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The map label for this gene is rnjA [H]

Identifier: 126698886

GI number: 126698886

Start: 1496690

End: 1498366

Strand: Direct

Name: rnjA [H]

Synonym: CD1289

Alternate gene names: 126698886

Gene position: 1496690-1498366 (Clockwise)

Preceding gene: 126698884

Following gene: 126698887

Centisome position: 34.89

GC content: 31.54

Gene sequence:

>1677_bases
ATGCAATTGTTCAAAAAGAACACCAACAAGATAAAGGTAATGGCCTTGGGTGGACTTAACGAAGTTGGAAAAAATATGAC
TGTTGTTGAGTATAAAGATGAAATAATTGTTATAGATGCTGGACTTAGTTTTCCAGAGGATGAAATGTTAGGAGTAGATA
TAGTTATACCAGATATAACTTATTTAGTAAAAAATAGGGATAAAATAAAGGGTATATTTATTACACATGGACATGAAGAC
CATATAGGAGCTCTTCCATATATATTAAAGAAAATAAATGTACCTGTATATGGGGCAAGACTTAGTATAGGTCTTATACA
AGTAAAACTTAAAGAACATAAAATGAATAATGTTAAGTTAAATGTTATAGGTCCTAGACAAGTTATAAAGCTTGATAATA
TGGAAGTAGAGTTTTTGAAAAATAACCATAGTATACCAGATGCTTACTCTATTGCAATACACACAGACCAAGGTATAATA
TACCACACAGGAGATTTTAAGATTGACTTGACTCCTATTGATGGAGATGTTATGGATATGCATAGAATCTGTGAGTTGAG
CAAAAAAGGAGTTCTTTTAATGCTGGCAGATAGTACTAATGCAGAAAAACCAGGATTTACTATGTCTGAAAAGACTGTTG
GGGTAGGTCTTGACGAATTATTTGCAAAAGGAAATGGCAGAAGAATTATAGTTGCTACCTTTGCATCCAACATACACAGA
TTACAACAAATAATAAATACTGCTGAAAAGTTTAATAGAAAAGTTGCTATATCAGGTCGCTCAATGGTCAATGTAGTTGG
AGTTGCAAAAGAATTAGGATACCTAGATATTTCTGATGACATGCTTATAGATTTAAATGATATATGTAAGTATGAAGATA
GTGAATTAGTGATAATAACAACTGGTTCACAAGGTGAACCTATGTCAGCTCTTGCTAGAATGGCTTTTTCAGAGCACAAA
AAAGTAGAAATAAAGAGTGGCGATTTAGTTATAATATCTGCTCATCCAATACCAGGAAATGAAAAATTAATATCAAGAGT
AATCAATTTCTTATTTGAAAAAGGAGCAGAAGTAGTATACAGTGATATAGCTGATATACACGTTTCTGGACATGCCTGTC
AAGAAGAATTAAAACTTATACATGCATTAGTTAGACCAAAGTTTTTTATGCCAGCTCATGGTGAATATAGAATGTTAAAA
AGACATGCTGAAATAGCTGAACAGCTTGGTATGGATAAAGAAAATATTTTTGTCATGCAAACAGGAGATGTTTTAGAATT
AGATAAAAATTCTGCTAAGGTAGCAAACCGTATACAAACAGGAAACATATTAGTTGATGGTCTAGGAGTAGGCGATGTAG
GTAATATAGTTTTAAGGGATAGAAAGCATTTATCAGAAGATGGTCTTATGATAGTTGTAGTTACTATTTCCAAGGATGAG
GGTAAGGTCTTAGCAGGTCCAGATATAATTTCCAGAGGATTCGTATATGTAAGAGAGTCAGAAGATTTAATGGATGGAGC
AAAGGATATAATTAAGAATGTTCTAAATGAATGTGAAGAGAAAAATATTAAAGAATGGGCTTATTTAAAGAATAATATTA
AAGAAAACCTAAAAGAATATTTATATCAAAAAACTAAACGAAATCCAATGATACTTCCTATCATAATGGAAGTATAA

Upstream 100 bases:

>100_bases
CGAATTATGGTACAATAAGAATAACTGGTAGCGAGTGCGTGATTTGATATTATTTATGATTGTAGAACGTGTTAAAAAAT
TAAATAGACAGAAGGAGATG

Downstream 100 bases:

>100_bases
TATAAAAAAACTATAAATTTGAAAATTAGACCTCTTGTAATTTTTAAAGTAATATCAAAAATCTAATTGATATTATCTTA
AGAATTACAAGAGGTCTTTT

Product: metallo beta-lactamase

Products: NA

Alternate protein names: RNase J1 [H]

Number of amino acids: Translated: 558; Mature: 558

Protein sequence:

>558_residues
MQLFKKNTNKIKVMALGGLNEVGKNMTVVEYKDEIIVIDAGLSFPEDEMLGVDIVIPDITYLVKNRDKIKGIFITHGHED
HIGALPYILKKINVPVYGARLSIGLIQVKLKEHKMNNVKLNVIGPRQVIKLDNMEVEFLKNNHSIPDAYSIAIHTDQGII
YHTGDFKIDLTPIDGDVMDMHRICELSKKGVLLMLADSTNAEKPGFTMSEKTVGVGLDELFAKGNGRRIIVATFASNIHR
LQQIINTAEKFNRKVAISGRSMVNVVGVAKELGYLDISDDMLIDLNDICKYEDSELVIITTGSQGEPMSALARMAFSEHK
KVEIKSGDLVIISAHPIPGNEKLISRVINFLFEKGAEVVYSDIADIHVSGHACQEELKLIHALVRPKFFMPAHGEYRMLK
RHAEIAEQLGMDKENIFVMQTGDVLELDKNSAKVANRIQTGNILVDGLGVGDVGNIVLRDRKHLSEDGLMIVVVTISKDE
GKVLAGPDIISRGFVYVRESEDLMDGAKDIIKNVLNECEEKNIKEWAYLKNNIKENLKEYLYQKTKRNPMILPIIMEV

Sequences:

>Translated_558_residues
MQLFKKNTNKIKVMALGGLNEVGKNMTVVEYKDEIIVIDAGLSFPEDEMLGVDIVIPDITYLVKNRDKIKGIFITHGHED
HIGALPYILKKINVPVYGARLSIGLIQVKLKEHKMNNVKLNVIGPRQVIKLDNMEVEFLKNNHSIPDAYSIAIHTDQGII
YHTGDFKIDLTPIDGDVMDMHRICELSKKGVLLMLADSTNAEKPGFTMSEKTVGVGLDELFAKGNGRRIIVATFASNIHR
LQQIINTAEKFNRKVAISGRSMVNVVGVAKELGYLDISDDMLIDLNDICKYEDSELVIITTGSQGEPMSALARMAFSEHK
KVEIKSGDLVIISAHPIPGNEKLISRVINFLFEKGAEVVYSDIADIHVSGHACQEELKLIHALVRPKFFMPAHGEYRMLK
RHAEIAEQLGMDKENIFVMQTGDVLELDKNSAKVANRIQTGNILVDGLGVGDVGNIVLRDRKHLSEDGLMIVVVTISKDE
GKVLAGPDIISRGFVYVRESEDLMDGAKDIIKNVLNECEEKNIKEWAYLKNNIKENLKEYLYQKTKRNPMILPIIMEV
>Mature_558_residues
MQLFKKNTNKIKVMALGGLNEVGKNMTVVEYKDEIIVIDAGLSFPEDEMLGVDIVIPDITYLVKNRDKIKGIFITHGHED
HIGALPYILKKINVPVYGARLSIGLIQVKLKEHKMNNVKLNVIGPRQVIKLDNMEVEFLKNNHSIPDAYSIAIHTDQGII
YHTGDFKIDLTPIDGDVMDMHRICELSKKGVLLMLADSTNAEKPGFTMSEKTVGVGLDELFAKGNGRRIIVATFASNIHR
LQQIINTAEKFNRKVAISGRSMVNVVGVAKELGYLDISDDMLIDLNDICKYEDSELVIITTGSQGEPMSALARMAFSEHK
KVEIKSGDLVIISAHPIPGNEKLISRVINFLFEKGAEVVYSDIADIHVSGHACQEELKLIHALVRPKFFMPAHGEYRMLK
RHAEIAEQLGMDKENIFVMQTGDVLELDKNSAKVANRIQTGNILVDGLGVGDVGNIVLRDRKHLSEDGLMIVVVTISKDE
GKVLAGPDIISRGFVYVRESEDLMDGAKDIIKNVLNECEEKNIKEWAYLKNNIKENLKEYLYQKTKRNPMILPIIMEV

Specific function: Cleaves the 5'-leader sequence of certain mRNAs. May play a role in maturation and stability of specific mRNAs. Is also required for the maturation of the 16S rRNA. Acts preferentially on 16S rRNA precursors after association of the 30S and 50S ribosomal

COG id: COG0595

COG function: function code R; Predicted hydrolase of the metallo-beta-lactamase superfamily

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. RNase J subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR011108
- InterPro:   IPR004613
- InterPro:   IPR001587 [H]

Pfam domain/function: PF00753 Lactamase_B; PF07521 RMMBL [H]

EC number: NA

Molecular weight: Translated: 62376; Mature: 62376

Theoretical pI: Translated: 6.47; Mature: 6.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLFKKNTNKIKVMALGGLNEVGKNMTVVEYKDEIIVIDAGLSFPEDEMLGVDIVIPDIT
CCCCCCCCCEEEEEEECCHHHHCCCEEEEEECCCEEEEECCCCCCCCCCEEEEEEECCCE
YLVKNRDKIKGIFITHGHEDHIGALPYILKKINVPVYGARLSIGLIQVKLKEHKMNNVKL
EECCCCCCEEEEEEECCCCCCCCHHHHHHHHCCCCEEEEEEEEEEEEEEEECCCCCCEEE
NVIGPRQVIKLDNMEVEFLKNNHSIPDAYSIAIHTDQGIIYHTGDFKIDLTPIDGDVMDM
EEECCHHEEEECCCEEEEEECCCCCCCEEEEEEECCCCEEEECCCEEEEEEECCCCHHHH
HRICELSKKGVLLMLADSTNAEKPGFTMSEKTVGVGLDELFAKGNGRRIIVATFASNIHR
HHHHHHCCCCEEEEEECCCCCCCCCCEECCCEECCCHHHHHHCCCCCEEEEEHHHHHHHH
LQQIINTAEKFNRKVAISGRSMVNVVGVAKELGYLDISDDMLIDLNDICKYEDSELVIIT
HHHHHHHHHHHCCEEEECCCCHHHHHHHHHHCCEEECCCCEEEEHHHHCCCCCCCEEEEE
TGSQGEPMSALARMAFSEHKKVEIKSGDLVIISAHPIPGNEKLISRVINFLFEKGAEVVY
ECCCCCCHHHHHHHHHCCCCEEEEECCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEE
SDIADIHVSGHACQEELKLIHALVRPKFFMPAHGEYRMLKRHAEIAEQLGMDKENIFVMQ
CCHHEEEECCHHHHHHHHHHHHHHCCCEECCCCCHHHHHHHHHHHHHHHCCCCCCEEEEE
TGDVLELDKNSAKVANRIQTGNILVDGLGVGDVGNIVLRDRKHLSEDGLMIVVVTISKDE
CCCEEEECCCCHHHHHHEECCCEEEEECCCCCCCCEEEECCCCCCCCCEEEEEEEEECCC
GKVLAGPDIISRGFVYVRESEDLMDGAKDIIKNVLNECEEKNIKEWAYLKNNIKENLKEY
CCEEECCCHHHCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
LYQKTKRNPMILPIIMEV
HHHHHCCCCEEEEEEEEC
>Mature Secondary Structure
MQLFKKNTNKIKVMALGGLNEVGKNMTVVEYKDEIIVIDAGLSFPEDEMLGVDIVIPDIT
CCCCCCCCCEEEEEEECCHHHHCCCEEEEEECCCEEEEECCCCCCCCCCEEEEEEECCCE
YLVKNRDKIKGIFITHGHEDHIGALPYILKKINVPVYGARLSIGLIQVKLKEHKMNNVKL
EECCCCCCEEEEEEECCCCCCCCHHHHHHHHCCCCEEEEEEEEEEEEEEEECCCCCCEEE
NVIGPRQVIKLDNMEVEFLKNNHSIPDAYSIAIHTDQGIIYHTGDFKIDLTPIDGDVMDM
EEECCHHEEEECCCEEEEEECCCCCCCEEEEEEECCCCEEEECCCEEEEEEECCCCHHHH
HRICELSKKGVLLMLADSTNAEKPGFTMSEKTVGVGLDELFAKGNGRRIIVATFASNIHR
HHHHHHCCCCEEEEEECCCCCCCCCCEECCCEECCCHHHHHHCCCCCEEEEEHHHHHHHH
LQQIINTAEKFNRKVAISGRSMVNVVGVAKELGYLDISDDMLIDLNDICKYEDSELVIIT
HHHHHHHHHHHCCEEEECCCCHHHHHHHHHHCCEEECCCCEEEEHHHHCCCCCCCEEEEE
TGSQGEPMSALARMAFSEHKKVEIKSGDLVIISAHPIPGNEKLISRVINFLFEKGAEVVY
ECCCCCCHHHHHHHHHCCCCEEEEECCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEE
SDIADIHVSGHACQEELKLIHALVRPKFFMPAHGEYRMLKRHAEIAEQLGMDKENIFVMQ
CCHHEEEECCHHHHHHHHHHHHHHCCCEECCCCCHHHHHHHHHHHHHHHCCCCCCEEEEE
TGDVLELDKNSAKVANRIQTGNILVDGLGVGDVGNIVLRDRKHLSEDGLMIVVVTISKDE
CCCEEEECCCCHHHHHHEECCCEEEEECCCCCCCCEEEECCCCCCCCCEEEEEEEEECCC
GKVLAGPDIISRGFVYVRESEDLMDGAKDIIKNVLNECEEKNIKEWAYLKNNIKENLKEY
CCEEECCCHHHCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
LYQKTKRNPMILPIIMEV
HHHHHCCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969500; 9384377 [H]