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The map label for this gene is thiI

Identifier: 126698334

GI number: 126698334

Start: 921942

End: 923117

Strand: Direct

Name: thiI

Synonym: CD0754

Alternate gene names: 126698334

Gene position: 921942-923117 (Clockwise)

Preceding gene: 126698333

Following gene: 126698335

Centisome position: 21.49

GC content: 32.57

Gene sequence:

>1176_bases
ATGGTGTATAATATATTAATAGTAAAGTATGGAGAAATTGGAGTAAAAGGAAAGAATAGATATATATTTGAGAATAGGCT
TATAAGAAATATAAGAAATATGTTAAAACCAATTGGAAGATTTAATGTATATAAAGAGTATGGAAGAATATATGTTGATT
TAGAAGACTATGATTATGAAGAAGTAATTGAAGAAGTTAGAAAAGTATTTGGGATAGTGGGTGTGTGCCCTGGAGTAAGA
GCTAAAAAAGATTATGATACATTAAAAGAAATAGCTTTAAAAATGTTAGAAGAAAAAATAGAGGCTGGATATAAAACTTT
TAAGGTAGAGTCAAGAAGAGGAGATAAGTCTTTCGGGTTGACTTCTCAAGAAATGAGTATGGATATAGGTGGATACTTAT
TATCAAAGGTAGGAGATAGGATTAATGTTGATGTAAGAAATCCAGAAGTTAAAATAAAGTGCGAATACAGAGAATTTCAT
ACTATGGTCTATAGTGATACTATACCTGGATATGGTGGATTGCCACTTGGAACGAATGGTAGAGCTATGTCTCTTTTATC
AGGAGGTATAGATAGCCCAGTGGCTACATGGATGGTTGCAAAAAGAGGTATGGAAGTAGAGGCAGTTCACTTTCATAGTT
ATCCATTTACAAGTGAAAGGTCACAGGAAAAAGTTAAGGATTTAGCTAAAATACTGGCAAAATACTGTGGTAGAGTAAGG
CTTCATAAAGTTAATATCTTAGAAATACAAAAAGCAATTGGTGAGAATTGTAATGAAGAAGAAGCTACTATTCTTTCAAG
AAGATTTATGATGAGGATAGCACAGAGACTTTCTGAAAAAAGACATTGTGATGCTTTAATTACAGGTGAAAGTATTGGGC
AGGTTGCATCTCAAACTATACAAGGGCTTACTTGTACTAATGCCGTTGTAGACTTACCAGTGTTTAGACCACTTATAGCA
ATGGATAAATCAGATATAGTTGATATAGCCAAAAAAATAGGGACTTTTGAAACATCTATAGTGCCTGAAGAAGATTGTTG
TAGTGTATTTTCTCCTAGAAAGCCCGTCACTAAACCTAGATTAGAGAAAATAGAAAAATCTGAAACAGCACTTGACATTG
AAAAATTAGTACAGGATGTTATTGACAAAATAGAGGTAGAGGATATTGAGTTTTAG

Upstream 100 bases:

>100_bases
GAAAGTTTTGAAAGAATCAATTTGTGACTTAAGGTCAATAATGAAAAGAAAATAAAGAATACTGTATAAATAGTGATAAT
AAATTAGTTTAAGAGGTGAA

Downstream 100 bases:

>100_bases
CATACAAGTTATTTAATTACTAAATTGAATATTGAGTATATTAGTAGTTCTGTAAGTATATTAGTAGTTCTGTTGAAAGG
ATTTATGTAGACAATTTATT

Product: thiamine biosynthesis protein ThiI

Products: NA

Alternate protein names: Sulfur carrier protein ThiS sulfurtransferase; Thiamine biosynthesis protein thiI; tRNA 4-thiouridine synthase

Number of amino acids: Translated: 391; Mature: 391

Protein sequence:

>391_residues
MVYNILIVKYGEIGVKGKNRYIFENRLIRNIRNMLKPIGRFNVYKEYGRIYVDLEDYDYEEVIEEVRKVFGIVGVCPGVR
AKKDYDTLKEIALKMLEEKIEAGYKTFKVESRRGDKSFGLTSQEMSMDIGGYLLSKVGDRINVDVRNPEVKIKCEYREFH
TMVYSDTIPGYGGLPLGTNGRAMSLLSGGIDSPVATWMVAKRGMEVEAVHFHSYPFTSERSQEKVKDLAKILAKYCGRVR
LHKVNILEIQKAIGENCNEEEATILSRRFMMRIAQRLSEKRHCDALITGESIGQVASQTIQGLTCTNAVVDLPVFRPLIA
MDKSDIVDIAKKIGTFETSIVPEEDCCSVFSPRKPVTKPRLEKIEKSETALDIEKLVQDVIDKIEVEDIEF

Sequences:

>Translated_391_residues
MVYNILIVKYGEIGVKGKNRYIFENRLIRNIRNMLKPIGRFNVYKEYGRIYVDLEDYDYEEVIEEVRKVFGIVGVCPGVR
AKKDYDTLKEIALKMLEEKIEAGYKTFKVESRRGDKSFGLTSQEMSMDIGGYLLSKVGDRINVDVRNPEVKIKCEYREFH
TMVYSDTIPGYGGLPLGTNGRAMSLLSGGIDSPVATWMVAKRGMEVEAVHFHSYPFTSERSQEKVKDLAKILAKYCGRVR
LHKVNILEIQKAIGENCNEEEATILSRRFMMRIAQRLSEKRHCDALITGESIGQVASQTIQGLTCTNAVVDLPVFRPLIA
MDKSDIVDIAKKIGTFETSIVPEEDCCSVFSPRKPVTKPRLEKIEKSETALDIEKLVQDVIDKIEVEDIEF
>Mature_391_residues
MVYNILIVKYGEIGVKGKNRYIFENRLIRNIRNMLKPIGRFNVYKEYGRIYVDLEDYDYEEVIEEVRKVFGIVGVCPGVR
AKKDYDTLKEIALKMLEEKIEAGYKTFKVESRRGDKSFGLTSQEMSMDIGGYLLSKVGDRINVDVRNPEVKIKCEYREFH
TMVYSDTIPGYGGLPLGTNGRAMSLLSGGIDSPVATWMVAKRGMEVEAVHFHSYPFTSERSQEKVKDLAKILAKYCGRVR
LHKVNILEIQKAIGENCNEEEATILSRRFMMRIAQRLSEKRHCDALITGESIGQVASQTIQGLTCTNAVVDLPVFRPLIA
MDKSDIVDIAKKIGTFETSIVPEEDCCSVFSPRKPVTKPRLEKIEKSETALDIEKLVQDVIDKIEVEDIEF

Specific function: Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. Thi

COG id: COG0301

COG function: function code H; Thiamine biosynthesis ATP pyrophosphatase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain

Homologues:

Organism=Escherichia coli, GI1786625, Length=387, Percent_Identity=29.4573643410853, Blast_Score=133, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THII_CLOD6 (Q189U8)

Other databases:

- EMBL:   AM180355
- RefSeq:   YP_001087231.1
- ProteinModelPortal:   Q189U8
- SMR:   Q189U8
- STRING:   Q189U8
- GeneID:   4915328
- GenomeReviews:   AM180355_GR
- KEGG:   cdf:CD0754
- NMPDR:   fig|1496.1.peg.1894
- eggNOG:   COG0301
- HOGENOM:   HBG646072
- OMA:   ESIGQVA
- ProtClustDB:   PRK01565
- GO:   GO:0005737
- HAMAP:   MF_00021
- InterPro:   IPR014729
- InterPro:   IPR003720
- InterPro:   IPR020536
- InterPro:   IPR004114
- Gene3D:   G3DSA:3.40.50.620
- SMART:   SM00981
- TIGRFAMs:   TIGR00342

Pfam domain/function: PF02568 ThiI; PF02926 THUMP

EC number: =2.8.1.4

Molecular weight: Translated: 44465; Mature: 44465

Theoretical pI: Translated: 7.36; Mature: 7.36

Prosite motif: PS51165 THUMP

Important sites: BINDING 267-267 BINDING 289-289 BINDING 298-298

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVYNILIVKYGEIGVKGKNRYIFENRLIRNIRNMLKPIGRFNVYKEYGRIYVDLEDYDYE
CEEEEEEEEECCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCHH
EVIEEVRKVFGIVGVCPGVRAKKDYDTLKEIALKMLEEKIEAGYKTFKVESRRGDKSFGL
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCC
TSQEMSMDIGGYLLSKVGDRINVDVRNPEVKIKCEYREFHTMVYSDTIPGYGGLPLGTNG
CHHHHHHHHHHHHHHHCCCEEEEEEECCEEEEEEEHHHHHHHHEECCCCCCCCCCCCCCC
RAMSLLSGGIDSPVATWMVAKRGMEVEAVHFHSYPFTSERSQEKVKDLAKILAKYCGRVR
CCHHHHHCCCCCHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCEE
LHKVNILEIQKAIGENCNEEEATILSRRFMMRIAQRLSEKRHCDALITGESIGQVASQTI
EEECHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCHHHHHHHHHHH
QGLTCTNAVVDLPVFRPLIAMDKSDIVDIAKKIGTFETSIVPEEDCCSVFSPRKPVTKPR
CCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHCCCCCHHHHHHCCCCCCCCCHH
LEKIEKSETALDIEKLVQDVIDKIEVEDIEF
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MVYNILIVKYGEIGVKGKNRYIFENRLIRNIRNMLKPIGRFNVYKEYGRIYVDLEDYDYE
CEEEEEEEEECCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCHH
EVIEEVRKVFGIVGVCPGVRAKKDYDTLKEIALKMLEEKIEAGYKTFKVESRRGDKSFGL
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCC
TSQEMSMDIGGYLLSKVGDRINVDVRNPEVKIKCEYREFHTMVYSDTIPGYGGLPLGTNG
CHHHHHHHHHHHHHHHCCCEEEEEEECCEEEEEEEHHHHHHHHEECCCCCCCCCCCCCCC
RAMSLLSGGIDSPVATWMVAKRGMEVEAVHFHSYPFTSERSQEKVKDLAKILAKYCGRVR
CCHHHHHCCCCCHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCEE
LHKVNILEIQKAIGENCNEEEATILSRRFMMRIAQRLSEKRHCDALITGESIGQVASQTI
EEECHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCHHHHHHHHHHH
QGLTCTNAVVDLPVFRPLIAMDKSDIVDIAKKIGTFETSIVPEEDCCSVFSPRKPVTKPR
CCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHCCCCCHHHHHHCCCCCCCCCHH
LEKIEKSETALDIEKLVQDVIDKIEVEDIEF
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA