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The map label for this gene is dgoA [C]

Identifier: 126698991

GI number: 126698991

Start: 1607849

End: 1608916

Strand: Direct

Name: dgoA [C]

Synonym: CD1389

Alternate gene names: 126698991

Gene position: 1607849-1608916 (Clockwise)

Preceding gene: 126698990

Following gene: 126698992

Centisome position: 37.48

GC content: 30.99

Gene sequence:

>1068_bases
ATGAAGATAACAGATATCAAATTTGAAAAGCTTAGAATTAAATTAAAAAAACCTGTAGTAGTTTCATTTGGAGTAATTGA
GTATGGAGAAAGTATAATTTTAAAAATTGAAACTGATGAAGGGTATTGTGGATTTGGCGAAGCAGCACCTCTTGCAGCAG
TTACAGGTGAAGTTTTGGATAATGTTCTTTCAGTATTACTTATGTTTAAAAAAGAGTTAATAGGCAAAGACCCACTAGAT
ATTGAAACCATACATACAATCATGGATGGAATTATTATAGGTAATACTTCTGCAAAAGCAGCTATTGACATAGCTTTATA
TGATATTAAAGGAAAAATTATGAATGCACCCCTTTATAAAGTTCTTGGAGGATTTGATAGTAAAGTCCAAACGGATATTA
CTATAAGTATAGATAAGCCAGAAAAAATGGCAGTAGAAGCTTTAGAAAGAGTAAAAGAAGGTTTTAGGATATTGAAGCTT
AAAGCAGGTATAAATCCTGAAGACGATATTGAAGCTGTAAAGTTAATTAGAGAAGCCGTTGGAGAGAGTATAAGAATTAG
AATTGATGCAAATCAAGGATGGAACGTAAATAGCAGTATAAATACTATCAAAAAGTTAGAAGAATTTAATGTTGATGCAA
TTGAACAAGCACTTCCACATTGGGATTTAGATGGTACTGCTTACATAAGAAATAAAAGTAACACAAAAATTATGATAGAT
GAATCATTACATTCACCAATAGATGCAATTAAGGCTATAAAAAAGAATGCAGTTGATACATTTAATATTAAGCTGATGAA
ATCAAGTGGAATATATCCTGCTATAAAAATCAACAACATAGCTGAGGCAAGTGGAGTAAATTGTATGTTAGGATGTATGC
TGGAAACGAGAATTGGTATAACAGCAGCAGCAAATTTAATAGCATCTAAAAAGAACATAACAGAAGCTGACCTTGATAGT
TTTATGTTTTGTGAAGAATTAGAAGGTATATCTGGAGGATTTGTAATGGATGGAGATATTATGAATCTTGTCAATAAACC
AGGTTTAGGAATAGAAGTAAACTTATGA

Upstream 100 bases:

>100_bases
TTCAGTAAATTATCTTGACATATATATTGAAGTAATAATATAATTTAAAGTATAAAATTTTAAAAATTGAATTATAACTT
CAAAAAATAAGGAGGGTAAT

Downstream 100 bases:

>100_bases
TAAATTTGGAGGTTAAATATGAATACACTTTTTTCAAATCCAGGTAGTATACTTGCTGTAATGACATCAATGATTGCATT
GGGTTTTTACTTACAAAGAT

Product: chloromuconate cycloisomerase

Products: Glyceraldehyde 3-phosphate; Pyruvate [C]

Alternate protein names: AE epimerase; AEE [H]

Number of amino acids: Translated: 355; Mature: 355

Protein sequence:

>355_residues
MKITDIKFEKLRIKLKKPVVVSFGVIEYGESIILKIETDEGYCGFGEAAPLAAVTGEVLDNVLSVLLMFKKELIGKDPLD
IETIHTIMDGIIIGNTSAKAAIDIALYDIKGKIMNAPLYKVLGGFDSKVQTDITISIDKPEKMAVEALERVKEGFRILKL
KAGINPEDDIEAVKLIREAVGESIRIRIDANQGWNVNSSINTIKKLEEFNVDAIEQALPHWDLDGTAYIRNKSNTKIMID
ESLHSPIDAIKAIKKNAVDTFNIKLMKSSGIYPAIKINNIAEASGVNCMLGCMLETRIGITAAANLIASKKNITEADLDS
FMFCEELEGISGGFVMDGDIMNLVNKPGLGIEVNL

Sequences:

