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The map label for this gene is aksA [H]

Identifier: 126698412

GI number: 126698412

Start: 1008103

End: 1009296

Strand: Direct

Name: aksA [H]

Synonym: CD0832

Alternate gene names: 126698412

Gene position: 1008103-1009296 (Clockwise)

Preceding gene: 126698410

Following gene: 126698413

Centisome position: 23.5

GC content: 31.99

Gene sequence:

>1194_bases
ATGTGCGTTATATCAAAAGATAGAGCAAAGGAAATAAAAATAGTAGATACTACTTTAAGAGATGGAGAGCAAACAGCTGG
GGTTGTATTTGCAAATAGAGAAAAAATAATGATTGCTGAAATGTTAAGTGACCTAGGTGTTGACCAAATTGAAGTTGGTA
TACCTACTATGGGTGGAGATGAAAAAAATGTAATAAAGCACATATGTAGTAGAAATTTAAAATCAGATATAATGGCTTGG
AATAGAGCAGTAATAAAAGATGTTGAAGAATCTATTTCTTGTGGTGTTGATGCAGTAGCTATATCAATATCAGTATCTGA
CATTCATATAGAAAATAAATTGAGAACTTCTAGAGGCTGGGTTTTAGAAAATATGGCTAAAACAGTTGAATTTGCAAAGA
AAAATGGATTATATGTTTCAGTAAATGGAGAAGATGCTTCAAGAGCAGATATAGACTTCTTAACTGAATTTATAAATGTT
GGAAAACAAGCAGGGGCAGATAGATTTAGATACTGTGATACAGTAGGAGTTATGAATCCTTTTTCTATAAAGAATGCAAT
AGAAACTCTTTATGAAAGAACTAATTTTGATATAGAAATGCATACACATAATGACTTTGGGATGGCAACTGCAAATGCAC
TTGCAGGAATAGCAGCAGGAGCAAATTATGTTGGTGTTACAGTAAATGGATTAGGTGAAAGAGCAGGAAATGCAGCATTA
GAAGAAGTTTTAATGGCACTTAAGTGTGTTTATAAATGTGATTTAAATAATATAGATACTAGAAAATTTAGAGGAATATG
TGAGTATGTAGCACAGGCATCAGGAAGAATACTTCCTACATGGAAACCAGTAGTTGGGGATAATATGTTTATACATGAAT
CTGGTATACATGCAGATGGAGCTTTAAAAGACCCACATAACTATGAACCATTTGACCCAAGTGAAGTAAATCTTGAAAGA
AAAATAGTAATAGGTAAACATTCAGGAAGAGCTGCAGTTGTAAATAAGTTGAGTGAATATGAAATGTATATTTCTCCAGA
AAACGCTACCAAGCTATTAAATGCTATAAGAGCTACATCTATAAGATTAAAAAGAAGTTTAATGGATAAAGAAATTCTTC
AATTATATTGTGATATACTAGCACATGAGAAAGGCACAACAGAAGAAGAAGCAGTTAGAGGTTCATATATTTAA

Upstream 100 bases:

>100_bases
AATGAATAAAAAATAAAAAAATAATCATAAGTTAGAGGTATCAATGAAAAATAAGACTTAATGTTTTTAAAATTAAAAGT
ATACTAAGGGGGGCATTAAT

Downstream 100 bases:

>100_bases
GATATAATTGTTTAGGAGGAAAATATGGGAGATAATATAGTTTATAAAATAATTAAAAAACATATAGTAGATGGAGAAGC
TGTTGCTGGAAGTTCAATTG

Product: trans-homoaconitate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase 2; Alpha-isopropylmalate synthase 2 [H]

Number of amino acids: Translated: 397; Mature: 397

Protein sequence:

>397_residues
MCVISKDRAKEIKIVDTTLRDGEQTAGVVFANREKIMIAEMLSDLGVDQIEVGIPTMGGDEKNVIKHICSRNLKSDIMAW
NRAVIKDVEESISCGVDAVAISISVSDIHIENKLRTSRGWVLENMAKTVEFAKKNGLYVSVNGEDASRADIDFLTEFINV
GKQAGADRFRYCDTVGVMNPFSIKNAIETLYERTNFDIEMHTHNDFGMATANALAGIAAGANYVGVTVNGLGERAGNAAL
EEVLMALKCVYKCDLNNIDTRKFRGICEYVAQASGRILPTWKPVVGDNMFIHESGIHADGALKDPHNYEPFDPSEVNLER
KIVIGKHSGRAAVVNKLSEYEMYISPENATKLLNAIRATSIRLKRSLMDKEILQLYCDILAHEKGTTEEEAVRGSYI

Sequences:

>Translated_397_residues
MCVISKDRAKEIKIVDTTLRDGEQTAGVVFANREKIMIAEMLSDLGVDQIEVGIPTMGGDEKNVIKHICSRNLKSDIMAW
NRAVIKDVEESISCGVDAVAISISVSDIHIENKLRTSRGWVLENMAKTVEFAKKNGLYVSVNGEDASRADIDFLTEFINV
GKQAGADRFRYCDTVGVMNPFSIKNAIETLYERTNFDIEMHTHNDFGMATANALAGIAAGANYVGVTVNGLGERAGNAAL
EEVLMALKCVYKCDLNNIDTRKFRGICEYVAQASGRILPTWKPVVGDNMFIHESGIHADGALKDPHNYEPFDPSEVNLER
KIVIGKHSGRAAVVNKLSEYEMYISPENATKLLNAIRATSIRLKRSLMDKEILQLYCDILAHEKGTTEEEAVRGSYI
>Mature_397_residues
MCVISKDRAKEIKIVDTTLRDGEQTAGVVFANREKIMIAEMLSDLGVDQIEVGIPTMGGDEKNVIKHICSRNLKSDIMAW
NRAVIKDVEESISCGVDAVAISISVSDIHIENKLRTSRGWVLENMAKTVEFAKKNGLYVSVNGEDASRADIDFLTEFINV
GKQAGADRFRYCDTVGVMNPFSIKNAIETLYERTNFDIEMHTHNDFGMATANALAGIAAGANYVGVTVNGLGERAGNAAL
EEVLMALKCVYKCDLNNIDTRKFRGICEYVAQASGRILPTWKPVVGDNMFIHESGIHADGALKDPHNYEPFDPSEVNLER
KIVIGKHSGRAAVVNKLSEYEMYISPENATKLLNAIRATSIRLKRSLMDKEILQLYCDILAHEKGTTEEEAVRGSYI

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=362, Percent_Identity=36.1878453038674, Blast_Score=188, Evalue=5e-49,
Organism=Saccharomyces cerevisiae, GI6320071, Length=316, Percent_Identity=34.1772151898734, Blast_Score=164, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6320019, Length=371, Percent_Identity=31.8059299191375, Blast_Score=164, Evalue=2e-41,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR013477
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 43844; Mature: 43844

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCVISKDRAKEIKIVDTTLRDGEQTAGVVFANREKIMIAEMLSDLGVDQIEVGIPTMGGD
CCCCCCCCCCEEEEEEECCCCCCCCCEEEEECCCEEHHHHHHHHCCCCEEEECCCCCCCC
EKNVIKHICSRNLKSDIMAWNRAVIKDVEESISCGVDAVAISISVSDIHIENKLRTSRGW
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEEEEECHHHHCCCCH
VLENMAKTVEFAKKNGLYVSVNGEDASRADIDFLTEFINVGKQAGADRFRYCDTVGVMNP
HHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCC
FSIKNAIETLYERTNFDIEMHTHNDFGMATANALAGIAAGANYVGVTVNGLGERAGNAAL
HHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHH
EEVLMALKCVYKCDLNNIDTRKFRGICEYVAQASGRILPTWKPVVGDNMFIHESGIHADG
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHCCCCEEEEECCCCCCC
ALKDPHNYEPFDPSEVNLERKIVIGKHSGRAAVVNKLSEYEMYISPENATKLLNAIRATS
CCCCCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHCEEEECCCHHHHHHHHHHHHH
IRLKRSLMDKEILQLYCDILAHEKGTTEEEAVRGSYI
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCC
>Mature Secondary Structure
MCVISKDRAKEIKIVDTTLRDGEQTAGVVFANREKIMIAEMLSDLGVDQIEVGIPTMGGD
CCCCCCCCCCEEEEEEECCCCCCCCCEEEEECCCEEHHHHHHHHCCCCEEEECCCCCCCC
EKNVIKHICSRNLKSDIMAWNRAVIKDVEESISCGVDAVAISISVSDIHIENKLRTSRGW
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEEEEECHHHHCCCCH
VLENMAKTVEFAKKNGLYVSVNGEDASRADIDFLTEFINVGKQAGADRFRYCDTVGVMNP
HHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCC
FSIKNAIETLYERTNFDIEMHTHNDFGMATANALAGIAAGANYVGVTVNGLGERAGNAAL
HHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHH
EEVLMALKCVYKCDLNNIDTRKFRGICEYVAQASGRILPTWKPVVGDNMFIHESGIHADG
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHCCCCEEEEECCCCCCC
ALKDPHNYEPFDPSEVNLERKIVIGKHSGRAAVVNKLSEYEMYISPENATKLLNAIRATS
CCCCCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHCEEEECCCHHHHHHHHHHHHH
IRLKRSLMDKEILQLYCDILAHEKGTTEEEAVRGSYI
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]