Click here to switch to the map view.

The map label for this gene is topA [H]

Identifier: 126698871

GI number: 126698871

Start: 1479494

End: 1481581

Strand: Direct

Name: topA [H]

Synonym: CD1274

Alternate gene names: 126698871

Gene position: 1479494-1481581 (Clockwise)

Preceding gene: 126698870

Following gene: 126698872

Centisome position: 34.49

GC content: 30.65

Gene sequence:

>2088_bases
ATGGCCAAAACATTAGTCATCGTAGAATCGCCTGCAAAAGCTAAAACTATAGAAAAATTTTTAGGGAAAAGTCATTACAC
AGTTAAAGCATCAGTTGGTCATGTCAGAGATTTACCTAAAAGTAAATTAGGTGTAGATATAGAAAATAATTTTGAACCTC
AATATATAAATATAAGAGGAAAAGGCGATGTAATAAAAGAATTAAAAAAAGAAGCTAAAAAATCTAAACAAGTATATCTA
GCAACTGACCCTGATAGAGAAGGTGAAGCTATATCATGGCATTTAGCTCATATACTAAATTTAGATGAATCTGAGGATTG
TAGAATAGAGTTTAATGAGATAACTAAGGATGCAATTAAAAAAGCAATTAAACATCCTAGAAATATTGATATAAATTTGG
TTGATGCACAACAGGCAAGAAGAGTGCTAGACAGACTTTTAGGTTATCAAATAAGTCCTATATTATGGCAAAAGGTTAGA
AAAGGTCTTAGTGCAGGTAGAGTGCAATCTGTTACAACAAAGTTAATTTGTGACAGAGAAAAAGAAATAAAGGCATTTAT
TCCAAAAGAATATTGGACAATAGATGTTGAGGCTAAGACAGTAAAAGACAAAGATATAACTTTGAAATTTTATGGTAAAA
ATGATGAAAAAATTGAATTAGAAAATGAAGAAGCTGTAAATGAAATACTAAAAGAAATGGAAGGTAAAGATTTAAAGGTT
GAGAAGATAGAATCTAAATCAAGACGTAAATCAGCTCCAAAACCATTTACAACAAGTATGCTTCAACAAGAAGGAGCAAA
TAAGTTATTTTTTACTACTAAAAAAACAATGATGATAGCACAAGAATTATATGAAGGTATCGACATAGAAGGTGAAGGTA
CTGTTGGTCTTATATCATATATAAGAACTGACTCTAAAAGAATATCTGATGAAGCAAAAGAAAAAGCTAAGGGCTATATC
GTAAACAATTTAGGAGAAAACTACTATAAAAACACTGGTGATAAAAAAGAAAAAAAGGATACTAAAAAGGTTCAAGATGC
CCATGAGGCAATAAGACCAACCTCAGTTGATAGAACTCCTGAGAGTATAAAAGCCTCATTGAGCAAAGACCAGTACAAGT
TATACAATTTAATTTGGAGAAGGTTTGTAGCTAGTCAAATGGAAGATTCAGTGTTTGATATACTAAATGTAGAATGTAAA
ATAGGCGATATTGTATTTAAGGCAACTGGTTCAAAAATGAAATTTGATGGTTATACCAAGGTATATAATTTTTCAGAAAG
AGAAGACAAAATTTTACCAAGTATAGAAAAAGGAGATATATTAAATATAGAAAATATATTGCCAGACCAACATTTTACAC
AACCACCAGCAAGATATACAGAAGCTAGTTTAGTAAAAACACTAGAAGAACTAGGTATAGGTAGACCAAGTACGTATGCT
CCAACAATAGCTACTATACTGAATAGAGAGTATGTTGAAAAGCAAGGAACAAGTCTATGTCCAACAGAACTTGGAATAAT
AGTTACTGATATTTTGGAAAATAATTTCCATAAATTTATAGATGTAGATTTTACTGCTGATATGGAAAGTAAACTTGATG
TTATAGAAGAAGGCAATACAGATTGGAAAAAGATTGTATCAGAATCATATGCACCACTTAAAGAGGCTATTGAAGAAGCT
CTTGAAAATATAGAAAAAGTAAATATGGATGAAGAAACTGATGAGATTTGCGAGAACTGTGGTTCTAATATGGTTATCAA
ATATGGTAGATTTGGAAAGTTTATGGCTTGTAAAAACTATCCCGATTGTAAAACAACTAGACCAATTATGAATAAAATTG
GTGTTAAATGTCCAAAATGTGAAGAAGGAGATATTATACTTAGAAAATCTAAAAGAGGTAAAGCTTTTTATGGGTGTTCT
AATTATCCAAACTGTGACTTTGTAGCTTGGAATAAGCCAACAGGAGAGTTTTGTGAGAAGTGTGGTTCATATATGGTTGA
AAAAGTGACTAAATCTGAAACAAAAACAGTGTGTTCTAATAAAGAATGTAATAAAGAGACAAAAGAGCATAATGACGGAA
AAGAGTGA

Upstream 100 bases:

>100_bases
TCAATGAATTAGTACTAAATGAGTTAGTAGTAGAAATGAATAATAAAACGTACAGTTTAAATGTATAAATTAAGATTAGG
TAGTGGGGGTGTATGGTTTT

Downstream 100 bases:

>100_bases
AAATTGAAAAAAATCACATAATTCTCTTGAAAAAGGTTCCAATCTATGATAATCTTTCAAGTGTAAACAACTAATAGAAA
AATAAATTTTCATTAAATGT

Product: DNA topoisomerase I

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 695; Mature: 694

Protein sequence:

>695_residues
MAKTLVIVESPAKAKTIEKFLGKSHYTVKASVGHVRDLPKSKLGVDIENNFEPQYINIRGKGDVIKELKKEAKKSKQVYL
ATDPDREGEAISWHLAHILNLDESEDCRIEFNEITKDAIKKAIKHPRNIDINLVDAQQARRVLDRLLGYQISPILWQKVR
KGLSAGRVQSVTTKLICDREKEIKAFIPKEYWTIDVEAKTVKDKDITLKFYGKNDEKIELENEEAVNEILKEMEGKDLKV
EKIESKSRRKSAPKPFTTSMLQQEGANKLFFTTKKTMMIAQELYEGIDIEGEGTVGLISYIRTDSKRISDEAKEKAKGYI
VNNLGENYYKNTGDKKEKKDTKKVQDAHEAIRPTSVDRTPESIKASLSKDQYKLYNLIWRRFVASQMEDSVFDILNVECK
IGDIVFKATGSKMKFDGYTKVYNFSEREDKILPSIEKGDILNIENILPDQHFTQPPARYTEASLVKTLEELGIGRPSTYA
PTIATILNREYVEKQGTSLCPTELGIIVTDILENNFHKFIDVDFTADMESKLDVIEEGNTDWKKIVSESYAPLKEAIEEA
LENIEKVNMDEETDEICENCGSNMVIKYGRFGKFMACKNYPDCKTTRPIMNKIGVKCPKCEEGDIILRKSKRGKAFYGCS
NYPNCDFVAWNKPTGEFCEKCGSYMVEKVTKSETKTVCSNKECNKETKEHNDGKE

Sequences:

>Translated_695_residues
MAKTLVIVESPAKAKTIEKFLGKSHYTVKASVGHVRDLPKSKLGVDIENNFEPQYINIRGKGDVIKELKKEAKKSKQVYL
ATDPDREGEAISWHLAHILNLDESEDCRIEFNEITKDAIKKAIKHPRNIDINLVDAQQARRVLDRLLGYQISPILWQKVR
KGLSAGRVQSVTTKLICDREKEIKAFIPKEYWTIDVEAKTVKDKDITLKFYGKNDEKIELENEEAVNEILKEMEGKDLKV
EKIESKSRRKSAPKPFTTSMLQQEGANKLFFTTKKTMMIAQELYEGIDIEGEGTVGLISYIRTDSKRISDEAKEKAKGYI
VNNLGENYYKNTGDKKEKKDTKKVQDAHEAIRPTSVDRTPESIKASLSKDQYKLYNLIWRRFVASQMEDSVFDILNVECK
IGDIVFKATGSKMKFDGYTKVYNFSEREDKILPSIEKGDILNIENILPDQHFTQPPARYTEASLVKTLEELGIGRPSTYA
PTIATILNREYVEKQGTSLCPTELGIIVTDILENNFHKFIDVDFTADMESKLDVIEEGNTDWKKIVSESYAPLKEAIEEA
LENIEKVNMDEETDEICENCGSNMVIKYGRFGKFMACKNYPDCKTTRPIMNKIGVKCPKCEEGDIILRKSKRGKAFYGCS
NYPNCDFVAWNKPTGEFCEKCGSYMVEKVTKSETKTVCSNKECNKETKEHNDGKE
>Mature_694_residues
AKTLVIVESPAKAKTIEKFLGKSHYTVKASVGHVRDLPKSKLGVDIENNFEPQYINIRGKGDVIKELKKEAKKSKQVYLA
TDPDREGEAISWHLAHILNLDESEDCRIEFNEITKDAIKKAIKHPRNIDINLVDAQQARRVLDRLLGYQISPILWQKVRK
GLSAGRVQSVTTKLICDREKEIKAFIPKEYWTIDVEAKTVKDKDITLKFYGKNDEKIELENEEAVNEILKEMEGKDLKVE
KIESKSRRKSAPKPFTTSMLQQEGANKLFFTTKKTMMIAQELYEGIDIEGEGTVGLISYIRTDSKRISDEAKEKAKGYIV
NNLGENYYKNTGDKKEKKDTKKVQDAHEAIRPTSVDRTPESIKASLSKDQYKLYNLIWRRFVASQMEDSVFDILNVECKI
GDIVFKATGSKMKFDGYTKVYNFSEREDKILPSIEKGDILNIENILPDQHFTQPPARYTEASLVKTLEELGIGRPSTYAP
TIATILNREYVEKQGTSLCPTELGIIVTDILENNFHKFIDVDFTADMESKLDVIEEGNTDWKKIVSESYAPLKEAIEEAL
ENIEKVNMDEETDEICENCGSNMVIKYGRFGKFMACKNYPDCKTTRPIMNKIGVKCPKCEEGDIILRKSKRGKAFYGCSN
YPNCDFVAWNKPTGEFCEKCGSYMVEKVTKSETKTVCSNKECNKETKEHNDGKE

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=627, Percent_Identity=25.8373205741627, Blast_Score=156, Evalue=7e-38,
Organism=Homo sapiens, GI4507635, Length=651, Percent_Identity=23.6559139784946, Blast_Score=136, Evalue=7e-32,
Organism=Escherichia coli, GI1787529, Length=763, Percent_Identity=39.4495412844037, Blast_Score=524, Evalue=1e-150,
Organism=Escherichia coli, GI1788061, Length=625, Percent_Identity=24.48, Blast_Score=131, Evalue=1e-31,
Organism=Escherichia coli, GI48994931, Length=114, Percent_Identity=37.719298245614, Blast_Score=103, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI17555378, Length=506, Percent_Identity=25.4940711462451, Blast_Score=144, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI32563869, Length=710, Percent_Identity=25.6338028169014, Blast_Score=124, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6323263, Length=548, Percent_Identity=25, Blast_Score=112, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24585251, Length=630, Percent_Identity=26.3492063492064, Blast_Score=159, Evalue=4e-39,
Organism=Drosophila melanogaster, GI24640096, Length=584, Percent_Identity=24.8287671232877, Blast_Score=132, Evalue=1e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR013498
- InterPro:   IPR005733
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 79244; Mature: 79113

Theoretical pI: Translated: 7.15; Mature: 7.15

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKTLVIVESPAKAKTIEKFLGKSHYTVKASVGHVRDLPKSKLGVDIENNFEPQYINIRG
CCCEEEEEECCCHHHHHHHHHCCCCEEEEECCCHHHCCCHHHCCCCCCCCCCCCEEEEEC
KGDVIKELKKEAKKSKQVYLATDPDREGEAISWHLAHILNLDESEDCRIEFNEITKDAIK
CCHHHHHHHHHHHHCCEEEEEECCCCCCCEEEEEHHHHHCCCCCCCCEEEHHHHHHHHHH
KAIKHPRNIDINLVDAQQARRVLDRLLGYQISPILWQKVRKGLSAGRVQSVTTKLICDRE
HHHCCCCCCEEEEECHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCC
KEIKAFIPKEYWTIDVEAKTVKDKDITLKFYGKNDEKIELENEEAVNEILKEMEGKDLKV
HHHHHCCCCCEEEEEEEEEEECCCCEEEEEECCCCCEEEECCHHHHHHHHHHHCCCCCCH
EKIESKSRRKSAPKPFTTSMLQQEGANKLFFTTKKTMMIAQELYEGIDIEGEGTVGLISY
HHHHHHHHHCCCCCCHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHCCCCCCCCCHHHHHH
IRTDSKRISDEAKEKAKGYIVNNLGENYYKNTGDKKEKKDTKKVQDAHEAIRPTSVDRTP
HHCCHHHHHHHHHHHHCCEEEHHCCCHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCH
ESIKASLSKDQYKLYNLIWRRFVASQMEDSVFDILNVECKIGDIVFKATGSKMKFDGYTK
HHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCEEEEECCCEEEECCCHH
VYNFSEREDKILPSIEKGDILNIENILPDQHFTQPPARYTEASLVKTLEELGIGRPSTYA
EECCCCHHHCCCCCCCCCCEECHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCC
PTIATILNREYVEKQGTSLCPTELGIIVTDILENNFHKFIDVDFTADMESKLDVIEEGNT
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHCCCC
DWKKIVSESYAPLKEAIEEALENIEKVNMDEETDEICENCGSNMVIKYGRFGKFMACKNY
HHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCEEEEECCCCCEEEECCC
PDCKTTRPIMNKIGVKCPKCEEGDIILRKSKRGKAFYGCSNYPNCDFVAWNKPTGEFCEK
CCCCCCHHHHHHHCCCCCCCCCCCEEEEECCCCCEEECCCCCCCCCEEEECCCCHHHHHH
CGSYMVEKVTKSETKTVCSNKECNKETKEHNDGKE
HHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCC
>Mature Secondary Structure 
AKTLVIVESPAKAKTIEKFLGKSHYTVKASVGHVRDLPKSKLGVDIENNFEPQYINIRG
CCEEEEEECCCHHHHHHHHHCCCCEEEEECCCHHHCCCHHHCCCCCCCCCCCCEEEEEC
KGDVIKELKKEAKKSKQVYLATDPDREGEAISWHLAHILNLDESEDCRIEFNEITKDAIK
CCHHHHHHHHHHHHCCEEEEEECCCCCCCEEEEEHHHHHCCCCCCCCEEEHHHHHHHHHH
KAIKHPRNIDINLVDAQQARRVLDRLLGYQISPILWQKVRKGLSAGRVQSVTTKLICDRE
HHHCCCCCCEEEEECHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCC
KEIKAFIPKEYWTIDVEAKTVKDKDITLKFYGKNDEKIELENEEAVNEILKEMEGKDLKV
HHHHHCCCCCEEEEEEEEEEECCCCEEEEEECCCCCEEEECCHHHHHHHHHHHCCCCCCH
EKIESKSRRKSAPKPFTTSMLQQEGANKLFFTTKKTMMIAQELYEGIDIEGEGTVGLISY
HHHHHHHHHCCCCCCHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHCCCCCCCCCHHHHHH
IRTDSKRISDEAKEKAKGYIVNNLGENYYKNTGDKKEKKDTKKVQDAHEAIRPTSVDRTP
HHCCHHHHHHHHHHHHCCEEEHHCCCHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCH
ESIKASLSKDQYKLYNLIWRRFVASQMEDSVFDILNVECKIGDIVFKATGSKMKFDGYTK
HHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCEEEEECCCEEEECCCHH
VYNFSEREDKILPSIEKGDILNIENILPDQHFTQPPARYTEASLVKTLEELGIGRPSTYA
EECCCCHHHCCCCCCCCCCEECHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCC
PTIATILNREYVEKQGTSLCPTELGIIVTDILENNFHKFIDVDFTADMESKLDVIEEGNT
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHCCCC
DWKKIVSESYAPLKEAIEEALENIEKVNMDEETDEICENCGSNMVIKYGRFGKFMACKNY
HHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCEEEEECCCCCEEEECCC
PDCKTTRPIMNKIGVKCPKCEEGDIILRKSKRGKAFYGCSNYPNCDFVAWNKPTGEFCEK
CCCCCCHHHHHHHCCCCCCCCCCCEEEEECCCCCEEECCCCCCCCCEEEECCCCHHHHHH
CGSYMVEKVTKSETKTVCSNKECNKETKEHNDGKE
HHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]