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The map label for this gene is spo0A [H]

Identifier: 126698810

GI number: 126698810

Start: 1412597

End: 1413421

Strand: Direct

Name: spo0A [H]

Synonym: CD1214

Alternate gene names: 126698810

Gene position: 1412597-1413421 (Clockwise)

Preceding gene: 126698809

Following gene: 126698811

Centisome position: 32.93

GC content: 30.42

Gene sequence:

>825_bases
ATGGGGGGATTTTTAGTGGAAAAAATCAAAATAGTTTTAGCAGATGACAATAAGGATTTTTGTCAGGTATTAAAAGAGTA
TTTGTCTAATGAAGATGATATCGATATATTAGGCATAGCTAAGGATGGAATTGAAGCATTAGACTTAGTAAAAAAGACAC
AACCGGACTTATTAATACTAGATGTAATAATGCCACATCTAGATGGATTAGGTGTAATTGAAAAATTGAATACTATGGAT
ATACCTAAAATGCCAAAAATAATAGTACTATCAGCAGTAGGTCAAGATAAGATAACTCAAAGCGCAATAAATCTAGGAGC
AGATTACTACATAGTAAAGCCATTTGATTTTGTTGTGTTTATAAACAGAATTAGAGAGCTAGTTTCTAATAGAGTTACAC
AAGTTGAGCCAAAACCTAGACCAGTTCAAGAGACTCAAATGACTAGAAGTGATTTTGTTAAGAATGTAGGAAATATAGAG
ACAGAAATCACAAATATAATACATGAAATAGGAGTACCAGCTCATATAAAAGGATATTTATATTTAAGAGAAGCAATAAA
AATGGTTATTGATAATGTTGAGCTTTTAGGTGCAGTAACAAAAGAGTTATATCCAAGTATAGCTAAAAAATTCAATACAA
CACCAAGTAGAGTTGAAAGAGCAATAAGACATGCAATAGAGGTTGCATGGAGTAGAGGAAAAGTTGACACAATAAATCAA
TTATTTGGATATACGGTACACAATACTAAAGGAAAACCAACTAATTCAGAATTTATAGCAATGATTGCTGATAAATTAAG
ACTAGAACATAGTATGGTTAAATAA

Upstream 100 bases:

>100_bases
GAGGAATATAATTTTGGAGTGTCGAATATGCTTTAGAGTAGATAATTAGGAAGCAATTGTGTAAAAAGTTTAGTTTTCTG
TAATAAGAAGATGTTTTTTA

Downstream 100 bases:

>100_bases
ACAAGACATAAAAAGTAAGGCTTTTTAATTAAGGCATTGGCTATAAATGCGTATTACAAGCAGCGAAACGGTATAACCAC
TAGGGTTATACCGTTTCGCT

Product: stage 0 sporulation protein A

Products: protein L-glutamate+ methanol; methanol; Tapglu; methanol; Targlu; Trgglu; Tsrglu; NH3; protein-L-glutamate [C]

Alternate protein names: NA

Number of amino acids: Translated: 274; Mature: 273

Protein sequence:

>274_residues
MGGFLVEKIKIVLADDNKDFCQVLKEYLSNEDDIDILGIAKDGIEALDLVKKTQPDLLILDVIMPHLDGLGVIEKLNTMD
IPKMPKIIVLSAVGQDKITQSAINLGADYYIVKPFDFVVFINRIRELVSNRVTQVEPKPRPVQETQMTRSDFVKNVGNIE
TEITNIIHEIGVPAHIKGYLYLREAIKMVIDNVELLGAVTKELYPSIAKKFNTTPSRVERAIRHAIEVAWSRGKVDTINQ
LFGYTVHNTKGKPTNSEFIAMIADKLRLEHSMVK

Sequences:

>Translated_274_residues
MGGFLVEKIKIVLADDNKDFCQVLKEYLSNEDDIDILGIAKDGIEALDLVKKTQPDLLILDVIMPHLDGLGVIEKLNTMD
IPKMPKIIVLSAVGQDKITQSAINLGADYYIVKPFDFVVFINRIRELVSNRVTQVEPKPRPVQETQMTRSDFVKNVGNIE
TEITNIIHEIGVPAHIKGYLYLREAIKMVIDNVELLGAVTKELYPSIAKKFNTTPSRVERAIRHAIEVAWSRGKVDTINQ
LFGYTVHNTKGKPTNSEFIAMIADKLRLEHSMVK
>Mature_273_residues
GGFLVEKIKIVLADDNKDFCQVLKEYLSNEDDIDILGIAKDGIEALDLVKKTQPDLLILDVIMPHLDGLGVIEKLNTMDI
PKMPKIIVLSAVGQDKITQSAINLGADYYIVKPFDFVVFINRIRELVSNRVTQVEPKPRPVQETQMTRSDFVKNVGNIET
EITNIIHEIGVPAHIKGYLYLREAIKMVIDNVELLGAVTKELYPSIAKKFNTTPSRVERAIRHAIEVAWSRGKVDTINQL
FGYTVHNTKGKPTNSEFIAMIADKLRLEHSMVK

Specific function: May play the central regulatory role in sporulation. It may be an element of the effector pathway responsible for the activation of sporulation genes in response to nutritional stress. Spo0A may act in concert with spo0H (a sigma factor) to control the ex

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788192, Length=110, Percent_Identity=37.2727272727273, Blast_Score=74, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR014879
- InterPro:   IPR012052
- InterPro:   IPR011991
- ProDom:   PD010908 [H]

Pfam domain/function: PF00072 Response_reg; PF08769 Spo0A_C [H]

EC number: 3.1.1.61 [C]

Molecular weight: Translated: 30850; Mature: 30719

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGGFLVEKIKIVLADDNKDFCQVLKEYLSNEDDIDILGIAKDGIEALDLVKKTQPDLLIL
CCCCEEEEEEEEEECCCHHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHCCCCEEEH
DVIMPHLDGLGVIEKLNTMDIPKMPKIIVLSAVGQDKITQSAINLGADYYIVKPFDFVVF
HHHHHCCCCCHHHHHHCCCCCCCCCCEEEEEECCCHHHHHHHHHCCCCEEEECHHHHHHH
INRIRELVSNRVTQVEPKPRPVQETQMTRSDFVKNVGNIETEITNIIHEIGVPAHIKGYL
HHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHH
YLREAIKMVIDNVELLGAVTKELYPSIAKKFNTTPSRVERAIRHAIEVAWSRGKVDTINQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHH
LFGYTVHNTKGKPTNSEFIAMIADKLRLEHSMVK
HHCEEEECCCCCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
GGFLVEKIKIVLADDNKDFCQVLKEYLSNEDDIDILGIAKDGIEALDLVKKTQPDLLIL
CCCEEEEEEEEEECCCHHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHCCCCEEEH
DVIMPHLDGLGVIEKLNTMDIPKMPKIIVLSAVGQDKITQSAINLGADYYIVKPFDFVVF
HHHHHCCCCCHHHHHHCCCCCCCCCCEEEEEECCCHHHHHHHHHCCCCEEEECHHHHHHH
INRIRELVSNRVTQVEPKPRPVQETQMTRSDFVKNVGNIETEITNIIHEIGVPAHIKGYL
HHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHH
YLREAIKMVIDNVELLGAVTKELYPSIAKKFNTTPSRVERAIRHAIEVAWSRGKVDTINQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHH
LFGYTVHNTKGKPTNSEFIAMIADKLRLEHSMVK
HHCEEEECCCCCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Protein L-glutamate Omethyl ester; Tapglu-Me; H2O; Targlu-Me; Trgglu-Me; Tsrglu-Me; Tsrgln; Trggln; Targln; Tapgln; protein-L-glutamine [C]

Specific reaction: Protein L-glutamate Omethyl ester + H2O = protein L-glutamate+ methanol H2O + Tapglu-Me = methanol + Tapglu H2O + Targlu-Me = methanol + Targlu H2O + Trgglu-Me = methanol + Trgglu H2O + Tsrglu-Me = methanol + Tsrglu H2O + Tsrgln = NH3 + Tsrglu H2O + Trggl

General reaction: Hydrolysis of carboxylic ester [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA