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The map label for this gene is topB [H]

Identifier: 126697992

GI number: 126697992

Start: 493445

End: 495151

Strand: Direct

Name: topB [H]

Synonym: CD0421

Alternate gene names: 126697992

Gene position: 493445-495151 (Clockwise)

Preceding gene: 126697991

Following gene: 126697993

Centisome position: 11.5

GC content: 36.85

Gene sequence:

>1707_bases
ATGAAGTTAGTTATTGCAGAAAAGCCGAGTGTTGCAATCTCCATTGCAAAGGTTATTGGAGTAAATAAAAAGAAAGACGG
ATATTATGAAGGAAACGGATATAGGGTGAGCTGGTGCGTTGGACACCTAATTCAAATGGCAAATCCGGATGCTTATGATG
AAAAGTACGCCAAGTGGAATATGGCTGATTTACCGATTATTCCGAGCGCCTACAAGTATGAAGTAGCAAAGGCAACCAAG
AAGCAGTTTAACATCCTTAAAAAGCTGATGAATGATAAAGAGATTGATACGGTTATCAACGCTTGTGATGCAGGTCGTGA
AGGAGAAAGCATTTTTAGATTGGTATATAACGAAGCTAAATGCAAAAAGAAGATGAAACGCCTTTGGATTTCATCTATGG
AGGATAGTGCCATTAAAGAGGGCTTTGATAATCTAAAAGACGGAAAGGGCTATGACAATCTCTTTGAATCGGCACAGGCA
AGAGCGATTGCAGATTGGCTTGTCGGAATGAACATCAGCAGGCTCTATTCTTGCCTATATAAGCAAAATTACAGTGTAGG
AAGAGTGCAGACACCGACTCTTGCCATGATTGTAAAAAGAGATGATGAGATAGCTAATTTCAAGAAAGAGAAATACTATA
CAGTGGAGCTATCTATGAATGGTTTTACACTATCAACGGATAGAATTGATGATGAGGTTGCTGCCGAACAGCTAATCAAT
TTAGTTGGCGATAAGATTGAAATCACTGATGTTATTCAGAAAGAGAAAATAACAAATCCTGATTTACCTTTTGACCTGAC
AACACTTCAAAGGGAATGCAATAAGTATTTCGGATATAGTGCCAAGCAGACACTTGACTATGCTCAAAGCCTGTATGAAA
AGAAACTGATTACTTATCCAAGAACGGACAGCAGGTGTTTAACAGAGGATATGATTACAAGTACCATCAATAACATTTTG
GGCAAAAATGACTTTGATACAGGGCGCATCAAGACGGTATTTAACTCTAAAAAGGTTACGGATCACCACGCTATTATTCC
GACAATCAGTTCATTAAAGGAAGATGTTTCTAAGCTTCCTCTAAGCGAAGCTAAAGTTTACTTTCTTATATCCGATAAAT
TTCACGCAAGTGTAGGTTATCCACTTATTGAAAATACAACAAAGATTGTAGCTTCATTTGACGGTTTTGAATTTACAAGT
TCAGGCAAGGTAATTAAGGATGAAGGCTTTAGCAAATACCTTAAAGAATACAAATCAAAAAAGAATGAAGATGCTGTACT
TCCTAATGTAAGTGTTGGTGATGTTTTAAGCATTGAAAATAAAGAGATTAAAGAAAAATTTACCAAACCTCCAAAACACT
TTACGGAAGATACTCTCTTAAAGTCTATGGAGATTGCGGGAAATGATGCCTTAGAAAAAGGTGTAGAAGTGGAAAGAAAA
GGACTTGGAACACCTGCAACAAGGGCAGGGATTATCGAAAATCTAATTTATAAGGGATTTGTAGAAAGAGATAAGAAAAA
TCTTATAGCCACTCATAAAGGAATTAGCCTTGTAACGATTGTAGCGGATAACTTTAAGTCGGCAGAAACGACAGCGAAGT
GGGAAATGGAATTATCTGATATTGCTCAGGGTAAATCATCTAAGAAAGAATTTTTAGATGCGATTGAAAATGAGATAAAA
GAAGCGGTTTTGACATATCGCAAGTAA

Upstream 100 bases:

>100_bases
GAATAAACGCACCTAAAAACATATTATTTTGGGTGCAAAGTTAGGGCGGGAGAGTAACATCTTTCGCCCTATTTTGATTT
ATAACAGGAGGAAAACACAG

Downstream 100 bases:

>100_bases
GACAGCACGAATTTCAGCGTGGAAAAAATGCTCTAAAAATGCTATAATGCAAAGTAATCAATTTAATGATTGAGAGGTGA
TTATATGTCTAAAATAAATG

Product: DNA topoisomerase

Products: NA

Alternate protein names: DNA topoisomerase III [H]

Number of amino acids: Translated: 568; Mature: 568

Protein sequence:

>568_residues
MKLVIAEKPSVAISIAKVIGVNKKKDGYYEGNGYRVSWCVGHLIQMANPDAYDEKYAKWNMADLPIIPSAYKYEVAKATK
KQFNILKKLMNDKEIDTVINACDAGREGESIFRLVYNEAKCKKKMKRLWISSMEDSAIKEGFDNLKDGKGYDNLFESAQA
RAIADWLVGMNISRLYSCLYKQNYSVGRVQTPTLAMIVKRDDEIANFKKEKYYTVELSMNGFTLSTDRIDDEVAAEQLIN
LVGDKIEITDVIQKEKITNPDLPFDLTTLQRECNKYFGYSAKQTLDYAQSLYEKKLITYPRTDSRCLTEDMITSTINNIL
GKNDFDTGRIKTVFNSKKVTDHHAIIPTISSLKEDVSKLPLSEAKVYFLISDKFHASVGYPLIENTTKIVASFDGFEFTS
SGKVIKDEGFSKYLKEYKSKKNEDAVLPNVSVGDVLSIENKEIKEKFTKPPKHFTEDTLLKSMEIAGNDALEKGVEVERK
GLGTPATRAGIIENLIYKGFVERDKKNLIATHKGISLVTIVADNFKSAETTAKWEMELSDIAQGKSSKKEFLDAIENEIK
EAVLTYRK

Sequences:

>Translated_568_residues
MKLVIAEKPSVAISIAKVIGVNKKKDGYYEGNGYRVSWCVGHLIQMANPDAYDEKYAKWNMADLPIIPSAYKYEVAKATK
KQFNILKKLMNDKEIDTVINACDAGREGESIFRLVYNEAKCKKKMKRLWISSMEDSAIKEGFDNLKDGKGYDNLFESAQA
RAIADWLVGMNISRLYSCLYKQNYSVGRVQTPTLAMIVKRDDEIANFKKEKYYTVELSMNGFTLSTDRIDDEVAAEQLIN
LVGDKIEITDVIQKEKITNPDLPFDLTTLQRECNKYFGYSAKQTLDYAQSLYEKKLITYPRTDSRCLTEDMITSTINNIL
GKNDFDTGRIKTVFNSKKVTDHHAIIPTISSLKEDVSKLPLSEAKVYFLISDKFHASVGYPLIENTTKIVASFDGFEFTS
SGKVIKDEGFSKYLKEYKSKKNEDAVLPNVSVGDVLSIENKEIKEKFTKPPKHFTEDTLLKSMEIAGNDALEKGVEVERK
GLGTPATRAGIIENLIYKGFVERDKKNLIATHKGISLVTIVADNFKSAETTAKWEMELSDIAQGKSSKKEFLDAIENEIK
EAVLTYRK
>Mature_568_residues
MKLVIAEKPSVAISIAKVIGVNKKKDGYYEGNGYRVSWCVGHLIQMANPDAYDEKYAKWNMADLPIIPSAYKYEVAKATK
KQFNILKKLMNDKEIDTVINACDAGREGESIFRLVYNEAKCKKKMKRLWISSMEDSAIKEGFDNLKDGKGYDNLFESAQA
RAIADWLVGMNISRLYSCLYKQNYSVGRVQTPTLAMIVKRDDEIANFKKEKYYTVELSMNGFTLSTDRIDDEVAAEQLIN
LVGDKIEITDVIQKEKITNPDLPFDLTTLQRECNKYFGYSAKQTLDYAQSLYEKKLITYPRTDSRCLTEDMITSTINNIL
GKNDFDTGRIKTVFNSKKVTDHHAIIPTISSLKEDVSKLPLSEAKVYFLISDKFHASVGYPLIENTTKIVASFDGFEFTS
SGKVIKDEGFSKYLKEYKSKKNEDAVLPNVSVGDVLSIENKEIKEKFTKPPKHFTEDTLLKSMEIAGNDALEKGVEVERK
GLGTPATRAGIIENLIYKGFVERDKKNLIATHKGISLVTIVADNFKSAETTAKWEMELSDIAQGKSSKKEFLDAIENEIK
EAVLTYRK

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another. TOP3 is a potent decatenase [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI4507635, Length=615, Percent_Identity=24.8780487804878, Blast_Score=122, Evalue=9e-28,
Organism=Homo sapiens, GI10835218, Length=601, Percent_Identity=22.1297836938436, Blast_Score=82, Evalue=1e-15,
Organism=Escherichia coli, GI1788061, Length=600, Percent_Identity=33.6666666666667, Blast_Score=314, Evalue=9e-87,
Organism=Escherichia coli, GI1787529, Length=591, Percent_Identity=26.5651438240271, Blast_Score=162, Evalue=8e-41,
Organism=Caenorhabditis elegans, GI32563869, Length=620, Percent_Identity=25, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17555378, Length=626, Percent_Identity=23.961661341853, Blast_Score=95, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6323263, Length=432, Percent_Identity=25.462962962963, Blast_Score=102, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24640096, Length=608, Percent_Identity=25, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24585251, Length=603, Percent_Identity=22.7197346600332, Blast_Score=86, Evalue=6e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR005738
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 64207; Mature: 64207

Theoretical pI: Translated: 8.27; Mature: 8.27

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLVIAEKPSVAISIAKVIGVNKKKDGYYEGNGYRVSWCVGHLIQMANPDAYDEKYAKWN
CEEEEECCCCHHEEHHHHHCCCCCCCCCCCCCCEEEHHHHHHHHHHCCCCHHHHHHHCCC
MADLPIIPSAYKYEVAKATKKQFNILKKLMNDKEIDTVINACDAGREGESIFRLVYNEAK
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHH
CKKKMKRLWISSMEDSAIKEGFDNLKDGKGYDNLFESAQARAIADWLVGMNISRLYSCLY
HHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
KQNYSVGRVQTPTLAMIVKRDDEIANFKKEKYYTVELSMNGFTLSTDRIDDEVAAEQLIN
HCCCCCCCCCCCEEEEEEECCHHHHCCCCCEEEEEEEEECCEEEECCCCCHHHHHHHHHH
LVGDKIEITDVIQKEKITNPDLPFDLTTLQRECNKYFGYSAKQTLDYAQSLYEKKLITYP
HHCCCEEHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCC
RTDSRCLTEDMITSTINNILGKNDFDTGRIKTVFNSKKVTDHHAIIPTISSLKEDVSKLP
CCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHCC
LSEAKVYFLISDKFHASVGYPLIENTTKIVASFDGFEFTSSGKVIKDEGFSKYLKEYKSK
CCCCEEEEEEECCCCCCCCCCHHCCCCEEEEECCCCEECCCCCEEECCCHHHHHHHHHCC
KNEDAVLPNVSVGDVLSIENKEIKEKFTKPPKHFTEDTLLKSMEIAGNDALEKGVEVERK
CCCCCCCCCCCCCCEEECCCHHHHHHHCCCCCCCCHHHHHHHHHHCCCHHHHHCCHHHHH
GLGTPATRAGIIENLIYKGFVERDKKNLIATHKGISLVTIVADNFKSAETTAKWEMELSD
CCCCCHHHHHHHHHHHHHHHHHHHHHHHEEECCCCEEEEEEECCCCCCCCCHHEEEEHHH
IAQGKSSKKEFLDAIENEIKEAVLTYRK
HHCCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKLVIAEKPSVAISIAKVIGVNKKKDGYYEGNGYRVSWCVGHLIQMANPDAYDEKYAKWN
CEEEEECCCCHHEEHHHHHCCCCCCCCCCCCCCEEEHHHHHHHHHHCCCCHHHHHHHCCC
MADLPIIPSAYKYEVAKATKKQFNILKKLMNDKEIDTVINACDAGREGESIFRLVYNEAK
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHH
CKKKMKRLWISSMEDSAIKEGFDNLKDGKGYDNLFESAQARAIADWLVGMNISRLYSCLY
HHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
KQNYSVGRVQTPTLAMIVKRDDEIANFKKEKYYTVELSMNGFTLSTDRIDDEVAAEQLIN
HCCCCCCCCCCCEEEEEEECCHHHHCCCCCEEEEEEEEECCEEEECCCCCHHHHHHHHHH
LVGDKIEITDVIQKEKITNPDLPFDLTTLQRECNKYFGYSAKQTLDYAQSLYEKKLITYP
HHCCCEEHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCC
RTDSRCLTEDMITSTINNILGKNDFDTGRIKTVFNSKKVTDHHAIIPTISSLKEDVSKLP
CCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHCC
LSEAKVYFLISDKFHASVGYPLIENTTKIVASFDGFEFTSSGKVIKDEGFSKYLKEYKSK
CCCCEEEEEEECCCCCCCCCCHHCCCCEEEEECCCCEECCCCCEEECCCHHHHHHHHHCC
KNEDAVLPNVSVGDVLSIENKEIKEKFTKPPKHFTEDTLLKSMEIAGNDALEKGVEVERK
CCCCCCCCCCCCCCEEECCCHHHHHHHCCCCCCCCHHHHHHHHHHCCCHHHHHCCHHHHH
GLGTPATRAGIIENLIYKGFVERDKKNLIATHKGISLVTIVADNFKSAETTAKWEMELSD
CCCCCHHHHHHHHHHHHHHHHHHHHHHHEEECCCCEEEEEEECCCCCCCCCHHEEEEHHH
IAQGKSSKKEFLDAIENEIKEAVLTYRK
HHCCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA