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The map label for this gene is thiF [C]

Identifier: 126699310

GI number: 126699310

Start: 1977908

End: 1978711

Strand: Direct

Name: thiF [C]

Synonym: CD1703

Alternate gene names: 126699310

Gene position: 1977908-1978711 (Clockwise)

Preceding gene: 126699309

Following gene: 126699311

Centisome position: 46.1

GC content: 28.61

Gene sequence:

>804_bases
GTGGTTAAAATAAAAATATTTGTAAATGAATTATTAACAGATGTTGAGGAAAATACAACTGCTTATAAAGTTAGAGATAG
CTATAACAATAAATGTGATGTATTGGTGTTAAATGGATATCCAATTAAAACAGATATGACCCTTTGTGAAAATGATAAAT
TAACACTTATACAAAAAGGAGTTAAGCCATCACTTGATGAATTGGAAAGCTTGTTGATTGCTAGACATACTCCAAATATA
CATAATAAATTAAAAAAAGGGAAAGTTGCTATATTGGGATTGGGGGGACTTGGTTCCAATATAGCTATCTCTTTAGCTAG
AATAGGAGTGGGAGAACTTTTACTTATAGATTATGATGTTGTAGAACCATCTAATTTAAATAGACAGCAGTATTTTATAG
ATGATATAGGAAAACTAAAAACTGATGCCATGATAGAAAATATAAAAAAAATAAATCCATTTATAAAATTAAATAAACGA
GATATTTTTTTAAACAAGAACAATATGGATACTATTAAGGATTCAGATTTAATAATTGAGGCCTTTGATGATGCTTCATG
TAAAGCACAGGTATGTAATTATGTATTAATAAATTTAAAAGATAAATATTTAATAGCATCATCTGGTATGGCAGGTTACT
ATGATTCAAATATAATAACTACTAAGAAGATAAAAGACAGGTTTTATATATGTGGGGATTTTGTGAATGAAGCTAAATTT
GGTGAAGGGCTTATGGCTCCAAGGGTTGCCATCTGCGCAAATCATATGGCAAATTTGGCAACGCAAATACTTATAGATAT
GTAA

Upstream 100 bases:

>100_bases
GTTGTAGTTGAAGTGAATTTAGAGATAATTGAAGAATCTAACTACAATACATATGTATTAAAAGACGAGGACATTGTAGA
GCTTATTAGCTTTATTGGAG

Downstream 100 bases:

>100_bases
ATATTAAAATTAGATATAAAAAAGATTGAATATTAAAAAAATTTAACGTGGAGGGAAATAATATGGATAAATTGGTACTT
GGTGGACATGAATTTAATAG

Product: thiamine biosynthesis protein

Products: ThiS-COSH; AMP; ThiS-COAMP; pyrophosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 267; Mature: 267

Protein sequence:

>267_residues
MVKIKIFVNELLTDVEENTTAYKVRDSYNNKCDVLVLNGYPIKTDMTLCENDKLTLIQKGVKPSLDELESLLIARHTPNI
HNKLKKGKVAILGLGGLGSNIAISLARIGVGELLLIDYDVVEPSNLNRQQYFIDDIGKLKTDAMIENIKKINPFIKLNKR
DIFLNKNNMDTIKDSDLIIEAFDDASCKAQVCNYVLINLKDKYLIASSGMAGYYDSNIITTKKIKDRFYICGDFVNEAKF
GEGLMAPRVAICANHMANLATQILIDM

Sequences:

>Translated_267_residues
MVKIKIFVNELLTDVEENTTAYKVRDSYNNKCDVLVLNGYPIKTDMTLCENDKLTLIQKGVKPSLDELESLLIARHTPNI
HNKLKKGKVAILGLGGLGSNIAISLARIGVGELLLIDYDVVEPSNLNRQQYFIDDIGKLKTDAMIENIKKINPFIKLNKR
DIFLNKNNMDTIKDSDLIIEAFDDASCKAQVCNYVLINLKDKYLIASSGMAGYYDSNIITTKKIKDRFYICGDFVNEAKF
GEGLMAPRVAICANHMANLATQILIDM
>Mature_267_residues
MVKIKIFVNELLTDVEENTTAYKVRDSYNNKCDVLVLNGYPIKTDMTLCENDKLTLIQKGVKPSLDELESLLIARHTPNI
HNKLKKGKVAILGLGGLGSNIAISLARIGVGELLLIDYDVVEPSNLNRQQYFIDDIGKLKTDAMIENIKKINPFIKLNKR
DIFLNKNNMDTIKDSDLIIEAFDDASCKAQVCNYVLINLKDKYLIASSGMAGYYDSNIITTKKIKDRFYICGDFVNEAKF
GEGLMAPRVAICANHMANLATQILIDM

Specific function: Catalyzes The Adenylation By ATP Of The Carboxyl- Terminal Glycine Of This. [C]

COG id: COG0476

COG function: function code H; Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 2

Gene ontology:

Cell location: Membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hesA/moeB/thiF family [H]

Homologues:

Organism=Escherichia coli, GI87082356, Length=153, Percent_Identity=30.0653594771242, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1789177, Length=134, Percent_Identity=32.8358208955224, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787048, Length=113, Percent_Identity=33.6283185840708, Blast_Score=66, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6321903, Length=131, Percent_Identity=35.1145038167939, Blast_Score=63, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009036
- InterPro:   IPR016040
- InterPro:   IPR000594 [H]

Pfam domain/function: PF00899 ThiF [H]

EC number: 2.7.7.- [C]

Molecular weight: Translated: 29902; Mature: 29902

Theoretical pI: Translated: 7.30; Mature: 7.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKIKIFVNELLTDVEENTTAYKVRDSYNNKCDVLVLNGYPIKTDMTLCENDKLTLIQKG
CEEEEEEHHHHHHHHHCCCEEEEEECCCCCCCCEEEECCEEEECCCEEECCCCEEEEECC
VKPSLDELESLLIARHTPNIHNKLKKGKVAILGLGGLGSNIAISLARIGVGELLLIDYDV
CCCCHHHHHHHHHHCCCCCHHHHHCCCCEEEEEECCCCCCCEEEEECCCCCEEEEEEEEE
VEPSNLNRQQYFIDDIGKLKTDAMIENIKKINPFIKLNKRDIFLNKNNMDTIKDSDLIIE
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCEEEEEECCCCCCCCCCCEEEE
AFDDASCKAQVCNYVLINLKDKYLIASSGMAGYYDSNIITTKKIKDRFYICGDFVNEAKF
ECCCCCHHHHHCCEEEEEECCCEEEEECCCCCEECCCEEEEEECCCEEEEEECHHHHHHC
GEGLMAPRVAICANHMANLATQILIDM
CCCCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MVKIKIFVNELLTDVEENTTAYKVRDSYNNKCDVLVLNGYPIKTDMTLCENDKLTLIQKG
CEEEEEEHHHHHHHHHCCCEEEEEECCCCCCCCEEEECCEEEECCCEEECCCCEEEEECC
VKPSLDELESLLIARHTPNIHNKLKKGKVAILGLGGLGSNIAISLARIGVGELLLIDYDV
CCCCHHHHHHHHHHCCCCCHHHHHCCCCEEEEEECCCCCCCEEEEECCCCCEEEEEEEEE
VEPSNLNRQQYFIDDIGKLKTDAMIENIKKINPFIKLNKRDIFLNKNNMDTIKDSDLIIE
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCEEEEEECCCCCCCCCCCEEEE
AFDDASCKAQVCNYVLINLKDKYLIASSGMAGYYDSNIITTKKIKDRFYICGDFVNEAKF
ECCCCCHHHHHCCEEEEEECCCEEEEECCCCCEECCCEEEEEECCCEEEEEECHHHHHHC
GEGLMAPRVAICANHMANLATQILIDM
CCCCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ThiS-COAMP; L-cysteine; ThiS protein; ATP [C]

Specific reaction: ThiS-COAMP + L-cysteine = ThiS-COSH + AMP ThiS protein + ATP = ThiS-COAMP + pyrophosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]