Click here to switch to the map view.

The map label for this gene is def1 [H]

Identifier: 126699364

GI number: 126699364

Start: 2034575

End: 2035030

Strand: Direct

Name: def1 [H]

Synonym: CD1756

Alternate gene names: 126699364

Gene position: 2034575-2035030 (Clockwise)

Preceding gene: 126699363

Following gene: 126699365

Centisome position: 47.42

GC content: 30.26

Gene sequence:

>456_bases
ATGGCTATAAGAAAGATAAGAACATTTGATGATGAAATTTTAAGAAAAAAGAGTAAGTATGTAGAAAACGTAGATAATAA
GATAAGAGAAATATTAAATGACATGGCAGAGACCATGTACAATACTCCAAATGGAGGAGGATTAGCTGCATGCCAAGTAG
GAGTATTAAAGCGATTAGTTGTTATAGATTTAGGAGAAGGACTTATTAAGCTTGTTAATCCTGAAATTATTAAACAAGAA
GGGGAACAAATTGTTGTTGAAGGATGTTTAAGTTTTCCTGAAGTATGGGGTAAATTAAAAAGACCAAAAAAAGTTACAGT
GCAAGCTCTAAACGAATACGGTGAGAAAATAGAAATTAAAGGTTCAGGTTTTATGGCAAAATGTCTTTGTCATGAGATAG
ACCATTTAAATGGTATAGTTTTTACAGATAAAATTATTGAACATGTAAAATTGTAA

Upstream 100 bases:

>100_bases
TATGATATTTCAAGAATTTTTAATGAACCAATTGAAAGTGTATTTTTATTTGAAGAAAGTGAAAGAAAATCAAGAGCACA
GATAAGTAGGGGAGAATATT

Downstream 100 bases:

>100_bases
GATAGATTGGTTATTGAATTTGTAAAATATTTTATAGTAAGGAGTTAAATTATGAGATTATGGCATAAGGATTTAATTGA
TGTATTACCTAAAAATCAAT

Product: peptide deformylase 1

Products: NA

Alternate protein names: PDF 2; Polypeptide deformylase 2 [H]

Number of amino acids: Translated: 151; Mature: 150

Protein sequence:

>151_residues
MAIRKIRTFDDEILRKKSKYVENVDNKIREILNDMAETMYNTPNGGGLAACQVGVLKRLVVIDLGEGLIKLVNPEIIKQE
GEQIVVEGCLSFPEVWGKLKRPKKVTVQALNEYGEKIEIKGSGFMAKCLCHEIDHLNGIVFTDKIIEHVKL

Sequences:

>Translated_151_residues
MAIRKIRTFDDEILRKKSKYVENVDNKIREILNDMAETMYNTPNGGGLAACQVGVLKRLVVIDLGEGLIKLVNPEIIKQE
GEQIVVEGCLSFPEVWGKLKRPKKVTVQALNEYGEKIEIKGSGFMAKCLCHEIDHLNGIVFTDKIIEHVKL
>Mature_150_residues
AIRKIRTFDDEILRKKSKYVENVDNKIREILNDMAETMYNTPNGGGLAACQVGVLKRLVVIDLGEGLIKLVNPEIIKQEG
EQIVVEGCLSFPEVWGKLKRPKKVTVQALNEYGEKIEIKGSGFMAKCLCHEIDHLNGIVFTDKIIEHVKL

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Escherichia coli, GI1789682, Length=146, Percent_Identity=41.7808219178082, Blast_Score=114, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24645728, Length=165, Percent_Identity=26.0606060606061, Blast_Score=64, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 17021; Mature: 16890

Theoretical pI: Translated: 7.95; Mature: 7.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIRKIRTFDDEILRKKSKYVENVDNKIREILNDMAETMYNTPNGGGLAACQVGVLKRLV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
VIDLGEGLIKLVNPEIIKQEGEQIVVEGCLSFPEVWGKLKRPKKVTVQALNEYGEKIEIK
HHHHCHHHHHHHCHHHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCEEEEC
GSGFMAKCLCHEIDHLNGIVFTDKIIEHVKL
CCCHHHHHHHHHHHHCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
AIRKIRTFDDEILRKKSKYVENVDNKIREILNDMAETMYNTPNGGGLAACQVGVLKRLV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
VIDLGEGLIKLVNPEIIKQEGEQIVVEGCLSFPEVWGKLKRPKKVTVQALNEYGEKIEIK
HHHHCHHHHHHHCHHHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCEEEEC
GSGFMAKCLCHEIDHLNGIVFTDKIIEHVKL
CCCHHHHHHHHHHHHCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]