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The map label for this gene is glnH [H]

Identifier: 126699383

GI number: 126699383

Start: 2055837

End: 2056640

Strand: Direct

Name: glnH [H]

Synonym: CD1774

Alternate gene names: 126699383

Gene position: 2055837-2056640 (Clockwise)

Preceding gene: 126699382

Following gene: 126699384

Centisome position: 47.92

GC content: 29.35

Gene sequence:

>804_bases
ATGAAAAATATATTAAAAAAAGTAGGAATATTTACAATTATGTTAGGTTTATTAGGTGGAGTTGTAGGTTGTTCAAAACC
AGATAATGAAAAAGATAAGGATGCTTCAAAGGAATCTAAAAAAGAAGTAGTTGTAGGGTTTGATAATACGTTTGTACCAA
TGGGATTTTTAGATGAAAAGGGAGATACAGTAGGATTTGACGTAGACTTAGCTAAAGAAACATTTAAAAGACTTGGAATG
GAAGTTAAGTTTCAGCCTATAGATTGGTCAATGAAGGAAACTGAATTAAATGATTCAAAAACTGTAGATGTATTATGGAA
TGGATATTCTATAACAGACGAAAGAAAGAAAATAGTATCATATACAGAACCATATTTACAAAATAAACAGATAATAGTTA
CTCTAAGTGACTCAAAAATAAATTCAAAAGCTGATTTAAAAGATAAAGAAGTTGGAACACAACAAGGTTCAACAGCACTT
GATGCAGTGGAAAAGGATAAAGATTTTATGAATAGCTTAAAAGGTGGAGCACCAGTTCTTTATGATACTTATGATAAAGC
ACTTAGAGATTTAGAGATAGGTAGAACTAGTGCAGTTGTTGGTGATGAAGTTTTAATTAGATACTATATGGGACAAAAGG
GTGAAGACAAATACAAGGTATTAAAAGACGATTTTGGATTAGAAGATTATGTTGTAGCAACTTCTAAAGAAAATCCAGAA
CTTTGTGAGAAAATAAATGAAACATTAAAAGAAATGAAAAAAGATGGAACTTTTGACAAAATTTATGATAAGTGGTTTAA
ATAG

Upstream 100 bases:

>100_bases
AATATAATTTTTTAAGCGCGCAGAGAATTATATTGCAATAGATACACTTTTTAAAAAATAATCAAAATATTTTAAAAATT
AAAAGACGAGGGAGAATATT

Downstream 100 bases:

>100_bases
AAGATAAGTTAGCTGTTAAGAAGGGGGCACTAAAGGCATGTTACAAGGTTTAGAGATAGTTATAGCAATGTTTTGTATAA
CATTGATAGTATCAATTCCA

Product: amino acid ABC transporter substrate-binding protein

Products: ADP; phosphate; L-glutamine [Cytoplasm] [C]

Alternate protein names: GlnBP [H]

Number of amino acids: Translated: 267; Mature: 267

Protein sequence:

>267_residues
MKNILKKVGIFTIMLGLLGGVVGCSKPDNEKDKDASKESKKEVVVGFDNTFVPMGFLDEKGDTVGFDVDLAKETFKRLGM
EVKFQPIDWSMKETELNDSKTVDVLWNGYSITDERKKIVSYTEPYLQNKQIIVTLSDSKINSKADLKDKEVGTQQGSTAL
DAVEKDKDFMNSLKGGAPVLYDTYDKALRDLEIGRTSAVVGDEVLIRYYMGQKGEDKYKVLKDDFGLEDYVVATSKENPE
LCEKINETLKEMKKDGTFDKIYDKWFK

Sequences:

