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The map label for this gene is tufA

Identifier: 126697624

GI number: 126697624

Start: 83724

End: 84917

Strand: Direct

Name: tufA

Synonym: CD0058

Alternate gene names: 126697624

Gene position: 83724-84917 (Clockwise)

Preceding gene: 126697623

Following gene: 126697625

Centisome position: 1.95

GC content: 37.27

Gene sequence:

>1194_bases
ATGGCTAAAGCTAAATACGAAAGAACAAAACCTCATGTTAATATAGGGACAATAGGACACGTAGACCACGGTAAAACTAC
ATTAACAGCAGCAATAACAAAAACATTATATGACAGATATCAATTAGGAGAAGCAGTAGATTTCGCAAACATAGATAAAG
CTCCAGAAGAAAGAGAAAGAGGAATCACAATATCAACAGCACACGTTGAGTATGAAACACCAAATAGACACTATGCACAC
GTTGACTGCCCAGGACATGCTGACTACGTTAAGAACATGATAACAGGAGCAGCACAAATGGACGGAGCAATATTAGTTTG
TTCAGCAACAGATGGACCAATGCCACAAACAAGAGAGCATATACTATTATCAAGACAAGTTGGAGTACCATATATAGTAG
TATTCTTAAACAAATGTGACATGGTAGATGATGAAGAGTTATTAGAGTTAGTAGAGATGGAAGTAAGAGATTTATTAACA
GAATATGATTTCCCAGGAGATGACACTCCAATAGTAAGAGGTTCAGCATTAATGGCATTAGAAGATCCAAAGAGCGAGTG
GGGAGATAAGATAGTAGAATTATTCGAGCAAATAGATGAGTATATACCAGCTCCAGAGAGAGATACAGATAAACCATTCT
TAATGCCAGTAGAGGACGTATTCTCAATCACAGGAAGAGGAACAGTTGCAACAGGAAGAGTGGAAAGAGGAGTACTAAAA
GTACAAGACGAAGTAGAATTAGTAGGATTAACAGAAGCACCAAGAAAAGTAGTAGTAACAGGAGTAGAGATGTTCAGAAA
ATTATTAGACCAAGCACAAGCAGGGGATAATATAGGAGCATTATTAAGAGGAGTACAAAGAAACGAGATAGAAAGAGGAC
AAGTACTAGCAAAGACTGGATCAGTAAAGGCACACACAAAGTTTACAGCAGAAGTATATGTACTTAAAAAAGAAGAGGGT
GGAAGACATACACCATTCTTTGATGGATATAGACCACAATTCTATTTCAGAACAACAGACGTAACAGGAGCTTGTAAGTT
ACCAGAAGGAATAGAGATGGTAATGCCTGGAGATAACGTAACAATGGAAGTAGACTTAATAAACTCAATAGTTGTAGAAG
AGGGATTAAGATTCTCAATAAGAGAAGGTGGAAGAACAGTAGCTTCAGGAGTTGTTGCTACAATAATAGAGTAA

Upstream 100 bases:

>100_bases
AAACATCTTGAAAACAGAAATGATTAATAGTAAAATACTTACCATAAGCTTATTGTGAGCAAAATTAATTTATATCGAAA
GATAAGGGAGGAAATTCAAA

Downstream 100 bases:

>100_bases
TTCTTTACTAAACTGATGGATTAAAAGGAATAACAAAAAGAGGGGTTATGCTCCTCTTTTTTTAACATAGAAATACAAAA
ACATTGACACAGCATAGGAT

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu

Number of amino acids: Translated: 397; Mature: 396

Protein sequence:

>397_residues
MAKAKYERTKPHVNIGTIGHVDHGKTTLTAAITKTLYDRYQLGEAVDFANIDKAPEERERGITISTAHVEYETPNRHYAH
VDCPGHADYVKNMITGAAQMDGAILVCSATDGPMPQTREHILLSRQVGVPYIVVFLNKCDMVDDEELLELVEMEVRDLLT
EYDFPGDDTPIVRGSALMALEDPKSEWGDKIVELFEQIDEYIPAPERDTDKPFLMPVEDVFSITGRGTVATGRVERGVLK
VQDEVELVGLTEAPRKVVVTGVEMFRKLLDQAQAGDNIGALLRGVQRNEIERGQVLAKTGSVKAHTKFTAEVYVLKKEEG
GRHTPFFDGYRPQFYFRTTDVTGACKLPEGIEMVMPGDNVTMEVDLINSIVVEEGLRFSIREGGRTVASGVVATIIE

