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The map label for this gene is bioB

Identifier: 126697869

GI number: 126697869

Start: 357885

End: 358862

Strand: Direct

Name: bioB

Synonym: CD0297

Alternate gene names: 126697869

Gene position: 357885-358862 (Clockwise)

Preceding gene: 126697868

Following gene: 126697870

Centisome position: 8.34

GC content: 28.73

Gene sequence:

>978_bases
ATGGAAAAATACATAATAAAATTAAAAAATAAAGTATTGAGAGAAAAAGAAATAAGTTATGAAGAAGCTCTTAATTTAAT
AAGCCTTGATACAAATAATAAAAACGATTTTGATATTCTATTAAAATCTGCAAATGAAATAAGAGAATATTTTATGGGAA
GAAAAGCAGATTTATGTACGATAATGAATGCTAAATCTGGCAAATGTAGTGAAGACTGTAAATTTTGTGCTCAGTCTTCT
TATTATAAAACTGGTGTAGAAGAATATTCATTATTAGATTACAATGAAATCTTAAATAGAGCTAAAGAGATGGAGAGTAA
AGGAGTGCATAGATTCTCATTGGTTACAAGTGGTAAAGGTATGTCAGGAAAGGAGTTTAATAATATTTTAAATATCTATG
AAGGATTGAGGGAAAATACTAATTTAAAATTATGTGCATCTTTAGGTATTATAGATTATGAGCAAGCTAAAATGTTGAAG
TCAGCAGGTGTGACTACATATCACCACAATGTTGAGACGTGTAGAGATAATTTTCATAATATATGTACAACACACACATA
TAAAGATAGGATAAAAACTATAAAAGATGCTAAAAAAGCTGGGCTAGATGTATGTGTAGGCGGTATAATTGGAATGAATG
AGAGTGAAGAACAGAGACTAAAAATGGCTTTTGAAATACGAGAATTAAATGTAAAATCATTTCCAATAAACATTTTAAAT
CCTATTAAGAATACACCAATGGAAAATTATGATGTATTAGAACCAATGGAAATACTAAAAACAACAGCAGTATTTAGGTT
TATAATTCCTAATGTGTACATAAGGTATGCTGGTGGAAGGCTATCATTAAAGGGATATGACAAAGTTGGATTTAATGGGG
GAGTAAATTCTGCAATTGTTGGTGATTATTTGACAACTGTTGGAAGTGGGATTGAAAATGATAAAAAAATGATTATAGAG
CAAGGATTTGAACTTTAA

Upstream 100 bases:

>100_bases
TTGTGTCTAAAATAAAGTTGACCAATAAATGTTATATATGCAATACTATATATAATAAGTAAACTACATAAAAATATTAA
GTTGACAAATAGGAGGATAT

Downstream 100 bases:

>100_bases
TAAATACTGCTTAAATTAATTAATATACTTTTAGATAATTATAAAAGTATATTAATTTTTTTTTGCAGATTAATTTAAAT
ATTAAAATTAAATTATAAGT

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 325; Mature: 325

Protein sequence:

>325_residues
MEKYIIKLKNKVLREKEISYEEALNLISLDTNNKNDFDILLKSANEIREYFMGRKADLCTIMNAKSGKCSEDCKFCAQSS
YYKTGVEEYSLLDYNEILNRAKEMESKGVHRFSLVTSGKGMSGKEFNNILNIYEGLRENTNLKLCASLGIIDYEQAKMLK
SAGVTTYHHNVETCRDNFHNICTTHTYKDRIKTIKDAKKAGLDVCVGGIIGMNESEEQRLKMAFEIRELNVKSFPINILN
PIKNTPMENYDVLEPMEILKTTAVFRFIIPNVYIRYAGGRLSLKGYDKVGFNGGVNSAIVGDYLTTVGSGIENDKKMIIE
QGFEL

Sequences:

>Translated_325_residues
MEKYIIKLKNKVLREKEISYEEALNLISLDTNNKNDFDILLKSANEIREYFMGRKADLCTIMNAKSGKCSEDCKFCAQSS
YYKTGVEEYSLLDYNEILNRAKEMESKGVHRFSLVTSGKGMSGKEFNNILNIYEGLRENTNLKLCASLGIIDYEQAKMLK
SAGVTTYHHNVETCRDNFHNICTTHTYKDRIKTIKDAKKAGLDVCVGGIIGMNESEEQRLKMAFEIRELNVKSFPINILN
PIKNTPMENYDVLEPMEILKTTAVFRFIIPNVYIRYAGGRLSLKGYDKVGFNGGVNSAIVGDYLTTVGSGIENDKKMIIE
QGFEL
>Mature_325_residues
MEKYIIKLKNKVLREKEISYEEALNLISLDTNNKNDFDILLKSANEIREYFMGRKADLCTIMNAKSGKCSEDCKFCAQSS
YYKTGVEEYSLLDYNEILNRAKEMESKGVHRFSLVTSGKGMSGKEFNNILNIYEGLRENTNLKLCASLGIIDYEQAKMLK
SAGVTTYHHNVETCRDNFHNICTTHTYKDRIKTIKDAKKAGLDVCVGGIIGMNESEEQRLKMAFEIRELNVKSFPINILN
PIKNTPMENYDVLEPMEILKTTAVFRFIIPNVYIRYAGGRLSLKGYDKVGFNGGVNSAIVGDYLTTVGSGIENDKKMIIE
QGFEL

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family

Homologues:

Organism=Escherichia coli, GI1786992, Length=284, Percent_Identity=31.6901408450704, Blast_Score=176, Evalue=2e-45,
Organism=Saccharomyces cerevisiae, GI6321725, Length=290, Percent_Identity=37.2413793103448, Blast_Score=201, Evalue=2e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOB_CLOD6 (Q18D35)

Other databases:

- EMBL:   AM180355
- RefSeq:   YP_001086766.1
- ProteinModelPortal:   Q18D35
- SMR:   Q18D35
- STRING:   Q18D35
- GeneID:   4913622
- GenomeReviews:   AM180355_GR
- KEGG:   cdf:CD0297
- NMPDR:   fig|1496.1.peg.1359
- eggNOG:   COG0502
- HOGENOM:   HBG405569
- OMA:   MGMETCM
- ProtClustDB:   CLSK734793
- HAMAP:   MF_01694
- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00876
- SMART:   SM00729
- TIGRFAMs:   TIGR00433

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM

EC number: =2.8.1.6

Molecular weight: Translated: 36876; Mature: 36876

Theoretical pI: Translated: 7.70; Mature: 7.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKYIIKLKNKVLREKEISYEEALNLISLDTNNKNDFDILLKSANEIREYFMGRKADLCT
CCCHHHHHHHHHHHHHHCCHHHHCCEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCEEE
IMNAKSGKCSEDCKFCAQSSYYKTGVEEYSLLDYNEILNRAKEMESKGVHRFSLVTSGKG
EECCCCCCCHHHHHHHHCCCHHHCCCHHHHHCCHHHHHHHHHHHHHCCCEEEEEEECCCC
MSGKEFNNILNIYEGLRENTNLKLCASLGIIDYEQAKMLKSAGVTTYHHNVETCRDNFHN
CCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHCCCEEEECCHHHHHHHHHH
ICTTHTYKDRIKTIKDAKKAGLDVCVGGIIGMNESEEQRLKMAFEIRELNVKSFPINILN
HHCCCHHHHHHHHHHHHHHCCHHEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHC
PIKNTPMENYDVLEPMEILKTTAVFRFIIPNVYIRYAGGRLSLKGYDKVGFNGGVNSAIV
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEECCCEEEECCCCCCCCCCCCCHHHH
GDYLTTVGSGIENDKKMIIEQGFEL
HHHHHHHCCCCCCHHHHHHHCCCCC
>Mature Secondary Structure
MEKYIIKLKNKVLREKEISYEEALNLISLDTNNKNDFDILLKSANEIREYFMGRKADLCT
CCCHHHHHHHHHHHHHHCCHHHHCCEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCEEE
IMNAKSGKCSEDCKFCAQSSYYKTGVEEYSLLDYNEILNRAKEMESKGVHRFSLVTSGKG
EECCCCCCCHHHHHHHHCCCHHHCCCHHHHHCCHHHHHHHHHHHHHCCCEEEEEEECCCC
MSGKEFNNILNIYEGLRENTNLKLCASLGIIDYEQAKMLKSAGVTTYHHNVETCRDNFHN
CCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHCCCEEEECCHHHHHHHHHH
ICTTHTYKDRIKTIKDAKKAGLDVCVGGIIGMNESEEQRLKMAFEIRELNVKSFPINILN
HHCCCHHHHHHHHHHHHHHCCHHEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHC
PIKNTPMENYDVLEPMEILKTTAVFRFIIPNVYIRYAGGRLSLKGYDKVGFNGGVNSAIV
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEECCCEEEECCCCCCCCCCCCCHHHH
GDYLTTVGSGIENDKKMIIEQGFEL
HHHHHHHCCCCCCHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA