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The map label for this gene is spaK [H]

Identifier: 126698951

GI number: 126698951

Start: 1568072

End: 1569460

Strand: Direct

Name: spaK [H]

Synonym: CD1352

Alternate gene names: 126698951

Gene position: 1568072-1569460 (Clockwise)

Preceding gene: 126698950

Following gene: 126698953

Centisome position: 36.55

GC content: 25.13

Gene sequence:

>1389_bases
ATGCTTGGAGGTAAAAAATCTAACAACAAAAAAGTTGAAAAGTTAAGAACTATTTTTATAAAATATTTATCTTTATTCTT
TATTATGACAATTTCAATTGTACTCTTTTTAATGTTATCATTTTCTGTATTATTATCATCAGGTGTAATTTTACCAGCTA
ATTATGCAGAAAAACAATTTAATAAATATAAAGAACAGATTATTTCAAGTGAAAAAGTAACTGAAGATATAATTCCAAGT
ATATATGAGTATGGGGTTTATACTTTAGATGGGAATTTAATTTCAGGTACCTTTAATAAAAAAGAATCAAAAGAAGTATG
GAATTTAATGAGAGATATTGAAGAAAGGCATGCATATTCAGAAAGTTATATAAAGTTTTTTAAGAAAGATGAAGTTTTTA
TAATAAAATACAAAATAGTATCAGAGTATAGTTCTCCAATTTTAAGAGCATATTTACCAAAACCAGAGACATTGGGTATG
ATTATTTTTAGTATAATATTTTTTATTGAAATTGTTATATTATCAAAAGTATTTGGCAAGAAATTTAATATTGAAATGGA
GCTTTTAAAAAATACTACAGAAAAAATAGAACAACAGGATTTAGATTTTGTTGTAGAATCTAGTAAAATTTATGAAATTA
ATAATGTCCTTTTTTCTATGGATAAAATGAAGTTGGCGTTAAAAGATTCTTTAGAAAAACAATGGATGCTAGAAGAAAAT
AGAAAGGAACAGATATCTGCACTGGCACATGATATAAAAACACCACTAACAATTATACATGGAAATACTGACTTATTAAT
TGAAATAAATGAAAATCCTGAGTTATTAGAATACATGGAATATATCTCAAAGGGTGTCACTCAAATAGAAAAATATATTA
ATACTTTAATAGATATATCAAAAACAGAGAATGGCTATATATTAAATAAAGAAGCAATCAGTATGAGTGAGTTTATAAAA
GATGTCTTAGTGCAAATAGAAGCATTAGCTAGTACTAAAAACTTAGATGTGGAGTTTTATAAACAAGATAATTTGCCTAA
AAATATAACTATAGATAGAGAACTGTTATTCAGAGCTATTATGAATATAATTTCAAATGCTATAGATTATTCACCAAGCC
AGAGTAAACTATACATAAGTGTATCAGTATGTAATAAATACTTAAAATTTGTAGTTACAGATTGTGGAAGTGGTTTTTCT
AAAGCAGATTTGTCAAAAGCTACAGAACAGTTTTATACAGGAGATTTGAGTAGAAATTCTAAATCTCATTATGGAATGGG
ACTCTATATTGTAAATAATATTGTACAAAAACATAATGGGATTTTATATATTGAAAATTCTGTGAAAACTGGTGGTGCTA
TGGTAACAATTGAAATACCAATAGTTTAG

Upstream 100 bases:

>100_bases
TAAAATATAACTTAGCAATAAAAAGATATAAAATAATCAAATAAAAAACTGGTTGGGTATTAATTATGAATTAATTAAAC
TATTGGAGGACAGGGTACAA

Downstream 100 bases:

>100_bases
TAAATAATATCCATTTTGTAAATACCTAAATTTTATGATTTATAGGTATTTATAAAATGGATAGTTTTTTTGACTTTATA
TATTAGTTAAATCTGATAAA

Product: lantibiotic two-component sensor histidine kinase

Products: NA

Alternate protein names: Subtilin biosynthesis sensor protein spaK [H]

Number of amino acids: Translated: 462; Mature: 462

Protein sequence:

>462_residues
MLGGKKSNNKKVEKLRTIFIKYLSLFFIMTISIVLFLMLSFSVLLSSGVILPANYAEKQFNKYKEQIISSEKVTEDIIPS
IYEYGVYTLDGNLISGTFNKKESKEVWNLMRDIEERHAYSESYIKFFKKDEVFIIKYKIVSEYSSPILRAYLPKPETLGM
IIFSIIFFIEIVILSKVFGKKFNIEMELLKNTTEKIEQQDLDFVVESSKIYEINNVLFSMDKMKLALKDSLEKQWMLEEN
RKEQISALAHDIKTPLTIIHGNTDLLIEINENPELLEYMEYISKGVTQIEKYINTLIDISKTENGYILNKEAISMSEFIK
DVLVQIEALASTKNLDVEFYKQDNLPKNITIDRELLFRAIMNIISNAIDYSPSQSKLYISVSVCNKYLKFVVTDCGSGFS
KADLSKATEQFYTGDLSRNSKSHYGMGLYIVNNIVQKHNGILYIENSVKTGGAMVTIEIPIV

Sequences:

>Translated_462_residues
MLGGKKSNNKKVEKLRTIFIKYLSLFFIMTISIVLFLMLSFSVLLSSGVILPANYAEKQFNKYKEQIISSEKVTEDIIPS
IYEYGVYTLDGNLISGTFNKKESKEVWNLMRDIEERHAYSESYIKFFKKDEVFIIKYKIVSEYSSPILRAYLPKPETLGM
IIFSIIFFIEIVILSKVFGKKFNIEMELLKNTTEKIEQQDLDFVVESSKIYEINNVLFSMDKMKLALKDSLEKQWMLEEN
RKEQISALAHDIKTPLTIIHGNTDLLIEINENPELLEYMEYISKGVTQIEKYINTLIDISKTENGYILNKEAISMSEFIK
DVLVQIEALASTKNLDVEFYKQDNLPKNITIDRELLFRAIMNIISNAIDYSPSQSKLYISVSVCNKYLKFVVTDCGSGFS
KADLSKATEQFYTGDLSRNSKSHYGMGLYIVNNIVQKHNGILYIENSVKTGGAMVTIEIPIV
>Mature_462_residues
MLGGKKSNNKKVEKLRTIFIKYLSLFFIMTISIVLFLMLSFSVLLSSGVILPANYAEKQFNKYKEQIISSEKVTEDIIPS
IYEYGVYTLDGNLISGTFNKKESKEVWNLMRDIEERHAYSESYIKFFKKDEVFIIKYKIVSEYSSPILRAYLPKPETLGM
IIFSIIFFIEIVILSKVFGKKFNIEMELLKNTTEKIEQQDLDFVVESSKIYEINNVLFSMDKMKLALKDSLEKQWMLEEN
RKEQISALAHDIKTPLTIIHGNTDLLIEINENPELLEYMEYISKGVTQIEKYINTLIDISKTENGYILNKEAISMSEFIK
DVLVQIEALASTKNLDVEFYKQDNLPKNITIDRELLFRAIMNIISNAIDYSPSQSKLYISVSVCNKYLKFVVTDCGSGFS
KADLSKATEQFYTGDLSRNSKSHYGMGLYIVNNIVQKHNGILYIENSVKTGGAMVTIEIPIV

Specific function: Member of the two-component regulatory system spaK/spaR involved in the regulation of the biosynthesis of lantibiotic subtilin. SpaK may function as a membrane-associated protein kinase that phosphorylates spaR in response to environmental signals [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1786912, Length=269, Percent_Identity=26.3940520446097, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1788393, Length=239, Percent_Identity=26.3598326359833, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1786783, Length=305, Percent_Identity=21.3114754098361, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1786600, Length=260, Percent_Identity=24.6153846153846, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1790346, Length=277, Percent_Identity=27.4368231046931, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1790861, Length=255, Percent_Identity=25.0980392156863, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1788279, Length=288, Percent_Identity=25, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1790436, Length=232, Percent_Identity=24.1379310344828, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1788549, Length=220, Percent_Identity=25, Blast_Score=72, Evalue=8e-14,
Organism=Escherichia coli, GI48994928, Length=241, Percent_Identity=24.896265560166, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR008358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 53126; Mature: 53126

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLGGKKSNNKKVEKLRTIFIKYLSLFFIMTISIVLFLMLSFSVLLSSGVILPANYAEKQF
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCHHHHHH
NKYKEQIISSEKVTEDIIPSIYEYGVYTLDGNLISGTFNKKESKEVWNLMRDIEERHAYS
HHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCEEECCCCCHHHHHHHHHHHHHHHHHHHH
ESYIKFFKKDEVFIIKYKIVSEYSSPILRAYLPKPETLGMIIFSIIFFIEIVILSKVFGK
HHHHHHHCCCCEEEEEEEEHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
KFNIEMELLKNTTEKIEQQDLDFVVESSKIYEINNVLFSMDKMKLALKDSLEKQWMLEEN
CCCCHHHHHHHHHHHHHHHCHHHEECCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RKEQISALAHDIKTPLTIIHGNTDLLIEINENPELLEYMEYISKGVTQIEKYINTLIDIS
HHHHHHHHHHHHCCCEEEEECCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
KTENGYILNKEAISMSEFIKDVLVQIEALASTKNLDVEFYKQDNLPKNITIDRELLFRAI
CCCCCEEECHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCEECHHHHHHHH
MNIISNAIDYSPSQSKLYISVSVCNKYLKFVVTDCGSGFSKADLSKATEQFYTGDLSRNS
HHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCC
KSHYGMGLYIVNNIVQKHNGILYIENSVKTGGAMVTIEIPIV
CCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCEEEEEEECCC
>Mature Secondary Structure
MLGGKKSNNKKVEKLRTIFIKYLSLFFIMTISIVLFLMLSFSVLLSSGVILPANYAEKQF
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCHHHHHH
NKYKEQIISSEKVTEDIIPSIYEYGVYTLDGNLISGTFNKKESKEVWNLMRDIEERHAYS
HHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCEEECCCCCHHHHHHHHHHHHHHHHHHHH
ESYIKFFKKDEVFIIKYKIVSEYSSPILRAYLPKPETLGMIIFSIIFFIEIVILSKVFGK
HHHHHHHCCCCEEEEEEEEHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
KFNIEMELLKNTTEKIEQQDLDFVVESSKIYEINNVLFSMDKMKLALKDSLEKQWMLEEN
CCCCHHHHHHHHHHHHHHHCHHHEECCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RKEQISALAHDIKTPLTIIHGNTDLLIEINENPELLEYMEYISKGVTQIEKYINTLIDIS
HHHHHHHHHHHHCCCEEEEECCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
KTENGYILNKEAISMSEFIKDVLVQIEALASTKNLDVEFYKQDNLPKNITIDRELLFRAI
CCCCCEEECHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCEECHHHHHHHH
MNIISNAIDYSPSQSKLYISVSVCNKYLKFVVTDCGSGFSKADLSKATEQFYTGDLSRNS
HHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCC
KSHYGMGLYIVNNIVQKHNGILYIENSVKTGGAMVTIEIPIV
CCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8439156; 8085823 [H]