>Translated_355_residues
MKITDIKFEKLRIKLKKPVVVSFGVIEYGESIILKIETDEGYCGFGEAAPLAAVTGEVLDNVLSVLLMFKKELIGKDPLD
IETIHTIMDGIIIGNTSAKAAIDIALYDIKGKIMNAPLYKVLGGFDSKVQTDITISIDKPEKMAVEALERVKEGFRILKL
KAGINPEDDIEAVKLIREAVGESIRIRIDANQGWNVNSSINTIKKLEEFNVDAIEQALPHWDLDGTAYIRNKSNTKIMID
ESLHSPIDAIKAIKKNAVDTFNIKLMKSSGIYPAIKINNIAEASGVNCMLGCMLETRIGITAAANLIASKKNITEADLDS
FMFCEELEGISGGFVMDGDIMNLVNKPGLGIEVNL
>Mature_355_residues
MKITDIKFEKLRIKLKKPVVVSFGVIEYGESIILKIETDEGYCGFGEAAPLAAVTGEVLDNVLSVLLMFKKELIGKDPLD
IETIHTIMDGIIIGNTSAKAAIDIALYDIKGKIMNAPLYKVLGGFDSKVQTDITISIDKPEKMAVEALERVKEGFRILKL
KAGINPEDDIEAVKLIREAVGESIRIRIDANQGWNVNSSINTIKKLEEFNVDAIEQALPHWDLDGTAYIRNKSNTKIMID
ESLHSPIDAIKAIKKNAVDTFNIKLMKSSGIYPAIKINNIAEASGVNCMLGCMLETRIGITAAANLIASKKNITEADLDS
FMFCEELEGISGGFVMDGDIMNLVNKPGLGIEVNL

Specific function: Catalyzes the epimerization of L-Ala-D-Glu to L-Ala-L- Glu and has probably a role in the metabolism of the murein peptide, of which L-Ala-D-Glu is a component. Is also able to catalyze the epimerization of L-Ala-D-Asp [H]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI48994953, Length=351, Percent_Identity=26.4957264957265, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI87081876, Length=230, Percent_Identity=31.7391304347826, Blast_Score=83, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: 4.1.2.21; 4.2.1.6 [C]

Molecular weight: Translated: 38831; Mature: 38831

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKITDIKFEKLRIKLKKPVVVSFGVIEYGESIILKIETDEGYCGFGEAAPLAAVTGEVLD
CCCCCEEHEEEEEEECCCEEEEECHHHCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHH
NVLSVLLMFKKELIGKDPLDIETIHTIMDGIIIGNTSAKAAIDIALYDIKGKIMNAPLYK
HHHHHHHHHHHHHCCCCCCCHHHHHHHHCCEEECCCCCCEEEEEEEEEECCEEECCCHHH
VLGGFDSKVQTDITISIDKPEKMAVEALERVKEGFRILKLKAGINPEDDIEAVKLIREAV
HHCCCCCCEEEEEEEEECCCHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHC
GESIRIRIDANQGWNVNSSINTIKKLEEFNVDAIEQALPHWDLDGTAYIRNKSNTKIMID
CCEEEEEEECCCCCCCCCHHHHHHHHHHCCHHHHHHHCCCCCCCCEEEEECCCCCEEEEE
ESLHSPIDAIKAIKKNAVDTFNIKLMKSSGIYPAIKINNIAEASGVNCMLGCMLETRIGI
CCCCCHHHHHHHHHHCCCCEEEEEEEECCCCCEEEEECCCCCCCCCCEEEHHHHHHHCCH
TAAANLIASKKNITEADLDSFMFCEELEGISGGFVMDGDIMNLVNKPGLGIEVNL
HHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCEEECCHHHHHHCCCCCCEEEEC
>Mature Secondary Structure
MKITDIKFEKLRIKLKKPVVVSFGVIEYGESIILKIETDEGYCGFGEAAPLAAVTGEVLD
CCCCCEEHEEEEEEECCCEEEEECHHHCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHH
NVLSVLLMFKKELIGKDPLDIETIHTIMDGIIIGNTSAKAAIDIALYDIKGKIMNAPLYK
HHHHHHHHHHHHHCCCCCCCHHHHHHHHCCEEECCCCCCEEEEEEEEEECCEEECCCHHH
VLGGFDSKVQTDITISIDKPEKMAVEALERVKEGFRILKLKAGINPEDDIEAVKLIREAV
HHCCCCCCEEEEEEEEECCCHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHC
GESIRIRIDANQGWNVNSSINTIKKLEEFNVDAIEQALPHWDLDGTAYIRNKSNTKIMID
CCEEEEEEECCCCCCCCCHHHHHHHHHHCCHHHHHHHCCCCCCCCEEEEECCCCCEEEEE
ESLHSPIDAIKAIKKNAVDTFNIKLMKSSGIYPAIKINNIAEASGVNCMLGCMLETRIGI
CCCCCHHHHHHHHHHCCCCEEEEEEEECCCCCEEEEECCCCCCCCCCEEEHHHHHHHCCH
TAAANLIASKKNITEADLDSFMFCEELEGISGGFVMDGDIMNLVNKPGLGIEVNL
HHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCEEECCHHHHHHCCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-Dehydro-3-deoxy-D-galactonate 6-phosphate [C]

Specific reaction: 2-Dehydro-3-deoxy-D-galactonate 6-phosphate <==> Glyceraldehyde 3-phosphate + Pyruvate [C]

General reaction: elimination of H2O C-O bond; Cleavage [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]