>Translated_267_residues
MKNILKKVGIFTIMLGLLGGVVGCSKPDNEKDKDASKESKKEVVVGFDNTFVPMGFLDEKGDTVGFDVDLAKETFKRLGM
EVKFQPIDWSMKETELNDSKTVDVLWNGYSITDERKKIVSYTEPYLQNKQIIVTLSDSKINSKADLKDKEVGTQQGSTAL
DAVEKDKDFMNSLKGGAPVLYDTYDKALRDLEIGRTSAVVGDEVLIRYYMGQKGEDKYKVLKDDFGLEDYVVATSKENPE
LCEKINETLKEMKKDGTFDKIYDKWFK
>Mature_267_residues
MKNILKKVGIFTIMLGLLGGVVGCSKPDNEKDKDASKESKKEVVVGFDNTFVPMGFLDEKGDTVGFDVDLAKETFKRLGM
EVKFQPIDWSMKETELNDSKTVDVLWNGYSITDERKKIVSYTEPYLQNKQIIVTLSDSKINSKADLKDKEVGTQQGSTAL
DAVEKDKDFMNSLKGGAPVLYDTYDKALRDLEIGRTSAVVGDEVLIRYYMGQKGEDKYKVLKDDFGLEDYVVATSKENPE
LCEKINETLKEMKKDGTFDKIYDKWFK

Specific function: Involved in a glutamine-transport system glnHPQ [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787031, Length=237, Percent_Identity=31.6455696202532, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI1788228, Length=226, Percent_Identity=30.9734513274336, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1787085, Length=232, Percent_Identity=30.1724137931034, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1787088, Length=239, Percent_Identity=30.9623430962343, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1788649, Length=237, Percent_Identity=30.8016877637131, Blast_Score=88, Evalue=5e-19,
Organism=Escherichia coli, GI1788648, Length=239, Percent_Identity=30.5439330543933, Blast_Score=87, Evalue=9e-19,

Paralogues:

None

Copy number: 1440 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 460 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 30192; Mature: 30192

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNILKKVGIFTIMLGLLGGVVGCSKPDNEKDKDASKESKKEVVVGFDNTFVPMGFLDEK
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCEEEECCCCEEECCCCCCC
GDTVGFDVDLAKETFKRLGMEVKFQPIDWSMKETELNDSKTVDVLWNGYSITDERKKIVS
CCEEECCHHHHHHHHHHCCCEEEEECCCCCCHHCCCCCCCEEEEEECCEECCHHHHHHHH
YTEPYLQNKQIIVTLSDSKINSKADLKDKEVGTQQGSTALDAVEKDKDFMNSLKGGAPVL
HHHHHHCCCEEEEEECCCCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCEE
YDTYDKALRDLEIGRTSAVVGDEVLIRYYMGQKGEDKYKVLKDDFGLEDYVVATSKENPE
EHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCEEEEECCCCHH
LCEKINETLKEMKKDGTFDKIYDKWFK
HHHHHHHHHHHHHHCCCHHHHHHHHCC
>Mature Secondary Structure
MKNILKKVGIFTIMLGLLGGVVGCSKPDNEKDKDASKESKKEVVVGFDNTFVPMGFLDEK
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCEEEECCCCEEECCCCCCC
GDTVGFDVDLAKETFKRLGMEVKFQPIDWSMKETELNDSKTVDVLWNGYSITDERKKIVS
CCEEECCHHHHHHHHHHCCCEEEEECCCCCCHHCCCCCCCEEEEEECCEECCHHHHHHHH
YTEPYLQNKQIIVTLSDSKINSKADLKDKEVGTQQGSTALDAVEKDKDFMNSLKGGAPVL
HHHHHHCCCEEEEEECCCCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCEE
YDTYDKALRDLEIGRTSAVVGDEVLIRYYMGQKGEDKYKVLKDDFGLEDYVVATSKENPE
EHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCEEEEECCCCHH
LCEKINETLKEMKKDGTFDKIYDKWFK
HHHHHHHHHHHHHHCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamine [Periplasm]; H2O [C]

Specific reaction: ATP + L-glutamine [Periplasm] + H2O = ADP + phosphate + L-glutamine [Cytoplasm] [C]

General reaction: Translocates substrate across the membrane [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]