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The map label for this gene is leuB [H]

Identifier: 126698414

GI number: 126698414

Start: 1011268

End: 1012263

Strand: Direct

Name: leuB [H]

Synonym: CD0834

Alternate gene names: 126698414

Gene position: 1011268-1012263 (Clockwise)

Preceding gene: 126698413

Following gene: 126698415

Centisome position: 23.57

GC content: 32.73

Gene sequence:

>996_bases
ATGTATAAAGTGACACTTATACCAGGAGATGGAATAGGACCAGAAGTTGCGAAAGCTATGAAAAAGGTAGTAGAAGCTAC
TGGAGTAGAAATAGAATGGGAAGAAGTTAATGCAGGAGAGGCAGTTATTGAAGAGTATGGTACTCCACTTCCTGAATATA
TTATAGATAGTATAAAAAAGAACAAAATAGCTATAAAAGGCCCAATTACTACTCCTGTAGGTAAAGGATTTAGGAGTGTA
AATGTAACTTTAAGACAGGCATTAGATTTGTATGTCAATTTAAGACCTATTAAAAGTTTTAAGGGGATAAAATCAAGATA
TGAAGATGTTGATTTAGTGGTTGTAAGAGAAAATACAGAAGATTTATATGCTGGAATAGAACATAAAATAGGTGATTATG
CAGCTGAAAGTATAAAGATAATAACAAGAAGTGCAAGTGAAAGAATCGTTGATTTTGCATGTAACTATGTTAAAGATAAT
AAGAGAAAAAAAGTTACAGCTATACATAAGGCAAATATAATGAAAATGTCTGATGGATTGTTTTTAGATGTGTTTAGAGA
AGTTGCATCAAAACATGGTGTAGAATATGATGATTTGATTGTAGATGCAGCAGCTATGAACTTAGTTTTAAATCCAGAAA
ATTATGATGTAATGGTAATGCCAAATCTTTATGGAGATATACTATCAGATTTAGGTGCTGGGCTTGTTGGAGGTTTAGGT
ATAATTCCAAGTGCAAATATTGGTAAAGATTGTGCGATATTTGAAGCTGTTCATGGAAGTGCTCCACAAATAGCAGGTCA
AAATAAGGCTAATCCAACAGCACTTATTCAATCATCAGTAATGATGCTTAGATACTTAGGTGAATATGAAAATGCACAAA
AAATAGAAACAGCTTTAGAAAAAGTATTTCTTGAAGGAAGTAAATTGACTGTAGATTTGGGTGGCTCAGCTTCTACTACA
GAGTTTGCGGATGAGGTGTGTAAGTATATTGTATAG

Upstream 100 bases:

>100_bases
AGTAGAATTGTCAGCAAAAGAAGTTGATGTATTAAAAGCTGGTGGAAAATTAAACTACACTAAAAATCAAGCTAATTAAT
ATGAAAAGAGGGAATATTGA

Downstream 100 bases:

>100_bases
ATTTTATTTAGGGAAAAAGTAAAATATAGATTGAAGGAAAGAGGCTTTCTCAAAATAGAGATAAGCCTTTTTTATTGGAA
TGATAAAATATATTTGATTT

Product: isocitrate/3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; IMDH; Beta-IPM dehydrogenase [H]

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MYKVTLIPGDGIGPEVAKAMKKVVEATGVEIEWEEVNAGEAVIEEYGTPLPEYIIDSIKKNKIAIKGPITTPVGKGFRSV
NVTLRQALDLYVNLRPIKSFKGIKSRYEDVDLVVVRENTEDLYAGIEHKIGDYAAESIKIITRSASERIVDFACNYVKDN
KRKKVTAIHKANIMKMSDGLFLDVFREVASKHGVEYDDLIVDAAAMNLVLNPENYDVMVMPNLYGDILSDLGAGLVGGLG
IIPSANIGKDCAIFEAVHGSAPQIAGQNKANPTALIQSSVMMLRYLGEYENAQKIETALEKVFLEGSKLTVDLGGSASTT
EFADEVCKYIV

Sequences:

>Translated_331_residues
MYKVTLIPGDGIGPEVAKAMKKVVEATGVEIEWEEVNAGEAVIEEYGTPLPEYIIDSIKKNKIAIKGPITTPVGKGFRSV
NVTLRQALDLYVNLRPIKSFKGIKSRYEDVDLVVVRENTEDLYAGIEHKIGDYAAESIKIITRSASERIVDFACNYVKDN
KRKKVTAIHKANIMKMSDGLFLDVFREVASKHGVEYDDLIVDAAAMNLVLNPENYDVMVMPNLYGDILSDLGAGLVGGLG
IIPSANIGKDCAIFEAVHGSAPQIAGQNKANPTALIQSSVMMLRYLGEYENAQKIETALEKVFLEGSKLTVDLGGSASTT
EFADEVCKYIV
>Mature_331_residues
MYKVTLIPGDGIGPEVAKAMKKVVEATGVEIEWEEVNAGEAVIEEYGTPLPEYIIDSIKKNKIAIKGPITTPVGKGFRSV
NVTLRQALDLYVNLRPIKSFKGIKSRYEDVDLVVVRENTEDLYAGIEHKIGDYAAESIKIITRSASERIVDFACNYVKDN
KRKKVTAIHKANIMKMSDGLFLDVFREVASKHGVEYDDLIVDAAAMNLVLNPENYDVMVMPNLYGDILSDLGAGLVGGLG
IIPSANIGKDCAIFEAVHGSAPQIAGQNKANPTALIQSSVMMLRYLGEYENAQKIETALEKVFLEGSKLTVDLGGSASTT
EFADEVCKYIV

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=331, Percent_Identity=50.7552870090634, Blast_Score=343, Evalue=1e-94,
Organism=Homo sapiens, GI4758582, Length=333, Percent_Identity=41.7417417417417, Blast_Score=252, Evalue=3e-67,
Organism=Homo sapiens, GI28178821, Length=334, Percent_Identity=42.2155688622754, Blast_Score=249, Evalue=2e-66,
Organism=Homo sapiens, GI28178816, Length=330, Percent_Identity=42.4242424242424, Blast_Score=247, Evalue=1e-65,
Organism=Homo sapiens, GI28178838, Length=302, Percent_Identity=43.3774834437086, Blast_Score=241, Evalue=8e-64,
Organism=Homo sapiens, GI28178819, Length=194, Percent_Identity=45.8762886597938, Blast_Score=160, Evalue=2e-39,
Organism=Escherichia coli, GI1787381, Length=354, Percent_Identity=40.3954802259887, Blast_Score=228, Evalue=4e-61,
Organism=Escherichia coli, GI1788101, Length=356, Percent_Identity=32.0224719101124, Blast_Score=177, Evalue=7e-46,
Organism=Escherichia coli, GI87081683, Length=358, Percent_Identity=32.6815642458101, Blast_Score=162, Evalue=3e-41,
Organism=Caenorhabditis elegans, GI71986051, Length=329, Percent_Identity=48.936170212766, Blast_Score=339, Evalue=9e-94,
Organism=Caenorhabditis elegans, GI25144293, Length=337, Percent_Identity=40.0593471810089, Blast_Score=251, Evalue=5e-67,
Organism=Caenorhabditis elegans, GI17550882, Length=332, Percent_Identity=42.1686746987952, Blast_Score=250, Evalue=7e-67,
Organism=Caenorhabditis elegans, GI17505779, Length=332, Percent_Identity=40.6626506024096, Blast_Score=244, Evalue=4e-65,
Organism=Saccharomyces cerevisiae, GI6324709, Length=338, Percent_Identity=47.6331360946746, Blast_Score=314, Evalue=1e-86,
Organism=Saccharomyces cerevisiae, GI6324291, Length=338, Percent_Identity=43.7869822485207, Blast_Score=280, Evalue=2e-76,
Organism=Saccharomyces cerevisiae, GI6322097, Length=344, Percent_Identity=41.2790697674419, Blast_Score=237, Evalue=2e-63,
Organism=Saccharomyces cerevisiae, GI6319830, Length=358, Percent_Identity=31.8435754189944, Blast_Score=129, Evalue=6e-31,
Organism=Drosophila melanogaster, GI24643268, Length=326, Percent_Identity=53.3742331288344, Blast_Score=360, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24643270, Length=326, Percent_Identity=53.3742331288344, Blast_Score=360, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24661184, Length=334, Percent_Identity=47.6047904191617, Blast_Score=335, Evalue=3e-92,
Organism=Drosophila melanogaster, GI161078635, Length=332, Percent_Identity=39.7590361445783, Blast_Score=240, Evalue=1e-63,
Organism=Drosophila melanogaster, GI161078637, Length=332, Percent_Identity=39.7590361445783, Blast_Score=239, Evalue=1e-63,
Organism=Drosophila melanogaster, GI161078633, Length=330, Percent_Identity=40, Blast_Score=239, Evalue=1e-63,
Organism=Drosophila melanogaster, GI24650122, Length=330, Percent_Identity=40, Blast_Score=239, Evalue=1e-63,
Organism=Drosophila melanogaster, GI161078639, Length=326, Percent_Identity=39.8773006134969, Blast_Score=235, Evalue=3e-62,
Organism=Drosophila melanogaster, GI281362242, Length=329, Percent_Identity=40.7294832826748, Blast_Score=229, Evalue=2e-60,
Organism=Drosophila melanogaster, GI24648872, Length=329, Percent_Identity=40.7294832826748, Blast_Score=229, Evalue=2e-60,
Organism=Drosophila melanogaster, GI20130355, Length=327, Percent_Identity=33.0275229357798, Blast_Score=199, Evalue=3e-51,
Organism=Drosophila melanogaster, GI45550436, Length=322, Percent_Identity=22.9813664596273, Blast_Score=81, Evalue=1e-15,

Paralogues:

None

Copy number: 8180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1480 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR011828 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 36112; Mature: 36112

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYKVTLIPGDGIGPEVAKAMKKVVEATGVEIEWEEVNAGEAVIEEYGTPLPEYIIDSIKK
CEEEEEECCCCCCHHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHCCCCHHHHHHHHCC
NKIAIKGPITTPVGKGFRSVNVTLRQALDLYVNLRPIKSFKGIKSRYEDVDLVVVRENTE
CCEEEECCCCCCCCCCHHHHHHHHHHHHHHHEEEEHHHHHHHHHHHCCCCEEEEEECCCH
DLYAGIEHKIGDYAAESIKIITRSASERIVDFACNYVKDNKRKKVTAIHKANIMKMSDGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEEHHHCCEEEECCCH
FLDVFREVASKHGVEYDDLIVDAAAMNLVLNPENYDVMVMPNLYGDILSDLGAGLVGGLG
HHHHHHHHHHHCCCCHHHHHHHHHHEEEEECCCCCEEEECCHHHHHHHHHHHHHHHCCCC
IIPSANIGKDCAIFEAVHGSAPQIAGQNKANPTALIQSSVMMLRYLGEYENAQKIETALE
CCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
KVFLEGSKLTVDLGGSASTTEFADEVCKYIV
HHHHCCCEEEEEECCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MYKVTLIPGDGIGPEVAKAMKKVVEATGVEIEWEEVNAGEAVIEEYGTPLPEYIIDSIKK
CEEEEEECCCCCCHHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHCCCCHHHHHHHHCC
NKIAIKGPITTPVGKGFRSVNVTLRQALDLYVNLRPIKSFKGIKSRYEDVDLVVVRENTE
CCEEEECCCCCCCCCCHHHHHHHHHHHHHHHEEEEHHHHHHHHHHHCCCCEEEEEECCCH
DLYAGIEHKIGDYAAESIKIITRSASERIVDFACNYVKDNKRKKVTAIHKANIMKMSDGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEEHHHCCEEEECCCH
FLDVFREVASKHGVEYDDLIVDAAAMNLVLNPENYDVMVMPNLYGDILSDLGAGLVGGLG
HHHHHHHHHHHCCCCHHHHHHHHHHEEEEECCCCCEEEECCHHHHHHHHHHHHHHHCCCC
IIPSANIGKDCAIFEAVHGSAPQIAGQNKANPTALIQSSVMMLRYLGEYENAQKIETALE
CCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
KVFLEGSKLTVDLGGSASTTEFADEVCKYIV
HHHHCCCEEEEEECCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]