Sequences:

>Translated_397_residues
MAKAKYERTKPHVNIGTIGHVDHGKTTLTAAITKTLYDRYQLGEAVDFANIDKAPEERERGITISTAHVEYETPNRHYAH
VDCPGHADYVKNMITGAAQMDGAILVCSATDGPMPQTREHILLSRQVGVPYIVVFLNKCDMVDDEELLELVEMEVRDLLT
EYDFPGDDTPIVRGSALMALEDPKSEWGDKIVELFEQIDEYIPAPERDTDKPFLMPVEDVFSITGRGTVATGRVERGVLK
VQDEVELVGLTEAPRKVVVTGVEMFRKLLDQAQAGDNIGALLRGVQRNEIERGQVLAKTGSVKAHTKFTAEVYVLKKEEG
GRHTPFFDGYRPQFYFRTTDVTGACKLPEGIEMVMPGDNVTMEVDLINSIVVEEGLRFSIREGGRTVASGVVATIIE
>Mature_396_residues
AKAKYERTKPHVNIGTIGHVDHGKTTLTAAITKTLYDRYQLGEAVDFANIDKAPEERERGITISTAHVEYETPNRHYAHV
DCPGHADYVKNMITGAAQMDGAILVCSATDGPMPQTREHILLSRQVGVPYIVVFLNKCDMVDDEELLELVEMEVRDLLTE
YDFPGDDTPIVRGSALMALEDPKSEWGDKIVELFEQIDEYIPAPERDTDKPFLMPVEDVFSITGRGTVATGRVERGVLKV
QDEVELVGLTEAPRKVVVTGVEMFRKLLDQAQAGDNIGALLRGVQRNEIERGQVLAKTGSVKAHTKFTAEVYVLKKEEGG
RHTPFFDGYRPQFYFRTTDVTGACKLPEGIEMVMPGDNVTMEVDLINSIVVEEGLRFSIREGGRTVASGVVATIIE

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily

Homologues:

Organism=Homo sapiens, GI34147630, Length=399, Percent_Identity=55.3884711779449, Blast_Score=449, Evalue=1e-126,
Organism=Homo sapiens, GI4503475, Length=446, Percent_Identity=30.7174887892377, Blast_Score=162, Evalue=5e-40,
Organism=Homo sapiens, GI4503471, Length=456, Percent_Identity=30.4824561403509, Blast_Score=158, Evalue=9e-39,
Organism=Homo sapiens, GI223555963, Length=444, Percent_Identity=30.6306306306306, Blast_Score=156, Evalue=3e-38,
Organism=Homo sapiens, GI5729864, Length=444, Percent_Identity=30.6306306306306, Blast_Score=156, Evalue=4e-38,
Organism=Homo sapiens, GI54607086, Length=349, Percent_Identity=30.3724928366762, Blast_Score=129, Evalue=7e-30,
Organism=Homo sapiens, GI194018522, Length=339, Percent_Identity=29.2035398230088, Blast_Score=108, Evalue=9e-24,
Organism=Homo sapiens, GI194097354, Length=339, Percent_Identity=29.2035398230088, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI194018520, Length=339, Percent_Identity=29.2035398230088, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI46094014, Length=336, Percent_Identity=29.4642857142857, Blast_Score=106, Evalue=5e-23,
Organism=Homo sapiens, GI4503507, Length=289, Percent_Identity=28.719723183391, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI157426893, Length=243, Percent_Identity=27.9835390946502, Blast_Score=72, Evalue=9e-13,
Organism=Homo sapiens, GI53729339, Length=231, Percent_Identity=29.4372294372294, Blast_Score=70, Evalue=4e-12,
Organism=Homo sapiens, GI53729337, Length=231, Percent_Identity=29.4372294372294, Blast_Score=70, Evalue=4e-12,
Organism=Escherichia coli, GI1790412, Length=396, Percent_Identity=74.2424242424242, Blast_Score=579, Evalue=1e-166,
Organism=Escherichia coli, GI1789737, Length=396, Percent_Identity=74.2424242424242, Blast_Score=579, Evalue=1e-166,
Organism=Escherichia coli, GI2367247, Length=340, Percent_Identity=30, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI48994988, Length=273, Percent_Identity=32.967032967033, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1789108, Length=264, Percent_Identity=33.3333333333333, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1788922, Length=335, Percent_Identity=27.1641791044776, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1789738, Length=146, Percent_Identity=34.9315068493151, Blast_Score=65, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17556456, Length=401, Percent_Identity=50.8728179551122, Blast_Score=385, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI25141371, Length=388, Percent_Identity=40.979381443299, Blast_Score=314, Evalue=5e-86,
Organism=Caenorhabditis elegans, GI17552884, Length=452, Percent_Identity=30.7522123893805, Blast_Score=160, Evalue=8e-40,
Organism=Caenorhabditis elegans, GI17569207, Length=452, Percent_Identity=30.7522123893805, Blast_Score=160, Evalue=8e-40,
Organism=Caenorhabditis elegans, GI32566303, Length=438, Percent_Identity=29.9086757990868, Blast_Score=139, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI115532067, Length=432, Percent_Identity=27.5462962962963, Blast_Score=128, Evalue=7e-30,
Organism=Caenorhabditis elegans, GI115532065, Length=432, Percent_Identity=27.5462962962963, Blast_Score=127, Evalue=8e-30,
Organism=Caenorhabditis elegans, GI32566629, Length=358, Percent_Identity=31.5642458100559, Blast_Score=125, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI17506081, Length=275, Percent_Identity=31.2727272727273, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI32566301, Length=148, Percent_Identity=38.5135135135135, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17509919, Length=282, Percent_Identity=25.177304964539, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17557151, Length=237, Percent_Identity=26.5822784810127, Blast_Score=72, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6324761, Length=399, Percent_Identity=65.6641604010025, Blast_Score=535, Evalue=1e-153,
Organism=Saccharomyces cerevisiae, GI6325337, Length=454, Percent_Identity=31.7180616740088, Blast_Score=168, Evalue=1e-42,
Organism=Saccharomyces cerevisiae, GI6319594, Length=454, Percent_Identity=31.7180616740088, Blast_Score=168, Evalue=1e-42,
Organism=Saccharomyces cerevisiae, GI6320377, Length=344, Percent_Identity=29.3604651162791, Blast_Score=95, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6322937, Length=366, Percent_Identity=26.775956284153, Blast_Score=94, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6320863, Length=282, Percent_Identity=27.3049645390071, Blast_Score=85, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6323320, Length=242, Percent_Identity=27.6859504132231, Blast_Score=67, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6323098, Length=135, Percent_Identity=35.5555555555556, Blast_Score=66, Evalue=1e-11,
Organism=Drosophila melanogaster, GI281363316, Length=395, Percent_Identity=57.2151898734177, Blast_Score=462, Evalue=1e-130,
Organism=Drosophila melanogaster, GI17864358, Length=395, Percent_Identity=57.2151898734177, Blast_Score=462, Evalue=1e-130,
Organism=Drosophila melanogaster, GI19921738, Length=386, Percent_Identity=48.7046632124352, Blast_Score=393, Evalue=1e-109,
Organism=Drosophila melanogaster, GI45553807, Length=455, Percent_Identity=30.3296703296703, Blast_Score=162, Evalue=3e-40,
Organism=Drosophila melanogaster, GI45553816, Length=455, Percent_Identity=30.3296703296703, Blast_Score=162, Evalue=3e-40,
Organism=Drosophila melanogaster, GI24651721, Length=455, Percent_Identity=30.3296703296703, Blast_Score=162, Evalue=3e-40,
Organism=Drosophila melanogaster, GI17864154, Length=455, Percent_Identity=30.3296703296703, Blast_Score=162, Evalue=3e-40,
Organism=Drosophila melanogaster, GI24652838, Length=462, Percent_Identity=31.6017316017316, Blast_Score=156, Evalue=3e-38,
Organism=Drosophila melanogaster, GI17137572, Length=462, Percent_Identity=31.6017316017316, Blast_Score=156, Evalue=3e-38,
Organism=Drosophila melanogaster, GI45550900, Length=454, Percent_Identity=29.295154185022, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI19922690, Length=305, Percent_Identity=32.4590163934426, Blast_Score=129, Evalue=3e-30,
Organism=Drosophila melanogaster, GI17137380, Length=342, Percent_Identity=30.1169590643275, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI24647046, Length=281, Percent_Identity=29.1814946619217, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24647048, Length=281, Percent_Identity=29.1814946619217, Blast_Score=89, Evalue=4e-18,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): EFTU_CLOD6 (Q18CE4)

Other databases:

- EMBL:   AM180355
- EMBL:   AM180355
- RefSeq:   YP_001086521.1
- RefSeq:   YP_001086535.1
- ProteinModelPortal:   Q18CE4
- SMR:   Q18CE4
- STRING:   Q18CE4
- GeneID:   4914221
- GeneID:   4916670
- GenomeReviews:   AM180355_GR
- GenomeReviews:   AM180355_GR
- KEGG:   cdf:CD0058
- KEGG:   cdf:CD0071
- eggNOG:   COG0050
- HOGENOM:   HBG307581
- OMA:   FEGQVYV
- GO:   GO:0005737
- HAMAP:   MF_00118_B
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000
- PANTHER:   PTHR23115:SF31
- PRINTS:   PR00315
- TIGRFAMs:   TIGR00485
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3; SSF50465 Elong_init_C; SSF50447 Translat_factor

EC number: 3.6.5.3

Molecular weight: Translated: 44027; Mature: 43895

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKAKYERTKPHVNIGTIGHVDHGKTTLTAAITKTLYDRYQLGEAVDFANIDKAPEERER
CCCCCCCCCCCCEEECEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHC
GITISTAHVEYETPNRHYAHVDCPGHADYVKNMITGAAQMDGAILVCSATDGPMPQTREH
CCEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHH
ILLSRQVGVPYIVVFLNKCDMVDDEELLELVEMEVRDLLTEYDFPGDDTPIVRGSALMAL
HEEECCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCEEEEE
EDPKSEWGDKIVELFEQIDEYIPAPERDTDKPFLMPVEDVFSITGRGTVATGRVERGVLK
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHHHHHCCCCEEEECCHHCCEEE
VQDEVELVGLTEAPRKVVVTGVEMFRKLLDQAQAGDNIGALLRGVQRNEIERGQVLAKTG
ECCCEEEEECCCCCCCEEHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEEEECC
SVKAHTKFTAEVYVLKKEEGGRHTPFFDGYRPQFYFRTTDVTGACKLPEGIEMVMPGDNV
CEEEEEEEEEEEEEEEECCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCEEEECCCCC
TMEVDLINSIVVEEGLRFSIREGGRTVASGVVATIIE
EEEHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
AKAKYERTKPHVNIGTIGHVDHGKTTLTAAITKTLYDRYQLGEAVDFANIDKAPEERER
CCCCCCCCCCCEEECEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHC
GITISTAHVEYETPNRHYAHVDCPGHADYVKNMITGAAQMDGAILVCSATDGPMPQTREH
CCEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHH
ILLSRQVGVPYIVVFLNKCDMVDDEELLELVEMEVRDLLTEYDFPGDDTPIVRGSALMAL
HEEECCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCEEEEE
EDPKSEWGDKIVELFEQIDEYIPAPERDTDKPFLMPVEDVFSITGRGTVATGRVERGVLK
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHHHHHCCCCEEEECCHHCCEEE
VQDEVELVGLTEAPRKVVVTGVEMFRKLLDQAQAGDNIGALLRGVQRNEIERGQVLAKTG
ECCCEEEEECCCCCCCEEHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEEEECC
SVKAHTKFTAEVYVLKKEEGGRHTPFFDGYRPQFYFRTTDVTGACKLPEGIEMVMPGDNV
CEEEEEEEEEEEEEEEECCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCEEEECCCCC
TMEVDLINSIVVEEGLRFSIREGGRTVASGVVATIIE
EEEHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA