=454, Percent_Identity=27.7533039647577, Blast_Score=171, Evalue=8e-43,
Organism=Drosophila melanogaster, GI24585455, Length=454, Percent_Identity=27.7533039647577, Blast_Score=171, Evalue=8e-43,
Organism=Drosophila melanogaster, GI24648152, Length=466, Percent_Identity=26.1802575107296, Blast_Score=143, Evalue=3e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002081
- InterPro:   IPR018394
- InterPro:   IPR006050
- InterPro:   IPR019947
- InterPro:   IPR005101
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00875 DNA_photolyase; PF03441 FAD_binding_7 [H]

EC number: =4.1.99.3 [H]

Molecular weight: Translated: 55983; Mature: 55983

Theoretical pI: Translated: 9.01; Mature: 9.01

Prosite motif: PS00394 DNA_PHOTOLYASES_1_1 ; PS00691 DNA_PHOTOLYASES_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKPRILFWHRKDLRIFDNQSLIKAFSLSNAITSTYIFDKNYPHDFNANSRAWFLGNSLQ
CCCCCEEEEECCCCEEECCHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCEEEECCCHHH
ELGNNWKKMGSRLIMEEGDPVLIIPQLAKKIDAKFVFWNKSIEPYEINRDLKIKKNLEEQ
HHHHHHHHHCCEEEEECCCCEEECHHHHHHHCCEEEEECCCCCCEECCCCCCHHCCCCCC
NIQVIETWDHLLIEPLKIFSGNNKPYSVYGPFYKNLKSKMNLLGPYDQDKVVFQFKDIDN
CCEEEEHHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHCCCCCCCCEEEEEECHHH
KLKENKTINSSDSVLEKFIKNIKFPGSNICPCKPGENAAETILENFINEKKIYSYDSARD
HHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEECCCCCCC
FPSHNGTSFLSASLRFGTISIRKIWNATLNLNSDCANRVNYLSIETWQKELVWREFYQHC
CCCCCCCHHEEEEEEECEEEHHHHHHHHCCCCCHHHHCCCEEEHHHHHHHHHHHHHHHHH
LFHFPELEKGPYRKKWDHFPWQNNNEWFQHWSNGETGVPIVDAAMRQLNSTGWMHNRCRM
HHCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHHCCCCHHHHHHH
IVASFLVKDLICNWQMGEKKFMETLVDGDLAANNGGWQWSASSGMDPKPLRIFNPYTQAK
HHHHHHHHHHHHCCCCCHHHHHHHHHCCCEEECCCCEEECCCCCCCCCCEEEECCHHHHH
KFDPICEYIKYWIPELSKVSNSELLNGEISNLEKNNYSSPIVNHNIQQRLFKSLYAEI
CCCHHHHHHHHHHHHHHHCCCCHHCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MNKPRILFWHRKDLRIFDNQSLIKAFSLSNAITSTYIFDKNYPHDFNANSRAWFLGNSLQ
CCCCCEEEEECCCCEEECCHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCEEEECCCHHH
ELGNNWKKMGSRLIMEEGDPVLIIPQLAKKIDAKFVFWNKSIEPYEINRDLKIKKNLEEQ
HHHHHHHHHCCEEEEECCCCEEECHHHHHHHCCEEEEECCCCCCEECCCCCCHHCCCCCC
NIQVIETWDHLLIEPLKIFSGNNKPYSVYGPFYKNLKSKMNLLGPYDQDKVVFQFKDIDN
CCEEEEHHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHCCCCCCCCEEEEEECHHH
KLKENKTINSSDSVLEKFIKNIKFPGSNICPCKPGENAAETILENFINEKKIYSYDSARD
HHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEECCCCCCC
FPSHNGTSFLSASLRFGTISIRKIWNATLNLNSDCANRVNYLSIETWQKELVWREFYQHC
CCCCCCCHHEEEEEEECEEEHHHHHHHHCCCCCHHHHCCCEEEHHHHHHHHHHHHHHHHH
LFHFPELEKGPYRKKWDHFPWQNNNEWFQHWSNGETGVPIVDAAMRQLNSTGWMHNRCRM
HHCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHHCCCCHHHHHHH
IVASFLVKDLICNWQMGEKKFMETLVDGDLAANNGGWQWSASSGMDPKPLRIFNPYTQAK
HHHHHHHHHHHHCCCCCHHHHHHHHHCCCEEECCCCEEECCCCCCCCCCEEEECCHHHHH
KFDPICEYIKYWIPELSKVSNSELLNGEISNLEKNNYSSPIVNHNIQQRLFKSLYAEI
CCCHHHHHHHHHHHHHHHCCCCHHCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2837735; 2110564; 9360600 [H]