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The map label for this gene is ytzG [H]

Identifier: 126697974

GI number: 126697974

Start: 478328

End: 479050

Strand: Direct

Name: ytzG [H]

Synonym: CD0405

Alternate gene names: 126697974

Gene position: 478328-479050 (Clockwise)

Preceding gene: 126697973

Following gene: 126697975

Centisome position: 11.15

GC content: 29.88

Gene sequence:

>723_bases
ATGGCTAAAAAGCAACGTATAGATAAAATTCTTTCAAATTTAGGTTATGGAAGTAGAAGTGAAATAAAGAAGTATTGTAA
ACAAGGGTCTGTAGTAGTAAATGGAAGTGAAGTTTCAAATCCAGGAACTCAAGTAGACACTGAAAATGATGAGATATTAT
TTAATGGAGAAGAAGTTATATATAGAGAATATATATATTTGATGATGAATAAACCTGATGGATATATATCAGCAACTACA
GATAAATATGACCCAACAGTATTAGATTTAATTGATTTATCATACTTAGCTTTTGAACCATTTCCTGTTGGAAGGTTAGA
TAAAGACACAGAGGGATTATTAGTTTTAACTAATGATGGAAAGCTTTCTCATAGAGTTTTATCTCCTAAGAAGCATGTGC
CTAAGACTTATTATGCAAAAATTGATGGAGTAGTTACTGAAGAAGATGTAGAAGCTTTTTTAGAAGGTGTAGTGCTTGAT
GACAGATATAAAACTATGCCATCACAGTTAAATATATTAAAAAGTGATGATGAATCAGAAATAGAGCTTACAATACATGA
AGGTAAGTTTCACCAAGTAAAAAGAATGTTTGAAAGCGTAGGCAAAAAAGTAGTATACTTAAAAAGATTGTCTATGGGCA
ATTTAAAATTGGATGAAAGTTTAGAGTTAGGTGAGTATAGAGAGTTAACAGATGAAGAAGTAAAATTGATTGAAGAAAGA
TAA

Upstream 100 bases:

>100_bases
TAACTGATAGTATAAGAAATAATTTAGATGTAGAAAGTATGGATTTAGCATTTACAGTATTTGAAAAAGAAAAATATTAT
GAGAATGGAGAGCATTTTTA

Downstream 100 bases:

>100_bases
TATAGTAAATTGGAGGAATGTCTGTGAAGAAAAAAGATGTTATAGAATTTGAAGTAGATAAAATGGAATTTGGTGGTACA
TCTTTAAGCCAAGTTGGAGA

Product: RNA pseudouridine synthase

Products: pseudouridine 5'-phosphate; H2O [C]

Alternate protein names: RNA pseudouridylate synthase; RNA-uridine isomerase [H]

Number of amino acids: Translated: 240; Mature: 239

Protein sequence:

>240_residues
MAKKQRIDKILSNLGYGSRSEIKKYCKQGSVVVNGSEVSNPGTQVDTENDEILFNGEEVIYREYIYLMMNKPDGYISATT
DKYDPTVLDLIDLSYLAFEPFPVGRLDKDTEGLLVLTNDGKLSHRVLSPKKHVPKTYYAKIDGVVTEEDVEAFLEGVVLD
DRYKTMPSQLNILKSDDESEIELTIHEGKFHQVKRMFESVGKKVVYLKRLSMGNLKLDESLELGEYRELTDEEVKLIEER

Sequences:

>Translated_240_residues
MAKKQRIDKILSNLGYGSRSEIKKYCKQGSVVVNGSEVSNPGTQVDTENDEILFNGEEVIYREYIYLMMNKPDGYISATT
DKYDPTVLDLIDLSYLAFEPFPVGRLDKDTEGLLVLTNDGKLSHRVLSPKKHVPKTYYAKIDGVVTEEDVEAFLEGVVLD
DRYKTMPSQLNILKSDDESEIELTIHEGKFHQVKRMFESVGKKVVYLKRLSMGNLKLDESLELGEYRELTDEEVKLIEER
>Mature_239_residues
AKKQRIDKILSNLGYGSRSEIKKYCKQGSVVVNGSEVSNPGTQVDTENDEILFNGEEVIYREYIYLMMNKPDGYISATTD
KYDPTVLDLIDLSYLAFEPFPVGRLDKDTEGLLVLTNDGKLSHRVLSPKKHVPKTYYAKIDGVVTEEDVEAFLEGVVLDD
RYKTMPSQLNILKSDDESEIELTIHEGKFHQVKRMFESVGKKVVYLKRLSMGNLKLDESLELGEYRELTDEEVKLIEER

Specific function: Responsible For Synthesis Of Pseudouridine From Uracil-516 In 16s Ribosomal RNA. [C]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788510, Length=234, Percent_Identity=37.1794871794872, Blast_Score=152, Evalue=2e-38,
Organism=Escherichia coli, GI1790453, Length=242, Percent_Identity=33.0578512396694, Blast_Score=106, Evalue=1e-24,
Organism=Escherichia coli, GI87081838, Length=147, Percent_Identity=38.0952380952381, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI1787524, Length=236, Percent_Identity=30.0847457627119, Blast_Score=97, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: 4.2.1.70 [C]

Molecular weight: Translated: 27443; Mature: 27311

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKKQRIDKILSNLGYGSRSEIKKYCKQGSVVVNGSEVSNPGTQVDTENDEILFNGEEVI
CCHHHHHHHHHHHCCCCCHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCEEEECCCHHE
YREYIYLMMNKPDGYISATTDKYDPTVLDLIDLSYLAFEPFPVGRLDKDTEGLLVLTNDG
EEEEEEEEEECCCCEEEECCCCCCCHHHHHHHHHHHEECCCCCCCCCCCCCCEEEEECCC
KLSHRVLSPKKHVPKTYYAKIDGVVTEEDVEAFLEGVVLDDRYKTMPSQLNILKSDDESE
CCCHHCCCCHHHCCCCEEEEECCEECHHHHHHHHHCCEECCHHHHCHHHHEEECCCCCCE
IELTIHEGKFHQVKRMFESVGKKVVYLKRLSMGNLKLDESLELGEYRELTDEEVKLIEER
EEEEEECCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCHHHHCCHHHHHHHCCC
>Mature Secondary Structure 
AKKQRIDKILSNLGYGSRSEIKKYCKQGSVVVNGSEVSNPGTQVDTENDEILFNGEEVI
CHHHHHHHHHHHCCCCCHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCEEEECCCHHE
YREYIYLMMNKPDGYISATTDKYDPTVLDLIDLSYLAFEPFPVGRLDKDTEGLLVLTNDG
EEEEEEEEEECCCCEEEECCCCCCCHHHHHHHHHHHEECCCCCCCCCCCCCCEEEEECCC
KLSHRVLSPKKHVPKTYYAKIDGVVTEEDVEAFLEGVVLDDRYKTMPSQLNILKSDDESE
CCCHHCCCCHHHCCCCEEEEECCEECHHHHHHHHHCCEECCHHHHCHHHHEEECCCCCCE
IELTIHEGKFHQVKRMFESVGKKVVYLKRLSMGNLKLDESLELGEYRELTDEEVKLIEER
EEEEEECCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCHHHHCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose phosphate [C]

Specific reaction: uracil + D-ribose phosphate = pseudouridine 5'-phosphate + H2O [C]

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]