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The map label for this gene is hisF

Identifier: 126699157

GI number: 126699157

Start: 1800363

End: 1801121

Strand: Direct

Name: hisF

Synonym: CD1553

Alternate gene names: 126699157

Gene position: 1800363-1801121 (Clockwise)

Preceding gene: 126699156

Following gene: 126699158

Centisome position: 41.96

GC content: 31.49

Gene sequence:

>759_bases
ATGCTTACTAGGAGAATAATACCTTGTCTAGATGTGAGAAATGGAAGAGTTGTAAAGGGGAAAAAATTTAAAGATATAGT
AGATGTTGATAGTCCAGAAGTACTTGGAAAATTTTATAGTGATTGTGGTGCAGATGAACTTGTATTCTATGATATAACTG
CATCAAATGAAGAGAGAAAGACATCTTTAGAATTTGTGACAAAAGTTGCAGAAAATATAAATATACCATTTTGTGTTGGT
GGAGGAGTAAATAAATTAGAAGATTTCACTGATATTTTAAGAAAAGGTGCTGATAAAGTTTCTATAAATTCATCAGCAGT
CAAAAATCCTGAATTAATAAGGGAAGCTTCATTAAAATTTGGAGCACAATGTGTTGTTTTATCGATAGATGCAAAGAAAA
ATGAAGAGGGTTCATGGAGCGTATACGTAAAAGGTGGAAGAGAAAAAACTAATCTGGATGCAATTGAATGGGCTGTTAAA
GGTGTAGAACTTGGAGCAGGAGAAATCGTTGTAAATAGTATGGATGAAGATGGAATGAAAAATGGATATGATATAGAATT
ATTATCAAAGATAACTTCTTTAGTAAATGTACCTGTTATAGCATCTGGAGGCGCTGGAAAAAAGGAAGATTTTTATGAGG
CGGTTAATAAATCCAATGTAGATGGAATTTTAGCAGCATCTGTTTTTCATTTTGGCGAGATAAAAATAAACGATTTAAAG
AAATATTTAAAAGACATGGGTGTAGAAGTAAGATTATAA

Upstream 100 bases:

>100_bases
TAGAAGACGTAAAAAGACTAAAAGCTATGAACTTATATGGTGCAATAATAGGTAAAGCGCTTTATGATAAAAAGATAGAC
TTTAAGGAGGCACAACAATT

Downstream 100 bases:

>100_bases
GTTAAATACTAAAAGGAGATTAGAAATATGGATAATAAGTGTAACAATGTATACAGTGATGAAGTAGAGAAATTTATAAG
AAGTATAAAGTTTGATGATA

Product: imidazole glycerol phosphate synthase subunit

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MLTRRIIPCLDVRNGRVVKGKKFKDIVDVDSPEVLGKFYSDCGADELVFYDITASNEERKTSLEFVTKVAENINIPFCVG
GGVNKLEDFTDILRKGADKVSINSSAVKNPELIREASLKFGAQCVVLSIDAKKNEEGSWSVYVKGGREKTNLDAIEWAVK
GVELGAGEIVVNSMDEDGMKNGYDIELLSKITSLVNVPVIASGGAGKKEDFYEAVNKSNVDGILAASVFHFGEIKINDLK
KYLKDMGVEVRL

Sequences:

>Translated_252_residues
MLTRRIIPCLDVRNGRVVKGKKFKDIVDVDSPEVLGKFYSDCGADELVFYDITASNEERKTSLEFVTKVAENINIPFCVG
GGVNKLEDFTDILRKGADKVSINSSAVKNPELIREASLKFGAQCVVLSIDAKKNEEGSWSVYVKGGREKTNLDAIEWAVK
GVELGAGEIVVNSMDEDGMKNGYDIELLSKITSLVNVPVIASGGAGKKEDFYEAVNKSNVDGILAASVFHFGEIKINDLK
KYLKDMGVEVRL
>Mature_252_residues
MLTRRIIPCLDVRNGRVVKGKKFKDIVDVDSPEVLGKFYSDCGADELVFYDITASNEERKTSLEFVTKVAENINIPFCVG
GGVNKLEDFTDILRKGADKVSINSSAVKNPELIREASLKFGAQCVVLSIDAKKNEEGSWSVYVKGGREKTNLDAIEWAVK
GVELGAGEIVVNSMDEDGMKNGYDIELLSKITSLVNVPVIASGGAGKKEDFYEAVNKSNVDGILAASVFHFGEIKINDLK
KYLKDMGVEVRL

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI1788336, Length=257, Percent_Identity=49.4163424124514, Blast_Score=239, Evalue=1e-64,
Organism=Escherichia coli, GI87082028, Length=240, Percent_Identity=25.8333333333333, Blast_Score=88, Evalue=7e-19,
Organism=Saccharomyces cerevisiae, GI6319725, Length=314, Percent_Identity=34.7133757961783, Blast_Score=154, Evalue=1e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS6_CLOD6 (Q18C74)

Other databases:

- EMBL:   AM180355
- RefSeq:   YP_001088054.1
- ProteinModelPortal:   Q18C74
- SMR:   Q18C74
- STRING:   Q18C74
- GeneID:   4913344
- GenomeReviews:   AM180355_GR
- KEGG:   cdf:CD1553
- NMPDR:   fig|1496.1.peg.3365
- eggNOG:   COG0107
- HOGENOM:   HBG541613
- OMA:   RVVKGTN
- ProtClustDB:   CLSK2534933
- GO:   GO:0005737
- HAMAP:   MF_01013
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00735

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: 4.1.3.-

Molecular weight: Translated: 27577; Mature: 27577

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: NA

Important sites: ACT_SITE 11-11 ACT_SITE 130-130

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTRRIIPCLDVRNGRVVKGKKFKDIVDVDSPEVLGKFYSDCGADELVFYDITASNEERK
CCCCCCCCCEECCCCEEECCCCCCHHHCCCCHHHHHHHHHCCCCCCEEEEEEECCCCHHH
TSLEFVTKVAENINIPFCVGGGVNKLEDFTDILRKGADKVSINSSAVKNPELIREASLKF
HHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHCC
GAQCVVLSIDAKKNEEGSWSVYVKGGREKTNLDAIEWAVKGVELGAGEIVVNSMDEDGMK
CCEEEEEEEECCCCCCCCEEEEEECCCCCCCCHHHHHHHHCEEECCCEEEEECCCCCCCC
NGYDIELLSKITSLVNVPVIASGGAGKKEDFYEAVNKSNVDGILAASVFHFGEIKINDLK
CCCCHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHCCCCCCHHHHHHHHHCCCEEHHHHH
KYLKDMGVEVRL
HHHHHCCCEECC
>Mature Secondary Structure
MLTRRIIPCLDVRNGRVVKGKKFKDIVDVDSPEVLGKFYSDCGADELVFYDITASNEERK
CCCCCCCCCEECCCCEEECCCCCCHHHCCCCHHHHHHHHHCCCCCCEEEEEEECCCCHHH
TSLEFVTKVAENINIPFCVGGGVNKLEDFTDILRKGADKVSINSSAVKNPELIREASLKF
HHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHCC
GAQCVVLSIDAKKNEEGSWSVYVKGGREKTNLDAIEWAVKGVELGAGEIVVNSMDEDGMK
CCEEEEEEEECCCCCCCCEEEEEECCCCCCCCHHHHHHHHCEEECCCEEEEECCCCCCCC
NGYDIELLSKITSLVNVPVIASGGAGKKEDFYEAVNKSNVDGILAASVFHFGEIKINDLK
CCCCHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHCCCCCCHHHHHHHHHCCCEEHHHHH
KYLKDMGVEVRL
HHHHHCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA