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The map label for this gene is nadE [H]

Identifier: 126698374

GI number: 126698374

Start: 965121

End: 965876

Strand: Direct

Name: nadE [H]

Synonym: CD0794

Alternate gene names: 126698374

Gene position: 965121-965876 (Clockwise)

Preceding gene: 126698371

Following gene: 126698375

Centisome position: 22.5

GC content: 33.07

Gene sequence:

>756_bases
ATGTCAAATATAAAAATACAAATAGACAAAACTGTAGAATGGCTTATAAATAAGGTTAATGAGGCAAATGCAAAAGGATT
AATTGTTGGTGTATCTGGAGGAATTGATTCGGCAGTAGTTGCTAATTTAATTAAAAAGGCATTTCCAGAAAACTCTATGG
GAGTTATAATGAGCATAAAAAGTAATCCTCAAGATAGAGAAGATGCACTTAAGGTTATAGAGGGATGTGATATAGAATAC
CTTGATTTAGACCTTATAGAACCACAAAGTGCTATATTAGACATGGTAGTAGGAAATTTAAAAGATAAACATTTATATAG
AGAAGAATATTTAAAGATGACAGATGCAAACTTAAGAGCAAGAGTAAGAATGAGTACAATTTATACAATTGCTAACAATC
TAGGATATCTGGTTGTTGGTACAGACAATGCTGCAGAAATACACACAGGATATTTTACTAAATTTGGTGATGGTGGAGTT
GATATTTTACCTATTGCAAATTTAACTAAAGGAGAGGTGTATGAATGGGCAAAAGAGCTTGGTGTTCATGAAGATTTAAT
AAACAAAGCTCCTTCAGCAGGTCTTTGGGAAGGTCAAACAGATGAAGATGAAATGGGAACTACTTACAATATGATAGATG
CTGTGTTAGAAGGTAGATTGGATGAAGTTCCTAAAAGAGACCAAGAGATAATTGAAAGACTTCATAGATTAAGTGAACAC
AAGAGAAAAACGCCTGCACAACCTCCTAAGTTCTAA

Upstream 100 bases:

>100_bases
TGTTTGTTTAATTTTGCATAAAAAAAAACATATTAAAACAACATATTAATATATGGTATAATATATTAAAACAAAGTAGC
AAATTTGAGAGGAGATAACA

Downstream 100 bases:

>100_bases
TTTACTATTTATAAGATTTGTGATAAAATTTACAAGTGATGAAAATTAGAATAATTAATTGTACGTATTAAGGAGTGGAA
ATATGGGACGTATAGGTAAT

Product: NH3-dependent NAD(+) synthetase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 251; Mature: 250

Protein sequence:

>251_residues
MSNIKIQIDKTVEWLINKVNEANAKGLIVGVSGGIDSAVVANLIKKAFPENSMGVIMSIKSNPQDREDALKVIEGCDIEY
LDLDLIEPQSAILDMVVGNLKDKHLYREEYLKMTDANLRARVRMSTIYTIANNLGYLVVGTDNAAEIHTGYFTKFGDGGV
DILPIANLTKGEVYEWAKELGVHEDLINKAPSAGLWEGQTDEDEMGTTYNMIDAVLEGRLDEVPKRDQEIIERLHRLSEH
KRKTPAQPPKF

Sequences:

>Translated_251_residues
MSNIKIQIDKTVEWLINKVNEANAKGLIVGVSGGIDSAVVANLIKKAFPENSMGVIMSIKSNPQDREDALKVIEGCDIEY
LDLDLIEPQSAILDMVVGNLKDKHLYREEYLKMTDANLRARVRMSTIYTIANNLGYLVVGTDNAAEIHTGYFTKFGDGGV
DILPIANLTKGEVYEWAKELGVHEDLINKAPSAGLWEGQTDEDEMGTTYNMIDAVLEGRLDEVPKRDQEIIERLHRLSEH
KRKTPAQPPKF
>Mature_250_residues
SNIKIQIDKTVEWLINKVNEANAKGLIVGVSGGIDSAVVANLIKKAFPENSMGVIMSIKSNPQDREDALKVIEGCDIEYL
DLDLIEPQSAILDMVVGNLKDKHLYREEYLKMTDANLRARVRMSTIYTIANNLGYLVVGTDNAAEIHTGYFTKFGDGGVD
ILPIANLTKGEVYEWAKELGVHEDLINKAPSAGLWEGQTDEDEMGTTYNMIDAVLEGRLDEVPKRDQEIIERLHRLSEHK
RKTPAQPPKF

Specific function: This NAD Synthase Uses Nh(3) In Preference To Glutamine. [C]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1788036, Length=263, Percent_Identity=32.319391634981, Blast_Score=111, Evalue=6e-26,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR022926
- InterPro:   IPR014729 [H]

Pfam domain/function: PF02540 NAD_synthase [H]

EC number: =6.3.1.5 [H]

Molecular weight: Translated: 28012; Mature: 27881

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNIKIQIDKTVEWLINKVNEANAKGLIVGVSGGIDSAVVANLIKKAFPENSMGVIMSIK
CCCEEEEEHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCEEEEEEC
SNPQDREDALKVIEGCDIEYLDLDLIEPQSAILDMVVGNLKDKHLYREEYLKMTDANLRA
CCCCCHHHHHHHHHCCCEEEEECEEECHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHH
RVRMSTIYTIANNLGYLVVGTDNAAEIHTGYFTKFGDGGVDILPIANLTKGEVYEWAKEL
EEHHHHHHHHHCCCCEEEEECCCCCEEECCEEEEECCCCEEEEECCCCCCHHHHHHHHHH
GVHEDLINKAPSAGLWEGQTDEDEMGTTYNMIDAVLEGRLDEVPKRDQEIIERLHRLSEH
CCHHHHHHCCCCCCCCCCCCCCHHCCCHHHHHHHHHHCHHHHCCCHHHHHHHHHHHHHHH
KRKTPAQPPKF
HHCCCCCCCCC
>Mature Secondary Structure 
SNIKIQIDKTVEWLINKVNEANAKGLIVGVSGGIDSAVVANLIKKAFPENSMGVIMSIK
CCEEEEEHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCEEEEEEC
SNPQDREDALKVIEGCDIEYLDLDLIEPQSAILDMVVGNLKDKHLYREEYLKMTDANLRA
CCCCCHHHHHHHHHCCCEEEEECEEECHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHH
RVRMSTIYTIANNLGYLVVGTDNAAEIHTGYFTKFGDGGVDILPIANLTKGEVYEWAKEL
EEHHHHHHHHHCCCCEEEEECCCCCEEECCEEEEECCCCEEEEECCCCCCHHHHHHHHHH
GVHEDLINKAPSAGLWEGQTDEDEMGTTYNMIDAVLEGRLDEVPKRDQEIIERLHRLSEH
CCHHHHHHCCCCCCCCCCCCCCHHCCCHHHHHHHHHHCHHHHCCCHHHHHHHHHHHHHHH
KRKTPAQPPKF
HHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA