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The map label for this gene is ydeM [C]

Identifier: 126697732

GI number: 126697732

Start: 213854

End: 215275

Strand: Direct

Name: ydeM [C]

Synonym: CD0162

Alternate gene names: 126697732

Gene position: 213854-215275 (Clockwise)

Preceding gene: 126697731

Following gene: 126697733

Centisome position: 4.98

GC content: 26.23

Gene sequence:

>1422_bases
ATGAATCAAAAACCATTTATACACACATTTAAAGCTGGAAAATCATATTTTATTTATGATGTTAATACGGATAAGATTTT
AAAGGTAAATGCTGCTGTTTATAATTATCTAAATAAAATTGAGCATAATTTAGAAAAAGAAAGTGACTGGAACATAGAAA
TAGAAGACGAAATTAATACCTTGATTAATTATGGATTTCTAAAAAATAAAAGGGTAAGTAAAACAATGCACCCTGAAACT
AAGTATATAAAATATCATATAGAAAATAATCTATCCTCTATTATTTTACAAGTAACCCAAAATTGTAATTTACGTTGTGA
CTATTGCATTTATTCTGGTTCATACAGCAACAGAGTTCATACAAATAAAAGAATGAATATAGATGTAGCAAAAAGAAGTA
TTGACTATTTGATTAAGAATTCTAGTCAATTAAATGAAGTTAGTGTTGGCTTTTATGGAGGAGAACCCTTACTTGAGTTT
GATTTGATTAAGCAGATAGTAGAATATGTAAAAGTAGTTGGAGAAGGAAAGGAAATATACTTTAATCTAACTACAAATGC
AACTTTATTTAATGAGGAAATAATAGAATTCTTTGTAAAAAATAATATAACAACTATGATTAGTTTAGATGGAGGAAAAG
AAATTCATGATAAAAGTAGAAAATTTGCAAATACAGGAGAAGGATCTCATGATGTTGTATTAAAAAATGTACGTTACATA
AAAAATAAATATCCAGAATATTATAAAAAGAATATTACATTTAATACAGTGATAAATTCAGAAAATGATTACATTTCTAT
AGATGATTTTATATCTAAAAATGAGACTTTAAAAGATTCAAACTTTTTAGCTTCCACTCTTAATAATAACTATTCTGATA
AAGAAATAGTTTCATCAGATGAATATATATTAGGTAAGAGTTATGATAGATTCTTGACTTTACTCAACAAATTAAAATTT
ATAAAAGATGAGAGTATGTCACCTTTATCAAGAATAGAAAGAGCCTTATTAGATGATTTTAGATATGAGAAAAATATTGA
AGGTAGATTAGAGTTACCAGATGTGTGGCATCATGGTGGACCATGTATACCAGGAGCTTTTAGAATATTTGTAACTACAG
ATGGAGAATTTTTTCCATGTGAGAGAGTAAGTGAAAGTTCTAAAATGGGTATGATTGGAAATTTAGATAGTGGAGTAGAT
ATAGAAAAGGCTGAAAAACTTTTAAATATAGGAAAATTATATGAAGAGCAATGTTCTGATTGTTGGGCCTATAGTTATTG
TGATATATGTATAGCTAAGATTGATGATACTAAATGTAATGAAAATGAAATAAAACCACATGATTGTGACAGAATTAGAC
ATTCTACAGAAAATAATATGAAAGATTACTGCGTTTTGAAATCTCTAGGATATCAATATTAA

Upstream 100 bases:

>100_bases
GTTGTTAGAAAGTGGAAGACATGATGAACTTATGGACAAAAAGGGTGTTTATTTTAATTTATTTTGTAACTGCAAATAAA
TATTAAAGGGAGGTTAATTA

Downstream 100 bases:

>100_bases
ATTTTCTAATGAAAGGAAAGATGGGATTATGGAAAAAGTACTTATATATCCATTTAATTATAATTTTTTTACTATTTTAA
ATCATTGCAATAAAATTTCA

Product: radical SAM family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 473; Mature: 473

Protein sequence:

>473_residues
MNQKPFIHTFKAGKSYFIYDVNTDKILKVNAAVYNYLNKIEHNLEKESDWNIEIEDEINTLINYGFLKNKRVSKTMHPET
KYIKYHIENNLSSIILQVTQNCNLRCDYCIYSGSYSNRVHTNKRMNIDVAKRSIDYLIKNSSQLNEVSVGFYGGEPLLEF
DLIKQIVEYVKVVGEGKEIYFNLTTNATLFNEEIIEFFVKNNITTMISLDGGKEIHDKSRKFANTGEGSHDVVLKNVRYI
KNKYPEYYKKNITFNTVINSENDYISIDDFISKNETLKDSNFLASTLNNNYSDKEIVSSDEYILGKSYDRFLTLLNKLKF
IKDESMSPLSRIERALLDDFRYEKNIEGRLELPDVWHHGGPCIPGAFRIFVTTDGEFFPCERVSESSKMGMIGNLDSGVD
IEKAEKLLNIGKLYEEQCSDCWAYSYCDICIAKIDDTKCNENEIKPHDCDRIRHSTENNMKDYCVLKSLGYQY

Sequences:

>Translated_473_residues
MNQKPFIHTFKAGKSYFIYDVNTDKILKVNAAVYNYLNKIEHNLEKESDWNIEIEDEINTLINYGFLKNKRVSKTMHPET
KYIKYHIENNLSSIILQVTQNCNLRCDYCIYSGSYSNRVHTNKRMNIDVAKRSIDYLIKNSSQLNEVSVGFYGGEPLLEF
DLIKQIVEYVKVVGEGKEIYFNLTTNATLFNEEIIEFFVKNNITTMISLDGGKEIHDKSRKFANTGEGSHDVVLKNVRYI
KNKYPEYYKKNITFNTVINSENDYISIDDFISKNETLKDSNFLASTLNNNYSDKEIVSSDEYILGKSYDRFLTLLNKLKF
IKDESMSPLSRIERALLDDFRYEKNIEGRLELPDVWHHGGPCIPGAFRIFVTTDGEFFPCERVSESSKMGMIGNLDSGVD
IEKAEKLLNIGKLYEEQCSDCWAYSYCDICIAKIDDTKCNENEIKPHDCDRIRHSTENNMKDYCVLKSLGYQY
>Mature_473_residues
MNQKPFIHTFKAGKSYFIYDVNTDKILKVNAAVYNYLNKIEHNLEKESDWNIEIEDEINTLINYGFLKNKRVSKTMHPET
KYIKYHIENNLSSIILQVTQNCNLRCDYCIYSGSYSNRVHTNKRMNIDVAKRSIDYLIKNSSQLNEVSVGFYGGEPLLEF
DLIKQIVEYVKVVGEGKEIYFNLTTNATLFNEEIIEFFVKNNITTMISLDGGKEIHDKSRKFANTGEGSHDVVLKNVRYI
KNKYPEYYKKNITFNTVINSENDYISIDDFISKNETLKDSNFLASTLNNNYSDKEIVSSDEYILGKSYDRFLTLLNKLKF
IKDESMSPLSRIERALLDDFRYEKNIEGRLELPDVWHHGGPCIPGAFRIFVTTDGEFFPCERVSESSKMGMIGNLDSGVD
IEKAEKLLNIGKLYEEQCSDCWAYSYCDICIAKIDDTKCNENEIKPHDCDRIRHSTENNMKDYCVLKSLGYQY

Specific function: Unknown

COG id: COG0641

COG function: function code R; Arylsulfatase regulator (Fe-S oxidoreductase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Anaerobic sulfatase-maturating enzyme family [H]

Homologues:

Organism=Escherichia coli, GI87081923, Length=168, Percent_Identity=31.547619047619, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI48994973, Length=173, Percent_Identity=27.7456647398844, Blast_Score=75, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR000385
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 54924; Mature: 54924

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: PS01305 MOAA_NIFB_PQQE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQKPFIHTFKAGKSYFIYDVNTDKILKVNAAVYNYLNKIEHNLEKESDWNIEIEDEINT
CCCCCCEEEEECCCEEEEEECCCCCEEEEHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHH
LINYGFLKNKRVSKTMHPETKYIKYHIENNLSSIILQVTQNCNLRCDYCIYSGSYSNRVH
HHHHCCCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHCCCCCEEEEEEEECCCCCCEE
TNKRMNIDVAKRSIDYLIKNSSQLNEVSVGFYGGEPLLEFDLIKQIVEYVKVVGEGKEIY
CCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCEEE
FNLTTNATLFNEEIIEFFVKNNITTMISLDGGKEIHDKSRKFANTGEGSHDVVLKNVRYI
EEEECCCEEHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHCCCCCCCCHHHHHHHHHH
KNKYPEYYKKNITFNTVINSENDYISIDDFISKNETLKDSNFLASTLNNNYSDKEIVSSD
HHCCHHHHHCCCEEEEEECCCCCEEEHHHHHCCCCCCCCCCHHHHHHCCCCCCHHHCCCC
EYILGKSYDRFLTLLNKLKFIKDESMSPLSRIERALLDDFRYEKNIEGRLELPDVWHHGG
CEEECCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCEEECCHHHCCCC
PCIPGAFRIFVTTDGEFFPCERVSESSKMGMIGNLDSGVDIEKAEKLLNIGKLYEEQCSD
CCCCCEEEEEEEECCCCCCHHHCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHH
CWAYSYCDICIAKIDDTKCNENEIKPHDCDRIRHSTENNMKDYCVLKSLGYQY
HHHHHHHEEEEEECCCCCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHCCCCC
>Mature Secondary Structure
MNQKPFIHTFKAGKSYFIYDVNTDKILKVNAAVYNYLNKIEHNLEKESDWNIEIEDEINT
CCCCCCEEEEECCCEEEEEECCCCCEEEEHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHH
LINYGFLKNKRVSKTMHPETKYIKYHIENNLSSIILQVTQNCNLRCDYCIYSGSYSNRVH
HHHHCCCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHCCCCCEEEEEEEECCCCCCEE
TNKRMNIDVAKRSIDYLIKNSSQLNEVSVGFYGGEPLLEFDLIKQIVEYVKVVGEGKEIY
CCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCEEE
FNLTTNATLFNEEIIEFFVKNNITTMISLDGGKEIHDKSRKFANTGEGSHDVVLKNVRYI
EEEECCCEEHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHCCCCCCCCHHHHHHHHHH
KNKYPEYYKKNITFNTVINSENDYISIDDFISKNETLKDSNFLASTLNNNYSDKEIVSSD
HHCCHHHHHCCCEEEEEECCCCCEEEHHHHHCCCCCCCCCCHHHHHHCCCCCCHHHCCCC
EYILGKSYDRFLTLLNKLKFIKDESMSPLSRIERALLDDFRYEKNIEGRLELPDVWHHGG
CEEECCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCEEECCHHHCCCC
PCIPGAFRIFVTTDGEFFPCERVSESSKMGMIGNLDSGVDIEKAEKLLNIGKLYEEQCSD
CCCCCEEEEEEEECCCCCCHHHCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHH
CWAYSYCDICIAKIDDTKCNENEIKPHDCDRIRHSTENNMKDYCVLKSLGYQY
HHHHHHHEEEEEECCCCCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]