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The map label for this gene is sigF [H]

Identifier: 126698352

GI number: 126698352

Start: 943319

End: 944083

Strand: Direct

Name: sigF [H]

Synonym: CD0772

Alternate gene names: 126698352

Gene position: 943319-944083 (Clockwise)

Preceding gene: 126698351

Following gene: 126698353

Centisome position: 21.99

GC content: 30.72

Gene sequence:

>765_bases
ATGGAAGTAACTGTTGCCAGAGAAGAAAAAAAACCTCTTCTTAGTCATGAAGAGACTCTAGAACTTATTGAAAAAGTTCA
AAATGGAGATGAAGAGGCAAAAGAAATATTAATATCAAGCAATTTAGGTCTAGTTAGGAGCGTTGTATCTAGATTTTTAA
ATATAGGATATGATAGAGAAGATTTATTTCAGTTAGGTTCTATAGGCTTAATAAAATCAATATATAAATTTGACCCAAAA
TTTAATGTAAAATTCTCTACTTATGCAGTACCAATGATTTTAGGTGAAATAAAGAGATACTTAAGAGATGATGGTATGAT
AAAAGTATCAAGGTCGTTAAAGCAAATAGCTATAAAGGCAAAGATGGAAAGTGAAGCTTTAACTAAAAAATTAGGTAGAG
AGCCGAGTATAGAAGAATTAGCTAAAGCAATAGATGTAGAAAAAGAAGATTTAGTAATGGCTATGGAAGCTAATTTCAAT
GTTGAGTATTTGCAAGGAGTTATTCATGAAGAGGAAGGTTCTCCAATATGCCTTATTGATAAAATAAGCATGAAAGGAGA
AAGTGAAGAAGAAAAAGTAGTGGATAACATATTGTTAAAAGACATACTTGGAAGACTGGATAAGAGAGAGCGTCAAATAA
TAGTTCTCAGATACTTTGAAGACATGACTCAAAGTGAAATAGGAGAGATGCTCAATATATCACAAGTTCAAGTATCTAGG
ATAGAGAAGAAAGTATTGTCTAAGTTAAAAGAATATATATCGTGA

Upstream 100 bases:

>100_bases
GACGATGTAGAGGTAAGTTCTGTAAAAGATAATGGGACTAGAATTGAAATGACTAAAAAAATAAATGTGCCTAAATAAAG
TCCAAAAGTGAGGTTTTATT

Downstream 100 bases:

>100_bases
TAATACTTATGATATGTAGAATAATAAAATATAATAAATATATTACTAAAATAAAATGTAATAACTTTTTATGTTAAGTT
TTTAAATTATTAAAAGATAT

Product: RNA polymerase sigma-F factor

Products: NA

Alternate protein names: Sporulation sigma factor; Stage II sporulation protein AC [H]

Number of amino acids: Translated: 254; Mature: 254

Protein sequence:

>254_residues
MEVTVAREEKKPLLSHEETLELIEKVQNGDEEAKEILISSNLGLVRSVVSRFLNIGYDREDLFQLGSIGLIKSIYKFDPK
FNVKFSTYAVPMILGEIKRYLRDDGMIKVSRSLKQIAIKAKMESEALTKKLGREPSIEELAKAIDVEKEDLVMAMEANFN
VEYLQGVIHEEEGSPICLIDKISMKGESEEEKVVDNILLKDILGRLDKRERQIIVLRYFEDMTQSEIGEMLNISQVQVSR
IEKKVLSKLKEYIS

Sequences:

>Translated_254_residues
MEVTVAREEKKPLLSHEETLELIEKVQNGDEEAKEILISSNLGLVRSVVSRFLNIGYDREDLFQLGSIGLIKSIYKFDPK
FNVKFSTYAVPMILGEIKRYLRDDGMIKVSRSLKQIAIKAKMESEALTKKLGREPSIEELAKAIDVEKEDLVMAMEANFN
VEYLQGVIHEEEGSPICLIDKISMKGESEEEKVVDNILLKDILGRLDKRERQIIVLRYFEDMTQSEIGEMLNISQVQVSR
IEKKVLSKLKEYIS
>Mature_254_residues
MEVTVAREEKKPLLSHEETLELIEKVQNGDEEAKEILISSNLGLVRSVVSRFLNIGYDREDLFQLGSIGLIKSIYKFDPK
FNVKFSTYAVPMILGEIKRYLRDDGMIKVSRSLKQIAIKAKMESEALTKKLGREPSIEELAKAIDVEKEDLVMAMEANFN
VEYLQGVIHEEEGSPICLIDKISMKGESEEEKVVDNILLKDILGRLDKRERQIIVLRYFEDMTQSEIGEMLNISQVQVSR
IEKKVLSKLKEYIS

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of sporulation specific genes. Interaction with spoIIAB inhibits sig

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789098, Length=257, Percent_Identity=26.0700389105058, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1789871, Length=260, Percent_Identity=26.9230769230769, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1789448, Length=229, Percent_Identity=27.0742358078603, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1788231, Length=195, Percent_Identity=26.1538461538462, Blast_Score=65, Evalue=5e-12,

Paralogues:

None

Copy number: <10 (log phase) 250 (stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014322
- InterPro:   IPR014236
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 29115; Mature: 29115

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEVTVAREEKKPLLSHEETLELIEKVQNGDEEAKEILISSNLGLVRSVVSRFLNIGYDRE
CCCCCCCHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCHH
DLFQLGSIGLIKSIYKFDPKFNVKFSTYAVPMILGEIKRYLRDDGMIKVSRSLKQIAIKA
HHHHHCCHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
KMESEALTKKLGREPSIEELAKAIDVEKEDLVMAMEANFNVEYLQGVIHEEEGSPICLID
HHHHHHHHHHHCCCCCHHHHHHHHCCCHHHEEEEEECCCCHHHHHHHHHCCCCCCEEEEE
KISMKGESEEEKVVDNILLKDILGRLDKRERQIIVLRYFEDMTQSEIGEMLNISQVQVSR
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHH
IEKKVLSKLKEYIS
HHHHHHHHHHHHHC
>Mature Secondary Structure
MEVTVAREEKKPLLSHEETLELIEKVQNGDEEAKEILISSNLGLVRSVVSRFLNIGYDRE
CCCCCCCHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCHH
DLFQLGSIGLIKSIYKFDPKFNVKFSTYAVPMILGEIKRYLRDDGMIKVSRSLKQIAIKA
HHHHHCCHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
KMESEALTKKLGREPSIEELAKAIDVEKEDLVMAMEANFNVEYLQGVIHEEEGSPICLID
HHHHHHHHHHHCCCCCHHHHHHHHCCCHHHEEEEEECCCCHHHHHHHHHCCCCCCEEEEE
KISMKGESEEEKVVDNILLKDILGRLDKRERQIIVLRYFEDMTQSEIGEMLNISQVQVSR
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHH
IEKKVLSKLKEYIS
HHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3116160; 6088674; 8969508; 9384377; 8358793 [H]