Click here to switch to the map view.

The map label for this gene is tepA [H]

Identifier: 126698922

GI number: 126698922

Start: 1533436

End: 1534170

Strand: Direct

Name: tepA [H]

Synonym: CD1323

Alternate gene names: 126698922

Gene position: 1533436-1534170 (Clockwise)

Preceding gene: 126698921

Following gene: 126698923

Centisome position: 35.74

GC content: 31.16

Gene sequence:

>735_bases
ATGAAAGATTATAGAGAAGATAATGAAAGATTTGATAAAGATAATTGTAGTGAAGAATCTAGTTGCGAAGAGAGAGAATG
TATAAAGCAATTTGGTACCAATGAAATGCCACCTCAACCTCCTAAAGATATTCAATGTATAACTATAATTGGAGAAATTG
AGGGACATTTTATAGGAAATCCTCAAAAAAAAGCTACAAAGTACGAGCATATAATTCCAATGCTATACTCAATTGAGGAA
AGTAATGATGTTAAAGGAGTGTTAGTTGTTTTAAACACTGTTGGAGGAGATATTGAAGCAGGTTTAGCTATTGCAGAACT
TTTAAACAGTACATCTAAAAAAGTAGTTACTCTTGTTCTAGGAGGAAGTCATAGTATAGGTGTTCCTCTTGCTACAGCAG
GAGATTACTCATTTATAGCACCAACAGCAACCATGATTATACATCCAGTGCGAACTACTGGTCTTGTGATTGGTATAAAT
GAGACTTTTGAGTATTTTAAAAAGATGCAAGATAGAATAATTCAATTTATAATAAGAACATCAAATATCAAAAAAGATGT
ACTTGAAAAACTTATGCATGAAAAAGATGAACTTGTAAGTGATGTTGGTAGTGTATTAATAGGAAAAGAAGCTGTGGATT
ATGGTCTTATAGATGAAGTTGGAGGACTAAAAGAAGCACTTAAAAAATTAAGAGAGCTTATAAAAGAATCTGAGGAAGAA
AAAAATAATGATTAA

Upstream 100 bases:

>100_bases
AATTATTTTTTAATCAAATTTAAGAATTTCATATATATCTTAATATATTTGATTTATTTGGTAAAACTAAATTAAATATA
ATTTTGATAGGAGTTTTTTT

Downstream 100 bases:

>100_bases
AATTTGTTTAAATAATAAAATTTATGATATAATGATGTAATAAAGTAGGAGTGTTAGTGTTAAGGCATTAGCACTTTTTG
CTGTGAAGGGGTAAAACTTG

Product: translocation-enhancing protein TepA

Products: Small peptides [C]

Alternate protein names: NA

Number of amino acids: Translated: 244; Mature: 244

Protein sequence:

>244_residues
MKDYREDNERFDKDNCSEESSCEERECIKQFGTNEMPPQPPKDIQCITIIGEIEGHFIGNPQKKATKYEHIIPMLYSIEE
SNDVKGVLVVLNTVGGDIEAGLAIAELLNSTSKKVVTLVLGGSHSIGVPLATAGDYSFIAPTATMIIHPVRTTGLVIGIN
ETFEYFKKMQDRIIQFIIRTSNIKKDVLEKLMHEKDELVSDVGSVLIGKEAVDYGLIDEVGGLKEALKKLRELIKESEEE
KNND

Sequences:

>Translated_244_residues
MKDYREDNERFDKDNCSEESSCEERECIKQFGTNEMPPQPPKDIQCITIIGEIEGHFIGNPQKKATKYEHIIPMLYSIEE
SNDVKGVLVVLNTVGGDIEAGLAIAELLNSTSKKVVTLVLGGSHSIGVPLATAGDYSFIAPTATMIIHPVRTTGLVIGIN
ETFEYFKKMQDRIIQFIIRTSNIKKDVLEKLMHEKDELVSDVGSVLIGKEAVDYGLIDEVGGLKEALKKLRELIKESEEE
KNND
>Mature_244_residues
MKDYREDNERFDKDNCSEESSCEERECIKQFGTNEMPPQPPKDIQCITIIGEIEGHFIGNPQKKATKYEHIIPMLYSIEE
SNDVKGVLVVLNTVGGDIEAGLAIAELLNSTSKKVVTLVLGGSHSIGVPLATAGDYSFIAPTATMIIHPVRTTGLVIGIN
ETFEYFKKMQDRIIQFIIRTSNIKKDVLEKLMHEKDELVSDVGSVLIGKEAVDYGLIDEVGGLKEALKKLRELIKESEEE
KNND

Specific function: This activity is required for efficient translocation of pre-proteins across the membrane [H]

COG id: COG0740

COG function: function code OU; Protease subunit of ATP-dependent Clp proteases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S14 family. TepA subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001907 [H]

Pfam domain/function: PF00574 CLP_protease [H]

EC number: 3.4.21.92 [C]

Molecular weight: Translated: 27171; Mature: 27171

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDYREDNERFDKDNCSEESSCEERECIKQFGTNEMPPQPPKDIQCITIIGEIEGHFIGN
CCCCCCHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCCC
PQKKATKYEHIIPMLYSIEESNDVKGVLVVLNTVGGDIEAGLAIAELLNSTSKKVVTLVL
CHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCCCEEEEEEE
GGSHSIGVPLATAGDYSFIAPTATMIIHPVRTTGLVIGINETFEYFKKMQDRIIQFIIRT
CCCCCCCCEEECCCCCCHHCCHHHHHEEEHHHCEEEEEEHHHHHHHHHHHHHHHHHHHHH
SNIKKDVLEKLMHEKDELVSDVGSVLIGKEAVDYGLIDEVGGLKEALKKLRELIKESEEE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
KNND
CCCC
>Mature Secondary Structure
MKDYREDNERFDKDNCSEESSCEERECIKQFGTNEMPPQPPKDIQCITIIGEIEGHFIGN
CCCCCCHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCCC
PQKKATKYEHIIPMLYSIEESNDVKGVLVVLNTVGGDIEAGLAIAELLNSTSKKVVTLVL
CHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCCCEEEEEEE
GGSHSIGVPLATAGDYSFIAPTATMIIHPVRTTGLVIGINETFEYFKKMQDRIIQFIIRT
CCCCCCCCEEECCCCCCHHCCHHHHHEEEHHHCEEEEEEHHHHHHHHHHHHHHHHHHHHH
SNIKKDVLEKLMHEKDELVSDVGSVLIGKEAVDYGLIDEVGGLKEALKKLRELIKESEEE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
KNND
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: ATP imidodiphosphate; dATP degradation, [C]

Metal ions: Ca2+; Mg2+; Mn2+; Zn2+ [C]

Kcat value (1/min): 800 [C]

Specific activity: NA

Km value (mM): 1.3 {N-succinyl-Leu-Tyr} 0.21 {ATP}} [C]

Substrates: Protein; H2O [C]

Specific reaction: Protein + H2O = small peptides [C]

General reaction: Hydrolysis of peptide bonds [C]

Inhibitor: Diisopropyl fluorophosphate ClpAP; Fluorosulfonyl benzoyl adenosine; Kappa-Casein; Mg2+; NEM hydrolysis; Neohydrin; Succinyl -Leu-Tyr4-methyl coumarin7-amide; Xaa-Tyr-Leu-Tyr-Trp [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]