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The map label for this gene is ybjF [C]

Identifier: 126697975

GI number: 126697975

Start: 479068

End: 480435

Strand: Direct

Name: ybjF [C]

Synonym: CD0406

Alternate gene names: 126697975

Gene position: 479068-480435 (Clockwise)

Preceding gene: 126697974

Following gene: 126697976

Centisome position: 11.17

GC content: 33.48

Gene sequence:

>1368_bases
ATGTCTGTGAAGAAAAAAGATGTTATAGAATTTGAAGTAGATAAAATGGAATTTGGTGGTACATCTTTAAGCCAAGTTGG
AGATAGAGTTATCCATATGAAGGGTGGAATAAGTGGTCAAAAGGTGAGAGCTGGAGTTAAAAAAGTTAGAAGTAAGAAAG
CTGAAGTTAAGATGATAGAATTATTAGAGAACTCACCACTTGAAACTGAAGAAACTTGCAAGCACTTTAGAGAGTGTGGA
GGATGTACACTATTGTCTGTGCCATATGAAAAACAATTAGAAATAAAAGAAAAACAAGTTATGGATTTATTTTTAAAGCA
AGATTTATTTGGATTTCAGTTTTTAGGGATAGAAGGAAGTCCAGAAAACAAATATTATAGAAATAAGATGGAATATACTT
TTGGAGATGAAGTAAAAAATGGACCTTTGACATTAGGTCTTCATAAAAAAGGAAAGCATATAGATATACAAACTGTTGAG
GAGTGTATGTTGGTTGATGAAGACTTCTCAAAGATATTAGTTTCTAGTGTAGAGTTTTTCAATGAAAAAAAGCTTCCTTA
TTATAGAACAATGAATCACAAAGGATATTTGAGACATCTTGTAGTTAGAAAAGGGATTCACACTAATGAGATTATGGTTA
ATATAGTAACTTCTTCTCAAGAAGATTTTGATATGAATGAGTTTAAAGATATGCTTTTAGGGATTGATTTAAAAGCTGAG
TTAGTAGGTGTACTTCATACTATTAATGATGGTTTAGCAGATGCAGTCCAATGTGATGAGTTAAGAGTATTGTATGGAAG
AGATTATATACAAGAAGAAATTTTAGGTCTTAAGTTTAAGATCTCACCATTTTCGTTTTTCCAAACCAATACTAAGGGAG
CTGAGGTTCTTTATAGTATTGCTAGGGATTTTATTGGAGATTACAATGATAAAGTTGTATTTGACTTGTATAGTGGTACT
GGGTCTATTGGACAGGTTATGGCTGGTGCTGCTAAGAAAGTGTATGGTATTGAGATAGTTGAGGAAGCTGTTGTTGCTGC
TAATGAGAACGCTAAGTTGAATGGACTTACTAATTGTGAGTTTATTGCTGGGGATGTGGCTAAGGTTGTTAAGGATTTAA
AGGACAAGCCTGATTTGATTATTGTTGACCCTCCTAGACCAGGCATTCATAAGGATGCTATTAGGGATATTTGTGGATTT
GGTGCTAAGGAGATTGTTTATATTTCTTGTAATCCTAAGTCTTTGGTTGTTGATTTGGTTGATTTTAAGGGGTATGGTTA
TGAGATTAAGATGGTTAAGTGTATGGATATGTTCCCAAATACGCCACATGTGGAGTGCATAGCGTTGATACAAAGAGTGA
AATCGTGA

Upstream 100 bases:

>100_bases
ATTTAAAATTGGATGAAAGTTTAGAGTTAGGTGAGTATAGAGAGTTAACAGATGAAGAAGTAAAATTGATTGAAGAAAGA
TAATATAGTAAATTGGAGGA

Downstream 100 bases:

>100_bases
AAATCCTGCATTTTAAGCAGTTTTACAAGCATTATGTATTTGTAGAAGATGGCGAAGGTGGACGAAAAAAAGTCCTAAAA
AATTATATAGATGTGAATGT

Product: tRNA (Uracil-5-)-methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 455; Mature: 454

Protein sequence:

>455_residues
MSVKKKDVIEFEVDKMEFGGTSLSQVGDRVIHMKGGISGQKVRAGVKKVRSKKAEVKMIELLENSPLETEETCKHFRECG
GCTLLSVPYEKQLEIKEKQVMDLFLKQDLFGFQFLGIEGSPENKYYRNKMEYTFGDEVKNGPLTLGLHKKGKHIDIQTVE
ECMLVDEDFSKILVSSVEFFNEKKLPYYRTMNHKGYLRHLVVRKGIHTNEIMVNIVTSSQEDFDMNEFKDMLLGIDLKAE
LVGVLHTINDGLADAVQCDELRVLYGRDYIQEEILGLKFKISPFSFFQTNTKGAEVLYSIARDFIGDYNDKVVFDLYSGT
GSIGQVMAGAAKKVYGIEIVEEAVVAANENAKLNGLTNCEFIAGDVAKVVKDLKDKPDLIIVDPPRPGIHKDAIRDICGF
GAKEIVYISCNPKSLVVDLVDFKGYGYEIKMVKCMDMFPNTPHVECIALIQRVKS

Sequences:

>Translated_455_residues
MSVKKKDVIEFEVDKMEFGGTSLSQVGDRVIHMKGGISGQKVRAGVKKVRSKKAEVKMIELLENSPLETEETCKHFRECG
GCTLLSVPYEKQLEIKEKQVMDLFLKQDLFGFQFLGIEGSPENKYYRNKMEYTFGDEVKNGPLTLGLHKKGKHIDIQTVE
ECMLVDEDFSKILVSSVEFFNEKKLPYYRTMNHKGYLRHLVVRKGIHTNEIMVNIVTSSQEDFDMNEFKDMLLGIDLKAE
LVGVLHTINDGLADAVQCDELRVLYGRDYIQEEILGLKFKISPFSFFQTNTKGAEVLYSIARDFIGDYNDKVVFDLYSGT
GSIGQVMAGAAKKVYGIEIVEEAVVAANENAKLNGLTNCEFIAGDVAKVVKDLKDKPDLIIVDPPRPGIHKDAIRDICGF
GAKEIVYISCNPKSLVVDLVDFKGYGYEIKMVKCMDMFPNTPHVECIALIQRVKS
>Mature_454_residues
SVKKKDVIEFEVDKMEFGGTSLSQVGDRVIHMKGGISGQKVRAGVKKVRSKKAEVKMIELLENSPLETEETCKHFRECGG
CTLLSVPYEKQLEIKEKQVMDLFLKQDLFGFQFLGIEGSPENKYYRNKMEYTFGDEVKNGPLTLGLHKKGKHIDIQTVEE
CMLVDEDFSKILVSSVEFFNEKKLPYYRTMNHKGYLRHLVVRKGIHTNEIMVNIVTSSQEDFDMNEFKDMLLGIDLKAEL
VGVLHTINDGLADAVQCDELRVLYGRDYIQEEILGLKFKISPFSFFQTNTKGAEVLYSIARDFIGDYNDKVVFDLYSGTG
SIGQVMAGAAKKVYGIEIVEEAVVAANENAKLNGLTNCEFIAGDVAKVVKDLKDKPDLIIVDPPRPGIHKDAIRDICGFG
AKEIVYISCNPKSLVVDLVDFKGYGYEIKMVKCMDMFPNTPHVECIALIQRVKS

Specific function: Could Be A 23s rRNA (Uracil-5-)-Methyltransferase. [C]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI34222389, Length=416, Percent_Identity=28.3653846153846, Blast_Score=151, Evalue=1e-36,
Organism=Homo sapiens, GI51173878, Length=416, Percent_Identity=28.3653846153846, Blast_Score=151, Evalue=1e-36,
Organism=Homo sapiens, GI269784680, Length=360, Percent_Identity=29.7222222222222, Blast_Score=135, Evalue=1e-31,
Organism=Homo sapiens, GI269784676, Length=360, Percent_Identity=29.7222222222222, Blast_Score=135, Evalue=1e-31,
Organism=Homo sapiens, GI20149688, Length=360, Percent_Identity=29.7222222222222, Blast_Score=135, Evalue=1e-31,
Organism=Homo sapiens, GI269784678, Length=354, Percent_Identity=29.6610169491525, Blast_Score=134, Evalue=2e-31,
Organism=Escherichia coli, GI1787083, Length=381, Percent_Identity=27.0341207349081, Blast_Score=139, Evalue=4e-34,
Organism=Escherichia coli, GI1789148, Length=461, Percent_Identity=24.295010845987, Blast_Score=119, Evalue=6e-28,
Organism=Escherichia coli, GI1790403, Length=232, Percent_Identity=26.7241379310345, Blast_Score=66, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI71997438, Length=168, Percent_Identity=29.1666666666667, Blast_Score=76, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6322909, Length=407, Percent_Identity=24.0786240786241, Blast_Score=83, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24666579, Length=416, Percent_Identity=29.8076923076923, Blast_Score=155, Evalue=7e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016027
- InterPro:   IPR001566
- InterPro:   IPR002792
- InterPro:   IPR010280 [H]

Pfam domain/function: PF01938 TRAM; PF05958 tRNA_U5-meth_tr [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 51257; Mature: 51126

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVKKKDVIEFEVDKMEFGGTSLSQVGDRVIHMKGGISGQKVRAGVKKVRSKKAEVKMIE
CCCCCCCCEEEEEEEEECCCCCHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHH
LLENSPLETEETCKHFRECGGCTLLSVPYEKQLEIKEKQVMDLFLKQDLFGFQFLGIEGS
HHCCCCCCHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCC
PENKYYRNKMEYTFGDEVKNGPLTLGLHKKGKHIDIQTVEECMLVDEDFSKILVSSVEFF
CCCCHHHCCCCEECCCCCCCCCEEEEECCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHH
NEKKLPYYRTMNHKGYLRHLVVRKGIHTNEIMVNIVTSSQEDFDMNEFKDMLLGIDLKAE
CCCCCCCEEECCCCHHHHHHHHHCCCCCCCEEEEEEECCCCCCCHHHHHHHHHCCCHHHH
LVGVLHTINDGLADAVQCDELRVLYGRDYIQEEILGLKFKISPFSFFQTNTKGAEVLYSI
HHHHHHHHHCCHHHHHCCCCEEEECCHHHHHHHHHCEEEEECCEEEEECCCCHHHHHHHH
ARDFIGDYNDKVVFDLYSGTGSIGQVMAGAAKKVYGIEIVEEAVVAANENAKLNGLTNCE
HHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCH
FIAGDVAKVVKDLKDKPDLIIVDPPRPGIHKDAIRDICGFGAKEIVYISCNPKSLVVDLV
HHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHCCCCCEEEEEEECCCEEEEEEE
DFKGYGYEIKMVKCMDMFPNTPHVECIALIQRVKS
ECCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
SVKKKDVIEFEVDKMEFGGTSLSQVGDRVIHMKGGISGQKVRAGVKKVRSKKAEVKMIE
CCCCCCCEEEEEEEEECCCCCHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHH
LLENSPLETEETCKHFRECGGCTLLSVPYEKQLEIKEKQVMDLFLKQDLFGFQFLGIEGS
HHCCCCCCHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCC
PENKYYRNKMEYTFGDEVKNGPLTLGLHKKGKHIDIQTVEECMLVDEDFSKILVSSVEFF
CCCCHHHCCCCEECCCCCCCCCEEEEECCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHH
NEKKLPYYRTMNHKGYLRHLVVRKGIHTNEIMVNIVTSSQEDFDMNEFKDMLLGIDLKAE
CCCCCCCEEECCCCHHHHHHHHHCCCCCCCEEEEEEECCCCCCCHHHHHHHHHCCCHHHH
LVGVLHTINDGLADAVQCDELRVLYGRDYIQEEILGLKFKISPFSFFQTNTKGAEVLYSI
HHHHHHHHHCCHHHHHCCCCEEEECCHHHHHHHHHCEEEEECCEEEEECCCCHHHHHHHH
ARDFIGDYNDKVVFDLYSGTGSIGQVMAGAAKKVYGIEIVEEAVVAANENAKLNGLTNCE
HHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCH
FIAGDVAKVVKDLKDKPDLIIVDPPRPGIHKDAIRDICGFGAKEIVYISCNPKSLVVDLV
HHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHCCCCCEEEEEEECCCEEEEEEE
DFKGYGYEIKMVKCMDMFPNTPHVECIALIQRVKS
ECCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12552129 [H]