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The map label for this gene is leuC2 [H]

Identifier: 126698413

GI number: 126698413

Start: 1009321

End: 1011246

Strand: Direct

Name: leuC2 [H]

Synonym: CD0833

Alternate gene names: 126698413

Gene position: 1009321-1011246 (Clockwise)

Preceding gene: 126698412

Following gene: 126698414

Centisome position: 23.53

GC content: 33.96

Gene sequence:

>1926_bases
ATGGGAGATAATATAGTTTATAAAATAATTAAAAAACATATAGTAGATGGAGAAGCTGTTGCTGGAAGTTCAATTGGTAT
AAAAATAGACCAAACTTTAACACAAGATTCTACTGGAACAATGACATATCTTCAACTAGAAGCTATGGGAATAGATAAAG
TAAAGACTAAAAGAAGTGTGGCATTTGTAGACCATAATATGTTACAACAAGGATTTGAAAATGCAGACGACCATAAGTAT
ATACAAACAGTGGCAGATAAATATGGAGTTTATTTCTCTAAGCCAGGAAATGGTATTTGTCATCAAGTATTTTTAGAAAG
ATTTTCAACACCAGGAGATACACTTTTAGGTTCTGATAGCCACACACCTACTGCTGGAGGTGTGGGCATGATGGCTATTG
GAGCTGGTGGATTAGATGTTGCTTTAGCCATGGCAGGAGGAGCATACTATATAAAAGCACCAAAAGTCTGTAAGGTGAAT
CTAGTTGGAAAATTAAATAATATGGTATCATCAAAAGATATAATATTAGAAGTGTTAAGAAAACAAACTGTAAAAGGTGG
AGTAGGTAAAGTTTATGAATATGGTGGAGAAGGTGTCAAAAGCCTATCTGTTCCACAAAGAGCTACAATAACAAATATGG
GGGCTGAACTTGGAGCAACTACTTCAATCTTCCCAAGTGATGAAAAAACACTAGAATTTTTTAAGAGTCAAGGAAGAGAA
GACGCATGGGTGGAGCTAAAACCAGATGCAGATGCAGTTTATGATGAAGAGATTACTATAAACTTGGATGAATTAAAACC
ATTAGCTGCAAAACCTCATAGCCCAGACAATGTAGATGAAGTTGAAAATATAGGAAAGATTAAAATAGACCAAGTGGCAA
TAGGAAGTTGTACAAATTCTTCTTATGAAGACTTAATGAAAGTAGCACAAATCCTGAAAGGAAATAAAGTACATAAGGAT
GTGAGTTTGGTAATAGCACCAGGTTCAAGACAAGTTATGGAGATGATTGCTAGAAATGGGGCTTTGGCAGATATAATAAG
TGCTGGAGCTAGAATACTAGAAAATTCTTGTGGTCCATGTATTGGTATGGGTCAATCTCCAGGTACTGACTCTGTGTCTT
TAAGAACTTTCAATAGAAATTTCTATGGTAGAAGTGGGACTTTGTCAGCACAAGTATATTTAGTAAGTCCAGAAGTGGCA
GCAGTAAGTGCAATCAAGGGAGTGCTTACAGACCCAAGAGAATTTGATATTAAATTTACAAATTTAGATGTAAATGAATT
TTTGATAGATGATAGCATGATAATTAAACCTGCTGATGTAGGAAGTGATGTAGAAGTAGTTAGAGGACCTAATATAAAAC
CTTTCCCTCTTAATACAGAGCTAAGTCAGTCTATAGGTGGAAAAGTTATATTAAAGACAGAAGATAATATAACTACTGAC
CATATAATGCCATCAAATGCTAAGTTACTTCCTTTTAGAAGTAATATACCGTATCTTGCAAACTATTGTTTCAATACTGT
AGATACAGAATTTCCTCAGAGAGCAAAAGACAATAATGGTGGATTTATAGTTGGTGGAGATAATTATGGTCAAGGTTCAA
GTAGAGAACATGCAGCTTTAGCACCATTATATCTAGGTGTTAAAGGGGTAATAGTTAAGAGCTTTGCAAGAATCCACAAG
GCTAACTTGATAAATAGTGGTATAATTCCAATGGAGTTTTGTGATGAGAAAGATTATGAAAATATATCTCTTTTAGATAA
TCTGGAGATACCAAATATTTTAGACAATCTAGGTAGTGGAATTTTAGAAGTTAAGAATACAACTAAAGGAACTTCTTTTA
AGGTCAAAGTAGAATTGTCAGCAAAAGAAGTTGATGTATTAAAAGCTGGTGGAAAATTAAACTACACTAAAAATCAAGCT
AATTAA

Upstream 100 bases:

>100_bases
TCAATTATATTGTGATATACTAGCACATGAGAAAGGCACAACAGAAGAAGAAGCAGTTAGAGGTTCATATATTTAAGATA
TAATTGTTTAGGAGGAAAAT

Downstream 100 bases:

>100_bases
TATGAAAAGAGGGAATATTGAATGTATAAAGTGACACTTATACCAGGAGATGGAATAGGACCAGAAGTTGCGAAAGCTAT
GAAAAAGGTAGTAGAAGCTA

Product: aconitate hydratase

Products: NA

Alternate protein names: Alpha-IPM isomerase 2; IPMI 2; Isopropylmalate isomerase 2 [H]

Number of amino acids: Translated: 641; Mature: 640

Protein sequence:

>641_residues
MGDNIVYKIIKKHIVDGEAVAGSSIGIKIDQTLTQDSTGTMTYLQLEAMGIDKVKTKRSVAFVDHNMLQQGFENADDHKY
IQTVADKYGVYFSKPGNGICHQVFLERFSTPGDTLLGSDSHTPTAGGVGMMAIGAGGLDVALAMAGGAYYIKAPKVCKVN
LVGKLNNMVSSKDIILEVLRKQTVKGGVGKVYEYGGEGVKSLSVPQRATITNMGAELGATTSIFPSDEKTLEFFKSQGRE
DAWVELKPDADAVYDEEITINLDELKPLAAKPHSPDNVDEVENIGKIKIDQVAIGSCTNSSYEDLMKVAQILKGNKVHKD
VSLVIAPGSRQVMEMIARNGALADIISAGARILENSCGPCIGMGQSPGTDSVSLRTFNRNFYGRSGTLSAQVYLVSPEVA
AVSAIKGVLTDPREFDIKFTNLDVNEFLIDDSMIIKPADVGSDVEVVRGPNIKPFPLNTELSQSIGGKVILKTEDNITTD
HIMPSNAKLLPFRSNIPYLANYCFNTVDTEFPQRAKDNNGGFIVGGDNYGQGSSREHAALAPLYLGVKGVIVKSFARIHK
ANLINSGIIPMEFCDEKDYENISLLDNLEIPNILDNLGSGILEVKNTTKGTSFKVKVELSAKEVDVLKAGGKLNYTKNQA
N

Sequences:

>Translated_641_residues
MGDNIVYKIIKKHIVDGEAVAGSSIGIKIDQTLTQDSTGTMTYLQLEAMGIDKVKTKRSVAFVDHNMLQQGFENADDHKY
IQTVADKYGVYFSKPGNGICHQVFLERFSTPGDTLLGSDSHTPTAGGVGMMAIGAGGLDVALAMAGGAYYIKAPKVCKVN
LVGKLNNMVSSKDIILEVLRKQTVKGGVGKVYEYGGEGVKSLSVPQRATITNMGAELGATTSIFPSDEKTLEFFKSQGRE
DAWVELKPDADAVYDEEITINLDELKPLAAKPHSPDNVDEVENIGKIKIDQVAIGSCTNSSYEDLMKVAQILKGNKVHKD
VSLVIAPGSRQVMEMIARNGALADIISAGARILENSCGPCIGMGQSPGTDSVSLRTFNRNFYGRSGTLSAQVYLVSPEVA
AVSAIKGVLTDPREFDIKFTNLDVNEFLIDDSMIIKPADVGSDVEVVRGPNIKPFPLNTELSQSIGGKVILKTEDNITTD
HIMPSNAKLLPFRSNIPYLANYCFNTVDTEFPQRAKDNNGGFIVGGDNYGQGSSREHAALAPLYLGVKGVIVKSFARIHK
ANLINSGIIPMEFCDEKDYENISLLDNLEIPNILDNLGSGILEVKNTTKGTSFKVKVELSAKEVDVLKAGGKLNYTKNQA
N
>Mature_640_residues
GDNIVYKIIKKHIVDGEAVAGSSIGIKIDQTLTQDSTGTMTYLQLEAMGIDKVKTKRSVAFVDHNMLQQGFENADDHKYI
QTVADKYGVYFSKPGNGICHQVFLERFSTPGDTLLGSDSHTPTAGGVGMMAIGAGGLDVALAMAGGAYYIKAPKVCKVNL
VGKLNNMVSSKDIILEVLRKQTVKGGVGKVYEYGGEGVKSLSVPQRATITNMGAELGATTSIFPSDEKTLEFFKSQGRED
AWVELKPDADAVYDEEITINLDELKPLAAKPHSPDNVDEVENIGKIKIDQVAIGSCTNSSYEDLMKVAQILKGNKVHKDV
SLVIAPGSRQVMEMIARNGALADIISAGARILENSCGPCIGMGQSPGTDSVSLRTFNRNFYGRSGTLSAQVYLVSPEVAA
VSAIKGVLTDPREFDIKFTNLDVNEFLIDDSMIIKPADVGSDVEVVRGPNIKPFPLNTELSQSIGGKVILKTEDNITTDH
IMPSNAKLLPFRSNIPYLANYCFNTVDTEFPQRAKDNNGGFIVGGDNYGQGSSREHAALAPLYLGVKGVIVKSFARIHKA
NLINSGIIPMEFCDEKDYENISLLDNLEIPNILDNLGSGILEVKNTTKGTSFKVKVELSAKEVDVLKAGGKLNYTKNQAN

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG1048

COG function: function code C; Aconitase A

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=692, Percent_Identity=34.5375722543353, Blast_Score=319, Evalue=5e-87,
Organism=Homo sapiens, GI8659555, Length=395, Percent_Identity=27.0886075949367, Blast_Score=127, Evalue=4e-29,
Organism=Homo sapiens, GI41352693, Length=396, Percent_Identity=26.2626262626263, Blast_Score=117, Evalue=2e-26,
Organism=Escherichia coli, GI87081781, Length=728, Percent_Identity=27.8846153846154, Blast_Score=228, Evalue=8e-61,
Organism=Escherichia coli, GI1786259, Length=468, Percent_Identity=30.1282051282051, Blast_Score=155, Evalue=7e-39,
Organism=Escherichia coli, GI1787531, Length=491, Percent_Identity=27.6985743380855, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI2367097, Length=437, Percent_Identity=26.5446224256293, Blast_Score=82, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI25149337, Length=690, Percent_Identity=33.4782608695652, Blast_Score=311, Evalue=9e-85,
Organism=Caenorhabditis elegans, GI32564738, Length=678, Percent_Identity=33.7758112094395, Blast_Score=307, Evalue=9e-84,
Organism=Caenorhabditis elegans, GI25149342, Length=564, Percent_Identity=32.8014184397163, Blast_Score=253, Evalue=2e-67,
Organism=Caenorhabditis elegans, GI17568399, Length=714, Percent_Identity=26.3305322128852, Blast_Score=174, Evalue=1e-43,
Organism=Saccharomyces cerevisiae, GI6322261, Length=740, Percent_Identity=32.8378378378378, Blast_Score=342, Evalue=8e-95,
Organism=Saccharomyces cerevisiae, GI6323335, Length=650, Percent_Identity=34.1538461538462, Blast_Score=331, Evalue=2e-91,
Organism=Saccharomyces cerevisiae, GI6320440, Length=619, Percent_Identity=29.2407108239095, Blast_Score=220, Evalue=5e-58,
Organism=Saccharomyces cerevisiae, GI6321429, Length=675, Percent_Identity=28.1481481481481, Blast_Score=185, Evalue=2e-47,
Organism=Drosophila melanogaster, GI281365315, Length=715, Percent_Identity=33.2867132867133, Blast_Score=319, Evalue=4e-87,
Organism=Drosophila melanogaster, GI17864292, Length=715, Percent_Identity=33.2867132867133, Blast_Score=319, Evalue=4e-87,
Organism=Drosophila melanogaster, GI28571643, Length=696, Percent_Identity=33.3333333333333, Blast_Score=317, Evalue=2e-86,
Organism=Drosophila melanogaster, GI161076999, Length=684, Percent_Identity=33.7719298245614, Blast_Score=314, Evalue=1e-85,
Organism=Drosophila melanogaster, GI17137564, Length=487, Percent_Identity=27.1047227926078, Blast_Score=115, Evalue=7e-26,
Organism=Drosophila melanogaster, GI24645686, Length=503, Percent_Identity=27.037773359841, Blast_Score=113, Evalue=5e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR011826
- InterPro:   IPR015936
- InterPro:   IPR006251 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 69119; Mature: 68987

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGDNIVYKIIKKHIVDGEAVAGSSIGIKIDQTLTQDSTGTMTYLQLEAMGIDKVKTKRSV
CCCHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCCCEEEEEEEECCCCHHHHCCCE
AFVDHNMLQQGFENADDHKYIQTVADKYGVYFSKPGNGICHQVFLERFSTPGDTLLGSDS
EEHHHHHHHHHCCCCCCHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHCCCCCCEECCCC
HTPTAGGVGMMAIGAGGLDVALAMAGGAYYIKAPKVCKVNLVGKLNNMVSSKDIILEVLR
CCCCCCCCEEEEECCCCHHEEEEECCCEEEEECCCEEEEEEHHHHHHHHCHHHHHHHHHH
KQTVKGGVGKVYEYGGEGVKSLSVPQRATITNMGAELGATTSIFPSDEKTLEFFKSQGRE
HHHHCCCCCHHHHCCCCCCEECCCCCCHHHHHCCHHHCCCCEECCCCHHHHHHHHHCCCC
DAWVELKPDADAVYDEEITINLDELKPLAAKPHSPDNVDEVENIGKIKIDQVAIGSCTNS
CCEEEECCCCCCEECCEEEEEHHHCCCCCCCCCCCCCHHHHHCCCCEEEEEEEECCCCCC
SYEDLMKVAQILKGNKVHKDVSLVIAPGSRQVMEMIARNGALADIISAGARILENSCGPC
CHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCE
IGMGQSPGTDSVSLRTFNRNFYGRSGTLSAQVYLVSPEVAAVSAIKGVLTDPREFDIKFT
EECCCCCCCCCEEEEEECCCCCCCCCCEEEEEEEECCCHHHHHHHHHHCCCCCCEEEEEE
NLDVNEFLIDDSMIIKPADVGSDVEVVRGPNIKPFPLNTELSQSIGGKVILKTEDNITTD
ECCHHHEEECCCEEEEECCCCCCCEEEECCCCCCCCCCCHHHHHCCCEEEEEECCCCCCC
HIMPSNAKLLPFRSNIPYLANYCFNTVDTEFPQRAKDNNGGFIVGGDNYGQGSSREHAAL
CCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCHHCCCCCCCEEECCCCCCCCCCCCCHHH
APLYLGVKGVIVKSFARIHKANLINSGIIPMEFCDEKDYENISLLDNLEIPNILDNLGSG
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCEEEEECCCCCHHHHHHCCC
ILEVKNTTKGTSFKVKVELSAKEVDVLKAGGKLNYTKNQAN
EEEEECCCCCCEEEEEEEECCCEEEEEECCCEEECCCCCCC
>Mature Secondary Structure 
GDNIVYKIIKKHIVDGEAVAGSSIGIKIDQTLTQDSTGTMTYLQLEAMGIDKVKTKRSV
CCHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCCCEEEEEEEECCCCHHHHCCCE
AFVDHNMLQQGFENADDHKYIQTVADKYGVYFSKPGNGICHQVFLERFSTPGDTLLGSDS
EEHHHHHHHHHCCCCCCHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHCCCCCCEECCCC
HTPTAGGVGMMAIGAGGLDVALAMAGGAYYIKAPKVCKVNLVGKLNNMVSSKDIILEVLR
CCCCCCCCEEEEECCCCHHEEEEECCCEEEEECCCEEEEEEHHHHHHHHCHHHHHHHHHH
KQTVKGGVGKVYEYGGEGVKSLSVPQRATITNMGAELGATTSIFPSDEKTLEFFKSQGRE
HHHHCCCCCHHHHCCCCCCEECCCCCCHHHHHCCHHHCCCCEECCCCHHHHHHHHHCCCC
DAWVELKPDADAVYDEEITINLDELKPLAAKPHSPDNVDEVENIGKIKIDQVAIGSCTNS
CCEEEECCCCCCEECCEEEEEHHHCCCCCCCCCCCCCHHHHHCCCCEEEEEEEECCCCCC
SYEDLMKVAQILKGNKVHKDVSLVIAPGSRQVMEMIARNGALADIISAGARILENSCGPC
CHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCE
IGMGQSPGTDSVSLRTFNRNFYGRSGTLSAQVYLVSPEVAAVSAIKGVLTDPREFDIKFT
EECCCCCCCCCEEEEEECCCCCCCCCCEEEEEEEECCCHHHHHHHHHHCCCCCCEEEEEE
NLDVNEFLIDDSMIIKPADVGSDVEVVRGPNIKPFPLNTELSQSIGGKVILKTEDNITTD
ECCHHHEEECCCEEEEECCCCCCCEEEECCCCCCCCCCCHHHHHCCCEEEEEECCCCCCC
HIMPSNAKLLPFRSNIPYLANYCFNTVDTEFPQRAKDNNGGFIVGGDNYGQGSSREHAAL
CCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCHHCCCCCCCEEECCCCCCCCCCCCCHHH
APLYLGVKGVIVKSFARIHKANLINSGIIPMEFCDEKDYENISLLDNLEIPNILDNLGSG
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCEEEEECCCCCHHHHHHCCC
ILEVKNTTKGTSFKVKVELSAKEVDVLKAGGKLNYTKNQAN
EEEEECCCCCCEEEEEEEECCCEEEEEECCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]