Click here to switch to the map view.

The map label for this gene is nitA [H]

Identifier: 126700458

GI number: 126700458

Start: 3324267

End: 3325193

Strand: Direct

Name: nitA [H]

Synonym: CD2842

Alternate gene names: 126700458

Gene position: 3324267-3325193 (Clockwise)

Preceding gene: 126700445

Following gene: 126700460

Centisome position: 77.48

GC content: 34.84

Gene sequence:

>927_bases
ATGAGCAAGGAAAATAAAAATGTAAGAATTGCTGTTGTCCAGGCGTCACCAGTCATAATGGATTTAGAAAAAACTGTTGA
AAAAGCATTAGGATTAATTAAAGAAGCTGGTAGAAAAGGGGCAAATATCGTAGTTTTCCCTGAAGCATTCATACCTGCAT
ATCCAAGAGGTCTTTCATTTGGATTTGTTGTTGGAAGTAGAACAATGGAAGGTAGAGAAGATTGGAAAAGATACTATGAT
AATTCTGTACCAGTTCCTAGTGCTACAACAGATTTATTAGGAAAAGCAGCACAAGAAGCAGGAGTATATCTATCAATGGG
TATAACAGAAAGAGATGGTAATGATATAAATTGTACCCTTTATTGCACAAACTTATTTTTCTCTCCAGAAGGTAAGCTAA
TTGGTAAACACAGAAAACTTAAACCTACAGGAACAGAAAGATGTATTTGGGGAGAAGGAGATGGAAGTACTCTTACAGTA
GTTGATACTCCATATGGAAAAATGGGTTCGTTGATTTGTTGGGAAAATTATATGCCATTAGCTAGAACAGCTCTTTATGC
TAAGGGTGTAAAACTTTACATAGCCCCTACTGCAGATTCAAGAGAAGAGTGGCAAGCAACTATGAAGCATATTGCTTTAG
AAGGAAGATGTTTTGTAATTGGATGCAATCAATATGTTGAAAAAAATATGTACCCAACTGACCTTAACTACTATAAGGAG
CTAGATGCAGAACCAGAAATTATGTGTCCAGGAGGAAGTTGTATAGTTGACCCTTTTGGAAAATATGTAGCAGGTCCTAT
TTTCAATAAAGAAGAAATGCTAATAGCTGATTTAGACCTAGAAAAAATAGTACTTAGTAGACTAGATTTTGATTCAGAAG
GTCACTATTCAAGACCAGATGTATTTGAATTAATTGTTCATGAATAA

Upstream 100 bases:

>100_bases
GTTACATTATTATTCACAATATAAGTATCTTAGTTCTCAAATAACATCTCATGTAATCTATTCAATGCAAAAAATTTAAA
TATTTTTTAGGAGGTTTTTT

Downstream 100 bases:

>100_bases
TCTTTTTACACAAACATCTACATAAATCTTTTTACATAAAAAAATGAGATACTCATAAGTAAATTTCAAGTATCTCATTT
TTGCTTAAAACCTATAATCC

Product: nitrilase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 308; Mature: 307

Protein sequence:

>308_residues
MSKENKNVRIAVVQASPVIMDLEKTVEKALGLIKEAGRKGANIVVFPEAFIPAYPRGLSFGFVVGSRTMEGREDWKRYYD
NSVPVPSATTDLLGKAAQEAGVYLSMGITERDGNDINCTLYCTNLFFSPEGKLIGKHRKLKPTGTERCIWGEGDGSTLTV
VDTPYGKMGSLICWENYMPLARTALYAKGVKLYIAPTADSREEWQATMKHIALEGRCFVIGCNQYVEKNMYPTDLNYYKE
LDAEPEIMCPGGSCIVDPFGKYVAGPIFNKEEMLIADLDLEKIVLSRLDFDSEGHYSRPDVFELIVHE

Sequences:

>Translated_308_residues
MSKENKNVRIAVVQASPVIMDLEKTVEKALGLIKEAGRKGANIVVFPEAFIPAYPRGLSFGFVVGSRTMEGREDWKRYYD
NSVPVPSATTDLLGKAAQEAGVYLSMGITERDGNDINCTLYCTNLFFSPEGKLIGKHRKLKPTGTERCIWGEGDGSTLTV
VDTPYGKMGSLICWENYMPLARTALYAKGVKLYIAPTADSREEWQATMKHIALEGRCFVIGCNQYVEKNMYPTDLNYYKE
LDAEPEIMCPGGSCIVDPFGKYVAGPIFNKEEMLIADLDLEKIVLSRLDFDSEGHYSRPDVFELIVHE
>Mature_307_residues
SKENKNVRIAVVQASPVIMDLEKTVEKALGLIKEAGRKGANIVVFPEAFIPAYPRGLSFGFVVGSRTMEGREDWKRYYDN
SVPVPSATTDLLGKAAQEAGVYLSMGITERDGNDINCTLYCTNLFFSPEGKLIGKHRKLKPTGTERCIWGEGDGSTLTVV
DTPYGKMGSLICWENYMPLARTALYAKGVKLYIAPTADSREEWQATMKHIALEGRCFVIGCNQYVEKNMYPTDLNYYKEL
DAEPEIMCPGGSCIVDPFGKYVAGPIFNKEEMLIADLDLEKIVLSRLDFDSEGHYSRPDVFELIVHE

Specific function: Unknown

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI9910460, Length=297, Percent_Identity=25.5892255892256, Blast_Score=67, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17557111, Length=300, Percent_Identity=46.6666666666667, Blast_Score=291, Evalue=3e-79,
Organism=Saccharomyces cerevisiae, GI6322027, Length=196, Percent_Identity=42.8571428571429, Blast_Score=160, Evalue=2e-40,
Organism=Saccharomyces cerevisiae, GI6322026, Length=113, Percent_Identity=34.5132743362832, Blast_Score=72, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6323383, Length=296, Percent_Identity=26.3513513513513, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000132
- InterPro:   IPR003010 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: =3.5.5.7 [H]

Molecular weight: Translated: 34357; Mature: 34226

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKENKNVRIAVVQASPVIMDLEKTVEKALGLIKEAGRKGANIVVFPEAFIPAYPRGLSF
CCCCCCCEEEEEEECCCEEEHHHHHHHHHHHHHHHHCCCCCEEEEECHHHCCCCCCCCEE
GFVVGSRTMEGREDWKRYYDNSVPVPSATTDLLGKAAQEAGVYLSMGITERDGNDINCTL
EEEEECCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCEEEEEEEEECCCCEEEEEE
YCTNLFFSPEGKLIGKHRKLKPTGTERCIWGEGDGSTLTVVDTPYGKMGSLICWENYMPL
EEEEEEECCCCCEECCCCCCCCCCCCEEEEECCCCCEEEEEECCCCCCCCEEEECCCCCH
ARTALYAKGVKLYIAPTADSREEWQATMKHIALEGRCFVIGCNQYVEKNMYPTDLNYYKE
HHHHHHHCCEEEEEEECCCCHHHHHHHHHHHEECCEEEEEECHHHHHCCCCCCCCHHHHH
LDAEPEIMCPGGSCIVDPFGKYVAGPIFNKEEMLIADLDLEKIVLSRLDFDSEGHYSRPD
CCCCCCEECCCCCEEECCCHHHHCCCCCCCCCEEEEECCHHHHHHHHCCCCCCCCCCCCC
VFELIVHE
CEEEEECC
>Mature Secondary Structure 
SKENKNVRIAVVQASPVIMDLEKTVEKALGLIKEAGRKGANIVVFPEAFIPAYPRGLSF
CCCCCCEEEEEEECCCEEEHHHHHHHHHHHHHHHHCCCCCEEEEECHHHCCCCCCCCEE
GFVVGSRTMEGREDWKRYYDNSVPVPSATTDLLGKAAQEAGVYLSMGITERDGNDINCTL
EEEEECCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCEEEEEEEEECCCCEEEEEE
YCTNLFFSPEGKLIGKHRKLKPTGTERCIWGEGDGSTLTVVDTPYGKMGSLICWENYMPL
EEEEEEECCCCCEECCCCCCCCCCCCEEEEECCCCCEEEEEECCCCCCCCEEEECCCCCH
ARTALYAKGVKLYIAPTADSREEWQATMKHIALEGRCFVIGCNQYVEKNMYPTDLNYYKE
HHHHHHHCCEEEEEEECCCCHHHHHHHHHHHEECCEEEEEECHHHHHCCCCCCCCHHHHH
LDAEPEIMCPGGSCIVDPFGKYVAGPIFNKEEMLIADLDLEKIVLSRLDFDSEGHYSRPD
CCCCCCEECCCCCEEECCCHHHHCCCCCCCCCEEEEECCHHHHHHHHCCCCCCCCCCCCC
VFELIVHE
CEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1400390 [H]