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The map label for this gene is mtbC3 [H]

Identifier: 126699339

GI number: 126699339

Start: 2007160

End: 2007798

Strand: Direct

Name: mtbC3 [H]

Synonym: CD1732

Alternate gene names: 126699339

Gene position: 2007160-2007798 (Clockwise)

Preceding gene: 126699338

Following gene: 126699340

Centisome position: 46.78

GC content: 29.11

Gene sequence:

>639_bases
ATGGACATACTTGATGATATAGCAGAATGTATATTAAATATGGGTGTAGATAATATAGAGAATTTAGTAAAAATTGCTAT
AGATAAAAATATTGATGTGGAAGATATTTATGAATATGGTTTAAACAAAGGTATGATAGGAGCTTTAGATAAGTTTGAGA
ATAAGGAATACTATCTTTCAGAAGTTATAGTATGCACAGATGCATTAAATAAAGGTATTAATCTTTTGAAGGGAACTGGA
AAAGTAAAGAAAAAATCAAAGGGAGTAATTCTGATGTCTGTAGTAGAAGGAGATACTCATGAGATAGGAAAAAATATTGT
AAAAGTAATGGTAGAAGCCACTGGATATAAGGTTATTGACTTAGGTGTAAATAGAAAAAGTGAAGATATAATAGAAGAAG
CTATAAAAAACAATGTAGATATAATAGGCTTATCTTCAATGATGACAACTACAATGGAAAATATGAAATCGGTTATAGAC
GAACTTAATATGATTGAGATAGATAAAAGACCAAAAGTAATAATTGGTGGAGGTCCTGTAAGTATGGAGTTTGCAGAAGA
AATAGGAGCAGATGGATACAGTTCAAATGCTCCAAAAGCAGTTAAATTAATAAATAAACTTATAGGAGGTGAAGTTTAA

Upstream 100 bases:

>100_bases
TAAAATTGATTGATACCTAGAGTTAGTCCTTGTTAAATAAGAGAAATTATTGGATATTGAAAAAGCAATTTTGTAAGTAA
TTAGAAAGGATGAGTATTAT

Downstream 100 bases:

>100_bases
ATGTTTTCAGATATGACTTTACTTCAGTATATAAATAGTGAAGATAGAGGTTTTTTTCTGCCTGATATGGGTACAAATGG
ATTGTTCTTAATAAATAAAA

Product: corrinoid protein

Products: L-Methionine; 5,6,7,8-Tetrahydrofolate [C]

Alternate protein names: NA

Number of amino acids: Translated: 212; Mature: 212

Protein sequence:

>212_residues
MDILDDIAECILNMGVDNIENLVKIAIDKNIDVEDIYEYGLNKGMIGALDKFENKEYYLSEVIVCTDALNKGINLLKGTG
KVKKKSKGVILMSVVEGDTHEIGKNIVKVMVEATGYKVIDLGVNRKSEDIIEEAIKNNVDIIGLSSMMTTTMENMKSVID
ELNMIEIDKRPKVIIGGGPVSMEFAEEIGADGYSSNAPKAVKLINKLIGGEV

Sequences:

>Translated_212_residues
MDILDDIAECILNMGVDNIENLVKIAIDKNIDVEDIYEYGLNKGMIGALDKFENKEYYLSEVIVCTDALNKGINLLKGTG
KVKKKSKGVILMSVVEGDTHEIGKNIVKVMVEATGYKVIDLGVNRKSEDIIEEAIKNNVDIIGLSSMMTTTMENMKSVID
ELNMIEIDKRPKVIIGGGPVSMEFAEEIGADGYSSNAPKAVKLINKLIGGEV
>Mature_212_residues
MDILDDIAECILNMGVDNIENLVKIAIDKNIDVEDIYEYGLNKGMIGALDKFENKEYYLSEVIVCTDALNKGINLLKGTG
KVKKKSKGVILMSVVEGDTHEIGKNIVKVMVEATGYKVIDLGVNRKSEDIIEEAIKNNVDIIGLSSMMTTTMENMKSVID
ELNMIEIDKRPKVIIGGGPVSMEFAEEIGADGYSSNAPKAVKLINKLIGGEV

Specific function: Acts as a methyl group carrier between mtbB and mtbA [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 B12-binding N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI169790923, Length=141, Percent_Identity=33.3333333333333, Blast_Score=74, Evalue=1e-13,
Organism=Escherichia coli, GI1790450, Length=131, Percent_Identity=31.2977099236641, Blast_Score=74, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17535405, Length=104, Percent_Identity=36.5384615384615, Blast_Score=72, Evalue=2e-13,

Paralogues:

None

Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006158
- InterPro:   IPR003759
- InterPro:   IPR012741 [H]

Pfam domain/function: PF02310 B12-binding; PF02607 B12-binding_2 [H]

EC number: 2.1.1.13 [C]

Molecular weight: Translated: 23158; Mature: 23158

Theoretical pI: Translated: 4.44; Mature: 4.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDILDDIAECILNMGVDNIENLVKIAIDKNIDVEDIYEYGLNKGMIGALDKFENKEYYLS
CCHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCHHHHHHHCCCHHHHH
EVIVCTDALNKGINLLKGTGKVKKKSKGVILMSVVEGDTHEIGKNIVKVMVEATGYKVID
HHHHHHHHHHHCHHHHHCCCCCHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHCCEEEEE
LGVNRKSEDIIEEAIKNNVDIIGLSSMMTTTMENMKSVIDELNMIEIDKRPKVIIGGGPV
ECCCCCHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHCCEEEECCCCEEEECCCCC
SMEFAEEIGADGYSSNAPKAVKLINKLIGGEV
CHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure
MDILDDIAECILNMGVDNIENLVKIAIDKNIDVEDIYEYGLNKGMIGALDKFENKEYYLS
CCHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCHHHHHHHCCCHHHHH
EVIVCTDALNKGINLLKGTGKVKKKSKGVILMSVVEGDTHEIGKNIVKVMVEATGYKVID
HHHHHHHHHHHCHHHHHCCCCCHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHCCEEEEE
LGVNRKSEDIIEEAIKNNVDIIGLSSMMTTTMENMKSVIDELNMIEIDKRPKVIIGGGPV
ECCCCCHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHCCEEEECCCCEEEECCCCC
SMEFAEEIGADGYSSNAPKAVKLINKLIGGEV
CHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: ATP; Cobinamide; S-adenosyl-L-methionine [C]

Metal ions: Mg2+ [C]

Kcat value (1/min): 500-780 [C]

Specific activity: 3.8

Km value (mM): 0.035 {S-methyltetrahydrofolate}} 0.0016 {S-adenosyl-L-methionine}} 0.00065 {S-adenosyl-L-methionine}} 0.06 {methyltetrahydrofolate}} 0.03 {methyltetrahydrofolate}} 0.016 {homocysteine}} [C]

Substrates: 5-Methyltetrahydrofolate; L-Homocysteine [C]

Specific reaction: 5-Methyltetrahydrofolate + L-Homocysteine --> L-Methionine + 5,6,7,8-Tetrahydrofolate [C]

General reaction: Methyl group transfer [C]

Inhibitor: 5, 6-Dimethyl benzimidazole; alpha-Ribazole 3'-phosphate; Be2+; Cobinamide; Propyl iodide; Sr2+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]