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The map label for this gene is appA [H]

Identifier: 126700286

GI number: 126700286

Start: 3087152

End: 3088711

Strand: Direct

Name: appA [H]

Synonym: CD2672

Alternate gene names: 126700286

Gene position: 3087152-3088711 (Clockwise)

Preceding gene: 126700279

Following gene: 126700287

Centisome position: 71.96

GC content: 30.45

Gene sequence:

>1560_bases
ATGAAATTTAAAAAATTGGCATCATTAATCTTAGTTTCATCATTAATGCTTACATTTACAGCCTGTGCTTCCAATGATAA
AGACAAGTCTAATGGAAGCAAATCTGGTGGAACTGTTGTAATACCTATGGCAAGTGACCCAGATATAATCAATGGAGCTT
TTGCAAACGTTAAAGAAGACTCTCTTGCATCAAATATAGTTTATGCTCCTCTTTATACTTATGAAAAAGGAAATTTAGTA
AATTATTTAGCAGAAAAAGTAGATTTTAAAGATAGTAAGGAATTAACTATAAAATTAAAATCCAATTTAAAATGGCATGA
TGGAAAGCCAATAACTGCTGAAGATGTCTTATTTACATTTAATACAGTTTTAGACGAAAAACAAAATTCACCATCAAGAC
AATACTTATTGGTGGGTGAGAAACCAGTTAAAGTAGAAAAAATTGATGATTTAACAGTTAAGATTACTTTACCAACTGCA
AGTGAATCTTTCTTATATGGTATATCTAAAATTTCTCCAATACCAAAACATGTGTTTGAAGGAGAATCAAATATAGCTAA
GTCAGAAAAAAATAATAATCCAGTTGGTTCTGGTGCATTCAAGTTTAAGGAATGGAAAAAAGGTGAAAGCATAGTATTTG
AAAAGAATGCAGATTATTTTGGTGGAGAACCTAAGGCAGATTCTATAGCGCTAAAAATAATTCCTAATGAAGCATCTCAA
GAAGCAGCTTTAAATAATGGCGAAATTTCACTTATGAAGACATCTGCTGAAGGATATGAAAAGGCAAAATCAAACTCAAA
CTTACAAACTTATACTTATAGTGAAGAAAGATTAAACTACATTGTATTTAACCAAAATATATCAAACATGGCTAACAAAG
AAGTAAGACAAGCCTTATCATATGCCTTAAATAGAAATGAAATGATAGAATCAGCATATGGAAAAGAAGGTTCTGTACCT
GCTAAATCTATATTAGTTCCAGAAGCAGACTTCTATACAGAAGAAGGTGTTGAAGGATATGACCAAGATACAAATAAGGC
TAAAGACTTATTGGATAAAAGTGGTGTTAAAATTGATAAATTAAAAATAGGTTATAACACAGGTAGATTTGGGCATAAAA
ACTATGCCTTAGTAGCTCAACAAGAGTTGAAAAAAATTGGTATAGAAGCTGAAATAGTTCCTTATGAAAGTAAGGCATTC
TTCAATATACTATTTAGTAATAGCACTGAATGTGATATGTATGTAAATGGTTATGCTTGGGGATTAGAACCAAACCCATA
TAGAGGTATGTTTGAAACTGGTCAATATTGCAACCAAACTAAATATTCTAATGCTGAAATAGATGCTTTATGGGAAAAAG
GATTTACTGAGTTAAACAAGGAAAAACGTGAAGAAATATATAAGCAAATACAACAGGATATATCTAAGGATGCGCCTATT
TACACTATAGATTATGAACAAAATTTAATGGCAGCTCAAAAAAATCTAAAAGGAATAAAAGATGCTAAGCCATCTCCTGC
AATATTATTTGAAGATTGGTCTAAACTGTATGTAGAATAA

Upstream 100 bases:

>100_bases
ATATAATAGTTAATTTCTATATATATTGATTTAATAATGATTACTATTATACAATAAAAAATATGTGTTAAATATTATTT
TATGGAGGTTTTATAAGAAT

Downstream 100 bases:

>100_bases
TTGATGTAAAATTTTATATTATTAATTAGAAAAGGAGATGTCTAAAAATAATATTGTTTTATTATTTTAAAACGAGACAT
CTCTTTTAATTAGGAGAATT

Product: oligopeptide ABC transporter substrate-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 519; Mature: 519

Protein sequence:

>519_residues
MKFKKLASLILVSSLMLTFTACASNDKDKSNGSKSGGTVVIPMASDPDIINGAFANVKEDSLASNIVYAPLYTYEKGNLV
NYLAEKVDFKDSKELTIKLKSNLKWHDGKPITAEDVLFTFNTVLDEKQNSPSRQYLLVGEKPVKVEKIDDLTVKITLPTA
SESFLYGISKISPIPKHVFEGESNIAKSEKNNNPVGSGAFKFKEWKKGESIVFEKNADYFGGEPKADSIALKIIPNEASQ
EAALNNGEISLMKTSAEGYEKAKSNSNLQTYTYSEERLNYIVFNQNISNMANKEVRQALSYALNRNEMIESAYGKEGSVP
AKSILVPEADFYTEEGVEGYDQDTNKAKDLLDKSGVKIDKLKIGYNTGRFGHKNYALVAQQELKKIGIEAEIVPYESKAF
FNILFSNSTECDMYVNGYAWGLEPNPYRGMFETGQYCNQTKYSNAEIDALWEKGFTELNKEKREEIYKQIQQDISKDAPI
YTIDYEQNLMAAQKNLKGIKDAKPSPAILFEDWSKLYVE

Sequences:

>Translated_519_residues
MKFKKLASLILVSSLMLTFTACASNDKDKSNGSKSGGTVVIPMASDPDIINGAFANVKEDSLASNIVYAPLYTYEKGNLV
NYLAEKVDFKDSKELTIKLKSNLKWHDGKPITAEDVLFTFNTVLDEKQNSPSRQYLLVGEKPVKVEKIDDLTVKITLPTA
SESFLYGISKISPIPKHVFEGESNIAKSEKNNNPVGSGAFKFKEWKKGESIVFEKNADYFGGEPKADSIALKIIPNEASQ
EAALNNGEISLMKTSAEGYEKAKSNSNLQTYTYSEERLNYIVFNQNISNMANKEVRQALSYALNRNEMIESAYGKEGSVP
AKSILVPEADFYTEEGVEGYDQDTNKAKDLLDKSGVKIDKLKIGYNTGRFGHKNYALVAQQELKKIGIEAEIVPYESKAF
FNILFSNSTECDMYVNGYAWGLEPNPYRGMFETGQYCNQTKYSNAEIDALWEKGFTELNKEKREEIYKQIQQDISKDAPI
YTIDYEQNLMAAQKNLKGIKDAKPSPAILFEDWSKLYVE
>Mature_519_residues
MKFKKLASLILVSSLMLTFTACASNDKDKSNGSKSGGTVVIPMASDPDIINGAFANVKEDSLASNIVYAPLYTYEKGNLV
NYLAEKVDFKDSKELTIKLKSNLKWHDGKPITAEDVLFTFNTVLDEKQNSPSRQYLLVGEKPVKVEKIDDLTVKITLPTA
SESFLYGISKISPIPKHVFEGESNIAKSEKNNNPVGSGAFKFKEWKKGESIVFEKNADYFGGEPKADSIALKIIPNEASQ
EAALNNGEISLMKTSAEGYEKAKSNSNLQTYTYSEERLNYIVFNQNISNMANKEVRQALSYALNRNEMIESAYGKEGSVP
AKSILVPEADFYTEEGVEGYDQDTNKAKDLLDKSGVKIDKLKIGYNTGRFGHKNYALVAQQELKKIGIEAEIVPYESKAF
FNILFSNSTECDMYVNGYAWGLEPNPYRGMFETGQYCNQTKYSNAEIDALWEKGFTELNKEKREEIYKQIQQDISKDAPI
YTIDYEQNLMAAQKNLKGIKDAKPSPAILFEDWSKLYVE

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence. AppA can bind and transport tetra- and pentapep

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787762, Length=432, Percent_Identity=28.2407407407407, Blast_Score=125, Evalue=5e-30,
Organism=Escherichia coli, GI1789966, Length=452, Percent_Identity=25.4424778761062, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1787495, Length=489, Percent_Identity=25.5623721881391, Blast_Score=115, Evalue=6e-27,
Organism=Escherichia coli, GI1789887, Length=489, Percent_Identity=22.6993865030675, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI1787052, Length=542, Percent_Identity=23.8007380073801, Blast_Score=102, Evalue=8e-23,
Organism=Escherichia coli, GI87081878, Length=457, Percent_Identity=24.945295404814, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1789397, Length=449, Percent_Identity=22.9398663697105, Blast_Score=86, Evalue=6e-18,
Organism=Escherichia coli, GI1787551, Length=414, Percent_Identity=20.2898550724638, Blast_Score=79, Evalue=1e-15,
Organism=Escherichia coli, GI87082063, Length=283, Percent_Identity=23.6749116607774, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 58164; Mature: 58164

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFKKLASLILVSSLMLTFTACASNDKDKSNGSKSGGTVVIPMASDPDIINGAFANVKED
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCCCCHHCCCCCH
SLASNIVYAPLYTYEKGNLVNYLAEKVDFKDSKELTIKLKSNLKWHDGKPITAEDVLFTF
HHHCCEEEEEEEEECCCCHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHH
NTVLDEKQNSPSRQYLLVGEKPVKVEKIDDLTVKITLPTASESFLYGISKISPIPKHVFE
HHHHHHCCCCCCCEEEEECCCCCEEEEECCEEEEEEECCCCCHHHHHHHHCCCCHHHHHC
GESNIAKSEKNNNPVGSGAFKFKEWKKGESIVFEKNADYFGGEPKADSIALKIIPNEASQ
CCCHHHHCCCCCCCCCCCCEECHHHCCCCEEEEECCCCCCCCCCCCCCEEEEEECCCCCH
EAALNNGEISLMKTSAEGYEKAKSNSNLQTYTYSEERLNYIVFNQNISNMANKEVRQALS
HHCCCCCCEEEEEECCHHHHHHCCCCCCEEEEEHHHCCEEEEEECCHHHHHHHHHHHHHH
YALNRNEMIESAYGKEGSVPAKSILVPEADFYTEEGVEGYDQDTNKAKDLLDKSGVKIDK
HHHCHHHHHHHHCCCCCCCCHHHEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEE
LKIGYNTGRFGHKNYALVAQQELKKIGIEAEIVPYESKAFFNILFSNSTECDMYVNGYAW
EEEECCCCCCCCCCEEEEEHHHHHHCCCCEEEEECCCCEEEEEEECCCCCCEEEECCEEE
GLEPNPYRGMFETGQYCNQTKYSNAEIDALWEKGFTELNKEKREEIYKQIQQDISKDAPI
CCCCCCCCCHHHCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
YTIDYEQNLMAAQKNLKGIKDAKPSPAILFEDWSKLYVE
EEEECCHHHHHHHHHCCCCCCCCCCCEEEEECHHHHHCC
>Mature Secondary Structure
MKFKKLASLILVSSLMLTFTACASNDKDKSNGSKSGGTVVIPMASDPDIINGAFANVKED
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCCCCHHCCCCCH
SLASNIVYAPLYTYEKGNLVNYLAEKVDFKDSKELTIKLKSNLKWHDGKPITAEDVLFTF
HHHCCEEEEEEEEECCCCHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHH
NTVLDEKQNSPSRQYLLVGEKPVKVEKIDDLTVKITLPTASESFLYGISKISPIPKHVFE
HHHHHHCCCCCCCEEEEECCCCCEEEEECCEEEEEEECCCCCHHHHHHHHCCCCHHHHHC
GESNIAKSEKNNNPVGSGAFKFKEWKKGESIVFEKNADYFGGEPKADSIALKIIPNEASQ
CCCHHHHCCCCCCCCCCCCEECHHHCCCCEEEEECCCCCCCCCCCCCCEEEEEECCCCCH
EAALNNGEISLMKTSAEGYEKAKSNSNLQTYTYSEERLNYIVFNQNISNMANKEVRQALS
HHCCCCCCEEEEEECCHHHHHHCCCCCCEEEEEHHHCCEEEEEECCHHHHHHHHHHHHHH
YALNRNEMIESAYGKEGSVPAKSILVPEADFYTEEGVEGYDQDTNKAKDLLDKSGVKIDK
HHHCHHHHHHHHCCCCCCCCHHHEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEE
LKIGYNTGRFGHKNYALVAQQELKKIGIEAEIVPYESKAFFNILFSNSTECDMYVNGYAW
EEEECCCCCCCCCCEEEEEHHHHHHCCCCEEEEECCCCEEEEEEECCCCCCEEEECCEEE
GLEPNPYRGMFETGQYCNQTKYSNAEIDALWEKGFTELNKEKREEIYKQIQQDISKDAPI
CCCCCCCCCHHHCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
YTIDYEQNLMAAQKNLKGIKDAKPSPAILFEDWSKLYVE
EEEECCHHHHHHHHHCCCCCCCCCCCEEEEECHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]