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The map label for this gene is truA1 [H]

Identifier: 126697674

GI number: 126697674

Start: 120957

End: 121688

Strand: Direct

Name: truA1 [H]

Synonym: CD0103

Alternate gene names: 126697674

Gene position: 120957-121688 (Clockwise)

Preceding gene: 126697673

Following gene: 126697675

Centisome position: 2.82

GC content: 29.64

Gene sequence:

>732_bases
ATGAGAAATATAAAAATTAAAATCCAATACAATGGTAAGAATTATTGTGGATGGCAAAAGCAACCTGATTCTTTAGGGAT
ACAAGGTACAATTGAAAGGGCTATATATGATATAACAAAAGAAGAAACCAGTCTTATAGGGTCTGGTAGAACTGATTCAG
GAGTTCATGCTATTGGTCAGATTGCAAATTTTAAAATTAATTCAGGTATAAGTATAGAAAGTATACCAATGGCACTAAAT
GCCAAGTTGCCAAAAGACATATCAGTTATAGAAGCATGTGAAGTAAATGATGATTTTCACTCCAGATACAGTGCAAAAGG
TAAAACATATAAATATTTAGTATATAATAGCAAGTTTAGAAATCCAATACTTAGTGAGATATCATATCAAGTAAAATATG
AACTTGATTTTGATAAAATGTGTTCAGAAGCAAAAAGTTTATTAGGAACACATGATTTTAAAGGTTTTATGAGTTCTGGT
TCATCTGTAAAAGATACAGTTAGAACGATATATGATATTGATATAAGTAAAAAAGATGACTTGATTACTTTTGAGATTAG
TGGTAATGGTTTTTTATATAACATGGTGAGGATTATTGTTGGAACACTTGTTGATATGGGTCGTGGTAGAATTAACGAAC
CATTTTTAGATATAATACAATCAAAAACTAGGTCAAGATGTGGACATACAGCTCCAGCACAAGGATTATTTCTCAAAAAA
GTTCATTATTAA

Upstream 100 bases:

>100_bases
TATATGGCATGTGTATTTCAGCTTGTTTATCTGGGAGCAATTATAGCTACTAGATTTATAGCAATTTAATTAATAAGTTA
TAGATATAATGAGGTAAAGT

Downstream 100 bases:

>100_bases
CTTGACACACCAGGCTACATGTATTAAAATATATGAGAATGTGTTAATAAGTCCACTTGCCCCGGACTTGACATAAACGG
TAAAAAAATTTTATGAAAAG

Product: tRNA pseudouridine synthase 1

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I [H]

Number of amino acids: Translated: 243; Mature: 243

Protein sequence:

>243_residues
MRNIKIKIQYNGKNYCGWQKQPDSLGIQGTIERAIYDITKEETSLIGSGRTDSGVHAIGQIANFKINSGISIESIPMALN
AKLPKDISVIEACEVNDDFHSRYSAKGKTYKYLVYNSKFRNPILSEISYQVKYELDFDKMCSEAKSLLGTHDFKGFMSSG
SSVKDTVRTIYDIDISKKDDLITFEISGNGFLYNMVRIIVGTLVDMGRGRINEPFLDIIQSKTRSRCGHTAPAQGLFLKK
VHY

Sequences:

>Translated_243_residues
MRNIKIKIQYNGKNYCGWQKQPDSLGIQGTIERAIYDITKEETSLIGSGRTDSGVHAIGQIANFKINSGISIESIPMALN
AKLPKDISVIEACEVNDDFHSRYSAKGKTYKYLVYNSKFRNPILSEISYQVKYELDFDKMCSEAKSLLGTHDFKGFMSSG
SSVKDTVRTIYDIDISKKDDLITFEISGNGFLYNMVRIIVGTLVDMGRGRINEPFLDIIQSKTRSRCGHTAPAQGLFLKK
VHY
>Mature_243_residues
MRNIKIKIQYNGKNYCGWQKQPDSLGIQGTIERAIYDITKEETSLIGSGRTDSGVHAIGQIANFKINSGISIESIPMALN
AKLPKDISVIEACEVNDDFHSRYSAKGKTYKYLVYNSKFRNPILSEISYQVKYELDFDKMCSEAKSLLGTHDFKGFMSSG
SSVKDTVRTIYDIDISKKDDLITFEISGNGFLYNMVRIIVGTLVDMGRGRINEPFLDIIQSKTRSRCGHTAPAQGLFLKK
VHY

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs [H]

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family [H]

Homologues:

Organism=Homo sapiens, GI23503259, Length=269, Percent_Identity=30.8550185873606, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI199559785, Length=251, Percent_Identity=26.2948207171315, Blast_Score=72, Evalue=4e-13,
Organism=Escherichia coli, GI1788657, Length=241, Percent_Identity=32.7800829875519, Blast_Score=164, Evalue=6e-42,
Organism=Caenorhabditis elegans, GI71985046, Length=253, Percent_Identity=28.0632411067194, Blast_Score=77, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6321112, Length=233, Percent_Identity=26.1802575107296, Blast_Score=66, Evalue=6e-12,
Organism=Drosophila melanogaster, GI116007752, Length=305, Percent_Identity=29.5081967213115, Blast_Score=87, Evalue=9e-18,
Organism=Drosophila melanogaster, GI281362146, Length=269, Percent_Identity=25.6505576208178, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24648455, Length=269, Percent_Identity=25.6505576208178, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094 [H]

Pfam domain/function: PF01416 PseudoU_synth_1 [H]

EC number: =5.4.99.12 [H]

Molecular weight: Translated: 27321; Mature: 27321

Theoretical pI: Translated: 9.20; Mature: 9.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNIKIKIQYNGKNYCGWQKQPDSLGIQGTIERAIYDITKEETSLIGSGRTDSGVHAIGQ
CCCEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHH
IANFKINSGISIESIPMALNAKLPKDISVIEACEVNDDFHSRYSAKGKTYKYLVYNSKFR
HHCEEECCCCCEEECCEEECCCCCCCCCEEEEECCCCHHHHHHCCCCCEEEEEEECCCCC
NPILSEISYQVKYELDFDKMCSEAKSLLGTHDFKGFMSSGSSVKDTVRTIYDIDISKKDD
CHHHHHCEEEEEEEECHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHCCCCCCCCC
LITFEISGNGFLYNMVRIIVGTLVDMGRGRINEPFLDIIQSKTRSRCGHTAPAQGLFLKK
EEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEE
VHY
CCC
>Mature Secondary Structure
MRNIKIKIQYNGKNYCGWQKQPDSLGIQGTIERAIYDITKEETSLIGSGRTDSGVHAIGQ
CCCEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHH
IANFKINSGISIESIPMALNAKLPKDISVIEACEVNDDFHSRYSAKGKTYKYLVYNSKFR
HHCEEECCCCCEEECCEEECCCCCCCCCEEEEECCCCHHHHHHCCCCCEEEEEEECCCCC
NPILSEISYQVKYELDFDKMCSEAKSLLGTHDFKGFMSSGSSVKDTVRTIYDIDISKKDD
CHHHHHCEEEEEEEECHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHCCCCCCCCC
LITFEISGNGFLYNMVRIIVGTLVDMGRGRINEPFLDIIQSKTRSRCGHTAPAQGLFLKK
EEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEE
VHY
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA